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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

KLHL21 Gene

protein-coding   GIFtS: 49
GCID: GC01M006586

kelch-like family member 21

(Previous name: kelch-like 21 (Drosophila) )
  Search for KLHL21
in our new
 Human Malady Compendium 
Biological research products
for KLHL21
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Kelch-Like Family Member 211 2
KIAA04691 3
Kelch-Like 21 (Drosophila)1
Kelch-Like 212
Kelch-Like Protein 212

External Ids:    HGNC: 290411   Entrez Gene: 99032   Ensembl: ENSG000001624137   UniProtKB: Q9UJP43   

Export aliases for KLHL21 gene to outside databases

Previous GC identifer: GC01M005798


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: KLH21_HUMAN, Q9UJP4
Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for
efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of
the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the
ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its
degradation by the proteasome




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_021937.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the KLHL21 gene promoter:
         Bach1   Lmo2   MIF-1   NF-E2 p45   FOXL1   CHOP-10   PPAR-gamma1   PPAR-gamma2   NF-E2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidKLHL21 promoter sequence
   Search SABiosciences Chromatin IP Primers for KLHL21

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat KLHL21


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p36.31   Ensembl cytogenetic band:  1p36.31   HGNC cytogenetic band: 1p36

KLHL21 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
KLHL21 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M006586:  view genomic region     (about GC identifiers)

Start:
6,650,784 bp from pter      End:
6,674,667 bp from pter
Size:
23,884 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: KLH21_HUMAN, Q9UJP4 (See protein sequence)
Recommended Name: Kelch-like protein 21  
Size: 597 amino acids; 66617 Da
Subunit: Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1
Subcellular location: Cytoplasm, cytoskeleton, spindle. Note=Localizes to the spindle midzone and targets CUL3 to this
region
Sequence caution: Sequence=BAA32314.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: B3KQP2 O75057 Q5SY26 Q5SY28 Q8N4I6 Q8NF10
Alternative splicing: 2 isoforms:  Q9UJP4-1   Q9UJP4-2   (Ref.1 (BAA32314) sequence is in conflict in position: 534:G->D)

Explore the universe of human proteins at neXtProt for KLHL21: NX_Q9UJP4

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9UJP4

  • KLHL21 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_055666.2  
    ENSEMBL proteins: 
     ENSP00000366886   ENSP00000366891   ENSP00000466089   ENSP00000468710   ENSP00000466479  
    Reactome Protein details: Q9UJP4
    Human Recombinant Protein Products: 
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    Uscn Proteins for KLHL21

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--
    GO:0005827polar microtubule IDA19995937
    GO:0031463Cul3-RING ubiquitin ligase complex IDA19995937


    KLHL21 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    KLHL21 for domains           About GeneDecksing

    5/7 InterPro domains/families (see all 7):
     IPR017096 Kelch-like_gigaxonin
     IPR000210 BTB/POZ-like
     IPR006652 Kelch_1
     IPR011333 BTB/POZ_fold
     IPR015916 Gal_Oxidase_b-propeller

    Graphical View of Domain Structure for InterPro Entry Q9UJP4

    ProtoNet protein and cluster: Q9UJP4

    2 Blocks protein families:
    IPB000210 BTB/POZ domain
    IPB011705 BTB/Kelch-associated


    UniProtKB/Swiss-Prot: KLH21_HUMAN, Q9UJP4
    Similarity: Contains 1 BACK (BTB/Kelch associated) domain
    Similarity: Contains 1 BTB (POZ) domain
    Similarity: Contains 6 Kelch repeats


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: KLH21_HUMAN, Q9UJP4
    Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for
    efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of
    the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the
    ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its
    degradation by the proteasome

    miRNA
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    hsa-miR-3130-3p hsa-miR-3155 hsa-miR-548aa hsa-miR-3921 hsa-miR-484 hsa-miR-146a* hsa-miR-593 hsa-miR-4310
    SwitchGear 3'UTR luciferase reporter plasmidKLHL21 3' UTR sequence
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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842contributes to ubiquitin-protein ligase activity IDA19995937


    KLHL21 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Antigen processing: Ubiquitination & Proteasome degradation
    Antigen processing: Ubiquitination & Proteasome degradation1.00
    Polyubiquitination of substrate0.75
    Class I MHC mediated antigen processing & presentation0.83
    2Immune System
    Immune System1.00
    Adaptive Immune System0.59

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    5        Reactome Pathways for KLHL21
        Antigen processing: Ubiquitination & Proteasome degradation
    Polyubiquitination of substrate
    Adaptive Immune System
    Class I MHC mediated antigen processing & presentation
    Immune System


    UniProtKB/Swiss-Prot: KLH21_HUMAN, Q9UJP4
    Pathway: Protein modification; protein ubiquitination


    KLHL21 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for KLHL21

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/53 Interacting proteins for KLHL21 (Q9UJP43 ENSP000003668864) via UniProtKB, MINT, STRING, and/or I2D (see all 53)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    AURKBQ96GD43, ENSP000003139504I2D: score=1 STRING: ENSP00000313950
    CUL3Q136183, ENSP000002644144I2D: score=1 STRING: ENSP00000264414
    KLHL13Q9P2N73, ENSP000002628204I2D: score=1 STRING: ENSP00000262820
    KLHL9Q9P2J33, ENSP000003519334I2D: score=1 STRING: ENSP00000351933
    BIRC5ENSP000003016334STRING: ENSP00000301633
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000910cytokinesis IMP19995937
    GO:0007067mitosis IEA--
    GO:0016567protein ubiquitination IDA19995937
    GO:0051322anaphase IMP19995937


    KLHL21 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for KLHL21
    Search CenterWatch for drugs/clinical trials and news about KLHL21 / KLH21 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for KLHL21 gene: 
    NM_014851.2  

    Unigene Cluster for KLHL21:

    Kelch-like 21 (Drosophila)
    Hs.7764  [show with all ESTs]
    Unigene Representative Sequence: NM_014851
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000377658(uc009vme.3 uc001aoa.3) ENST00000377663(uc001anz.1)
    ENST00000467612 ENST00000496707 ENST00000463043

    miRNA
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    hsa-miR-3130-3p hsa-miR-3155 hsa-miR-548aa hsa-miR-3921 hsa-miR-484 hsa-miR-146a* hsa-miR-593 hsa-miR-4310
    SwitchGear 3'UTR luciferase reporter plasmidKLHL21 3' UTR sequence
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    Additional cDNA sequence: 

    AK057164.1 AK075305.1 AK090472.1 AK307564.1 BC034039.1 BC064980.1 BC091648.1 BC111766.1 

    12 DOTS entries:

    DT.100790856  DT.95279151  DT.414837  DT.100790853  DT.92442673  DT.100790852  DT.121348553  DT.121348589 
    DT.70100058  DT.87013624  DT.91833629  DT.95153309 

    1 AceView cDNA sequence:

    AL079660 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    KLHL21 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TCCAGAATCC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See KLHL21 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for KLHL21

    SOURCE GeneReport for Unigene cluster: Hs.7764
        SABiosciences Expression via Pathway-Focused PCR Array including KLHL21: 
              Ubiquitin Ligases in human mouse rat

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for KLHL21 gene from 3/17 species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves KLHL211 kelch-like 21 (Drosophila) 79.52(n)
    82.04(a)
      428175  XM_003642517.1  XP_003642565.1 
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.82692 Transcribed sequence with weak similarity to protein spQ9UJP4 (H.sapiens) Y469_HUMAN Hypothetical protein KIAA0469 less 76.28(n)    CF224081.1 
    zebrafish
    (Danio rerio)
    Actinopterygii klhl211 kelch-like 21 (Drosophila) 67.91(n)
    73.13(a)
      404613  NM_207081.1  NP_996964.1 


    ENSEMBL Gene Tree for KLHL21 (if available)
    TreeFam Gene Tree for KLHL21 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for KLHL21 gene
    KLHL382  KLHL292  KLHL62  KLHL232  KBTBD32  KLHL112  KLHL242  ENC12  
    KLHL252  KLHL302  KLHL352  CCIN2  
    18/23 SIMAP similar genes for KLHL21 using alignment to 6 protein entries:     KLH21_HUMAN (see all proteins) (see all similar genes):
    KLHL17    IVNS1ABP    KLHL20    KBTBD3    KLHL24    KLHL29
    KLHL32    BACH2    KLHL30    KLHL3    KLHL2    KLHL6
    BTBD3    KLHL18    KLHL25    KLHL38    ENC1    KLHL5

    KLHL21 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/267 NCBI SNPs in KLHL21 are shown (see all 267    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1379922921,2
    Cother6653500(+) AACTCG/AAAGCC 2 /F syn11Minor allele frequency- A:0.00NA 4546
    rs1162899531,2
    F,--6650316(+) TCATCG/CCAGAT 1 -- int11Minor allele frequency- C:0.03WA 118
    rs1915855681,2
    --6650363(+) AAGAAA/GGGCTG 1 -- int10--------
    rs1153039941,2
    F,--6650377(+) GAGCCG/AGGCCC 1 -- int11Minor allele frequency- A:0.02WA 118
    rs1825100991,2
    --6650380(+) CCGGGC/TCCGGG 1 -- int10--------
    rs1851756001,2
    --6650431(+) GTTTCC/GTGGGC 1 -- int10--------
    rs1907694371,2
    --6650441(+) CATCAG/TCTGTT 1 -- int10--------
    rs1833260051,2
    --6650467(+) CTCAGA/GCCTCT 1 -- int10--------
    rs786556811,2
    C,--6650476(+) TCTCT-/TTAAATG 1 -- int10--------
    rs2002847171,2
    C--6650477(+) TCTCTC/TTAAAT 1 -- int10--------

    HapMap Linkage Disequilibrium report for KLHL21 (6650784 - 6674667 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for KLHL21
         1 CNV: 4201

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for KLHL21 gene, integrated from 9 sources (see all 13):
    (articles sorted by number of sources associating them with KLHL21)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis. (PubMed id 19995937)1, 2 Maerki S....Peter M. (2009)
    2. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
    3. Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PubMed id 16303743)1, 2 Otsuki T....Isogai T. (2005)
    4. Characterization of cDNA clones in size-fractionated cDNA libraries from human brain. (PubMed id 9455484)1, 2 Seki N.... Ohara O. (1997)
    5. Proteome-wide identification of ubiquitylation sites b y conjugation of engineered lysine-less ubiquitin. (PubMed id 22053931)1 Oshikawa K....Nakayama K.I. (2012)
    6. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    7. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
    8. Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)1 Lee K.A....Doedens J.R. (2011)
    9. Dynamics of cullin-RING ubiquitin ligase network revea led by systematic quantitative proteomics. (PubMed id 21145461)1 Bennett E.J....Harper J.W. (2010)
    10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 9903 HGNC: 29041 AceView: KIAA0469.1 Ensembl:ENSG00000162413 euGenes: HUgn9903
    ECgene: KLHL21 H-InvDB: KLHL21

    (According to HUGE)
    About This Section
    HUGE: KIAA0469

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for KLHL21 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for KLHL21 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for KLHL21 gene:
    Search GeneIP for patents involving KLHL21

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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