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KCNE4 Gene

protein-coding   GIFtS: 57

GC02P223625
potassium voltage-gated channel, Isk-related family, member 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
MGC20353 2
MIRP3 2, 5
MiRP3 1
Descriptions
MinK-related peptide 3 2, 3
Minimum potassium ion channel-related peptide 3 2, 3
Potassium channel subunit beta MiRP3 3
cardiac voltage-gated potassium channel accessory subunit 4 2
potassium voltage-gated channel subfamily E member 4 2
potassium voltage-gated channel, Isk-related family, member
4 2
External Ids
HGNC: 62441
Entrez Gene: 237042
UniProtKB: Q8WWG93
Ensembl: ENSG000001520497
Search outside databases for aliases for KCNE4 gene

Previous GC identifers: GC02P221939 GC02P222637 GC02P223880 GC02P224119 GC02P223742

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for KCNE4:
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion
channels from both functional and structural standpoints. Their diverse functions include
regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability,
epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a
member of the potassium channel, voltage-gated, isk-related subfamily. This member is a type I
membrane protein, and a beta subunit that assembles with a potassium channel alpha-subunit to
modulate the gating kinetics and enhance stability of the multimeric complex. This gene is
prominently expressed in the embryo and in adult uterus. [provided by RefSeq]

UniProtKB/Swiss-Prot: KCNE4_HUMAN, Q8WWG9
Function: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel
complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of
the channel complex. May associate with KCNQ1/KVLTQ1 and inhibit potassium current

Gene Wiki entry for KCNE4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KCNE4 gene  

Entrez Gene cytogenetic band: 2q36.3   Ensembl cytogenetic band:  2q36.1   HGNC cytogenetic band: 2q36.1

KCNE4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P223625:     (about GC identifiers)

Start:
223,625,106 bp from pter
End:
223,628,599 bp from pter
Size:
3,494 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: KCNE4_HUMAN, Q8WWG9 (See protein sequence)
Recommended Name: Potassium voltage-gated channel subfamily E member 4  
Size: 170 amino acids; 18439 Da
Subunit: Associates with KCNQ1/KVLQT1 (Probable)
Subcellular location: Membrane; Single-pass type I membrane protein
Secondary accessions: Q53SM4 Q96CC4

REFSEQ proteins: NP_542402.2  

ENSEMBL proteins: 
ENSP00000281830 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (KCNE4)
Human Recombinant Proteins from Abnova (KCNE4)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005856 cytoskeleton IDA18029348
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
GO:0043231 intracellular membrane-bounded organelle IDA18029348
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Antibodies for KCNE4: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for KCNE4
Browse R&D Systems for Antibodies
Antibodies from Abcam (KCNE4), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (KCNE4)
Novus Biologicals Antibody for KCNE4

Assays for KCNE4: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR000369 K_chnl_volt-dep_bsu_KCNE


   GeneDecks  KCNE4 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q8WWG9

ProtoNet protein and cluster: Q8WWG9

1 Blocks protein family: IPB000369 KCNE voltage-gated K+ channel beta subunit family signature

UniProtKB/Swiss-Prot: KCNE4_HUMAN, Q8WWG9
Similarity: Belongs to the potassium channel KCNE family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (KCNE4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (KCNE4)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_080671

              Applied Biosystems Silencer® siRNAs for KCNE4

              Sigma-Aldrich siRNA for KCNE4  
                     Sigma-Aldrich shRNA for KCNE4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for KCNE4
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_080671
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_080671
                                 untagged cDNA clone in CMV expression vector: NM_080671 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_080671

UniProtKB/Swiss-Prot: KCNE4_HUMAN, Q8WWG9
Function: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel
complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of
the channel complex. May associate with KCNQ1/KVLTQ1 and inhibit potassium current

1 MGI phenotypic allele for Kcne4 (no phenotypes)


3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005244 voltage-gated ion channel activity IEA--
GO:0005249 voltage-gated potassium channel activity IEA--
GO:0030955 potassium ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  KCNE4 


5 Interacting proteins for KCNE4 (ENSP000002818303) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
KCNQ1ENSP000003701533STRING (score=.992)
KCNE3ENSP000003105573STRING (score=.88)
KCNA1ENSP000002288583STRING (score=.841)
KCNE2ENSP000002903103STRING (score=.768)
KCNE1LENSP000003611733STRING (score=.761)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006811 ion transport IEA--
GO:0006813 potassium ion transport IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Compounds for KCNE4 available from Tocris Bioscience
CompoundAction CAS number
AM 92016 hydrochlorideKV channel blocker[178894-81-0]
KN-93CaM kinase II inhibitor. Also K+ channel blocker (KV)[139298-40-1]
SG 209K+ channel opener[83440-03-3]
4-AminopyridineK+ channel blocker[504-24-5]
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1 PharmGKB drug compound relationship for KCNE4 gene
Drug compound PharmGKB Relations PubMed IDs for articles supporting these relationships
antiarrhythmicsFA  12096056
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (KCNE4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (KCNE4)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_080671

              Sigma-Aldrich siRNA for KCNE4  
                     Sigma-Aldrich shRNA for KCNE4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_080671  

REFSEQ mRNAs for KCNE4 gene: 

NM_080671.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_080671  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_080671
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_080671
                                 untagged cDNA clone in CMV expression vector: NM_080671 

Additional cDNA sequence: 

AK294423.1 AK313203.1 AY065987.1 BC014429.1 DQ192293.1 

3 DOTS entries:

DT.97840581  DT.100834171  DT.97806316 

24/50 AceView cDNA sequences (see all 50 ):

AI287239 BM820427 AA730156 AA058640 CK818864 BX324837 BX381340 BQ216886 
BP348485 BX327955 BM126272 BC014429 AA479033 CA390179 BQ421396 BQ718246 
BF793600 AY065987 BX499846 AI375138 CN481584 BI756417 BX327956 AI002715 

highest scoring ESTs for KCNE4:

AA730156 AI375138 AW748651 AY065987 BC014429 BF793600 BG330322 BQ215885 BQ216886 BQ420572 

Unigene Cluster for KCNE4:

Potassium voltage-gated channel, Isk-related family, member 4
Hs.348522  [show with all ESTs]
Unigene Representative Sequence: NM_080671


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000281830  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

KCNE4 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for KCNE4

1 / 2 / 3

5 probe-sets matching KCNE4 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
74565_at2, 3 U95-E 1 1.00 1.00 1.00 1.00 AA479033 0.20 1.00 0.72 1

222379_at2, 3 U133-A 1 1.00 1.00 -- -- AI002715 0.20 1.00 0.72 1

222379_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1552508_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1552507_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  KCNE4 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGCCTTGTCA

SOURCE GeneReport for Unigene cluster: Hs.348522

UniProtKB/Swiss-Prot: KCNE4_HUMAN, Q8WWG9
Tissue specificity: Predominantly expressed in embryo and adult uterus. Low expression found in
kidney, small intestine, lung and heart

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for KCNE4 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
KCNE41   -- potassium voltage-gated channel, Isk-related family, more 91.76(n)
91.76(a)
488549  XM_545668.2  XP_545668.1 
chimpanzee
(Pan troglodytes)
LOC7444781   -- hypothetical protein LOC744478 99.22(n)
99.41(a)
744478  XM_001166586.1  XP_001166586.1 
cow
(Bos taurus)
KCNE41   -- potassium voltage-gated channel, Isk-related family, more 88.1(n)
88.69(a)
615761  XM_879929.2  XP_885022.1 
rat
(Rattus norvegicus)
Kcne41   -- potassium voltage-gated channel, Isk-related subfamily, more 87.25(n)
90.59(a)
367302  NM_212526.1  NP_997691.1 
mouse
(Mus musculus)
Kcne41, 5 15
potassium voltage-gated channel, Isk-related subfamily, more1, 5 86.67(n)1
90(a)1
578141  NM_021342.11  NP_067317.11 
 AF0765335  AK1334695  (see all 10)
About this table        Species with no ortholog for KCNE4

ENSEMBL Gene Tree for KCNE4
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/86 NCBI SNPs in KCNE4 are shown (see all 86 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 35)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs126216431,2
A,C,F,H223626227(+) GACGAG/TGAGCT 1 D/E mis113Minor allele frequency- T:0.30EU EA WA NS 1924
--
rs76024951,2
C,F,H223624324(+) GCCTCC/G/TGCATG 1 -- ng518EU EA WA NS 794
rs101897621,2
A,C,F223626324(+) CCTGCG/CGGTGG 1 -- ut31 ese34Minor allele frequency- C:0.04NS 374
rs37958851,2
C,F,H223626577(-) CAGAAC/TCCCAA 1 -- ut31 ese36Minor allele frequency- T:0.04EA EU WA NS 2014
rs37958841,2
C,F,H223626607(-) GGCAAG/ACATAT 1 -- ut31 ese38Minor allele frequency- A:0.04EU EA WA NS 794
--
rs102084291,2
A,C223627918(+) GGGTTC/TCAGCG 1 -- ut310--------
--
rs101723801,2
A,C223627981(+) CGCCCA/GGCTAA 1 -- ut310--------
--
rs101833671,2
C,F223624532(+) ACCAGC/GCAGCC 1 -- ng514Minor allele frequency- G:0.09NS 374
--
rs130308681,2
C,F223628685(+) TCCTCT/CCCCAA 1 -- ng313Minor allele frequency- C:0.02NS 278
rs130318251,2
C,H223624926(+) TCATCG/ATCACC 1 -- ng515Minor allele frequency- A:0.01EU EA WA NA 504
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HapMap Linkage Disequilibrium images for KCNE4 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607775

Human Gene Mutation Database: KCNE4
Human Genome Epidemiology Navigator: KCNE4 (2 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/20 PubMed articles for KCNE4 gene (see all 20 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 23704 HGNC: 6244 AceView: KCNE4 Ensembl:ENSG00000152049 euGenes: HUgn23704
ECgene: KCNE4 H-InvDB: KCNE4
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for KCNE4:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for KCNE4-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for KCNE4 Antibodies for KCNE4
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for KCNE4 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins
(KCNE4)
 Antibodies (KCNE4)
 Search OriGene for KCNE4
 Tocris compounds for KCNE4




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for KCNE4

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