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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

KLF4 Gene

protein-coding GIFtS: 64
GCID: GC09M110247

Kruppel-like factor 4 (gut)

Explore 42 diseases affiliated with
KLF4 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Kruppel-Like Factor 4 (Gut)¹ ²		Gut-Enriched Krueppel-Like Factor² ³
EZF¹ ² ³ ⁵		Endothelial Kruppel-Like Zinc Finger Protein²
GKLF¹ ² ³ ⁵		Krueppel-Like Factor 4²
Epithelial Zinc Finger Protein EZF² ³

External Ids:	HGNC: 6348¹	Entrez Gene: 9314²	Ensembl: ENSG00000136826⁷	OMIM: 602253⁵	UniProtKB: O43474³

Export aliases for KLF4 gene to outside databases

Previous GC identifers: GC09M101042 GC09M101979 GC09M103704 GC09M105626 GC09M107326 GC09M109286 GC09M079848

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: KLF4_HUMAN, O43474

Function: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds

to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key

transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and

in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal

maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also

function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription

Gene Wiki entry for KLF4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000009.11 NC_018920.1 NT_008470.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the KLF4 gene promoter:
SRF STAT1 AP-2alpha isoform 3 SRF (504 AA) AP-2alpha isoform 4 AP-2alpha isoform 2 AP-2alpha AP-2alphaA
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): KLF4 promoter sequence

Search SABiosciences Chromatin IP Primers for KLF4

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat KLF4

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q31 Ensembl cytogenetic band: 9q31.2 HGNC cytogenetic band: 9q31

KLF4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
KLF4 gene location

GeneLoc information about chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09M110247: view genomic region (about GC identifiers)

Start:	110,247,133 bp from pter	End:	110,252,763 bp from pter
Size:	5,631 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: KLF4_HUMAN, O43474 (See protein sequence)

Recommended Name: Krueppel-like factor 4

Size: 513 amino acids; 54671 Da

Subunit: Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain).

Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a

Subcellular location: Nucleus

Biotechnology: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are

sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem)

cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes

Sequence caution: Sequence=AAB48399.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Sequence=AAC03462.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAD42165.1;

Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH29923.1; Type=Erroneous initiation;

Note=Translation N-terminally extended; Sequence=AAH30811.1; Type=Erroneous initiation; Note=Translation N-terminally

extended; Sequence=ABG25917.1; Type=Erroneous gene model prediction; Sequence=BAG36271.1; Type=Erroneous initiation;

Note=Translation N-terminally extended; Sequence=EAW59020.1; Type=Erroneous gene model prediction;

Sequence=EAW59021.1; Type=Erroneous gene model prediction;

Secondary accessions: B2R8S4 B3KT79 P78338 Q5T3J8 Q5T3J9 Q8N717 Q9UNP3

Alternative splicing: 3 isoforms: O43474-3 O43474-1 O43474-4 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for KLF4: NX_O43474

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_O43474

KLF4 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_004226.3

ENSEMBL proteins:

ENSP00000363804 ENSP00000399921 ENSP00000396294 ENSP00000404922

Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: KLF4 OriGene Protein Over-expression Lysate (see all 2): KLF4 OriGene Custom Protein Services for KLF4
	GenScript Custom Purified and Recombinant Proteins Services for KLF4
	Novus Biologicals KLF4 Proteins Novus Biologicals KLF4 Lysates
	Browse Sino Biological Recombinant Proteins
	ProSpec Recombinant Protein for KLF4
	Uscn Proteins for KLF4

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000785	chromatin	--	--
GO:0000790	nuclear chromatin	IDA	--
GO:0005634	nucleus	--	--
GO:0005667	transcription factor complex	IEA	--

KLF4 for ontologies About GeneDecksing

KLF4 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of KLF4
	R&D Systems Antibodies for KLF4
	Cell Signaling Technology (CST) Antibodies for KLF4
	OriGene Antibodies (see all 2): KLF4 OriGene Custom Antibody Services for KLF4
	GenScript Custom Superior Antibodies Services for KLF4
	Novus Biologicals KLF4 Antibodies
	Abcam antibodies for KLF4
	Uscn Antibodies for KLF4
	ThermoFisher Antibody for KLF4

Assay Products for KLF4:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems Transcription Factor Binding & Immunoprecipitation Assays for KLF4
	GenScript Custom Assay Services for KLF4
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for KLF4

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

KLF4 for domains About GeneDecksing

3 InterPro domains/families:

IPR015880 Znf_C2H2-like

IPR013087 Znf_C2H2/integrase_DNA-bd

IPR007087 Znf_C2H2

Graphical View of Domain Structure for InterPro Entry O43474

ProtoNet protein and cluster: O43474

1 Blocks protein family: IPB007086 C2H2-type zinc finger signature

UniProtKB/Swiss-Prot: KLF4_HUMAN, O43474

Domain: the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription

factors

Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family

Similarity: Contains 3 C2H2-type zinc fingers

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: KLF4_HUMAN, O43474

Function: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds

to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key

transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and

in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal

maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also

function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription

Genatlas biochemistry entry for KLF4:

Kruppel-like factor 4 (gut),zinc finger protein,mouse EZF and GKLF homolog,nuclear,expressed in select

tissues,colon,testis,lung and small intestine,binding DNA fragments,containing the CACCC motif of the beta globin

promoter,acting both as a transcriptional activator and repressor

miRNA Products:		miRTarBase miRNAs that target KLF4: hsa-mir-10b (MIRT003216), hsa-mir-25 (MIRT003605), hsa-mir-145 (MIRT000308), hsa-mir-130a (MIRT005674), hsa-mir-135b (MIRT005440)

		OriGene 3'-UTR Clone: KLF4 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat KLF4
		8/65 QIAGEN miScript miRNA Assays for microRNAs that regulate KLF4 (see all 65):
		hsa-miR-579 hsa-miR-520f hsa-miR-300 hsa-miR-128 hsa-miR-632 hsa-miR-200b hsa-miR-4273 hsa-miR-92b

		SwitchGear 3'UTR luciferase reporter plasmid: KLF4 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for KLF4 (see all 7) OriGene shRNA RFP: KLF4 OriGene siRNA: KLF4
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat KLF4

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for KLF4

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for KLF4 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for KLF4 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: KLF4 (NM_004235)
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat KLF4

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for KLF4
		Search LifeMap BioReagents cell lines for KLF4

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for KLF4

Gene Ontology (GO): 5/14 molecular function terms (GO ID links to tree view) (see all 14): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000987	core promoter proximal region sequence-specific DNA binding	IDA	--
GO:0001010	sequence-specific DNA binding transcription factor recruiting transcription factor activity	ISS	--
GO:0001077	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription	IMP	--
GO:0001085	RNA polymerase II transcription factor binding	ISS	--
GO:0001190	RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription	IDA	--

KLF4 for ontologies About GeneDecksing

1 GenomeRNAi human phenotype for KLF4:

Decreased viability with pacli

Animal Models:
Mouse knock-outs for KLF4: Klf4^tm1.1Khk Klf4^tm1Efu
11 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Klf4):

behavior/neurological	cellular	craniofacial	digestive/alimentary	endocrine/exocrine gland
homeostasis/metabolism	integument	mortality/aging	normal	reproductive system
tumorigenesis

KLF4 for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Selected targets of Oct-3/4

Selected targets of Oct-3/4

1.00

Selected targets of CREB1

Selected targets of CREB1

1.00

SRF and miRs in Smooth Muscle Differentiation and Proliferation

SRF and miRs in Smooth Muscle Differentiation and Proliferation

1.00

Regulation of Wnt-mediated beta catenin signaling and target gene transcription

Regulation of Wnt-mediated beta catenin signaling and target gene transcription

1.00

Wnt / Hedgehog / Notch

Wnt / Hedgehog / Notch

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

2 EMD Millipore Pathways for KLF4

	Selected targets of Oct-3/4
	Selected targets of CREB1

1 Cell Signaling Technology (CST) Pathway for KLF4

Wnt / Hedgehog / Notch

2 BioSystems Pathways for KLF4

	SRF and miRs in Smooth Muscle Differentiation and Proliferation
	Regulation of Wnt-mediated beta catenin signaling and target gene transcription

KLF4 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for KLF4

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/72 Interacting proteins for KLF4 (O43474^{2, 3} ENSP00000363804⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 72)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CTBP1	Q13363²^,³, ENSP00000290921⁴	MINT-7262023 MINT-7261981 MINT-7261995 MINT-7262008 I2D: score=1 STRING: ENSP00000290921
CREBBP	Q92793³, ENSP00000262367⁴	I2D: score=3 STRING: ENSP00000262367
TP53	P04637³, ENSP00000269305⁴	I2D: score=2 STRING: ENSP00000269305
HDAC7	Q8WUI4³, ENSP00000080059⁴	I2D: score=1 STRING: ENSP00000080059
EP300	Q09472³, ENSP00000263253⁴	I2D: score=1 STRING: ENSP00000263253

About this table

Gene Ontology (GO): 5/37 biological process terms (GO ID links to tree view) (see all 37): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000122	negative regulation of transcription from RNA polymerase II promoter	IDA	--
GO:0006351	transcription, DNA-dependent	--	--
GO:0006355	regulation of transcription, DNA-dependent	--	--
GO:0006357	regulation of transcription from RNA polymerase II promoter	--	--
GO:0006366	transcription from RNA polymerase II promoter	ISS	--

KLF4 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

KLF4 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for KLF4
8 Novoseek chemical compound relationships for KLF4 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
zinc	57.2	28	9218496 (2), 9422764 (1), 9443972 (1), 12518228 (1) (see all 19)
butyrate	34.6	14	15051827 (8), 18264726 (1), 19327128 (1), 11005769 (1) (see all 5)
oligonucleotide	23.3	6	9443972 (1), 17017123 (1), 12900388 (1), 19858207 (1)
biotin	18.5	7	16959947 (2), 19056639 (1)
pd 98,059	6.18	1	16077033 (1)
ornithine	2.16	2	15570219 (1), 12297499 (1)
thymidine	0	5	11322789 (1), 11005769 (1)
nitric oxide	0	5	17339326 (1), 20073046 (1), 16169848 (1), 19858207 (1)

Search CenterWatch for drugs/clinical trials and news about KLF4

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for KLF4 gene:

NM_004235.4

Unigene Cluster for KLF4:

Kruppel-like factor 4 (gut)

Hs.376206 [show with all ESTs]

Unigene Representative Sequence: NM_004235

6 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000497048(uc004bdf.2) ENST00000374672(uc004bdg.3 uc004bdh.3)

ENST00000493306(uc022blk.1) ENST00000411706 ENST00000439281 ENST00000420475

miRNA Products:		OriGene 3'-UTR Clone: KLF4 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat KLF4
		8/65 QIAGEN miScript miRNA Assays for microRNAs that regulate KLF4 (see all 65):
		hsa-miR-579 hsa-miR-520f hsa-miR-300 hsa-miR-128 hsa-miR-632 hsa-miR-200b hsa-miR-4273 hsa-miR-92b

		SwitchGear 3'UTR luciferase reporter plasmid: KLF4 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for KLF4 (see all 7) OriGene shRNA RFP: KLF4 OriGene siRNA: KLF4
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat KLF4

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for KLF4 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for KLF4 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: KLF4 (NM_004235)
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		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat KLF4
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat KLF4

Additional cDNA sequence:

AF022184.1 AF105036.1 AK313489.1 BC029923.1 BC030811.1 HF546201.1 HF546202.1 HF546213.1

HM026463.1 U70663.1

11 DOTS entries:

DT.315747 DT.97854059 DT.100029688 DT.100029691 DT.91642375 DT.121168852 DT.91727640 DT.99945043

DT.100751395 DT.121168874 DT.95268849

24/147 AceView cDNA sequences (see all 147):

CD678426 AA236239 AF105036 AA687950 CA405483 AI266211 AW188056 BM823002

AW196645 AL046513 AI572470 BU752888 AW074303 AW515335 BG546222 BM717628

AA298805 BC030811 AI568487 AA620711 AK095134 CR599303 BG681151 AI687397

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

KLF4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TTTAAATAGC

About this image

KLF4 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

10/17 LifeMap In Vivo Development Anatomical Compartments/Cells (see all 17)

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Bone

Zeugopod Epiphyseal End

Chondrocytes

Bone, Cartilage

Limb

Zeugopod

Mesenchymal Condensate Cells

Bone, Cartilage

Heart

Atrioventricular Canal

Atrioventricular Canal Cells

Myocardium

Ovary

Antral Follicle

Mature Granulosa Cells

Ovary

Bone

Rostral Endochondral Facial Bones

Bone

Rostral Skull Membranous Bones

Bone

Bone

Bone

Bone

Cartilage

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 10/13 LifeMap Cells (see all 13)

Name

Category

Induced pluripotent stem cell line 20D17 (Induced Pluripotent Stem Cell)

Early Embryo, Fibroblast

Induced pluripotent stem cell line 492B4 (Induced Pluripotent Stem Cell)

Early Embryo, Fibroblast

Induced pluripotent stem cell line 201B6 (Induced Pluripotent Stem Cell)

Early Embryo, Fibroblast

Induced pluripotent stem cell line hIPS1 (Induced Pluripotent Stem Cell)

Early Embryo, Fibroblast

Induced pluripotent stem cell line hIPS2 (Induced Pluripotent Stem Cell)

Early Embryo, Fibroblast

Induced pluripotent stem cell line 253G1 (Induced Pluripotent Stem Cell)

Early Embryo, Fibroblast

PureStem™ progenitor T42 (Embryonic Progenitor Cell)

PureStem™ mesenchymal progenitor SM30 (Embryonic Progenitor Cell)

Adipose, Bone, Cartilage

PureStem™ mesenchymal progenitor E15 (Embryonic Progenitor Cell)

Adipose, Bone, Cartilage

PureStem™ progenitor E69 (Embryonic Progenitor Cell)

See KLF4 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for KLF4

SOURCE GeneReport for Unigene cluster: Hs.376206

SABiosciences Expression via Pathway-Focused PCR Arrays including KLF4:

Stem Cell Transcription Factors in human mouse rat

Polycomb & Trithorax Target Genes in human mouse rat

Adipogenesis in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for KLF4 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat KLF4
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat KLF4
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat KLF4

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for KLF4

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for KLF4 gene from 5/18 species (see all 18) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	KLF4¹	Kruppel-like factor 4 (gut)	70.67(n) 68.71(a)		770254 XM_001233583.2 XP_001233584.2
lizard (Anolis carolinensis)	Reptilia	KLF2⁶ KLF4⁶ (see all 3)	--	56(a) 55(a) (see all 3)	possible ortholog 1 ↔ 1 (see all 3)	4(109090962-109093568) GL343218.1(2366850-2377131)
zebrafish (Danio rerio)	Actinopterygii	klf4a¹	Kruppel-like factor 4a	58.09(n) 59.84(a)		562155 NM_001113483.1 NP_001106955.1
fruit fly (Drosophila melanogaster)	Insecta	CG9895³	transcription factor	73(a)		59C1 --
worm (Caenorhabditis elegans)	Secernentea	F56F11.3³	zinc finger protein	79(a) (best of 3)		III(2946712-2952243) --

ENSEMBL Gene Tree for KLF4 (if available)
TreeFam Gene Tree for KLF4 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for KLF4 gene

KLF1² KLF11² KLF6² KLF10² KLF5² KLF14² KLF16² KLF2²

KLF15² KLF9² KLF12² KLF3² KLF7² KLF13² KLF8²

18/103 SIMAP similar genes for KLF4 using alignment to 8 protein entries: KLF4_HUMAN (see all proteins) (see all similar genes):

DKFZp686N0199 KLF7 DKFZp313F0536 KLF1 ZFP1 ZFP112

ZNF19 ZNF544 ZNF83 DKFZp686O1631 HKR1 ZNF253

ZNF56 ZNF671 WT1 ZNF78L1 DKFZp686M04222 ZIC1

KLF4 for paralogs About GeneDecksing

1 Pseudogenes.org Pseudogene for KLF4
PGOHUM00000260450

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 9 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs12340310^1,2	F,A,	--	110246636(+)	`GCTGTA/GGGAAG`	1	--	ds500¹		1		NA	120
rs73525712^1,2	C,	--	110246661(+)	`CAGATG/ATGAGG`	1	--	ds500¹		1		WA	2
rs141345855^1,2		--	110246689(+)	`CCACAC/GAAAAC`	1	--	ds500¹		0	--	--	--	--
rs45590840^1,2	C,F,	--	110246695(-)	`AAAAAA/TGTTTT`	1	--	ds500¹		3		NS WA	212
rs200857627^1,2		--	110246696(+)	`AAACT-/TTTTTA TTTTT`	1	--	ds500¹		0	--	--	--	--
rs180963381^1,2		--	110246701(+)	`TTTTTA/GTTTTT`	1	--	ds500¹		0	--	--	--	--
rs184143824^1,2		--	110246906(+)	`TTTTAA/CTCTTG`	1	--	ds500¹		0	--	--	--	--
rs45562333^1,2	C,F,	--	110246983(-)	`AAAAAA/-CTATT`	1	--	ds500¹		2		NS	92
rs189889027^1,2		--	110246991(+)	`TTTTTC/TAATAA`	1	--	ds500¹		0	--	--	--	--
rs12340462^1,2	F,	--	110246995(+)	`TTAATA/TAAAAA`	1	--	ds500¹		1		NA	120

HapMap Linkage Disequilibrium report for KLF4 (110247133 - 110252763 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for KLF4: --

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

KLF4 for disorders           About GeneDecksing

OMIM gene information: 602253    OMIM disorders: --

20/42 diseases for KLF4 (see all 42):    About MalaCards

familial adenomatous polyposis adenomatous polyposis coli skin squamous cell carcinoma squamous cell carcinoma

breast cancer susceptibility polyposis adult t-cell leukemia monocytic leukemia

ulcerative colitis breast cancer hodgkin's lymphoma teratoma

esophageal cancer t-cell leukemia colon cancer colorectal adenoma and carcinoma

gastric cancer adenoma paralysis seminoma

1 disease from the University of Copenhagen DISEASES database for KLF4:

Teratoma

10/23 Novoseek disease relationships for KLF4 gene (see all 23) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
squamous cell esophageal cancer	69.5	6	12439907 (3), 12518228 (1), 19361279 (1)
colon cancer	63.4	31	12387883 (3), 16077033 (3), 12776194 (3), 15051827 (2) (see all 15)
tumors	38.5	62	11103818 (4), 16357510 (2), 19503094 (2), 19717984 (2) (see all 33)
carcinoma squamous cell	36.4	11	12439907 (2), 9553140 (1), 16357510 (1), 11103818 (1) (see all 8)
familial adenomatous polyposis	36	4	11521200 (2), 10913614 (1), 12776194 (1)
colorectal cancer	35.7	21	18264726 (5), 16814484 (5), 20385121 (2), 14724568 (2) (see all 6)
gastric cancer	33.3	18	15805274 (5), 17614846 (3), 15825076 (2), 17085651 (2) (see all 6)
cancer	32.8	37	12518228 (3), 12439907 (2), 18264726 (2), 16357509 (2) (see all 16)
pancreatic cancer	26.3	6	18559508 (4), 10859317 (1), 19823826 (1)
adenoma	24.1	15	18264726 (5), 10913614 (4), 17614846 (2), 17908689 (1) (see all 5)

Genetic Association Database (GAD): KLF4
Human Genome Epidemiology (HuGE) Navigator: KLF4 (3 documents)

Export disorders for KLF4 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for KLF4 gene, integrated from 9 sources (see all 257):
(articles sorted by number of sources associating them with KLF4)

Utopia: connect your pdf to the dynamic
world of online information

Human EZF, a Kruppel-like zinc finger protein, is expressed in vascular endothelial cells and contains transcriptional activation and repression domains. (PubMed id 9422764)^{1, 2, 3, 9} Yet S.-F....Lee M.-E. (1998)
KLF4, p21 and context-dependent opposing forces in cancer. (PubMed id 16372018)^{1, 3, 9} Rowland B.D. and Peeper D.S. (2006)
Cooperative transcriptional activation by Klf4, Meis2 , and Pbx1. (PubMed id 21746878)^{1, 2} Bjerke G.A....Wotton D. (2011)
Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. (PubMed id 20071344)^{1, 2} Zhang P....Lu W. (2010)
Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells. (PubMed id 18655026)^{1, 2} Tan F....Peng X. (2008)
Single nucleotide polymorphisms in the gene encoding Kruppel-like factor 7 are associated with type 2 diabetes. (PubMed id 15937668)^{1, 4} Kanazawa A....Maeda S. (2005)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Oncogene expression cloning by retroviral transduction of adenovirus E1A-immortalized rat kidney RK3E cells: transformation of a host with epithelial features by c-MYC and the zinc finger protein GKLF. (PubMed id 10392904)^{1, 2} Foster K.W.... Ruppert J.M. (1999)
Putative tumor-suppressive function of kruppel-like f actor 4 in primary lung carcinoma. (PubMed id 19737957)^{1, 9} Hu W....Fang B. (2009)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 9314	HGNC: 6348	AceView: KLF4	Ensembl:ENSG00000136826	euGenes: HUgn9314
ECgene: KLF4	H-InvDB: KLF4

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for KLF4	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for KLF4	Genetics and Cytogenetics in Oncology and Haematology
SeattleSNPs	http://pga.gs.washington.edu/data/klf4/

Intellectual Property for KLF4 gene
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Patent Information for KLF4 gene:
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GeneCards and IP:
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