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KCNA6 Gene

protein-coding   GIFtS: 57

GC12P004788
potassium voltage-gated channel, shaker-related subfamily, member 6
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLJ25134 2
HBK2 1, 2, 3
KV1.6 2
Kv1.6 1
Descriptions
Voltage-gated potassium channel subunit Kv1.6 3
human brain potassium channel-2 2
potassium voltage-gated channel, shaker-related subfamily,
member 6 2
voltage-gated potassium channel protein Kv1.6 2
External Ids
HGNC: 62251
Entrez Gene: 37422
UniProtKB: P176583
Ensembl: ENSG000001510797
Search outside databases for aliases for KCNA6 gene

Previous GC identifers: GC12P004655 GC12P004810

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for KCNA6:
Potassium channels represent the most complex class of voltage-gated ion channels from both
functional and structural standpoints. Their diverse functions include regulating neurotransmitter
release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport,
smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes -
shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have
human homolog(s). This gene encodes a member of the potassium channel, voltage-gated,
shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type
repeat in the fourth segment. It belongs to the delayed rectifier class. The coding region of this
gene is intronless, and the gene is clustered with genes KCNA1 and KCNA5 on chromosome 12.
[provided by RefSeq]

UniProtKB/Swiss-Prot: KCNA6_HUMAN, P17658
Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes.
Assuming opened or closed conformations in response to the voltage difference across the membrane,
the protein forms a potassium-selective channel through which potassium ions may pass in
accordance with their electrochemical gradient

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KCNA6 gene  

Entrez Gene cytogenetic band: 12p13   Ensembl cytogenetic band:  12p13.32   HGNC cytogenetic band: 12p13

KCNA6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P004788:     (about GC identifiers)

Start:
4,788,603 bp from pter
End:
4,792,839 bp from pter
Size:
4,237 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_009759.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: KCNA6_HUMAN, P17658 (See protein sequence)
Recommended Name: Potassium voltage-gated channel subfamily A member 6  
Size: 529 amino acids; 58729 Da
Subunit: Heterotetramer of potassium channel proteins (By similarity)
Subcellular location: Membrane; Multi-pass membrane protein

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_002226.1  

    ENSEMBL proteins: 
    ENSP00000280684 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Millipore Purified and/or Recombinant KCNA6 Protein
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Kv1.6)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008076 voltage-gated potassium channel complex TAS2347305
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for KCNA6: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of KCNA6
    Sigma-Aldrich Antibodies for KCNA6
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Kv1.6), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for KCNA6

    Assays for KCNA6: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR005821 Ion_trans
     IPR000210 BTB/POZ-like
     IPR004053 K_chnl_volt-dep_Kv1.6
     IPR011333 BTB/POZ_fold
     IPR003972 K_chnl_volt-dep_Kv1


       GeneDecks  KCNA6 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P17658

    ProtoNet protein and cluster: P17658

    4 Blocks protein families:
    IPB000210 BTB/POZ domain
    IPB003091 Potassium channel signature
    IPB003972 Skaker voltage-gated K+ channel family signature
    IPB004053 Kv1.6 voltage-gated K+ channel signature


    UniProtKB/Swiss-Prot: KCNA6_HUMAN, P17658
    Domain: The N-terminus may be important in determining the rate of inactivation of the channel
    while the tail may play a role in modulation of channel activity and/or targeting of the channel
    to specific subcellular compartments
    Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of
    positively charged amino acids at every third position
    Similarity: Belongs to the potassium channel family. A (Shaker) subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCNA6)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCNA6)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002235

                  Applied Biosystems Silencer® siRNAs for KCNA6

                  Sigma-Aldrich siRNA and siRNA Panels for KCNA6  
                         Sigma-Aldrich shRNA Panels and shRNA for KCNA6  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for KCNA6
    Millipore Clones for the Expression of KCNA6
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002235
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002235
                                     untagged cDNA clone in CMV expression vector: NM_002235 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_002235

    UniProtKB/Swiss-Prot: KCNA6_HUMAN, P17658
    Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes.
    Assuming opened or closed conformations in response to the voltage difference across the membrane,
    the protein forms a potassium-selective channel through which potassium ions may pass in
    accordance with their electrochemical gradient

    Genatlas biochemistry entry for KCNA6:
    potassium voltage-gated channel,Drosophila shaker-related subfamily,member 6 (mouse Kv1.6 homolog)

    3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Kcna6):

    behavior/neurologicalhomeostasis/metabolismtouch/vibrissae

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005244 voltage-gated ion channel activity IEA--
    GO:0005249 voltage-gated potassium channel activity TAS2347305
    GO:0005515 protein binding IEA--
    GO:0030955 potassium ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for KCNA6
      outward current

       GeneDecks  KCNA6 for the pathways selected above  
    About GeneDecksing


    5/44 Interacting proteins for KCNA6 (ENSP000002806843) via UniProtKB, MINT, and/or STRING (see all 44 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    KCNH2ENSP000002621863STRING (score=.981)
    KCNJ1ENSP000003161363STRING (score=.954)
    KCNJ3ENSP000002951013STRING (score=.953)
    KCNE1ENSP000003372553STRING (score=.943)
    KCNJ5ENSP000003399603STRING (score=.942)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006811 ion transport IEA--
    GO:0006813 potassium ion transport TAS2347305
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for KCNA6
    Browse Small Molecules at Sigma-Aldrich

    Compounds for KCNA6 available from Tocris Bioscience
    CompoundAction CAS number
    Chromanol 293BIKs blocker. Also blocks ICFTR[163163-23-3]
    AM 92016 hydrochlorideKV channel blocker[178894-81-0]
    KN-93CaM kinase II inhibitor. Also K+ channel blocker (KV)[139298-40-1]
    (-)-[3R,4S]-Chromanol 293BIKs blocker. Enantiomer of (Cat. No. 1412)[163163-24-4]
    4-AminopyridineK+ channel blocker[504-24-5]
    About this table


    1 Novoseek chemical compound relationship for KCNA6 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    potassium 47.41 2 8719753 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCNA6)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCNA6)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002235

                  Sigma-Aldrich siRNA and siRNA Panels for KCNA6  
                         Sigma-Aldrich shRNA Panels and shRNA for KCNA6  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_002235  

    REFSEQ mRNAs for KCNA6 gene: 

    NM_002235.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_002235  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_002235
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_002235
                                     untagged cDNA clone in CMV expression vector: NM_002235 

    Additional cDNA sequence: AK057863.1 

    6 DOTS entries:

    DT.100017225  DT.442806  DT.121205686  DT.121205638  DT.121205631  DT.91694726 

    24/60 AceView cDNA sequences (see all 60 ):

    AJ505959 BM719843 BC069355 AJ271385 BM852999 AA609028 BQ774134 CD724101 
    AA242896 CA442989 NM_017417 BX090709 AA812749 AK057863 AA082821 AA082828 
    CB153065 AI655634 BM984255 AA083815 BM971346 BQ128051 AW005440 BF057823 

    highest scoring ESTs for KCNA6:

    X17622 BC069355 BE700671 BF115418 BF930901 BX501195 CB153065 R15156 T28887 BI039546 

    Unigene Clusters for KCNA6:

    Potassium voltage-gated channel, shaker-related subfamily, member 6
    Hs.306190  [show with all ESTs], Hs.426162  [show with all ESTs]
    Unigene Representative Sequences: NM_002235, AK057863


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000280684  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    KCNA6 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for KCNA6

    1 / 2 / 3

    4 probe-sets matching KCNA6 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    32473_at2, 3 U95-A 3 1.00 0.94 1.00 1.00 X17622 1.00 0.90 0.95 1

    205616_at2, 3 U133-A 1 0.82 1.00 -- -- AW134812 0.40 0.75 0.60 1

    1553347_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    205616_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene clusters: Hs.306190 Hs.426162

    Expression variation in blood from EXPOLDB for KCNA6

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for KCNA6 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    KCNA61   -- potassium voltage-gated channel, shaker-related subfamily, more 99.05(n)
    99.24(a)
    747272  XM_001159166.1  XP_001159166.1 
    cow
    (Bos taurus)
    KCNA61   -- potassium voltage-gated channel, shaker-related subfamily, more 91.33(n)
    96.2(a)
    539714  XM_588936.3  XP_588936.2 
    rat
    (Rattus norvegicus)
    Kcna61   -- potassium voltage gated channel, shaker related subfamily, more 86.45(n)
    92.82(a)
    64358  XM_575671.2  XP_575671.1 
    mouse
    (Mus musculus)
    Kcna61, 5 6 (61.00 cM)5
    potassium voltage-gated channel, shaker-related, subfamily, more1, 5 86.3(n)1
    93.18(a)1
    164941  NM_013568.51  NP_038596.11 
     AK0318905  AK1344775  (see all 9)
    zebrafish
    (Danio rerio)
    LOC7954901   -- similar to shaker-related potassium channel Tsha2 66.33(n)
    74.56(a)
    795490  XM_001335643.2  XP_001335679.2 
    About this table        Species with no ortholog for KCNA6

    ENSEMBL Gene Tree for KCNA6
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for KCNA6 gene
    KCNA12  KCNA22  KCNA32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/47 NCBI SNPs in KCNA6 are shown (see all 47 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 58)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs22395031,2
    A,C,F,H,O4791489(+) CAGGCT/CACTTG 1 -- ut319Minor allele frequency- C:0.45EA EU WA 2330
    --
    rs7004801,2
    A,C,F,H,O4830350(+) ACCCGT/CTGTCC 1 -- ut318Minor allele frequency- C:0.49MN NA EU EA WA 744
    rs7585331,2
    A,C,F,H4789171(+) AGCCAG/ATTTCA 1 -- ut519Minor allele frequency- A:0.44NA EA MN EU WA 1074
    --
    rs123361,2
    A,C,F,H4830378(+) AATGTT/CGGGGT 1 -- ut319Minor allele frequency- C:0.48MN NA EU EA WA 932
    rs33191,2
    A,C,F,H4792291(+) TGGAGC/GTGCAT 1 -- ut316Minor allele frequency- G:0.44EA MN EU WA 1510
    rs22395041,2
    A,C,F,H4791562(+) AACAAT/CCACTG 1 -- ut314Minor allele frequency- C:0.44EU EA WA 412
    --
    rs140541,2
    A,C,F,H4830284(+) TCTGGA/CCTGAC 1 -- ut315Minor allele frequency- C:0.49MN EU EA WA 590
    rs22395051,2
    C,F,H4791950(+) AGGTGC/TCACTT 1 -- ut3115Minor allele frequency- T:0.27EA NA EU WA 2924
    rs20582051,2
    A,C,F4793958(+) ATTTTT/GTCAAG 1 -- ut311Minor allele frequency- G:0.47EA 1104
    --
    rs31683771,2
    A,C,F4830254(+) GAGAAA/CCCTGC 1 -- ut311Minor allele frequency- C:0.29MN 186
    About this table

    HapMap Linkage Disequilibrium images for KCNA6 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 176257

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/13 PubMed articles for KCNA6 gene (see all 13 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 3742 HGNC: 6225 AceView: KCNA6andGALNT8 Ensembl:ENSG00000151079 euGenes: HUgn3742
    ECgene: KCNA6 H-InvDB: KCNA6
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for KCNA6:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for KCNA6-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for KCNA6 Antibodies for KCNA6
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for KCNA6 Browse small molecules at Sigma-Aldrich
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for KCNA6

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