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KCNF1 Gene

protein-coding   GIFtS: 56

GC02P011002
potassium voltage-gated channel, subfamily F, member 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: KCNF)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
IK8 1, 2
KCNF 2
KH1 5
KV5.1 2
Kv5.1 1
MGC33316 2
kH1 1, 2, 3
Descriptions
Voltage-gated potassium channel subunit Kv5.1 3
potassium channel KH1 2
potassium channel Kv5.1 2
potassium voltage-gated channel, subfamily F, member 1 2
External Ids
HGNC: 62461
Entrez Gene: 37542
UniProtKB: Q9H3M03
Ensembl: ENSG000001629757
Search outside databases for aliases for KCNF1 gene

Previous GC identifers: GC02P010837 GC02P011103 GC02P011073

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for KCNF1:
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion
channels from both functional and structural standpoints. Their diverse functions include
regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability,
epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a
member of the potassium channel, voltage-gated, subfamily F. This gene is intronless and expressed
in all tissues tested, including the heart, skeletal muscle, brain, kidney, and pancreas.
[provided by RefSeq]

UniProtKB/Swiss-Prot: KCNF1_HUMAN, Q9H3M0
Function: Putative voltage-gated potassium channel

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KCNF1 gene  

Entrez Gene cytogenetic band: 2p25   Ensembl cytogenetic band:  2p25.1   HGNC cytogenetic band: 2p25

KCNF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P011002:     (about GC identifiers)

Start:
10,969,514 bp from pter
End:
10,971,802 bp from pter
Size:
2,289 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005334.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: KCNF1_HUMAN, Q9H3M0 (See protein sequence)
Recommended Name: Potassium voltage-gated channel subfamily F member 1  
Size: 494 amino acids; 55584 Da
Subunit: Heteromultimer with KCNG3, KCNG4 and KCNV2. Interacts with DLG1
Subcellular location: Membrane; Multi-pass membrane protein
Secondary accessions: O43527

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_002227.2  

    ENSEMBL proteins: 
    ENSP00000295082 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (KCNF1)
    Human Recombinant Proteins from Abnova (KCNF1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008076 voltage-gated potassium channel complex IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for KCNF1: 
    Browse Antibodies Central at Invitrogen
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    Sigma-Aldrich Antibodies for KCNF1
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    Antibodies from Abcam (KCNF1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (KCNF1)
    Novus Biologicals Antibodies for KCNF1

    Assays for KCNF1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/7 InterPro domains/families (see all 7 ):
     IPR005821 Ion_trans
     IPR000210 BTB/POZ-like
     IPR011333 BTB/POZ_fold
     IPR003971 K_chnl_volt-dep_Kv9
     IPR003968 K_chnl_volt-dep_Kv


       GeneDecks  KCNF1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H3M0

    ProtoNet protein and cluster: Q9H3M0

    3 Blocks protein families:
    IPB000210 BTB/POZ domain
    IPB003091 Potassium channel signature
    IPB003971 Kv9 potassium channel alpha subunit signature


    UniProtKB/Swiss-Prot: KCNF1_HUMAN, Q9H3M0
    Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of
    positively charged amino acids at every third position
    Similarity: Belongs to the potassium channel family. F subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCNF1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCNF1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002236

                  Applied Biosystems Silencer® siRNAs for KCNF1

                  Sigma-Aldrich siRNA and siRNA Panels for KCNF1  
                         Sigma-Aldrich shRNA Panels and shRNA for KCNF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002236
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002236
                                     untagged cDNA clone in CMV expression vector: NM_002236 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_002236

    UniProtKB/Swiss-Prot: KCNF1_HUMAN, Q9H3M0
    Function: Putative voltage-gated potassium channel

    Genatlas biochemistry entry for KCNF1:
    potassium voltage-gated channel,subfamily G,member 2,rat K13 homolog,expressed in
    brain,placenta,skeletal muscle with an alternatively spliced isoform,also expressed in skeletal
    muscle
    1 MGI phenotypic allele for Kcnf1 (no phenotypes)

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005244 voltage-gated ion channel activity IEA--
    GO:0005249 voltage-gated potassium channel activity IEA--
    GO:0005515 protein binding IEA--
    GO:0030955 potassium ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  KCNF1 

      --

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006811 ion transport IEA--
    GO:0006813 potassium ion transport TAS9434767
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Compounds for KCNF1 available from Tocris Bioscience
    CompoundAction CAS number
    AM 92016 hydrochlorideKV channel blocker[178894-81-0]
    KN-93CaM kinase II inhibitor. Also K+ channel blocker (KV)[139298-40-1]
    SG 209K+ channel opener[83440-03-3]
    4-AminopyridineK+ channel blocker[504-24-5]
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCNF1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCNF1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002236

                  Sigma-Aldrich siRNA and siRNA Panels for KCNF1  
                         Sigma-Aldrich shRNA Panels and shRNA for KCNF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_002236  

    REFSEQ mRNAs for KCNF1 gene: 

    NM_002236.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_002236  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002236
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002236
                                     untagged cDNA clone in CMV expression vector: NM_002236 

    Additional cDNA sequence: 

    AF029780.1 AF033382.1 BC026110.2 

    3 DOTS entries:

    DT.110435  DT.100745018  DT.92416967 

    24/25 AceView cDNA sequences (see all 25 ):

    AI274992 NM_002236 AF029780 BF516107 Z38223 AI942483 AI361583 CB054271 
    AF033382 BG708997 CB054272 BC026110 F04496 BM714346 AA316752 BV183403 
    BI757855 BQ613980 AA984726 BQ549847 R21482 R45529 F05186 CB155571 

    highest scoring ESTs for KCNF1:

    AF033382 AA316752 AA984726 AF029780 AI274992 AI361583 BC026110 BF516107 BG708997 BI757855 

    Unigene Cluster for KCNF1:

    Potassium voltage-gated channel, subfamily F, member 1
    Hs.23735  [show with all ESTs]
    Unigene Representative Sequence: BC026110


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000295082  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    KCNF1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for KCNF1

    1 / 2 / 3

    3 probe-sets matching KCNF1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    39268_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF033382 1.00 1.00 1.00 1

    210263_at2, 3 U133-A 1 1.00 1.00 -- -- AF029780 0.80 1.00 0.91 1

    210263_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  KCNF1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TCATCGACAG

    SOURCE GeneReport for Unigene cluster: Hs.23735

    Expression variation in blood from EXPOLDB for KCNF1

    UniProtKB/Swiss-Prot: KCNF1_HUMAN, Q9H3M0
    Tissue specificity: Detected in heart, brain, liver, skeletal muscle, kidney and pancreas

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for KCNF1 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    KCNF11   -- potassium voltage-gated channel, subfamily F, member more 93.93(n)
    96.15(a)
    482968  XM_540081.1  XP_540081.1 
    cow
    (Bos taurus)
    KCNF11   -- potassium voltage-gated channel, subfamily F, member more 92.38(n)
    96.56(a)
    505879  XM_582243.3  XP_582243.2 
    rat
    (Rattus norvegicus)
    Kcnf11   -- potassium voltage-gated channel, subfamily F, member more 88.91(n)
    96.55(a)
    298908  XM_216678.4  XP_216678.4 
    mouse
    (Mus musculus)
    Kcnf11, 5 125
    potassium voltage-gated channel, subfamily F, member more1, 5 89.45(n)1
    96.55(a)1
    3825711  NM_201531.21  NP_963289.11 
     AI6423215  AK1373675  (see all 9)
    chicken
    (Gallus gallus)
    KCNF11   -- potassium voltage-gated channel, subfamily F, member more 75.86(n)
    85.8(a)
    428652  XM_426210.2  XP_426210.2 
    About this table        Species with no ortholog for KCNF1

    ENSEMBL Gene Tree for KCNF1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for KCNF1 gene
    KCNV22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/22 NCBI SNPs in KCNF1 are shown (see all 22 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 29)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs67172261,2
    F,H10968135(+) tattcT/Caatat 1 -- ng514Minor allele frequency- C:0.02EU EA WA 418
    --
    rs126171631,2
    A,C10969672(+) GACCCC/TGCTCC 1 -- ut51 ese30--------
    rs37321051,2
    A,C,F,H10971191(+) GTCATC/TGCCAT 1 I/I syn112Minor allele frequency- T:0.47EA EU WA NA 2360
    rs37321041,2
    A,C,F10970471(+) GACGCG/AGCCGA 1 A/A syn11Minor allele frequency- A:0.16NA 70
    --
    rs596206741,2
    --10971763(+) CGCCCA/GCTTCT 1 -- ut310--------
    rs10460041,2
    --10971070(+) TAAGAA/GCATCC 1 S/N mis1 ese30--------
    --
    rs341060121,2
    --10967587(+) ACCTA-/AGGGTG 1 -- ng510--------
    rs10460051,2
    --10971150(+) CGCTGG/AGCAAG 1 S/G mis11Minor allele frequency- A:0.00MN 184
    --
    rs351990851,2
    --10971500(+) GGCCC-/CTCAGG 1 -- ut310--------
    --
    rs18826511,2
    --10967622(-) TGTGTC/GGAAAT 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for KCNF1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603787

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for KCNF1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 3754 HGNC: 6246 AceView: KCNF1 Ensembl:ENSG00000162975 euGenes: HUgn3754
    ECgene: KCNF1 H-InvDB: KCNF1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for KCNF1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for KCNF1 Antibodies for KCNF1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for KCNF1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for KCNF1

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