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LAG3 Gene

protein-coding   GIFtS: 60

GC12P006751
lymphocyte-activation gene 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CD223 1, 2
FDC 3
LAG-3 3
Descriptions
CD223 antigen 3
FDC protein 3
lymphocyte-activation gene 3 2
lymphocyte-activation protein 3 2
External Ids
HGNC: 64761
Entrez Gene: 39022
UniProtKB: P186273
Ensembl: ENSG000000896927
Search outside databases for aliases for LAG3 gene

Previous GC identifers: GC12M006669 GC12M006794 GC12P006760

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for LAG3:
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like
domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and
chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq]

UniProtKB/Swiss-Prot: LAG3_HUMAN, P18627
Function: Involved in lymphocyte activation. Binds to HLA class-II antigens

Gene Wiki entry for LAG3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the LAG3 gene  

Entrez Gene cytogenetic band: 12p13.32   Ensembl cytogenetic band:  12p13.31   HGNC cytogenetic band: 12p13.3

LAG3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P006751:     (about GC identifiers)

Start:
6,751,931 bp from pter
End:
6,757,880 bp from pter
Size:
5,950 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_009759.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: LAG3_HUMAN, P18627 (See protein sequence)
Recommended Name: Lymphocyte activation gene 3 protein precursor  
Size: 525 amino acids; 57437 Da
Subcellular location: Membrane; Single-pass type I membrane protein

REFSEQ proteins: NP_002277.4  

ENSEMBL proteins: 
ENSP00000203629 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
R&D Systems Recombinant & Natural Proteins for LAG3 (LAG-3)
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Lymphocyte Activation Gene 3)
Human Recombinant Proteins from Abnova (LAG3)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0009897 external side of plasma membrane IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for LAG3: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for LAG3
R&D Systems Antibodies for LAG3 (LAG-3)
Antibodies from Abcam (Lymphocyte Activation Gene 3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (LAG3)
Novus Biologicals Antibody for LAG3

Assays for LAG3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
R&D Systems ELISAs for LAG3 (LAG-3)         (see all)
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/6 InterPro domains/families (see all 6 ):
 IPR015621 IL1-receptor
 IPR007110 Ig-like
 IPR013783 Ig-like_fold
 IPR003599 Ig_sub
 IPR013106 Ig_V-set


   GeneDecks  LAG3 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P18627

ProtoNet protein and cluster: P18627

1 Blocks protein family: IPB003599 Immunoglobulin subtype

UniProtKB/Swiss-Prot: LAG3_HUMAN, P18627
Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (LAG3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002286

              Applied Biosystems Silencer® siRNAs for LAG3

              Sigma-Aldrich siRNA and siRNA Panels for LAG3  
                     Sigma-Aldrich shRNA Panels and shRNA for LAG3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for LAG3
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002286
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002286
                                 untagged cDNA clone in CMV expression vector: NM_002286 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_002286

UniProtKB/Swiss-Prot: LAG3_HUMAN, P18627
Function: Involved in lymphocyte activation. Binds to HLA class-II antigens

Genatlas biochemistry entry for LAG3:
lymphocyte activation gene-3,with three transcriptional initiation sites,highly homolog to
CD4,expressed exclusively in activated T and NK lymphocytes,major histocompatibility complex (MHC)
class 2 ligand potentially involved in the regulation of immune response

2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Lag3):

hematopoietic systemimmune system

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003823 antigen binding TAS1692078
GO:0004888 transmembrane receptor activity IEA--
GO:0042289 MHC class II protein binding IDA9159144
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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 Gene Network CentralTM Interacting Genes and Proteins Network for  LAG3 


5/8 Interacting proteins for LAG3 (ENSP000002036293) via UniProtKB, MINT, and/or STRING (see all 8 )
InteractantInteraction Details
GeneCardExternal ID(s)
CD4ENSP000000116533STRING (score=.967)
IGF1ENSP000003026653STRING (score=.84)
CD8AENSP000002836353STRING (score=.811)
IFNGENSP000002291353STRING (score=.75)
STMN1ENSP000003505313STRING (score=.748)
About this table

4 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007166 cell surface receptor linked signal transduction IEA--
GO:0045085 negative regulation of interleukin-2 biosynthetic process IEA--
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity IEA--
GO:0050868 negative regulation of T cell activation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for LAG3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (LAG3)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002286

              Sigma-Aldrich siRNA and siRNA Panels for LAG3  
                     Sigma-Aldrich shRNA Panels and shRNA for LAG3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_002286  

REFSEQ mRNAs for LAG3 gene: 

NM_002286.5   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_002286  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002286
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002286
                                 untagged cDNA clone in CMV expression vector: NM_002286 

Additional cDNA sequence: 

AK292104.1 BC037962.1 BC052589.1 X51985.3 

5 DOTS entries:

DT.405739  DT.99963620  DT.70100595  DT.95360560  DT.97791328 

4 AceView cDNA sequences:

BC037962 BC052589 NM_002286 X51985 

highest scoring ESTs for LAG3:

X51985 AW978655 BC037962 AA687676 AA991723 AI263860 AI264375 AI274900 AI949108 AW003135 

Unigene Cluster for LAG3:

Lymphocyte-activation gene 3
Hs.409523  [show with all ESTs]
Unigene Representative Sequence: BC037962


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000203629  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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LAG3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for LAG3

1 / 2 / 3

4 probe-sets matching LAG3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
36776_at2, 3 U95-A 1 1.00 1.00 0.96 0.67 X51985 1.00 1.00 1.00 1
74689_at2, 3 U95-D 1 0.81 1.00 0.92 1.36 AW003135 0.60 1.00 0.82 1

206486_at2, 3 U133-A 1 0.82 1.00 -- -- NM_002286 0.60 1.00 0.82 1

206486_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
GeneDecks  LAG3 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TATAACCTCA

SOURCE GeneReport for Unigene cluster: Hs.409523

Expression variation in blood from EXPOLDB for LAG3

UniProtKB/Swiss-Prot: LAG3_HUMAN, P18627
Tissue specificity: On cell surface of activated NK and T-lymphocytes

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for LAG3 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
LAG31   -- lymphocyte-activation gene 3 99.05(n)
98.86(a)
451794  XM_508966.2  XP_508966.2 
cow
(Bos taurus)
LAG31   -- lymphocyte-activation gene 3 80.96(n)
73.65(a)
515155  XM_593126.3  XP_593126.3 
rat
(Rattus norvegicus)
Lag31   -- lymphocyte-activation gene 3 78.63(n)
69.22(a)
297596  NM_212513.2  NP_997678.2 
mouse
(Mus musculus)
Lag31, 5 65
lymphocyte-activation gene 31, 5 79.5(n)1
70.21(a)1
167681  NM_008479.11  NP_032505.11 
 BC1205915  CO0402355  (see all 6)
chicken
(Gallus gallus)
LAG31   -- lymphocyte-activation gene 3 47.53(n)
36.99(a)
418287  XM_416510.2  XP_416510.2 
About this table        Species with no ortholog for LAG3

ENSEMBL Gene Tree for LAG3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/35 NCBI SNPs in LAG3 are shown (see all 35 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 37)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 12 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs8708491,2
A,C,F,H6757281(+) TCTCAC/TCCTTG 1 I/T mis19Minor allele frequency- T:0.43MN EU EA WA 1022
rs23013331,2
C,F,H6751257(+) TTCACG/ACTCCC 1 -- ng51 tfbs34Minor allele frequency- A:0.04EU EA WA 418
--
rs73423331,2
C6752096(+) ATTCCA/GGCCTC 1 -- ut51 ese30--------
rs110643771,2
A,C,F,H6757141(+) attgcA/Gtctgt 1 -- int14Minor allele frequency- G:0.05EU EA WA 420
rs18825451,2
A,C,F,H6755671(-) TATAAC/TATCTA 1 -- int110Minor allele frequency- T:0.40NS EU EA WA 646
rs23023651,2
A,C,F,H6754505(+) TTGCCG/AGCCTT 1 -- int15Minor allele frequency- A:0.32EA EU WA 1900
rs23650941,2
A,C,F,H6753931(+) CAGGTG/CCCTGT 1 -- int14Minor allele frequency- C:0.29EU EA WA 418
rs37827351,2
A,C,F,H6755337(+) GGTTAA/GAGTTG 1 -- int114Minor allele frequency- G:0.42EA NS EU WA NA 2354
rs8692221,2
A,F6753951(-) CAAGCC/TGGAAG 1 -- int13Minor allele frequency- T:0.10NA EA 232
rs47646201,2
A,C6755490(-) ATGGCC/TGATAG 1 -- int10--------
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HapMap Linkage Disequilibrium images for LAG3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 153337

4 Novoseek disease relationships for LAG3 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
carcinoma renal cell 9.19 1 11527700 (1)
immunodeficiency 7.32 1 1380059 (1)
necrosis 0.00 1 15969678 (1)
tumors 0.00 9 10601994 (3), 18519788 (2), 19454660 (1), 15969678 (1)
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Genetic Association Database: LAG3
Human Genome Epidemiology Navigator: LAG3 (3 documents)

(Possibly Related Articles in Doctor's Guide)
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  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/64 PubMed articles for LAG3 gene (see all 64 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3902 HGNC: 6476 AceView: LAG3 Ensembl:ENSG00000089692 euGenes: HUgn3902
ECgene: LAG3 H-InvDB: LAG3
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for LAG3 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for LAG3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for LAG3 Antibodies for LAG3
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for LAG3 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
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 Search Tocris compounds for LAG3




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 Antibodies for LAG3

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