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LAMB4 Gene

protein-coding   GIFtS: 47

GC07M107451
laminin, beta 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Descriptions
Laminin beta-1-related protein 3
laminin, beta 4 2
External Ids
HGNC: 64911
Entrez Gene: 227982
UniProtKB: A4D0S43
Ensembl: ENSG000000911287
Search outside databases for aliases for LAMB4 gene

Previous GC identifers: GC07M106148 GC07M107210 GC07M107224 GC07M107257

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: LAMB4_HUMAN, A4D0S4
Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the
attachment, migration and organization of cells into tissues during embryonic development by
interacting with other extracellular matrix components

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LAMB4 gene  

Entrez Gene cytogenetic band: 7q22-q31.2   Ensembl cytogenetic band:  7q31.1   HGNC cytogenetic band: 7q31

LAMB4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M107451:     (about GC identifiers)

Start:
107,451,232 bp from pter
End:
107,558,037 bp from pter
Size:
106,806 bases
Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 107,025,127-107,131,988     
RefSeq DNA sequence:
NC_000007.12  NT_007933.14  NT_079596.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LAMB4_HUMAN, A4D0S4 (See protein sequence)
Recommended Name: Laminin subunit beta-4 precursor  
Size: 1761 amino acids; 193540 Da
Subunit: Laminin is a complex glycoprotein, consisting of three different polypeptide chains
(alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped
molecule comprising one long and three short arms with globules at each end
Subcellular location: Secreted, extracellular space, extracellular matrix, basement membrane
Secondary accessions: A5PKU6 B2RTT3 Q86TP7 Q86XN2 Q8NBX5
Alternative splicing: 4 isoforms:  A4D0S4-1   A4D0S4-2   A4D0S4-3   A4D0S4-4   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_031382.2  

    ENSEMBL proteins: 
    ENSP00000373432 ENSP00000373433 ENSP00000205386 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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    Browse Abcam for Recombinant Proteins
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005604 basement membrane IEA--
    About this table

    Antibodies for LAMB4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for LAMB4

    Assays for LAMB4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR013015 Laminin_IV
     IPR008211 Laminin_N
     IPR013032 EGF-like_reg_CS
     IPR002049 EGF_laminin


       GeneDecks  LAMB4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry A4D0S4

    ProtoNet protein and cluster: A4D0S4

    UniProtKB/Swiss-Prot: LAMB4_HUMAN, A4D0S4
    Domain: The alpha-helical domains I and II are thought to interact with other laminin chains to
    form a coiled coil structure
    Domain: Domains VI and IV are globular
    Similarity: Contains 13 laminin EGF-like domains
    Similarity: Contains 1 laminin IV type B domain
    Similarity: Contains 1 laminin N-terminal domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LAMB4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_007356

                  Applied Biosystems Silencer® siRNAs for LAMB4

                  Sigma-Aldrich siRNA for LAMB4  
                         Sigma-Aldrich shRNA Panels and shRNA for LAMB4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_007356
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_007356
                                     untagged cDNA clone in CMV expression vector: NM_007356 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_007356

    UniProtKB/Swiss-Prot: LAMB4_HUMAN, A4D0S4
    Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the
    attachment, migration and organization of cells into tissues during embryonic development by
    interacting with other extracellular matrix components

    Genatlas biochemistry entry for LAMB4:
    laminin,beta 4,with two alternatively spliced

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    4 Kegg Pathways  (Kegg details for LAMB4):
     hsa04510 Focal adhesion
     hsa04512 ECM-receptor interaction
     hsa05200 Pathways in cancer
     hsa05222 Small cell lung cancer

       GeneDecks  LAMB4 for the pathways selected above  
    About GeneDecksing

    5/90 Interacting proteins for LAMB4 (ENSP000003734323) via UniProtKB, MINT, and/or STRING (see all 90 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EGFENSP000002651713STRING (score=.953)
    CDH1ENSP000002687943STRING (score=.951)
    COL17A1ENSP000003409373STRING (score=.932)
    ITGA6ENSP000002641063STRING (score=.93)
    LAMA2ENSP000003507173STRING (score=.914)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007155 cell adhesion IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for LAMB4

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (LAMB4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_007356

                  Sigma-Aldrich siRNA for LAMB4  
                         Sigma-Aldrich shRNA Panels and shRNA for LAMB4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_007356  

    REFSEQ mRNAs for LAMB4 gene: 

    NM_007356.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_007356  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_007356
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_007356
                                     untagged cDNA clone in CMV expression vector: NM_007356 

    Additional cDNA sequence: 

    AF028816.1 AK300385.1 AK301777.1 BC045172.2 BC140804.1 BC142619.1 BC144591.1 

    6 DOTS entries:

    DT.40113979  DT.100698738  DT.100736500  DT.40114466  DT.91700592  DT.100009859 

    14 AceView cDNA sequences:

    NM_007356 BC045172 BX095174 AF028816 AI803176 BI459439 BG480015 W69706 
    BI461103 AF029325 BG216241 W69707 BV193712 AK075165 

    highest scoring ESTs for LAMB4:

    AF028816 AF029325 BC045172 BG216241 BG480015 NM_007356 W69707 AI803176 BI459439 BI461103 

    Unigene Cluster for LAMB4:

    Laminin, beta 4
    Hs.62022  [show with all ESTs]
    Unigene Representative Sequence: AF028816


    GeneLoc Exon Structure

    3 Ensembl transcripts including schematic representations:
    ENST00000388780  ENST00000388781  ENST00000205386  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    LAMB4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for LAMB4

    1 / 2 / 3

    7 probe-sets matching LAMB4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    31613_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF029325 0.60 1.00 0.82 1

    216622_at2, 3 U133-A 1 1.00 1.00 -- -- AF029325 0.60 1.00 0.82 1

    234334_s_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    215516_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    216622_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    234334_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    215516_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GATAAAAATA

    SOURCE GeneReport for Unigene cluster: Hs.62022

    Expression variation in blood from EXPOLDB for LAMB4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for LAMB4 gene from 4 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LAMB41   -- laminin, beta 4 83.29(n)
    77.57(a)
    483260  XM_540379.2  XP_540379.2 
    chimpanzee
    (Pan troglodytes)
    LAMB41   -- laminin, beta 4 98.8(n)
    98.11(a)
    463657  XM_519311.2  XP_519311.2 
    chicken
    (Gallus gallus)
    LAMB41   -- laminin, beta 4 65.7(n)
    59.45(a)
    396076  XM_001232877.1  XP_001232878.1 
    zebrafish
    (Danio rerio)
    AF468050.12   --   -- 76.08(n) 286831  AF468050.1 
    About this table        Species with no ortholog for LAMB4

    ENSEMBL Gene Tree for LAMB4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for LAMB4 gene
    LAMB12  LAMB22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/604 NCBI SNPs in LAMB4 are shown (see all 604 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 213)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 7 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs16141881,2
    A,C,F,H,O107451445(+) TGGAAA/CCTGTT 1 -- ut3117Minor allele frequency- C:0.11EA NS NA EU WA 2540
    rs16273541,2
    A,C,F,H,O107465220(+) TAGGTG/AAATGT 1 Y/H mis1 ese313Minor allele frequency- A:0.20MN NA EU EA WA 1234
    rs96906881,2
    A,C,F,H107507398(+) TGTAAC/AAGGGT 1 F/V mis1 ese39Minor allele frequency- A:0.16EU EA WA NA 910
    rs17321521,2
    A,C,F,H107451183(+) AGAATT/CCCCTG 1 -- ng514Minor allele frequency- C:0.20EU EA WA 410
    rs17321511,2
    A,C,F,H107450977(+) TCATAC/TGTGCA 1 -- ng514Minor allele frequency- T:0.21EU EA WA 418
    rs22404451,2
    C,F,H107494131(+) ACCTAT/CTACAA 1 S/N mis116Minor allele frequency- C:0.11EA NA EU WA 2850
    rs104878401,2
    C,F,H107558524(+) TTAAAT/CGGCTC 1 -- ng3112Minor allele frequency- C:0.07NS EU EA WA NA 668
    rs20747491,2
    C,F,H107533668(+) GGTGTG/AGAGCT 1 Y/H mis1 ese313Minor allele frequency- A:0.07EA EU WA 2376
    --
    rs69698751,2
    C,F,H107558811(+) AAGAAT/AAATGA 1 -- ng31 trp38Minor allele frequency- A:0.03EU EA WA 840
    rs25286931,2
    C,F,H107458643(+) ATTTCC/GCTGGT 1 R/S mis111Minor allele frequency- G:0.10EU EA WA NA 976
    About this table

    HapMap Linkage Disequilibrium images for LAMB4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    Human Genome Epidemiology Navigator: LAMB4 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for LAMB4 gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 22798 HGNC: 6491 AceView: LAMB4 Ensembl:ENSG00000091128 euGenes: HUgn22798
    ECgene: LAMB4 H-InvDB: LAMB4
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for LAMB4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for LAMB4-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for LAMB4 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for LAMB4 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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