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LAPTM4A Gene

protein-coding   GIFtS: 50

GC02M020154
lysosomal protein transmembrane 4 alpha
(Previous name: lysosomal-associated protein transmembrane 4 alpha )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: MBNT)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
HUMORF13 1, 2
KIAA0108 1, 2, 3
LAPTM4 1, 2, 3
MBNT 2, 3
MTRP 3
Mtrp 1, 2
Descriptions
Golgi 4-transmembrane spanning transporter MTP 3
lysosomal protein transmembrane 4 alpha 2
lysosomal-associated protein transmembrane 4 alpha 1, 2
membrane nucleoside transporter 2
External Ids
HGNC: 69241
Entrez Gene: 97412
UniProtKB: Q150123
Ensembl: ENSG000000686977
Search outside databases for aliases for LAPTM4A gene

Previous GC identifers: GC02M020123 GC02M020300 GC02M020216

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for LAPTM4A:
This gene encodes a protein that has four predicted transmembrane domains. The function of this
gene has not yet been determined; however, studies in the mouse homolog suggest a role in the
transport of small molecules across endosomal and lysosomal membranes. [provided by RefSeq]

UniProtKB/Swiss-Prot: LAP4A_HUMAN, Q15012
Function: May function in the transport of nucleosides and/or nucleoside derivatives between the
cytosol and the lumen of an intracellular membrane-bound compartment (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LAPTM4A gene  

Entrez Gene cytogenetic band: 2p24.1   Ensembl cytogenetic band:  2p24.1   HGNC cytogenetic band: 2p24.1

LAPTM4A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M020154:     (about GC identifiers)

Start:
20,095,894 bp from pter
End:
20,114,926 bp from pter
Size:
19,033 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_015926.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LAP4A_HUMAN, Q15012 (See protein sequence)
Recommended Name: Lysosomal-associated transmembrane protein 4A  
Size: 233 amino acids; 26801 Da
Subcellular location: Endomembrane system; Multi-pass membrane protein (Potential). Note=May reside
in an intracellular membrane-bound compartment (Potential)
Sequence caution: Sequence=AAQ89387.1; Type=Frameshift; Positions=206;
Secondary accessions: Q6UW22

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_055528.1  

    ENSEMBL proteins: 
    ENSP00000175091 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (LAPTM4A)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005794 Golgi apparatus IDA10942595
    GO:0012505 endomembrane system IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for LAPTM4A: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for LAPTM4A
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for LAPTM4A

    Assays for LAPTM4A: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR004687 Mtp_transporter
     IPR018396 Mtp_transporter_mml


       GeneDecks  LAPTM4A for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q15012

    ProtoNet protein and cluster: Q15012

    1 Blocks protein family: IPB004687 Golgi 4-transmembrane spanning transporter

    UniProtKB/Swiss-Prot: LAP4A_HUMAN, Q15012
    Domain: The C-terminal domain is necessary for retention within intracelllular membranes (By
    similarity)
    Similarity: Belongs to the LAPTM4/LAPTM5 transporter family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LAPTM4A)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014713

                  Applied Biosystems Silencer® siRNAs for LAPTM4A

                  Sigma-Aldrich siRNA for LAPTM4A  
                         Sigma-Aldrich shRNA for LAPTM4A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for LAPTM4A
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014713
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014713
                                     untagged cDNA clone in CMV expression vector: NM_014713 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_014713

    UniProtKB/Swiss-Prot: LAP4A_HUMAN, Q15012
    Function: May function in the transport of nucleosides and/or nucleoside derivatives between the
    cytosol and the lumen of an intracellular membrane-bound compartment (By similarity)

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006810 transport IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LAPTM4A
    1 Novoseek chemical compound relationship for LAPTM4A gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    nucleoside 67.31 4 17345146 (1), 18359514 (1), 10037000 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LAPTM4A)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014713

                  Sigma-Aldrich siRNA for LAPTM4A  
                         Sigma-Aldrich shRNA for LAPTM4A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014713  

    REFSEQ mRNAs for LAPTM4A gene: 

    NM_014713.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014713  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014713
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014713
                                     untagged cDNA clone in CMV expression vector: NM_014713 

    Additional cDNA sequence: 

    AK129875.1 AK304433.1 AY359028.1 BC000421.2 BC003158.1 BC093075.1 CR456758.1 CR594890.1 
    CR597537.1 CR599013.1 CR599755.1 CR602246.1 CR604791.1 CR604820.1 CR605678.1 CR607529.1 
    CR607775.1 CR607830.1 CR608072.1 CR611436.1 CR612473.1 CR613521.1 CR615552.1 CR618524.1 
    CR618630.1 CR619265.1 CR621011.1 CR621170.1 CR622236.1 CR623001.1 CR624947.1 D14696.1 

    24/39 DOTS entries (see all 39 ):

    DT.85104883  DT.100699105  DT.100640749  DT.100045326  DT.100699087  DT.91871506  DT.95363945  DT.100699110 
    DT.100799582  DT.92012889  DT.121034068  DT.121033998  DT.95211206  DT.95363985  DT.100699088  DT.121034059 
    DT.91987790  DT.100699090  DT.100699117  DT.121034072  DT.91778869  DT.100699097  DT.100699107  DT.100699116 

    24/1386 AceView cDNA sequences (see all 1386 ):

    CR597537 AI560796 BQ883726 AL583634 BG701441 BQ677577 BM994108 BM771159 
    AW275242 BP375651 AI687325 R60939 CR624947 BU616856 AA149492 AI240867 
    AW513584 BM678432 AA382820 BM888345 AW411242 W57820 AI744902 AA370515 

    highest scoring ESTs for LAPTM4A:

    D14696 AA027778 AA053657 AA093846 AA121281 AA206647 AA207212 AA214362 AA300283 AA300717 

    Unigene Cluster for LAPTM4A:

    Lysosomal protein transmembrane 4 alpha
    Hs.467807  [show with all ESTs]
    Unigene Representative Sequence: NM_014713


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for LAPTM4A

    ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8a · 8b
    SP1:                                                                                          
    SP2:                                                                                          
    SP3:                                      -                                                   
    SP4:        -     -     -     -                                                               
    SP5:                                            -                                             

    About this scheme

    ECgene alternative splicing isoforms for LAPTM4A

    1 Ensembl transcript including schematic representation:
    ENST00000175091  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LAPTM4A expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LAPTM4A

    1 / 2 / 3

    3 probe-sets matching LAPTM4A gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    39019_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D14696 1.00 1.00 1.00 1

    200673_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014713 0.60 1.00 0.82 1

    200673_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TTTCTAGTTT

    SOURCE GeneReport for Unigene cluster: Hs.467807

    Expression variation in blood from EXPOLDB for LAPTM4A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LAPTM4A gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LAPTM4A1   -- lysosomal protein transmembrane 4 alpha 93.85(n)
    98.28(a)
    475678  XM_532885.2  XP_532885.1 
    chimpanzee
    (Pan troglodytes)
    LAPTM4A1   -- lysosomal protein transmembrane 4 alpha 99.71(n)
    100(a)
    459051  XM_001138869.1  XP_001138869.1 
    cow
    (Bos taurus)
    LAPTM4A1   -- lysosomal protein transmembrane 4 alpha 93.56(n)
    98.28(a)
    404135  NM_205799.1  NP_991368.1 
    rat
    (Rattus norvegicus)
    Laptm4a1   -- lysosomal protein transmembrane 4 alpha 92.85(n)
    97.85(a)
    298875  NM_199384.1  NP_955416.1 
    mouse
    (Mus musculus)
    Laptm4a1, 5 125
    lysosomal-associated protein transmembrane 4A1, 5 92.13(n)1
    97.42(a)1
    177751  NM_008640.21  NP_032666.21 
     AA2864665  AK0139635  (see all 22)
    About this table        Species with no ortholog for LAPTM4A

    ENSEMBL Gene Tree for LAPTM4A
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LAPTM4A gene
    LAPTM4B2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/96 NCBI SNPs in LAPTM4A are shown (see all 96 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 64)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs11918251,2
    A,C,F,H20095768(-) ccttcG/Atatcc 1 -- ng518Minor allele frequency- A:0.32EU EA WA 838
    rs37316731,2
    C,F,H20104149(+) TCAGAC/AGAATA 1 S/S syn1 ese36Minor allele frequency- A:0.14MN EU EA WA NA 676
    rs37697601,2
    A,C,F,H20111640(+) AATACG/CAAAGG 1 -- int117Minor allele frequency- C:0.21EA NA EU WA 2870
    rs11918241,2
    A,C,F,H20096616(-) TGTATA/GACCCT 1 -- int18Minor allele frequency- G:0.35EU EA WA NA 762
    rs11471161,2
    A,C,F,H20099811(-) AATTCG/ATCATT 1 -- int116Minor allele frequency- A:0.42EA NA EU WA 1492
    rs67063751,2
    A,C,F,H20112908(+) AAATAG/TAAATG 1 -- int14Minor allele frequency- T:0.16EU EA WA 418
    rs10163161,2
    A,C,F,H20098590(+) GCCTTT/CGGAAA 1 -- int18Minor allele frequency- C:0.26EU EA WA 838
    rs130290561,2
    A,C,F,H20106039(+) GTTAAT/CGACTC 1 -- int18Minor allele frequency- C:0.48EU EA WA NA 780
    rs37697621,2
    A,C,F,H20113310(+) GTTCTG/ATCAAT 1 -- int18Minor allele frequency- A:0.22EA NA EU WA 2050
    rs13989511,2
    C,F,H,O20112749(+) CGCCCC/GTAATG 1 -- int113Minor allele frequency- N:0.00EA NA EU WA 2502
    About this table

    HapMap Linkage Disequilibrium images for LAPTM4A (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/14 PubMed articles for LAPTM4A gene (see all 14 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9741 HGNC: 6924 AceView: LAPTM4A Ensembl:ENSG00000068697 euGenes: HUgn9741
    ECgene: LAPTM4A H-InvDB: LAPTM4A
    (According to HUGE)
    About This Section

    HUGE: KIAA0108
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
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      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LAPTM4A:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LAPTM4A-related products

     Millipore Custom Antibody & Bulk Services
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     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for LAPTM4A Antibodies for LAPTM4A
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LAPTM4A Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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