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KCNA10 Gene

protein-coding   GIFtS: 51

GC01M110772
potassium voltage-gated channel, shaker-related subfamily, member 10
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
Kcn1 2
Kv1.8 1, 2
OTTHUMP00000024387 2
Descriptions
Voltage-gated potassium channel subunit Kv1.8 3
cyclic GMP gated potassium channel 2
potassium voltage-gated channel, shaker-related subfamily,
member 10 2
External Ids
HGNC: 62191
Entrez Gene: 37442
UniProtKB: Q163223
Ensembl: ENSG000001431057
Search outside databases for aliases for KCNA10 gene

Previous GC identifers: GC01M111476 GC01M109926 GC01M110161 GC01M110358

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for KCNA10:
Potassium channels represent the most complex class of voltage-gated ion channels from both
functional and structural standpoints. Their diverse functions include regulating neurotransmitter
release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport,
smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes -
shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have
human homolog(s). This gene encodes a member of the potassium channel, voltage-gated,
shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type
repeat in the fourth segment. It is specifically regulated by cGMP and postulated to mediate the
effects of substances that increase intracellular cGMP. This gene is intronless, and the gene is
clustered with genes KCNA2 and KCNA3 on chromosome 1. [provided by RefSeq]

UniProtKB/Swiss-Prot: KCA10_HUMAN, Q16322
Function: Mediates voltage-dependent potassium ion permeability of excitable membranes. Assuming
opened or closed conformations in response to the voltage difference across the membrane, the
protein forms a potassium-selective channel through which potassium ions may pass in accordance
with their electrochemical gradient. The channel activity is up-regulated by cAMP

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KCNA10 gene  

Entrez Gene cytogenetic band: 1p13.1   Ensembl cytogenetic band:  1p13.3   HGNC cytogenetic band: 1p13.1

KCNA10 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M110772:     (about GC identifiers)

Start:
110,861,362 bp from pter
End:
110,863,320 bp from pter
Size:
1,959 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_019273.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: KCA10_HUMAN, Q16322 (See protein sequence)
Recommended Name: Potassium voltage-gated channel subfamily A member 10  
Size: 511 amino acids; 57785 Da
Subunit: Homotetramer. Interacts with KCN4B/POMP. Interaction with KCN4B/POMP is necessary for the
modulation of channel activity by cAMP
Subcellular location: Membrane; Multi-pass membrane protein

REFSEQ proteins: NP_005540.1  

ENSEMBL proteins: 
ENSP00000358786 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Millipore Purified and/or Recombinant KCNA10 Protein
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (KCNA10)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008076 voltage-gated potassium channel complex IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for KCNA10: 
Browse Antibodies Central at Invitrogen
Millipore Mono- and Polyclonal Antibodies for the study of KCNA10
Sigma-Aldrich Antibodies for KCNA10
Browse R&D Systems for Antibodies
Antibodies from Abcam (KCNA10), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for KCNA10

Assays for KCNA10: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/7 InterPro domains/families (see all 7 ):
 IPR005821 Ion_trans
 IPR000210 BTB/POZ-like
 IPR011333 BTB/POZ_fold
 IPR003972 K_chnl_volt-dep_Kv1
 IPR003968 K_chnl_volt-dep_Kv


   GeneDecks  KCNA10 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q16322

ProtoNet protein and cluster: Q16322

3 Blocks protein families:
IPB000210 BTB/POZ domain
IPB003091 Potassium channel signature
IPB003972 Skaker voltage-gated K+ channel family signature


UniProtKB/Swiss-Prot: KCA10_HUMAN, Q16322
Domain: The N-terminus may be important in determining the rate of inactivation of the channel
while the tail may play a role in modulation of channel activity and/or targeting of the channel
to specific subcellular compartments
Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of
positively charged amino acids at every third position
Similarity: Belongs to the potassium channel family. A (Shaker) subfamily

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (KCNA10)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (KCNA10)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005549

              Applied Biosystems Silencer® siRNAs for KCNA10

              Sigma-Aldrich siRNA and siRNA Panels for KCNA10  
                     Sigma-Aldrich shRNA Panels and shRNA for KCNA10  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Millipore Clones for the Expression of KCNA10
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005549
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005549
                                 untagged cDNA clone in CMV expression vector: NM_005549 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_005549

UniProtKB/Swiss-Prot: KCA10_HUMAN, Q16322
Function: Mediates voltage-dependent potassium ion permeability of excitable membranes. Assuming
opened or closed conformations in response to the voltage difference across the membrane, the
protein forms a potassium-selective channel through which potassium ions may pass in accordance
with their electrochemical gradient. The channel activity is up-regulated by cAMP

Genatlas biochemistry entry for KCNA10:
potassium voltage-gated channel,cGMP activated,Drosophila shaker-related subfamily,member 10

5 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005221 intracellular cyclic nucleotide activated cation channel activity TAS9177773
GO:0005244 voltage-gated ion channel activity IEA--
GO:0005249 voltage-gated potassium channel activity IEA--
GO:0005515 protein binding IEA--
GO:0030955 potassium ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Millipore Pathway for KCNA10
  outward current

   GeneDecks  KCNA10 for the pathways selected above  
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3 Interacting proteins for KCNA10 (ENSP000003587863) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
KCNH2ENSP000002621863STRING (score=.819)
KCNJ10ENSP000003570683STRING (score=.721)
KCNJ12ENSP000003281503STRING (score=.707)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006811 ion transport IEA--
GO:0006813 potassium ion transport TAS9177773
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for KCNA10

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (KCNA10)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (KCNA10)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005549

              Sigma-Aldrich siRNA and siRNA Panels for KCNA10  
                     Sigma-Aldrich shRNA Panels and shRNA for KCNA10  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_005549  

REFSEQ mRNAs for KCNA10 gene: 

NM_005549.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_005549  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005549
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005549
                                 untagged cDNA clone in CMV expression vector: NM_005549 

Additional cDNA sequence: 

BC069165.1 BC074990.2 

1 DOTS entry:

DT.91678539 

2 AceView cDNA sequences:

BC069165 CA309199 

highest scoring ESTs for KCNA10:

CA309199 BC069165 BC074990 NM_005549 

Unigene Cluster for KCNA10:

Potassium voltage-gated channel, shaker-related subfamily, member 10
Hs.248140
Unigene Representative Sequence: NM_005549


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000369771  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

KCNA10 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for KCNA10

1 / 2 / 3

2 probe-sets matching KCNA10 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

208560_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005549 0.60 1.00 0.82 1

208560_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AGACGGCCTT

SOURCE GeneReport for Unigene cluster: Hs.248140

UniProtKB/Swiss-Prot: KCA10_HUMAN, Q16322
Tissue specificity: Detected in kidney, in proximal tubules, glomerular endothelium, in vascular
endothelium and in smooth muscle cells

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for KCNA10 gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
KCNA101   -- potassium voltage-gated channel, shaker-related subfamily, more 90.31(n)
92.34(a)
490111  XM_547232.2  XP_547232.2 
chimpanzee
(Pan troglodytes)
KCNA101   -- potassium voltage-gated channel, shaker-related subfamily, more 99.22(n)
99.61(a)
469414  XM_524799.2  XP_524799.2 
cow
(Bos taurus)
KCNA101   -- potassium voltage-gated channel, shaker-related subfamily, more 91.52(n)
94.13(a)
538721  XM_582224.3  XP_582224.2 
rat
(Rattus norvegicus)
Kcna101   -- potassium voltage-gated channel, shaker-related subfamily, more 88.32(n)
92.37(a)
295360  XM_227577.4  XP_227577.2 
mouse
(Mus musculus)
Kcna101, 5 3 (53.00 cM)5
potassium voltage-gated channel, shaker-related subfamily, more1, 5 88.78(n)1
93.74(a)1
2421511  NM_001081140.11  NP_001074609.11 
 AK1579655  BC1378285  (see all 8)
About this table        Species with no ortholog for KCNA10

ENSEMBL Gene Tree for KCNA10
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for KCNA10 gene
KCNA52  KCNA42  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/38 NCBI SNPs in KCNA10 are shown (see all 38 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs19364861,2
A,C,F,H,O110863977(-) TAGAAT/AGAACC 1 -- ng318Minor allele frequency- A:0.20EU EA WA NA 632
rs22510031,2
A,C,F,H110864629(+) CTGCTG/CTGCAA 1 -- ng318Minor allele frequency- C:0.40EU EA WA 792
rs37684561,2
C,F,H110863176(-) AGTTAT/CTAGTT 1 -- ut51 tfbs38Minor allele frequency- C:0.28EA NA EU WA 2012
rs170259571,2
C,F,H110863572(+) CCAATC/TGACAA 1 -- ng31 tfbs37Minor allele frequency- T:0.16NA EU EA WA 558
rs349708571,2
C,F110862275(+) GACCAC/TGGCCA 1 V/M mis15Minor allele frequency- T:0.07NA EU EA WA 484
--
rs22629291,2
C110863888(+) gtgtgC/TgCACG 1 -- ng310--------
rs37487291,2
H110862160(+) TCTTGC/TTCATG 1 S/N mis15Minor allele frequency- T:0.01EA EU WA 1144
--
rs37684571,2
C110861348(-) TCTCTC/TTCTCT 1 -- ng510--------
--
rs38418461,2
C110861335(-) CTCTC-/TCGCTTT 1 -- ng510--------
rs118068121,2
C110862172(+) TGAGAC/TTGGGG 1 N/S mis1 ese30--------
About this table

HapMap Linkage Disequilibrium images for KCNA10 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 602420

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/11 PubMed articles for KCNA10 gene (see all 11 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 3744 HGNC: 6219 AceView: KCNA10.1 Ensembl:ENSG00000143105 euGenes: HUgn3744
ECgene: KCNA10 H-InvDB: KCNA10
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for KCNA10:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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 Predesigned and custom siRNAs for KCNA10 Antibodies for KCNA10
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 Search Novus for antibodies for KCNA10

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