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KIAA1303 Gene

protein-coding   GIFtS: 54

GC17P076134
raptor
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
Raptor 3
raptor 2
Descriptions
P150 target of rapamycin (TOR)-scaffold protein 3
p150 target of rapamycin (TOR)-scaffold protein containing
WD-repeats 2
regulatory associated protein of mTOR 2
External Ids
Entrez Gene: 575212
UniProtKB: Q8N1223
Ensembl: ENSG000001415647
Search outside databases for aliases for KIAA1303 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122
Function: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with
FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in
the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of
EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1.
Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity.
Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the
rate of cell growth and maintenance of cell size with different environmental conditions

Gene Wiki entry for KIAA1303

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KIAA1303 gene  

Entrez Gene cytogenetic band: 17q25.3   Ensembl cytogenetic band:  17q25.3   

KIAA1303 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P076134:     (about GC identifiers)

Start:
76,133,220 bp from pter
End:
76,554,768 bp from pter
Size:
421,549 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_024871.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122 (See protein sequence)
Recommended Name: Regulatory-associated protein of mTOR  
Size: 1335 amino acids; 149038 Da
Subunit: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds
preferentially to poorly or non-phosphorylated form of EIF4EBP1, and this binding is critical to
the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under
both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states
with different stability
Miscellaneous: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient
availability, and its potency for dissociation is increased under nutrient-rich conditions. This
action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its
intrinsic catalytic activity
Secondary accessions: Q8N4V9 Q8TB32 Q9P2P3
Alternative splicing: 2 isoforms:  Q8N122-1   Q8N122-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_065812.1  

    ENSEMBL proteins: 
    ENSP00000307272 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich CompoZr ZFN for KIAA1303  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Raptor)
    Human Recombinant Proteins from Abnova (raptor)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829 cytosol EXP15755954 15809305
    About this table

    Antibodies for KIAA1303: 
    Invitrogen Antibodies for KIAA1303
    Millipore Mono- and Polyclonal Antibodies for the study of KIAA1303
    Sigma-Aldrich Antibodies for KIAA1303
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for KIAA1303  (Raptor)
    Antibodies from Abcam (Raptor), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (raptor)
    Novus Biologicals Antibodies for RAPTOR

    Assays for KIAA1303: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR000357 HEAT
     IPR011989 ARM-like
     IPR019775 WD40_repeat_CS
     IPR017986 WD40_repeat_region
     IPR004083 Raptor


       GeneDecks  KIAA1303 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8N122

    ProtoNet protein and cluster: Q8N122

    2 Blocks protein families:
    IPB000357 HEAT repeat
    IPB004083 Saccharomyces cerevisiae 175.8kDa hypothetical protein signature


    UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122
    Similarity: Belongs to the WD repeat RAPTOR family
    Similarity: Contains 7 WD repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KIAA1303)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020761

                  Applied Biosystems Silencer® siRNAs for KIAA1303

                  Sigma-Aldrich siRNA for KIAA1303  
                         Sigma-Aldrich shRNA for KIAA1303  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for KIAA1303
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020761
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020761
                                     untagged cDNA clone in CMV expression vector: NM_020761 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_020761

    UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122
    Function: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with
    FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in
    the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of
    EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1.
    Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity.
    Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the
    rate of cell growth and maintenance of cell size with different environmental conditions

    13 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Rptor):

    adipose tissuebehavior/neurologicalcellulardigestive/alimentaryembryogenesis
    growth/sizehomeostasis/metabolismlethality-postnatallethality-prenatal/perinatallife span-post-weaning/aging
    musclereproductive systemskeleton

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005488 binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  KIAA1303  (Your Favorite Gene powered by Ingenuity) 
     Insulin Receptor Signaling

       GeneDecks  KIAA1303 for the pathways selected above  
    About GeneDecksing

    2 Cell Signaling Technology (CST) Pathways for KIAA1303: 
     Translational Control: Regulation of eIF2
     Insulin Receptor Signaling

       GeneDecks  KIAA1303 for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for KIAA1303):
     hsa04150 mTOR signaling pathway
     hsa04910 Insulin signaling pathway

       GeneDecks  KIAA1303 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  KIAA1303 


    5/28 Interacting proteins for KIAA1303 (ENSP000003072723 Q8N1222) via UniProtKB, MINT, and/or STRING (see all 28 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EIF4EBP1Q135412STRING (score=.999) MINT-6549354
    RPS6KB1ENSP000002255773STRING (score=.999)
    FKBP1AENSP000003711383STRING (score=.998)
    AKT1S1ENSP000003416983STRING (score=.996)
    GBLENSP000003017243STRING (score=.996)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for KIAA1303

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KIAA1303)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020761

                  Sigma-Aldrich siRNA for KIAA1303  
                         Sigma-Aldrich shRNA for KIAA1303  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_020761  

    REFSEQ mRNAs for KIAA1303 gene: 

    NM_020761.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_020761  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020761
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020761
                                     untagged cDNA clone in CMV expression vector: NM_020761 

    Additional cDNA sequence: 

    AB037724.1 AB082951.1 AK055912.1 AK295964.1 AK296321.1 AY090663.1 BC014502.1 BC025180.1 
    BC033258.1 BC033699.1 BC041079.1 BC064515.1 BC073898.1 BC136652.1 BC136654.1 CR608664.1 

    9 DOTS entries:

    DT.413296  DT.100749451  DT.100836007  DT.100758660  DT.65285420  DT.100001522  DT.95274839  DT.97772244 
    DT.95274834 

    24/165 AceView cDNA sequences (see all 165 ):

    BC064515 BM725017 AI028378 CR608664 BU153684 BM474228 BU187764 CF272460 
    CA426914 BM981742 BM680224 BM809091 BM718442 BM455531 BC073898 BM556894 
    CA310481 BM854064 BM914430 AA629126 AB082951 BV202131 AA470470 BM069709 

    highest scoring ESTs for KIAA1303:

    H06513 AA349796 AA353858 AA400088 AA470470 AA603730 AA629126 AA745735 AA783040 AB037724 

    Unigene Cluster for KIAA1303:

    Raptor
    Hs.133044  [show with all ESTs]
    Unigene Representative Sequence: NM_020761


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000306801  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    KIAA1303 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for KIAA1303

    1 / 2 / 3

    3 probe-sets matching KIAA1303 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    56274_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 H06513 1.00 0.90 0.95 1

    225715_at2, 3 U133-B 1 1.00 1.00 -- -- AB037724 0.80 0.88 0.84 1

    225715_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  KIAA1303 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.133044

    UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122
    Tissue specificity: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung,
    small intestine, kidney and placenta

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for KIAA1303 gene from 5/18 species (see all 18 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC6083781   -- similar to raptor 88.86(n)
    95.96(a)
    608378  XM_845394.1  XP_850487.1 
    chimpanzee
    (Pan troglodytes)
    LOC4549491   -- raptor 99.3(n)
    99.55(a)
    454949  XM_001161716.1  XP_001161716.1 
    cow
    (Bos taurus)
    LOC5070561   -- similar to raptor 89.36(n)
    96.23(a)
    507056  XM_583606.3  XP_583606.3 
    rat
    (Rattus norvegicus)
    RGD13117841   -- similar to p150 target of rapamycin (TOR)-scaffold more 87.64(n)
    96.78(a)
    287871  XM_213539.4  XP_213539.3 
    mouse
    (Mus musculus)
    Rptor5
    4932417H02Rik1
    115
    regulatory associated protein of MTOR, complex 15
    RIKEN cDNA 4932417H02 gene1
    87.42(n)1
    96.55(a)1
    743701  NM_028898.11  NP_083174.11 
     AB0829525  AK0165305  (see all 22)
    About this table        Species with no ortholog for KIAA1303

    ENSEMBL Gene Tree for KIAA1303
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/3423 NCBI SNPs in KIAA1303 are shown (see all 3423 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 984)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10629351,2
    A,C,F,H,O76554452(+) GTTTCT/CGTTTG 1 -- ut31 ese36Minor allele frequency- C:0.48EA MN EU WA 2054
    rs48900401,2
    A,C,F,H76133003(+) ATAATG/AACAAT 1 -- ng517Minor allele frequency- A:0.35NA EU EA WA 550
    rs37519321,2
    A,C,F,H76554009(-) TGGTCA/GTGGGC 1 -- ut3113Minor allele frequency- G:0.12EA EU WA NA 2532
    rs10456261,2
    A,C,F,H76554559(+) GGCACC/TGCGTG 1 -- ut315Minor allele frequency- T:0.48EA EU WA 1876
    rs37519341,2
    A,C,F,H76553093(-) CTGTCG/TGGGGG 1 -- ut315Minor allele frequency- T:0.44EA EU WA 1914
    rs80757101,2
    C,F,H76133717(+) CCCCTC/TGCAGC 1 -- ut51 ese34Minor allele frequency- T:0.10EU EA WA 416
    rs126028851,2
    C,F,H76133764(+) GCTTGG/AGACCT 1 -- ut518Minor allele frequency- A:0.20EU EA WA 826
    rs72249411,2
    C,F,H76131503(+) CTGGTG/AACTGT 1 -- ng518Minor allele frequency- A:0.02EU EA WA 838
    rs116586981,2
    C,F,H76132922(+) TGAAGC/TTTTTG 1 -- ng518Minor allele frequency- T:0.28EU EA WA NA 770
    rs48900391,2
    C,F,H76132478(+) CTCTTC/TTTCTG 1 -- ng51 trp37Minor allele frequency- T:0.25EU EA WA NA 558
    About this table

    HapMap Linkage Disequilibrium images for KIAA1303 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607130

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/62 PubMed articles for KIAA1303 gene (see all 62 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 57521 AceView: raptor Ensembl:ENSG00000141564 euGenes: HUgn57521
    (According to HUGE)
    About This Section

    HUGE: KIAA1303
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for KIAA1303:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Predesigned and custom siRNAs for KIAA1303 Antibodies for KIAA1303
     Explore super-pooled esiRNAs Proteins for KIAA1303
     Lentivirus-delivered shRNAs for KIAA1303 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Antibodies & Assays for KIAA1303  (Raptor)

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    (Raptor)
     Antibodies (Raptor)
     Search OriGene for KIAA1303
     Search Tocris compounds for KIAA1303




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for RAPTOR

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