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KCMF1 Gene

protein-coding   GIFtS: 50

GC02P085109
potassium channel modulatory factor 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DEBT91 1, 2
DKFZP434L1021 1
DKFZp434L1021 2
EC 6.3.2.- 3
FIGC 2, 3
PCMF 1, 2, 3
ZZZ1 1, 2, 3
Descriptions
FGF-induced in gastric cancer 3
FGF-induced ubiquitin-protein ligase in gastric cancers 2
Potassium channel modulatory factor 3
ZZ-type zinc finger-containing protein 1 3
differentially expressed in branching tubulogenesis 91 2
potassium channel modulatory factor 1 2
zinc finger, ZZ domain containing 1 2
External Ids
HGNC: 205891
Entrez Gene: 568882
UniProtKB: Q9P0J73
Ensembl: ENSG000001764077
Search outside databases for aliases for KCMF1 gene

Previous GC identifers: GC02P085155 GC02P085173

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: KCMF1_HUMAN, Q9P0J7
Function: Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the KCMF1 gene  

Entrez Gene cytogenetic band: 2p11.2   Ensembl cytogenetic band:  2p11.2   HGNC cytogenetic band: 2p11.2

KCMF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P085109:     (about GC identifiers)

Start:
85,051,727 bp from pter
End:
85,140,106 bp from pter
Size:
88,380 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: KCMF1_HUMAN, Q9P0J7 (See protein sequence)
Recommended Name: E3 ubiquitin-protein ligase KCMF1  
Size: 381 amino acids; 41945 Da
Secondary accessions: Q4ZG04 Q53SC7 Q9BWK2 Q9H8P5 Q9UFE8

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_064507.3  

    ENSEMBL proteins: 
    ENSP00000319986 ENSP00000386738 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (PCMF)
    Human Recombinant Proteins from Abnova (KCMF1)
                  OriGene Purified Recombinant Human Protein: KCMF1 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IEA--
    About this table

    Antibodies for KCMF1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for KCMF1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (PCMF), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibody for KCMF1

    Assays for KCMF1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR015880 Znf_C2H2-like
     IPR007087 Znf_C2H2
     IPR000433 Znf_ZZ


       GeneDecks  KCMF1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9P0J7

    ProtoNet protein and cluster: Q9P0J7

    1 Blocks protein family: IPB000433 Zn-finger

    UniProtKB/Swiss-Prot: KCMF1_HUMAN, Q9P0J7
    Similarity: Belongs to the KCMF1 family
    Similarity: Contains 1 C2H2-type zinc finger
    Similarity: Contains 1 ZZ-type zinc finger

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCMF1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCMF1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020122

                  Applied Biosystems Silencer® siRNAs for KCMF1

                  Sigma-Aldrich siRNA for KCMF1  
                         Sigma-Aldrich shRNA for KCMF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for KCMF1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020122
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020122
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_020122 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_020122

    UniProtKB/Swiss-Prot: KCMF1_HUMAN, Q9P0J7
    Function: Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination
    Induction: Up-regulated by basic fibroblast growth factor (bFGF) in gastric cancer cells
    Enzyme Number (IUBMB): EC 6.3.2.- 

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008270 zinc ion binding IEA--
    GO:0016874 ligase activity IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0019941 modification-dependent protein catabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for KCMF1
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for KCMF1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (KCMF1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (KCMF1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_020122

                  Sigma-Aldrich siRNA for KCMF1  
                         Sigma-Aldrich shRNA for KCMF1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_020122  

    REFSEQ mRNAs for KCMF1 gene: 

    NM_020122.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_020122  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_020122
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_020122
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_020122 

    Additional cDNA sequence: 

    AB083199.1 AF131740.1 AF155652.1 AK023403.1 AK311099.1 AK314761.1 AL122115.1 BC000178.2 
    CR607426.1 CR617449.1 CR619076.1 

    10 DOTS entries:

    DT.85105570  DT.435421  DT.102824014  DT.65287093  DT.100792772  DT.95168284  DT.100792768  DT.100792769 
    DT.75146766  DT.95168286 

    24/229 AceView cDNA sequences (see all 229 ):

    AW043666 AA778328 CA489095 AA256696 BI868238 BM976032 BG109888 BQ774080 
    N90901 CB128521 BP381341 CA396812 CD677198 BQ024012 BG618288 AI341578 
    AA676549 AA825824 BU844982 BM981196 AA481311 BE904985 CB143942 CR607426 

    highest scoring ESTs for KCMF1:

    AA131134 AA181257 AA213566 AA243698 AA255803 AA256696 AA369285 AA376721 AA436378 AA481311 

    Unigene Cluster for KCMF1:

    Potassium channel modulatory factor 1
    Hs.654968  [show with all ESTs]
    Unigene Representative Sequence: NM_020122


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000319855  ENST00000409785  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    KCMF1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for KCMF1

    1 / 2 / 3

    12 probe-sets matching KCMF1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    79466_at2 U95-D 1 1.00 1.00 0.70 0.87 -- -- -- -- --
    91454_at2, 3 U95-D 1 1.00 1.00 0.65 0.82 AI221300 0.20 1.00 0.72 1

    65898_at2, 3 U95-C 1 1.00 1.00 0.88 1.12 AI743396 0.80 1.00 0.91 1

    45811_at2, 3 U95-B 1 1.00 1.00 0.22 1.20 AA131134 1.00 1.00 1.00 1

    217938_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_020122 0.60 1.00 0.82 1

    222471_s_at2, 3 U133-B 1 1.00 1.00 -- -- AI743396 0.80 1.00 0.91 1

    240430_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    242887_at2, 3 U133-B 1 0.91 1.00 -- -- AI221300 0.20 1.00 0.72 1

    217938_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222471_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    240430_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    242887_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
    GeneDecks  KCMF1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CAAAAGGCTC

    SOURCE GeneReport for Unigene cluster: Hs.654968

    UniProtKB/Swiss-Prot: KCMF1_HUMAN, Q9P0J7
    Tissue specificity: Spleen, small intestine, ovary, peripheral blood, lung, kidney and pancreas.
    Expressed at low levels in the thymus, prostate, testis, colon, heart, brain, placenta and liver

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for KCMF1 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    KCMF11   -- potassium channel modulatory factor 1 96.59(n)
    98.39(a)
    475773  XM_532983.2  XP_532983.2 
    chimpanzee
    (Pan troglodytes)
    KCMF11   -- potassium channel modulatory factor 1 99.91(n)
    100(a)
    459357  XM_515581.2  XP_515581.2 
    cow
    (Bos taurus)
    KCMF11   -- potassium channel modulatory factor 1 96.85(n)
    99.21(a)
    613522  NM_001076275.1  NP_001069743.1 
    mouse
    (Mus musculus)
    Kcmf11, 5 6 (30.50 cM)5
    potassium channel modulatory factor 11, 5 94.05(n)1
    97.38(a)1
    742871  NM_019715.21  NP_062689.21 
     AF1438595  AK0070705  (see all 16)
    chicken
    (Gallus gallus)
    KCMF11   -- potassium channel modulatory factor 1 89.63(n)
    93.88(a)
    770239  XM_001233571.1  XP_001233572.1 
    About this table        Species with no ortholog for KCMF1

    ENSEMBL Gene Tree for KCMF1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/247 NCBI SNPs in KCMF1 are shown (see all 247 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 59)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs67307971,2
    A,C,F,H85137261(+) GGCTGC/GAGAAA 1 -- ut317Minor allele frequency- G:0.03EU EA WA NA 562
    rs48321311,2
    A,C,F,H85137224(+) GTAGAG/AGGAGA 1 -- ut318Minor allele frequency- A:0.49EU EA WA 838
    rs22766081,2
    C,F,H85136557(-) ACTGTA/GGAACA 1 -- ut314Minor allele frequency- G:0.12EU EA WA 420
    rs177618571,2
    C,F,H85135364(+) AGGTGC/GGCACA 1 -- ut319Minor allele frequency- G:0.01NA EU EA WA 744
    rs22725141,2
    C,F,H85140030(+) TGATAT/GAGATT 1 -- ut3113Minor allele frequency- G:0.25EA NS NA EU WA 1956
    rs22725121,2
    C,F,H85139960(+) CTGACG/CATACA 1 -- ut3117Minor allele frequency- C:0.23EA NS NA EU WA 2564
    rs22725131,2
    C,F,H85139993(+) TTGTAT/ACAGGC 1 -- ut3113Minor allele frequency- A:0.27EA NS NA EU WA 2136
    rs170258371,2
    F,H85136390(+) GATGTA/GCTATT 1 -- ut317Minor allele frequency- G:0.19NA EU EA WA 562
    rs134115931,2
    H85139253(+) TTCCCA/CGTAGG 1 -- ut314Minor allele frequency- C:0.00EU EA WA 416
    rs115543361,2
    H85124253(+) GGATGA/CCTTTG 1 D/A mis1 ese34Minor allele frequency- C:0.00EU EA WA 420
    About this table

    HapMap Linkage Disequilibrium images for KCMF1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10 PubMed articles for KCMF1 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 56888 HGNC: 20589 AceView: KCMF1 Ensembl:ENSG00000176407 euGenes: HUgn56888
    ECgene: KCMF1 H-InvDB: KCMF1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for KCMF1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for KCMF1-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for KCMF1 Antibodies for KCMF1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for KCMF1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
    (PCMF)
     Antibodies (PCMF)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for KCMF1




     Drugs for KCMF1
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for KCMF1

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