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LARP7 Gene

protein-coding   GIFtS: 49

GC04P113777
La ribonucleoprotein domain family, member 7
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DKFZP564K112 1
DKFZp564K112 2
HDCMA18P 1, 2
MGC104360 2
PIP7S 1, 2, 3, 5
Descriptions
La ribonucleoprotein domain family member 7 3
La ribonucleoprotein domain family, member 7 2
La-related protein 7 2
P-TEFb-interaction protein for 7SK stability 2, 3
External Ids
HGNC: 249121
Entrez Gene: 515742
UniProtKB: Q4G0J33
Ensembl: ENSG000001747207
Search outside databases for aliases for LARP7 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: LARP7_HUMAN, Q4G0J3
Function: Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP
system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is
sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent
transcriptional elongation

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LARP7 gene  

Entrez Gene cytogenetic band: 4q25   Ensembl cytogenetic band:  4q25   HGNC cytogenetic band: 4q25

LARP7 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P113777:     (about GC identifiers)

Start:
113,777,569 bp from pter
End:
113,798,191 bp from pter
Size:
20,623 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000004.10  NT_016354.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: LARP7_HUMAN, Q4G0J3 (See protein sequence)
Recommended Name: La-related protein 7  
Size: 582 amino acids; 66899 Da
Subunit: Integral part of the 7SK RNP complex. Specifically binds to the highly conserved
3'-terminal U-rich stretch of 7SK RNA. On stimulation, remains associated with 7SK RNA, whereas
P-TEFb is released from the complex
Subcellular location: Nucleus, nucleoplasm
Sequence caution: Sequence=ACD13786.1; Type=Erroneous initiation; Note=Translation N-terminally
shortened; Sequence=EAX06284.1; Type=Erroneous initiation; Note=Translation N-terminally
shortened;
Secondary accessions: B2ZHN6 Q3B7A9 Q9P1S7 Q9Y3Z8
Alternative splicing: 2 isoforms:  Q4G0J3-1   Q4G0J3-2   

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_056269.1  NP_057732.2  


    ENSEMBL proteins: 
    ENSP00000311306 ENSP00000378069 ENSP00000344950 ENSP00000314311 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (HDCMA18P)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0030529 ribonucleoprotein complex IEA--
    About this table

    Antibodies for LARP7: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for LARP7
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for LARP7

    Assays for LARP7: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR006630 Lupus_La_RNA_bd
     IPR011991 Wing_hlx_DNA_bd
     IPR002344 Lupus_La
     IPR014886 RRM_3
     IPR000504 RRM_RNP1


       GeneDecks  LARP7 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q4G0J3

    ProtoNet protein and cluster: Q4G0J3

    1 Blocks protein family: IPB002344 Lupus La protein signature

    UniProtKB/Swiss-Prot: LARP7_HUMAN, Q4G0J3
    Similarity: Contains 1 HTH La-type RNA-binding domain
    Similarity: Contains 1 RRM (RNA recognition motif) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LARP7)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_016648

                  Applied Biosystems Silencer® siRNAs for LARP7

                  Sigma-Aldrich siRNA for LARP7  
                         Sigma-Aldrich shRNA for LARP7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for LARP7
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_015454
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_015454
                                     untagged cDNA clones in CMV expression vector (see all 5): AK000274 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_016648

    UniProtKB/Swiss-Prot: LARP7_HUMAN, Q4G0J3
    Function: Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP
    system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is
    sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent
    transcriptional elongation

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003723 RNA binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  LARP7 


    4 Interacting proteins for LARP7 (ENSP000003449503 Q4G0J32) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CDK9P507502STRING (score=.938) MINT-6629492 MINT-6629521 MINT-6629505 MINT-6629599 MINT-6629464 MINT-6629479 MINT-6629534
    HEXIM1O949922STRING (score=.952) MINT-6629492 MINT-6629521 MINT-6629505 MINT-6629464 MINT-6629479 MINT-6629588 MINT-6629534
    CCNT1O605632STRING (score=.836) MINT-6629492 MINT-6629521 MINT-6629505 MINT-6629464 MINT-6629479 MINT-6629534
    CUEDC1ENSP000003533733STRING (score=.7)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006396 RNA processing IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LARP7

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LARP7)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_016648

                  Sigma-Aldrich siRNA for LARP7  
                         Sigma-Aldrich shRNA for LARP7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_015454  NM_016648  

    REFSEQ mRNAs for LARP7 gene (2 alternative transcripts): 

    NM_015454.1   NM_016648.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_015454  NM_016648  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_015454
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_015454
                                     untagged cDNA clones in CMV expression vector (see all 5): AK000274 

    Additional cDNA sequence: 

    AF068284.1 AK000089.1 AK000274.1 AK225900.1 AL049996.1 BC006981.1 BC066945.1 BC107709.1 
    BX647100.1 EU667388.1 

    24/27 DOTS entries (see all 27 ):

    DT.455967  DT.100826219  DT.95070005  DT.102833758  DT.102833759  DT.100826216  DT.95074509  DT.100826217 
    DT.121220526  DT.121220517  DT.40287376  DT.99998927  DT.101959098  DT.121220527  DT.91755773  DT.99976930 
    DT.100826221  DT.121220446  DT.424894  DT.121220493  DT.121220512  DT.86855559  DT.92001063  DT.97771568 

    24/246 AceView cDNA sequences (see all 246 ):

    AI635804 BX107395 AI811605 AW339069 BU676360 AW614164 AI478927 AI655849 
    AV692449 CA419668 CA420422 CB122680 AV690220 AA741383 BM311174 BF593364 
    AI276533 AW172889 BU784632 AA046636 AI670112 AI004357 AW514049 BQ048930 

    highest scoring ESTs for LARP7:

    AL049996 AI933958 AK000274 BC066945 BE748634 BX405367 BX647100 CA314598 CA416523 CA420422 

    Unigene Cluster for LARP7:

    La ribonucleoprotein domain family, member 7
    Hs.713663  [show with all ESTs]
    Unigene Representative Sequence: BC066945


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for LARP7 (see all 6 )

    ExUns: 1 ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
    SP1:                                                                          -                                             
    SP2:                          -                                                                                             
    SP3:                                                                                                                        
    SP4:                                                                                                                        
    SP5:                                                                                                                        

    About this scheme

    ECgene alternative splicing isoforms for LARP7

    4 Ensembl transcripts including schematic representations:
    ENST00000308878  ENST00000394568  ENST00000344442  ENST00000324052  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LARP7 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LARP7

    1 / 2 / 3

    6 probe-sets matching LARP7 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    78315_s_at2, 3 U95-E 1 1.00 1.00 0.97 0.97 AI742269 0.60 1.00 0.82 1
    35215_at2, 3 U95-A 1 0.75 1.00 0.88 1.03 AL049996 1.00 0.90 0.95 1

    212785_s_at2, 3 U133-A 1 1.00 1.00 -- -- AA160181 0.60 0.83 0.73 1

    215246_at*2, 3 U133-A 1 1.00 1.00 -- -- AK000089 0.40 1.00 0.76 1

    212785_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    215246_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  LARP7 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GACACAAGCA

    SOURCE GeneReport for Unigene cluster: Hs.713663
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LARP7 gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LARP71   -- La ribonucleoprotein domain family, member 7 86.7(n)
    86.7(a)
    478519  XM_535698.2  XP_535698.2 
    chimpanzee
    (Pan troglodytes)
    LARP71   -- La ribonucleoprotein domain family, member 7 99.37(n)
    99.31(a)
    738277  XM_001144338.1  XP_001144338.1 
    cow
    (Bos taurus)
    LARP71   -- La ribonucleoprotein domain family, member 7 90.77(n)
    90.18(a)
    505053  NM_001075184.1  NP_001068652.1 
    rat
    (Rattus norvegicus)
    Larp7l1   -- La ribonucleoprotein domain family, member 7-like 85.96(n)
    89.47(a)
    691210  XM_001077220.1  XP_001077220.1 
    mouse
    (Mus musculus)
    Larp71   -- La ribonucleoprotein domain family, member 7 81.54(n)
    82.89(a)
    28036  NM_138593.2  NP_613059.2 
    About this table        Species with no ortholog for LARP7

    ENSEMBL Gene Tree for LARP7
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LARP7 gene
    SSB2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/82 NCBI SNPs in LARP7 are shown (see all 82 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 23)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs126448681,2
    A,C,F,H113798611(+) AATCAC/TGGGAG 2 -- ng314Minor allele frequency- T:0.23EU EA WA 418
    rs11290651,2
    A,C,F,H113798058(+) TTCCTC/TAACTT 2 -- ut31 ese313Minor allele frequency- T:0.23MN EA NA EU WA 1228
    rs43836751,2
    A,C,F,H113792068(+) GAGACG/AGCTGA 2 -- int17Minor allele frequency- A:0.22EU EA WA NA 542
    --
    rs65336311,2
    A,C,F,H113797715(+) TTCTCG/ACTGTT 2 -- int17Minor allele frequency- A:0.14EU EA WA NA 552
    rs117250841,2
    C,F,H113782058(+) tttacG/Atattt 2 -- int12Minor allele frequency- A:0.02EU WA 240
    rs76770221,2
    C,F,H113795557(+) AGTTAA/GAAGTC 2 -- int14Minor allele frequency- G:0.16EU EA WA 418
    rs131367371,2
    C,F,H113788584(+) TTAACG/TCAATT 2 -- int110Minor allele frequency- T:0.35NA EU EA WA 1020
    rs76938951,2
    C,F,H113793233(+) AAAGTT/GGAATG 2 -- int14Minor allele frequency- G:0.01EU EA WA 416
    rs76568191,2
    F,H113790334(+) ACTTAT/CATATG 2 -- int14Minor allele frequency- C:0.17EU EA WA 410
    rs76957401,2
    F,H113794878(+) AAAATT/GTACTG 2 -- int14Minor allele frequency- G:0.12EU EA WA 414
    About this table

    HapMap Linkage Disequilibrium images for LARP7 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 612026

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for LARP7 gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 51574 HGNC: 24912 AceView: HDCMA18P Ensembl:ENSG00000174720 euGenes: HUgn51574
    ECgene: LARP7 H-InvDB: LARP7
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LARP7:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LARP7-related products

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     Predesigned and custom siRNAs for LARP7 Antibodies for LARP7
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LARP7 Browse small molecules at Sigma-Aldrich
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