KRT74 Gene
protein-coding GIFtS: 49
GCID: GC12M052959
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|
keratin 74
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Aliases
for KRT74 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Keratin 741 2 | | Cytokeratin-743 | | K6IRS41 2 3 5 | | Keratin 5c2 | | KRT6IRS41 2 3 5 | | Keratin 6 Irs42 | | KRT5C1 2 3 | | Keratin, Type II Cytoskeletal 742 | | Type II Inner Root Sheath-Specific Keratin-K6irs42 3 | | Keratin-5c3 | | Type-II Keratin Kb372 3 | | Keratin-743 | | CK-742 3 | | Cytokeratin-743 | | K5C2 3 | | KB373 | | K742 3 | | Keratin-5c3 | | HTSS22 | | Keratin-743 |
Export aliases for KRT74 gene to outside databasesPrevious GC identifers: GC12M051246 GC12M050003 |
Summaries
for KRT74 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for KRT74:
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and aresubdivided into epithelial keratins and hair keratins. This protein belongs to a family of keratins that arespecifically expressed in the inner root sheath of hair follicles.(provided by RefSeq, Jun 2009)
UniProtKB/Swiss-Prot: K2C74_HUMAN, Q7RTS7Function: Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath(IRS) of the hair follicle (Probable)
|
Genomic Views
for KRT74 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000012.11 NC_018923.1 NT_029419.12
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the KRT74 gene promoter:
ER-alpha FOXF2 AML1a p53 LCR-F1 E47 GATA-1 POU2F1 POU2F1a
Other transcription factors
Search SABiosciences Chromatin IP Primers for KRT74
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat KRT74 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 12q13.13 Ensembl cytogenetic band: 12q13.13 HGNC cytogenetic band: 12q13.13KRT74 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 12 GeneLoc Exon Structure GeneLoc location for GC12M052959: view genomic region
(about GC identifiers)
Start:
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52,959,566 bp from pter |
End:
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52,967,609 bp from pter |
Size:
|
8,044 bases |
Orientation:
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minus strand |
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Proteins
for KRT74 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: K2C74_HUMAN, Q7RTS7 (See
protein sequence)Recommended Name: Keratin, type II cytoskeletal 74 Size: 529 amino acids; 57865 Da
Subunit: Heterotetramer of two type I and two type II keratins
Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic)(40-55 and 56-70 kDa, respectively)
Sequence caution: Sequence=DAA00404.1; Type=Erroneous gene model prediction;
Secondary accessions: B5MD61 Q86Y45Explore the universe of human proteins at neXtProt for KRT74: NX_Q7RTS7
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q7RTS7
KRT74 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_778223.2
ENSEMBL proteins: ENSP00000447447 ENSP00000307240
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0045095 | keratin filament |
IEA | -- |
KRT74 for ontologies About GeneDecksing
KRT74 Antibody Products:
Assay Products for KRT74: |
Protein
Domains /
Families
for KRT74 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
KRT74 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q7RTS7ProtoNet protein and cluster: Q7RTS7 2 Blocks protein families: IPB001664 Intermediate filament protein IPB003054 Type II keratin signature
UniProtKB/Swiss-Prot: K2C74_HUMAN, Q7RTS7Similarity: Belongs to the intermediate filament family |
Function
for KRT74 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: K2C74_HUMAN, Q7RTS7Function: Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath(IRS) of the hair follicle (Probable)
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for KRT74
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for KRT74
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for KRT74 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005198 | structural molecule activity |
IEA | -- |
KRT74 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for KRT74:
|
Pathways & Interactions
for KRT74 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for KRT74
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
2 Interacting proteins for KRT74 (Q7RTS73 ENSP000003072404) via UniProtKB, MINT, STRING, and/or I2DAbout this table
|
Drugs & Compounds
for KRT74 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for KRT74
Search CenterWatch for drugs/clinical trials and news about KRT74 / K2C74
|
Transcripts
for KRT74 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for KRT74 gene: NM_175053.3 Unigene Cluster for KRT74: Keratin 74 Hs.660125 [show with all ESTs]Unigene Representative Sequence: AJ5087773 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000549343 ENST00000305620(uc001sap.1) ENST00000546384
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for KRT74
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for KRT74
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: KRT74 (NM_175053) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for KRT74 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat KRT74 |
Additional cDNA sequence: AF086480.1 AJ508777.1 1 DOTS entry: DT.111133 3 AceView cDNA sequences: NM_175053 AF086480 AJ508777
GeneLoc Exon Structure
|
Expression
for KRT74 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| KRT74 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See KRT74 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for KRT74
SOURCE GeneReport for Unigene cluster: Hs.660125
UniProtKB/Swiss-Prot: K2C74_HUMAN, Q7RTS7Tissue specificity: Highly expressed in hair follicles from scalp. In hair, it is specifically present in the innerroot sheath (IRS) of the hair follicle. Present in the IRS Huxley layer, but not in Henle layer or cuticle of the IRS.In the IRS Huxley layer, it is expressed in specialized Huxley cells, termed 'Fluegelzellen, along the area ofdifferentiated Henle cells (at protein level)
SABiosciences Custom PCR Arrays for KRT74
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for KRT74
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat KRT74 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat KRT74 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat KRT74 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for KRT74 |
Orthologs
for KRT74 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for KRT74 gene from 4/14 species (see all 14) About this table
ENSEMBL Gene Tree for KRT74 (if available)
TreeFam Gene Tree for KRT74 (if available) |
Paralogs
for KRT74 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for KRT74 gene
- KRT772 KRT6B2 KRT832 KRT762 KRT862 KRT32 KRT842 KRT82
- KRT812 KRT712 KRT8P112 KRT782 KRT72 ENSG000001704422 KRT792 KRT722
- KRT732 KRT52 KRT6C2 KRT6A2 KRT852 KRT22 KRT752 KRT12
- KRT822 KRT42
18/39 SIMAP similar genes for KRT74 using alignment to 2 protein entries: K2C74_HUMAN (see all proteins)
(see all similar genes):KRTHB6 KRT71 KRT79 KRT5 KRT72 KRT1B
KRT4 KRT6A KRT7 KRT6B KRT6C KRT78
KRT75 KRT77 KRT73 KRT121P KRT86 KRT1
KRT74 for paralogs About GeneDecksing
|
Genomic Variants
for KRT74 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 12 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for KRT74 (52959566 - 52967609 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for KRT74
1 Indel: 66224
Human Gene Mutation Database (HGMD): KRT74
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing KRT74 |
|
Disorders
/ Diseases
for KRT74 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
KRT74 for disorders About GeneDecksing
OMIM gene information: 608248
OMIM disorders: 194300
UniProtKB/Swiss-Prot: K2C74_HUMAN, Q7RTS7
Defects in KRT74 are a cause of woolly hair autosomal dominant (ADWH) [MIM:194300]. A hair shaft disordercharacterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations,woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows somestructural anomalies, including trichorrhexis nodosa and tapered ends Defects in KRT74 are the cause of hypotrichosis type 3 (HYPT3) [MIM:613981]. A condition characterized by thepresence of less than the normal amount of hair. Affected individuals have normal hair in early childhood butexperience progressive hair loss limited to the scalp beginning in the middle of the first decade and almost completebaldness by the third decade. Body hair, beard, eyebrows, axillary hair, teeth, and nails develop normally
4  diseases for KRT74: About MalaCardswoolly hair, autosomal dominant hypotrichosis pharyngitis neuronitis
1 disease from the University of Copenhagen DISEASES database for KRT74:Hypotrichosis Human Genome Epidemiology (HuGE) Navigator: KRT74 (3 documents)
Export disorders for KRT74 gene to outside databases
|
Publications
for KRT74 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for KRT74 gene, integrated from 9 sources (see all 13):
(articles sorted by number of sources associating them with KRT74) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- K6irs1, K6irs2, K6irs3, and K6irs4 represent the inner-root-sheath-specific type II epithelial keratins of the human hair follicle. (PubMed id 12648212)1, 2, 3, 9 Langbein L....Schweizer J. (2003)
- Novel mutations in the keratin-74 (KRT74) gene underl ie autosomal dominant woolly hair/hypotrichosis in Pakistani families. (PubMed id 21188418)1, 2 Wasif N....Ahmad W. (2011)
- Autosomal-dominant woolly hair resulting from disrupt ion of keratin 74 (KRT74), a potential determinant of human hair texture. (PubMed id 20346438)1, 2 Shimomura Y....Christiano A.M. (2010)
- New consensus nomenclature for mammalian keratins. (PubMed id 16831889)1, 3 Schweizer J....Wright M.W. (2006)
- Genes for intermediate filament proteins and the draft sequence of the human genome: novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18. (PubMed id 11683385)1, 2 Hesse M.... Weber K. (2001)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
- Gene variants associated with ischemic stroke: the cardiovascular health study. (PubMed id 19023099)1 Luke M.M....Psaty B.M. (2009)
- Association of gene variants with incident myocardial infarction in the Cardiovascular Health Study. (PubMed id 17975119)1 Shiffman D....Psaty B.M. (2008)
|
External Searches for KRT74 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing KRT74 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing KRT74 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing KRT74 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for KRT74 | Pharmacogenomics, SNPs, Pathways | | Human Intermediate Filament Mutation Database | http://www.interfil.org |
|
| | |
About This Section
| Patent Information for KRT74 gene:
Search GeneIP for patents involving KRT74
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for KRT74 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for KRT74 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for KRT74 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for KRT74 | | | OriGene Protein Over-expression Lysate for KRT74 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for KRT74 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for KRT74 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for KRT74 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat KRT74 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing KRT74 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat KRT74 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat KRT74 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat KRT74 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat KRT74 |
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| |  |  |  |  | | | | |
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 | | | KRT74 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for KRT74 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat KRT74 |
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