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LAPTM5 Gene

protein-coding   GIFtS: 54

GC01M030999
lysosomal multispanning membrane protein 5
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CLAST6 2
FLJ97251 2
KIAA0085 3
MGC125860 2
MGC125861 2
OTTHUMP00000003806 2
Descriptions
CD40-ligand-activated specific transcripts 2
Lysosomal-associated multitransmembrane protein 5 3
Retinoic acid-inducible E3 protein 3
human retinoic acid-inducible E3 protein 2
lysosomal associated multispanning membrane protein 5 2
lysosomal multispanning membrane protein 5 2
External Ids
HGNC: 296121
Entrez Gene: 78052
UniProtKB: Q135713
Ensembl: ENSG000001625117
Search outside databases for aliases for LAPTM5 gene

Previous GC identifers: GC01M030189 GC01M030631 GC01M030707 GC01M030700 GC01M030874

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for LAPTM5:
This gene encodes a transmembrane receptor that is associated with lysosomes. The encoded protein,
also known as E3 protein, may play a role in hematopoiesis. [provided by RefSeq]

UniProtKB/Swiss-Prot: LAPM5_HUMAN, Q13571
Function: May have a special functional role during embryogenesis and in adult hematopoietic cells

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LAPTM5 gene  

Entrez Gene cytogenetic band: 1p34   Ensembl cytogenetic band:  1p35.2   HGNC cytogenetic band: 1p34

LAPTM5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M030999:     (about GC identifiers)

Start:
30,977,903 bp from pter
End:
31,003,254 bp from pter
Size:
25,352 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_032977.8  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: LAPM5_HUMAN, Q13571 (See protein sequence)
Recommended Name: Lysosomal-associated transmembrane protein 5  
Size: 262 amino acids; 29937 Da
Subunit: Binds to ubiquitin
Subcellular location: Lysosome membrane; Multi-pass membrane protein
Secondary accessions: Q13240 Q14698 Q3KP54

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006753.1  

    ENSEMBL proteins: 
    ENSP00000294507 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (LAPTM5)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005765 lysosomal membrane IEA--
    GO:0005887 integral to plasma membrane TAS8661146
    GO:0016020 membrane IEA--
    About this table

    Antibodies for LAPTM5: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for LAPTM5

    Assays for LAPTM5: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR004687 Mtp_transporter
     IPR018396 Mtp_transporter_mml
     IPR018397 Lysosomal-assoc_TM_prot5


       GeneDecks  LAPTM5 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q13571

    ProtoNet protein and cluster: Q13571

    1 Blocks protein family: IPB004687 Golgi 4-transmembrane spanning transporter

    UniProtKB/Swiss-Prot: LAPM5_HUMAN, Q13571
    Similarity: Belongs to the LAPTM4/LAPTM5 transporter family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LAPTM5)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006762

                  Applied Biosystems Silencer® siRNAs for LAPTM5

                  Sigma-Aldrich siRNA for LAPTM5  
                         Sigma-Aldrich shRNA for LAPTM5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006762
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006762
                                     untagged cDNA clone in CMV expression vector: NM_006762 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006762

    UniProtKB/Swiss-Prot: LAPM5_HUMAN, Q13571
    Function: May have a special functional role during embryogenesis and in adult hematopoietic cells
    Induction: By retinoic acid

    3 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Laptm5):

    hematopoietic systemimmune systemno phenotypic analysis

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/18 Interacting proteins for LAPTM5 (ENSP000002945073 Q135712) via UniProtKB, MINT, and/or STRING (see all 18 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NEDD4P469342STRING (score=.93) MINT-62181
    ANKRD13AQ8IZ072MINT-62174 MINT-62173
    SMURF2Q9HAU42MINT-61804 MINT-62170
    DCUN1D1Q96GG92MINT-62171
    EPN1Q9Y6I32MINT-62183
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006810 transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LAPTM5

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LAPTM5)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006762

                  Sigma-Aldrich siRNA for LAPTM5  
                         Sigma-Aldrich shRNA for LAPTM5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006762  

    REFSEQ mRNAs for LAPTM5 gene: 

    NM_006762.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006762  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006762
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006762
                                     untagged cDNA clone in CMV expression vector: NM_006762 

    Additional cDNA sequence: 

    AK292009.1 AK297014.1 BC106896.1 BC106897.1 CR607333.1 CR626282.1 D42042.1 U30498.1 
    U51240.1 

    12 DOTS entries:

    DT.447574  DT.92438025  DT.100809473  DT.121407327  DT.100840534  DT.121407323  DT.121407335  DT.95209605 
    DT.100715378  DT.121407347  DT.87016466  DT.91794966 

    24/366 AceView cDNA sequences (see all 366 ):

    AI453635 AW059657 AA479762 AI361789 AA022995 AA382106 BM668793 BG393652 
    AW575972 CR607333 AW402266 AW406311 AA586871 AW574642 F12601 CD369983 
    AA583177 CK822044 BF001471 AW513037 AW402135 CA748549 CF242935 BU616667 

    highest scoring ESTs for LAPTM5:

    U51240 AA220214 AW404191 AW406175 BE244130 BE245144 BF663787 BF877233 BG341213 BG393652 

    Unigene Cluster for LAPTM5:

    Lysosomal multispanning membrane protein 5
    Hs.371021  [show with all ESTs]
    Unigene Representative Sequence: NM_006762


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for LAPTM5

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c · 9d
    SP1:              -           -                             -                                 
    SP2:                          -                             -                                 
    SP3:              -     -     -                             -                                 
    SP4:                                                        -                                 
    SP5:                                                                                          

    About this scheme

    ECgene alternative splicing isoforms for LAPTM5

    1 Ensembl transcript including schematic representation:
    ENST00000294507  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LAPTM5 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LAPTM5

    1 / 2 / 3

    5 probe-sets matching LAPTM5 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    37759_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 U51240 1.00 1.00 1.00 1

    201721_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006762 0.60 1.00 0.82 1

    201720_s_at2, 3 U133-A 1 1.00 1.00 -- -- AI589086 0.60 1.00 0.82 1

    201721_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    201720_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  LAPTM5 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCGGTTGTGG

    SOURCE GeneReport for Unigene cluster: Hs.371021

    Expression variation in blood from EXPOLDB for LAPTM5

    UniProtKB/Swiss-Prot: LAPM5_HUMAN, Q13571
    Tissue specificity: Preferentially expressed in adult hematopoietic tissues. High levels in
    lymphoid and myeloid tissues. Highly expressed in peripheral blood leokocytes, thymus, spleen and
    lung, followed by placenta, liver and kidney

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LAPTM5 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LAPTM51   -- lysosomal multispanning membrane protein 5 88.42(n)
    85.11(a)
    487324  XM_544450.2  XP_544450.2 
    chimpanzee
    (Pan troglodytes)
    LAPTM51   -- lysosomal multispanning membrane protein 5 99.24(n)
    98.85(a)
    456693  XM_513264.2  XP_513264.2 
    cow
    (Bos taurus)
    LAPTM51   -- lysosomal multispanning membrane protein 5 89.44(n)
    88.17(a)
    512361  NM_001046118.1  NP_001039583.1 
    rat
    (Rattus norvegicus)
    Laptm51   -- lysosomal multispanning membrane protein 5 80.72(n)
    80.08(a)
    89783  NM_053538.2  NP_445990.2 
    mouse
    (Mus musculus)
    Laptm51, 5 45
    lysosomal-associated protein transmembrane 51, 5 81.1(n)1
    79.31(a)1
    167921  NM_010686.21  NP_034816.11 
     AF3640505  AK0318025  (see all 53)
    About this table        Species with no ortholog for LAPTM5

    ENSEMBL Gene Tree for LAPTM5
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/162 NCBI SNPs in LAPTM5 are shown (see all 162 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 106)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs14078811,2
    A,C,F,H,O31004863(-) TCAAAT/CGGCTT 1 -- ng3120Minor allele frequency- C:0.08EA NA EU WA 1640
    rs80481,2
    A,C,F,H30978383(+) CACTGC/TTATGC 1 -- ut31 ese38Minor allele frequency- T:0.39MN EA EU WA 2042
    rs118016291,2
    A,C,F,H31003713(+) CCTTTC/GATCCT 1 -- ng314Minor allele frequency- G:0.35EU EA WA 420
    rs37622971,2
    A,C,F,H31004267(+) AAGTAC/TATGCA 1 -- ng319Minor allele frequency- T:0.19EA EU WA 1910
    rs13234441,2
    C,F,H,O31004663(-) TGTCAC/ACTTTC 1 -- ng3110Minor allele frequency- A:0.01EA NA EU WA 624
    rs109141931,2
    A,C,F,H31003920(+) AGGGAA/GAAGCT 1 -- ng318Minor allele frequency- N:0.00EU EA WA NA 634
    --
    rs37622961,2
    A,C,F,H31003973(+) AGGACA/GGAAGA 1 -- ng31 tfbs36Minor allele frequency- G:0.45EU EA NA 440
    rs118080671,2
    C,F,H31004457(+) TAAGCT/ACCTCC 1 -- ng314Minor allele frequency- A:0.01EU EA WA 410
    rs353512921,2
    C,F30980629(-) GAAGAG/AAAACT 1 K/R mis15Minor allele frequency- A:0.12NA EU EA WA 492
    rs37954391,2
    F,H30978277(-) GAATCT/GGTTCT 1 -- ut314Minor allele frequency- G:0.00EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for LAPTM5 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601476

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10 PubMed articles for LAPTM5 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 7805 HGNC: 29612 AceView: LAPTM5 Ensembl:ENSG00000162511 euGenes: HUgn7805
    ECgene: LAPTM5 H-InvDB: LAPTM5
    (According to HUGE)
    About This Section

    HUGE: KIAA0085
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LAPTM5:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LAPTM5-related products

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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for LAPTM5 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LAPTM5 Browse small molecules at Sigma-Aldrich
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