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Aliases & Descriptions for KIAA1303
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| | | Descriptions |
|---|
| P150 target of rapamycin (TOR)-scaffold protein 3 | p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats 2 | | regulatory associated protein of mTOR 2 |
|
| | Search outside databases for aliases for KIAA1303 gene |
Summaries for KIAA1303(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122Function: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditionsGene Wiki entry for KIAA1303 |
Genomic Location for KIAA1303
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the KIAA1303 gene 
Entrez Gene cytogenetic band: 17q25.3 Ensembl cytogenetic band: 17q25.3 KIAA1303 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 17 GeneLoc Exon Structure GeneLoc location for GC17P076134:
(about GC identifiers)
Start:
|
76,133,220 bp from pter |
End:
|
76,554,768 bp from pter |
Size:
|
421,549 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000017.9 NT_024871.11
| Proteins for KIAA1303
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122 (See
protein sequence)Recommended Name: Regulatory-associated protein of mTOR Size: 1335 amino acids; 149038 Da
Subunit: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non-phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability
Miscellaneous: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity
Secondary accessions: Q8N4V9 Q8TB32 Q9P2P3Alternative splicing: 2 isoforms: Q8N122-1 Q8N122-2 Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_065812.1
ENSEMBL proteins: ENSP00000307272
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
1 Gene Ontology (GO) cellular component term (links to tree view): About this table
Antibodies for KIAA1303: Assays for KIAA1303: | Protein
Domains/ Families for KIAA1303(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q8N122
ProtoNet protein and cluster: Q8N122 2 Blocks protein families: IPB000357 HEAT repeat IPB004083 Saccharomyces cerevisiae 175.8kDa hypothetical protein signature
UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122Similarity: Belongs to the WD repeat RAPTOR familySimilarity: Contains 7 WD repeats | Gene Function for KIAA1303
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020761
Applied Biosystems Silencer® siRNAs for KIAA1303
Sigma-Aldrich siRNA for KIAA1303  Sigma-Aldrich shRNA for KIAA1303  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020761                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_020761                                  untagged cDNA clone in CMV expression vector: NM_020761 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_020761
UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122Function: Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions13 MGI mutant phenotypes (inferred from 3 alleles ) (MGI details for Rptor):
1 Gene Ontology (GO) molecular function term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005488 | binding |
IEA | -- | About this table | Pathways & Interactions for KIAA1303
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| 1 Sigma-Aldrich "Your Favorite Gene" Pathway for KIAA1303 (Your Favorite Gene powered by Ingenuity) 
Gene Network CentralTM Interacting Genes and Proteins Network for KIAA1303 
5/28 Interacting proteins for KIAA1303 (ENSP000003072723 Q8N1222) via UniProtKB, MINT, and/or STRING (see all 28
)About this table
|
Drugs & Compounds for KIAA1303(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for KIAA1303
|
Transcripts for KIAA1303(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020761
Sigma-Aldrich siRNA for KIAA1303  Sigma-Aldrich shRNA for KIAA1303  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_020761 REFSEQ mRNAs for KIAA1303 gene: NM_020761.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_020761               OriGene GFP tagged cDNA clone in CMV expression vector: NM_020761                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_020761                                  untagged cDNA clone in CMV expression vector: NM_020761  Additional cDNA sequence: AB037724.1 AB082951.1 AK055912.1 AK295964.1 AK296321.1 AY090663.1 BC014502.1 BC025180.1 BC033258.1 BC033699.1 BC041079.1 BC064515.1 BC073898.1 BC136652.1 BC136654.1 CR608664.1 9 DOTS entries: DT.413296 DT.100749451 DT.100836007 DT.100758660 DT.65285420 DT.100001522 DT.95274839 DT.97772244 DT.95274834 24/165 AceView cDNA sequences (see all 165
):BC064515 BM725017 AI028378 CR608664 BU153684 BM474228 BU187764 CF272460 CA426914 BM981742 BM680224 BM809091 BM718442 BM455531 BC073898 BM556894 CA310481 BM854064 BM914430 AA629126 AB082951 BV202131 AA470470 BM069709
highest scoring ESTs for KIAA1303:H06513 AA349796 AA353858 AA400088 AA470470 AA603730 AA629126 AA745735 AA783040 AB037724 Unigene Cluster for KIAA1303: Raptor Hs.133044 [show with all ESTs]Unigene Representative Sequence: NM_020761
GeneLoc Exon Structure
1 Ensembl transcript including schematic representation: ENST00000306801
|
Expression for KIAA1303
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| KIAA1303 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for KIAA1303
1 / 2 / 3 3 probe-sets matching KIAA1303 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene cluster: Hs.133044 UniProtKB/Swiss-Prot: RPTOR_HUMAN, Q8N122Tissue specificity: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta |
Orthologs for KIAA1303
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for KIAA1303 gene from 5/18 species (see all 18
)
About this table Species with no ortholog for KIAA1303
ENSEMBL Gene Tree for KIAA1303 | Paralogs for KIAA1303(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| -- |
SNPs/Variants for KIAA1303(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for KIAA1303 (up to first 250kb)
|
Disorders & Mutations for KIAA1303
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 607130
|
Medical News for KIAA1303(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for KIAA1303 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/62 PubMed articles for KIAA1303 gene (see all 62
):- Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function. (PubMed id 15066126)1, 3, 4 Oshiro N.... Yonezawa K. (2004)
- Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action. (PubMed id 12150926)1, 3, 4 Hara K.... Yonezawa K. (2002)
- mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the growth machinery. (PubMed id 12150925)1, 3, 4 Kim D.-H.... Sabatini D.M. (2002)
- A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)3, 4 Beausoleil S.A.... Gygi S.P. (2006)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)3, 4 Olsen J.V....Mann M. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Large-scale characterization of HeLa cell nuclear phosphoproteins. (PubMed id 15302935)3, 4 Beausoleil S.A....Gygi S.P. (2004)
- Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10718198)3, 4 Nagase T....Ohara O. (2000)
- Oncogenic MAPK signaling stimulates mTORC1 activity by promoting RSK-mediated raptor phosphorylation. (PubMed id 18722121)1, 3 Carriere A....Roux P.P. (2008)
- GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. (PubMed id 12718876)1, 3 Kim D.H....Sabatini D.M. (2003)
|
Search for KIAA1303
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing KIAA1303
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing KIAA1303
(According to HUGE)
About This Section
| |
Specialized Databases showing KIAA1303(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for KIAA1303(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for KIAA1303:

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| Antibodies & Assays for KIAA1303  (Raptor) |
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GeneCards Homepage - Last full update: 1 Jul 2009
Incremental update: 13 Oct 2009
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