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LAMC3 Gene

protein-coding   GIFtS: 54

GC09P132874
laminin, gamma 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DKFZp434E202 1, 2
OTTHUMP00000064565 2
Descriptions
Laminin 12 gamma 3 subunit 3
laminin, gamma 3 2
External Ids
HGNC: 64941
Entrez Gene: 103192
UniProtKB: Q9Y6N63
Ensembl: ENSG000000505557
Search outside databases for aliases for LAMC3 gene

Previous GC identifers: GC09P124899 GC09P125431 GC09P127238 GC09P129160 GC09P130914

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for LAMC3:
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent
of basement membranes. They have been implicated in a wide variety of biological processes
including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis.
Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1,
and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed
by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain
protein encoded by a distinct gene. Several isoforms of each chain have been described. Different
alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin
isoforms which are designated by Arabic numerals in the order of their discovery, i.e.
alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and
trimer molecules are largely unknown, but some of the chains have been shown to differ with
respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene
encodes the gamma chain isoform laminin, gamma 3. The gamma 3 chain is most similar to the gamma 1
chain, and contains all the 6 domains expected of the gamma chain. It is a component of laminin
12. The gamma 3 chain is broadly expressed in skin, heart, lung, and the reproductive tracts. In
skin, it is seen within the basement membrane of the dermal-epidermal junction at points of nerve
penetration. However, it was also found that the gamma 3 is a prominent element of the apical
surface of ciliated epithelial cells of lung, oviduct, epididymis, ductus deferens, and
seminiferous tubules. The distribution of gamma 3-containing laminins along ciliated epithelial
surfaces suggests that the apical laminins are important in the morphogenesis and structural
stability of the ciliated processes of these cells. [provided by RefSeq]

UniProtKB/Swiss-Prot: LAMC3_HUMAN, Q9Y6N6
Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the
attachment, migration and organization of cells into tissues during embryonic development by
interacting with other extracellular matrix components

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LAMC3 gene  

Entrez Gene cytogenetic band: 9q31-q34   Ensembl cytogenetic band:  9q34.13   HGNC cytogenetic band: 9q31-q34

LAMC3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P132874:     (about GC identifiers)

Start:
132,874,325 bp from pter
End:
132,958,267 bp from pter
Size:
83,943 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000009.10  NT_035014.4  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LAMC3_HUMAN, Q9Y6N6 (See protein sequence)
Recommended Name: Laminin subunit gamma-3 precursor  
Size: 1587 amino acids; 172458 Da
Subunit: Laminin is a complex glycoprotein, consisting of three different polypeptide chains
(alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped
molecule comprising one long and three short arms with globules at each end. Gamma-3 is a subunit
of laminin-12
Subcellular location: Secreted, extracellular space, extracellular matrix, basement membrane
Sequence caution: Sequence=AAD36991.1; Type=Frameshift; Positions=598, 609;
Secondary accessions: B1APX9

REFSEQ proteins: NP_006050.3  

ENSEMBL proteins: 
ENSP00000325873 ENSP00000347627 ENSP00000354360 ENSP00000347156 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Laminin)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
GO:0005604 basement membrane IEA--
GO:0016020 membrane TAS10225960
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Antibodies for LAMC3: 
Invitrogen Antibodies for LAMC3
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (Laminin), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for LAMC3

Assays for LAMC3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR000034 Laminin_B_type_IV
 IPR008211 Laminin_N
 IPR013032 EGF-like_reg_CS
 IPR002049 EGF_laminin


   GeneDecks  LAMC3 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9Y6N6

ProtoNet protein and cluster: Q9Y6N6

3 Blocks protein families:
IPB000034 Laminin B
IPB002049 Laminin-type EGF-like domain
IPB008212 Laminin


UniProtKB/Swiss-Prot: LAMC3_HUMAN, Q9Y6N6
Domain: The alpha-helical domains I and II are thought to interact with other laminin chains to
form a coiled coil structure
Domain: Domain IV is globular
Similarity: Contains 11 laminin EGF-like domains
Similarity: Contains 1 laminin IV type A domain
Similarity: Contains 1 laminin N-terminal domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (LAMC3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006059

              Applied Biosystems Silencer® siRNAs for LAMC3

              Sigma-Aldrich siRNA for LAMC3  
                     Sigma-Aldrich shRNA Panels and shRNA for LAMC3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006059
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_006059
                                 untagged cDNA clone in CMV expression vector: NM_006059 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_006059

UniProtKB/Swiss-Prot: LAMC3_HUMAN, Q9Y6N6
Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the
attachment, migration and organization of cells into tissues during embryonic development by
interacting with other extracellular matrix components

Genatlas biochemistry entry for LAMC3:
laminin ,gamma 3 polypeptide,prominent element of the apical surface of cialiated epithelial cells
of lung,oviduct,epididymis,ductus deferens,seminiferous tubules,also component of cutaneous
basement membrane zone,heterotrimerizing (laminin 12) with alpha 2 and beta 1

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005198 structural molecule activity TAS10225960
GO:0005515 protein binding IEA--
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(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for LAMC3 (Maps provided by GeneGo):
 Integrin-mediated cell-matrix adhesion

   GeneDecks  LAMC3 for the pathways selected above  
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  LAMC3  (Your Favorite Gene powered by Ingenuity) 
 Germ Cell-Sertoli Cell Junction Signaling

   GeneDecks  LAMC3 for the pathways selected above  
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4 Kegg Pathways  (Kegg details for LAMC3):
 hsa04510 Focal adhesion
 hsa04512 ECM-receptor interaction
 hsa05200 Pathways in cancer
 hsa05222 Small cell lung cancer

   GeneDecks  LAMC3 for the pathways selected above  
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1 Interacting protein for LAMC3 (Q9Y6N62) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
--Q9WMX22MINT-7048620
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007155 cell adhesion IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for LAMC3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (LAMC3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006059

              Sigma-Aldrich siRNA for LAMC3  
                     Sigma-Aldrich shRNA Panels and shRNA for LAMC3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_006059  

REFSEQ mRNAs for LAMC3 gene: 

NM_006059.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_006059  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006059
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_006059
                                 untagged cDNA clone in CMV expression vector: NM_006059 

Additional cDNA sequence: 

AB208887.1 AK075536.1 AL133595.1 CR618197.1 

2 DOTS entries:

DT.91961659  DT.312334 

24/83 AceView cDNA sequences (see all 83 ):

D31213 CR618197 NM_006059 AA909401 T35029 BU754408 AA778091 BU790210 
AF041835 AI290116 BX089329 CD655221 BE145399 AL110446 BI161423 AA985315 
CA444538 CA433082 BQ027531 AW368696 BQ643963 BU729072 AL548827 BF343520 

highest scoring ESTs for LAMC3:

AI025311 AL133595 AL573239 AL575168 AW368696 BM671064 BM673182 BM685344 BQ027531 R24217 

Unigene Cluster for LAMC3:

Laminin, gamma 3
Hs.201805  [show with all ESTs]
Unigene Representative Sequence: NM_006059


GeneLoc Exon Structure

4 Ensembl transcripts including schematic representations:
ENST00000320021  ENST00000355452  ENST00000361069  ENST00000355048  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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LAMC3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for LAMC3

1 / 2 / 3

5 probe-sets matching LAMC3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

62549_at2, 3 U95-C 1 1.00 1.00 0.78 0.74 AI025311 0.80 1.00 0.91 1
91561_at2, 3 U95-E 1 1.00 1.00 0.65 1.04 AL049143 0.40 1.00 0.76 1
44760_at2, 3 U95-B 1 1.00 1.00 0.67 1.22 R45355 0.60 1.00 0.82 1

219407_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006059 0.60 1.00 0.82 1

219407_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  LAMC3 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CTGGCCTCTG

SOURCE GeneReport for Unigene cluster: Hs.201805

UniProtKB/Swiss-Prot: LAMC3_HUMAN, Q9Y6N6
Tissue specificity: Broadly expressed in: skin, heart, lung, and the reproductive tracts

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for LAMC3 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
LAMC31   -- laminin, gamma 3 84.71(n)
81.93(a)
491290  XM_548411.2  XP_548411.2 
cow
(Bos taurus)
LAMC31   -- laminin, gamma 3 85.5(n)
83.59(a)
518914  XM_597117.3  XP_597117.3 
rat
(Rattus norvegicus)
Lamc31   -- laminin gamma 3 78.74(n)
75.99(a)
311862  XM_231139.4  XP_231139.4 
mouse
(Mus musculus)
Lamc31, 5 25
laminin gamma 31, 5 79.01(n)1
75.72(a)1
239281  NM_011836.31  NP_035966.21 
 AF0795205  AF0833725  (see all 16)
chicken
(Gallus gallus)
LAMC31   -- laminin, gamma 3 66.31(n)
61.38(a)
417180  XM_415462.2  XP_415462.2 
About this table        Species with no ortholog for LAMC3

ENSEMBL Gene Tree for LAMC3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for LAMC3 gene
LAMC12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/708 NCBI SNPs in LAMC3 are shown (see all 708 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 201)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 9 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs7100741,2
A,C,F,H,O132957116(-) CGGGGT/GCGAGT 1 -- ut31 ese35Minor allele frequency- G:0.38MN EU EA WA 604
rs7529281,2
A,C,F,H,O132958433(-) CCAGCG/AAAGCC 1 -- ng315Minor allele frequency- A:0.40MN EU EA WA 600
rs7529291,2
A,C,F,H,O132958367(-) AACGTC/TCGGTC 1 -- ng3117Minor allele frequency- N:0.00MN EU EA WA NA 1656
rs7100751,2
A,C,F,H,O132957649(-) CTGCTG/AAGAAC 1 -- ut31 ese34Minor allele frequency- A:0.46NA EU WA 404
rs8180531,2
A,C,F,H,O132958571(-) GGCTGT/AGAGTT 1 -- ng315Minor allele frequency- A:0.41MN EU EA WA 596
rs7481011,2
A,C,F,H,O132957596(+) AGCTCG/AACACA 1 -- ut315Minor allele frequency- A:0.09MN EA NA EU 1984
rs22751401,2
A,C,F,H,O132937870(+) TGATGG/AGCCTC 1 G/S mis110Minor allele frequency- A:0.30EA NA EU WA 2234
rs47404121,2
A,C,F,H132952829(+) TGAGCG/AGGTAC 1 Q/R mis17Minor allele frequency- A:0.12NA EU EA WA 558
rs7529301,2
A,F,H,O132958328(-) ATATGC/TACACC 1 -- ng315Minor allele frequency- T:0.21MN EU EA WA 602
rs7100761,2
A,C,F,H132957722(-) CAGGAA/GTGGGC 1 -- ut31 ese31Minor allele frequency- G:0.29WA 120
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HapMap Linkage Disequilibrium images for LAMC3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604349

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

7 PubMed articles for LAMC3 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 10319 HGNC: 6494 AceView: LAMC3 Ensembl:ENSG00000050555 euGenes: HUgn10319
ECgene: LAMC3 H-InvDB: LAMC3
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for LAMC3 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

Licensable Technologies for LAMC3 gene:
 Tufts University:  Laminins and their functions in nervous systems

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for LAMC3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Lentivirus-delivered shRNAs for LAMC3 Browse small molecules at Sigma-Aldrich
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 Recombinant Proteins
(Laminin)
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 Search Novus for antibodies for LAMC3

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