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KCNB2 Gene

protein-coding   GIFtS: 62

GC08P073612
potassium voltage-gated channel, Shab-related subfamily, member 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
KV2.2 2, 5
Kv2.2 1
Descriptions
Voltage-gated potassium channel subunit Kv2.2 3
delayed rectifier potassium channel protein 2
potassium channel Kv2.2 2
potassium voltage-gated channel subfamily B member 2 2
potassium voltage-gated channel, Shab-related subfamily,
member 2 2
External Ids
HGNC: 62321
Entrez Gene: 93122
UniProtKB: Q929533
Ensembl: ENSG000001826747
Search outside databases for aliases for KCNB2 gene

Previous GC identifers: GC08P072532 GC08P073420 GC08P073172 GC08P073499

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for KCNB2:
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion
channels from both functional and structural standpoints. Their diverse functions include
regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability,
epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four
sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in
Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the
potassium channel, voltage-gated, shab-related subfamily. This member is a delayed rectifier
potassium channel. The gene is expressed in gastrointestinal smooth muscle cells. [provided by
RefSeq]

UniProtKB/Swiss-Prot: KCNB2_HUMAN, Q92953
Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes.
Channels open or close in response to the voltage difference across the membrane, letting
potassium ions pass in accordance with their electrochemical gradient

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KCNB2 gene  

Entrez Gene cytogenetic band: 8q13.2   Ensembl cytogenetic band:  8q13.3   HGNC cytogenetic band: 8q13.2

KCNB2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P073612:     (about GC identifiers)

Start:
73,612,180 bp from pter
End:
74,013,138 bp from pter
Size:
400,959 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000008.9  NT_008183.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: KCNB2_HUMAN, Q92953 (See protein sequence)
Recommended Name: Potassium voltage-gated channel subfamily B member 2  
Size: 911 amino acids; 102563 Da
Subunit: Heteromultimer with KCNS1, KCNS2 and KCNS3 (By similarity)
Subcellular location: Membrane; Multi-pass membrane protein
Sequence caution: Sequence=AAB08433.1; Type=Frameshift; Positions=768;
Secondary accessions: Q7Z7D0 Q9BXD3

Post-translational modifications:

  • Phosphorylated (Potential)1


  • REFSEQ proteins: NP_004761.2  

    ENSEMBL proteins: 
    ENSP00000329426 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Kv2.2)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008076 voltage-gated potassium channel complex TAS9612272
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for KCNB2: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of KCNB2
    Browse Antibodies at Sigma-Aldrich
    R&D Systems Antibodies for KCNB2 (Potassium Channel Kv2.2)
    Antibodies from Abcam (Kv2.2), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for KCNB2

    Assays for KCNB2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR005821 Ion_trans
     IPR000210 BTB/POZ-like
     IPR011333 BTB/POZ_fold
     IPR003973 K_chnl_volt-dep_Kv2
     IPR005826 K_chnl_volt-dep_Kv2.2


       GeneDecks  KCNB2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q92953

    ProtoNet protein and cluster: Q92953

    3 Blocks protein families:
    IPB000210 BTB/POZ domain
    IPB003091 Potassium channel signature
    IPB005826 Kv2.2 voltage-gated K+ channel signature


    UniProtKB/Swiss-Prot: KCNB2_HUMAN, Q92953
    Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of
    positively charged amino acids at every third position
    Domain: The tail may be important in modulation of channel activity and/or targeting of the channel
    to specific subcellular compartments
    Similarity: Belongs to the potassium channel family. B (Shab) subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCNB2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCNB2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004770

                  Applied Biosystems Silencer® siRNAs for KCNB2

                  Sigma-Aldrich siRNA and siRNA Panels for KCNB2  
                         Sigma-Aldrich shRNA Panels and shRNA for KCNB2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004770
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004770
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_004770 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004770

    UniProtKB/Swiss-Prot: KCNB2_HUMAN, Q92953
    Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes.
    Channels open or close in response to the voltage difference across the membrane, letting
    potassium ions pass in accordance with their electrochemical gradient

    Genatlas biochemistry entry for KCNB2:
    potassium voltage-gated channel,Drosophila Shab-related subfamily,member 2,expressed in (canine)
    gastrointestinal smooth muscles

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005244 voltage-gated ion channel activity IEA--
    GO:0005251 delayed rectifier potassium channel activity TAS9612272
    GO:0005515 protein binding IPI17474147
    GO:0030955 potassium ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for KCNB2
      outward current

       GeneDecks  KCNB2 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  KCNB2 


    5/45 Interacting proteins for KCNB2 (ENSP000003294263 Q929531) via UniProtKB, MINT, and/or STRING (see all 45 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    GRB2P629931EBI-1760439, EBI-401755
    KCNJ1ENSP000003161363STRING (score=.968)
    KCNJ4ENSP000003064973STRING (score=.959)
    KCNIP2ENSP000003478773STRING (score=.952)
    KCNE2ENSP000002903103STRING (score=.95)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006811 ion transport IEA--
    GO:0006813 potassium ion transport TAS9612272
    GO:0006940 regulation of smooth muscle contraction TAS9612272
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Compounds for KCNB2 available from Tocris Bioscience
    CompoundAction CAS number
    AM 92016 hydrochlorideKV channel blocker[178894-81-0]
    KN-93CaM kinase II inhibitor. Also K+ channel blocker (KV)[139298-40-1]
    SG 209K+ channel opener[83440-03-3]
    4-AminopyridineK+ channel blocker[504-24-5]
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCNB2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCNB2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004770

                  Sigma-Aldrich siRNA and siRNA Panels for KCNB2  
                         Sigma-Aldrich shRNA Panels and shRNA for KCNB2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004770  

    REFSEQ mRNAs for KCNB2 gene: 

    NM_004770.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004770  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004770
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004770
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_004770 

    Additional cDNA sequence: 

    AF338730.1 AF450111.1 AK311263.1 U69962.1 

    1 DOTS entry:

    DT.92033542 

    5 AceView cDNA sequences:

    NM_004770 AF338730 AF450111 U69962 CK004440 

    highest scoring ESTs for KCNB2:

    U69962 AF338730 AF450111 CK004440 NM_004770 DA618105 AA017044 AA017045 AA332929 AA416661 

    Unigene Cluster for KCNB2:

    Potassium voltage-gated channel, Shab-related subfamily, member 2
    Hs.661102  [show with all ESTs]
    Unigene Representative Sequence: AF338730


    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for KCNB2

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5
    SP1:                              
    SP2:        -     -     -         

    About this scheme

    ECgene alternative splicing isoforms for KCNB2

    1 Ensembl transcript including schematic representation:
    ENST00000329185  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    KCNB2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for KCNB2

    1 / 2 / 3

    5 probe-sets matching KCNB2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    31312_at2, 3 U95-A 2 1.00 0.97 1.00 1.00 U69962 1.00 1.00 1.00 1

    208172_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004770 0.60 1.00 0.82 1

    208123_at2, 3 U133-A 1 1.00 1.00 -- -- AF338730 0.80 1.00 0.91 1

    208172_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    208123_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AACAGTTTAG

    SOURCE GeneReport for Unigene cluster: Hs.661102

    Expression variation in blood from EXPOLDB for KCNB2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for KCNB2 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    KCNB21   -- potassium voltage-gated channel, Shab-related subfamily, more 83.58(n)
    90.62(a)
    403925  NM_001003247.1  NP_001003247.1 
    chimpanzee
    (Pan troglodytes)
    KCNB21   -- potassium voltage-gated channel, Shab-related subfamily, more 99.63(n)
    99.89(a)
    472792  XM_528164.2  XP_528164.2 
    cow
    (Bos taurus)
    KCNB21   -- potassium voltage-gated channel, Shab-related subfamily, more 86.03(n)
    92.52(a)
    535990  NM_001024563.1  NP_001019734.1 
    rat
    (Rattus norvegicus)
    Kcnb21   -- potassium voltage gated channel, Shab-related subfamily, more 86.5(n)
    92.78(a)
    117105  NM_054000.1  NP_446452.1 
    mouse
    (Mus musculus)
    Kcnb21, 5 1 (1.00 cM)5
    potassium voltage gated channel, Shab-related subfamily, more1, 5 87.06(n)1
    93.05(a)1
    987411  XM_001473804.11  XP_001473854.11 
     AK0362395  AK0532775  (see all 8)
    About this table        Species with no ortholog for KCNB2

    ENSEMBL Gene Tree for KCNB2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for KCNB2 gene
    KCNB12  KCNV12  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/2077 NCBI SNPs in KCNB2 are shown (see all 2077 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 815)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 8 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs169385081,2
    C,F,H74013366(+) AACAAA/GTTTCT 1 -- ng317Minor allele frequency- G:0.40NA EU EA WA 556
    rs169385071,2
    C,F,H74012114(+) CAGAGA/GGAAAG 1 E/G mis1 ese3 trp37Minor allele frequency- G:0.01NA EU EA WA 546
    --
    rs3493361,2
    A73610607(-) gtgatA/Gttccc 1 -- ng510--------
    --
    rs78450901,2
    C73612494(+) ACGCCA/GGGAGC 1 -- ut510--------
    rs117821181,2
    C,F,H74012757(+) GCTGTG/AAGTGA 1 V/V syn18Minor allele frequency- A:0.21NA EU EA WA 606
    rs342591571,2
    F73642679(+) AGAACG/TCTGGA 1 T/T syn11Minor allele frequency- T:0.04NA 72
    rs21281561,2
    A,C,F,H,O73992537(+) TTTTGC/TGGTAT 1 -- int17Minor allele frequency- T:0.44NA EU EA WA 554
    rs22452611,2
    A,C,F,H,O73840916(+) AGGGAC/TACCTT 1 -- int112Minor allele frequency- N:0.00EU EA WA NA 1054
    rs4067981,2
    A,C,F,H,O73627458(-) CTCATG/CAAAGT 1 -- int17Minor allele frequency- C:0.47NA EU EA WA 560
    rs14403381,2
    A,C,F,H,O73889668(-) TCTCTA/GTAATT 1 -- int111Minor allele frequency- G:0.30NA EU EA WA 980
    About this table

    HapMap Linkage Disequilibrium images for KCNB2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607738

    Human Genome Epidemiology Navigator: KCNB2 (2 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/13 PubMed articles for KCNB2 gene (see all 13 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9312 HGNC: 6232 AceView: KCNB2 Ensembl:ENSG00000182674 euGenes: HUgn9312
    ECgene: KCNB2 H-InvDB: KCNB2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for KCNB2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for KCNB2 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for KCNB2 Browse small molecules at Sigma-Aldrich
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     Search Novus for antibodies for KCNB2

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