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KANK2 Gene

protein-coding GIFtS: 47

GC19M011139

KN motif and ankyrin repeat domains 2
(Previous names: matrix-remodelling associated 3, ankyrin repeat domain 25 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: MXRA3, ANKRD25)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
ANKRD25 ^², ³
DKFZp434N161 ^²
FLJ20004 ^²
KIAA1518 ^¹, ², ³
MGC119707 ^²
MXRA3 ^², ³
SIP ^², ³

Descriptions
Ankyrin repeat domain-containing protein 25 ^³
KN motif and ankyrin repeat domains 2 ^²
Matrix-remodeling-associated protein 3 ^³
SRC-1 interacting protein ^²
SRC-1-interacting protein ^³
SRC1-interacting protein ^³
ankyrin repeat domain 25 ^¹, ²
kidney ankyrin repeat-containing protein 2 ^²
matrix-remodelling associated 3 ^¹, ²

External Ids
HGNC: 29300¹
Entrez Gene: 25959²
UniProtKB: Q63ZY3³
Ensembl: ENSG00000197256⁷

Search outside databases for aliases for KANK2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Gene Wiki entry for KANK2 (ANKRD25)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.2 Ensembl cytogenetic band: 19p13.2 HGNC cytogenetic band: 19p13.2

KANK2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure

GeneLoc location for GC19M011139: (about GC identifiers)

Start:	11,135,946 bp from pter
End:	11,167,496 bp from pter
Size:	31,551 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000019.8 NT_011295.10

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: KANK2_HUMAN, Q63ZY3 (See protein sequence)

Recommended Name: KN motif and ankyrin repeat domain-containing protein 2

Size: 851 amino acids; 91174 Da

Secondary accessions: B0I1P4 Q3KQZ3 Q6GUF5 Q9H8S4 Q9NUP0 Q9P210

Alternative splicing: 3 isoforms: Q63ZY3-1 Q63ZY3-2 Q63ZY3-3

Post-translational modifications:

Phosphorylated upon DNA damage, probably by ATM or ATR¹

View phosphorylation sites using PhosphoSite²

REFSEQ proteins (2 alternative transcripts):

NP_001129663.1 NP_056308.2

ENSEMBL proteins:

ENSP00000347276

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (ANKRD25)
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for KANK2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for KANK2
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (ANKRD25), each with their Abpromise^SM
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Novus Biologicals Antibodies for KANK2

Assays for KANK2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q63ZY3

ProtoNet protein and cluster: Q63ZY3

UniProtKB/Swiss-Prot: KANK2_HUMAN, Q63ZY3

Similarity: Contains 5 ANK repeats

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (KANK2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_015493

Applied Biosystems Silencer® siRNAs for KANK2

Sigma-Aldrich siRNA for KANK2

Sigma-Aldrich shRNA for KANK2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001136191
Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001136191
untagged cDNA clones in CMV expression vector (see all 3): NM_001136191

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_001136191

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

5/6 Interacting proteins for KANK2 (Q63ZY3² ENSP00000347276³) via UniProtKB, MINT, and/or STRING (see all 6 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
NCOA1	Q15788²	MINT-4958619 MINT-4958729 MINT-4958705 MINT-4958599
GRIP1	Q9Y3R0²	MINT-4958619 MINT-4958650
NCOA3	Q9Y6Q9²	MINT-4958619 MINT-4958650
CSNK2A1	P68400²	MINT-4961111
CSNK2A1P	P68400²	MINT-4961111

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for KANK2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (KANK2)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_015493

Sigma-Aldrich siRNA for KANK2

Sigma-Aldrich shRNA for KANK2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_001136191 NM_015493

REFSEQ mRNAs for KANK2 gene (2 alternative transcripts):

NM_001136191.1 NM_015493.5

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_001136191 NM_015493

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001136191
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001136191
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001136191

Additional cDNA sequence:

AB284125.1 AK000011.1 AK002094.1 AK225038.1 AL117489.1 AY639929.1 BC030030.2 BC032745.1

BC049201.1 BC082762.1 BC098105.1 BC098286.1 BC098312.1 BC105989.1 CR595669.1

16 DOTS entries:

DT.100796945 DT.418172 DT.95258828 DT.91737339 DT.121475625 DT.91746547 DT.121475644 DT.97842021

DT.95248411 DT.121475473 DT.121475528 DT.121475562 DT.91691142 DT.121475517 DT.121475551 DT.95116477

24/291 AceView cDNA sequences (see all 291 ):

BQ941266 BC082762 AK023332 AK002094 AU133959 AI571515 AI282589 AI753611

BQ212115 AW499863 BF063688 W84510 AA488007 H71470 AI628677 C01927

AA919076 AW069414 BX281094 CA390600 CF529080 AI492474 AA487895 W74274

Unigene Clusters for KANK2:

KN motif and ankyrin repeat domains 2

Hs.284208 [show with all ESTs], Hs.719195 [show with all ESTs]

Unigene Representative Sequences: BE896143, NM_015493

GeneLoc Exon Structure

5/10 Alternative Splicing Database (ASD) splice patterns (SP) for KANK2 (see all 10 )

ExUns:	1	^	2	^	3	^	4a	·	4b	·	4c	·	4d	^	5	^	6a	·	6b	^	7a	·	7b	·	7c	·	7d	·	7e	·	7f	^	8	^	9a	·	9b	·	9c	^	10	^	11	^	12a	·	12b	^	13	^	14	^
SP1:											-		-		-																				-																-
SP2:																																																			-
SP3:					-		-		-		-		-		-								-		-		-		-		-		-		-		-
SP4:
SP5:					-		-		-		-		-		-		-		-		-		-		-		-		-		-				-

ExUns:	15	^	16	^	17a	·	17b	·	17c
SP1:
SP2:
SP3:
SP4:
SP5:

About this scheme

ECgene alternative splicing isoforms for KANK2

1 Ensembl transcript including schematic representation:

ENST00000355150

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

KANK2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for KANK2

¹ / ²

5 probe-sets matching KANK2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
65720_at²	U95-C	1	1.00	1.00	1.00	1.00	--	--	--	--	--
221068_at²	U133-A	1	1.00	1.00	--	--	--	--	--	--	--
218418_s_at²	U133-A	1	1.00	1.00	--	--	--	--	--	--	--
221068_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
218418_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene clusters: Hs.284208 Hs.719195
UniProtKB/Swiss-Prot: KANK2_HUMAN, Q63ZY3
Tissue specificity: Strongly expressed in cervix, colon, heart, kidney and lung

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for KANK2 gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
cow (Bos taurus)	KANK2¹	--	KN motif and ankyrin repeat domains 2	86.88(n) 89.31(a)	767586 NM_001076531.1 NP_001069999.1
mouse (Mus musculus)	Kank2^{1, 5}	9⁵	KN motif and ankyrin repeat domains 2^{1, 5}	80.89(n)¹ 84.51(a)¹	235041¹ NM_145611.3¹ NP_663586.2¹ AI429529⁵ AI504612⁵ (see all 19)
zebrafish (Danio rerio)	BC056795.1²	--	--	77.51(n)	BC056795.1
African clawed frog (Xenopus laevis)	BC049177.1²	--	--	78.62(n)	BC049177.1

About this table Species with no ortholog for KANK2

ENSEMBL Gene Tree for KANK2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for KANK2 gene

KANK1² KANK4² KANK3²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/236 NCBI SNPs in KANK2 are shown (see all 236 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 66)

AB	SNP ID	Valid	Chr 19 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs7188^1,2	A,C,F,H,O	11136139(-)	`TACACT/GTCCTT`	2	--	ut3¹		9	MN NA EU EA WA	932
	rs7250778^1,2	A,C,F,H	11167265(+)	`TGCTGG/AGCATG`	1	--	ut5¹		4	EU EA WA	410
	rs7187^1,2	A,C,F,H	11136258(-)	`AAGAGT/CTCTGT`	2	--	ut3¹		5	MN EU EA	672
	rs4804572^1,2	A,C,F,H	11138074(+)	`GCCGTC/TGGGGC`	2	--	ut3¹ ese³		4	EU EA WA	412
	rs892112^1,2	A,C,F,H	11137744(-)	`ATCCCT/CCACAT`	2	--	ut3¹ ese³		13	EA MN EU WA NA	2444
	rs17616661^1,2	C,F,H	11164554(+)	`TCACCA/GTATGG`	2	M/T	mis¹		15	NA EU EA WA	1394
	rs755237^1,2	C,F,H	11165404(+)	`GCCACC/TGCGGG`	2	G/S	mis¹ ese³		8	NA EA MN EU WA	850
	rs3826812^1,2	C,F,H	11137621(+)	`TGAGTT/GTTCTG`	2	--	ut3¹		8	EA NA EU WA	2054
	rs892113^1,2	C,F,H	11136934(-)	`AACTTA/CAAGAG`	2	--	ut3¹		12	MN EU EA WA NA	1162
	rs754528^1,2	C,F,H	11166245(-)	`GCTGCA/GGGCAG`	2	--	ut5¹ ese³		8	NA EA MN EU WA	819

About this table

HapMap Linkage Disequilibrium images for KANK2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/16 PubMed articles for KANK2 gene (see all 16 ):

Kank proteins: a new family of ankyrin-repeat domain-containing proteins. (PubMed id 17996375)^{2, 3, 4} Zhu Y....Kiyama R. (2008)
Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10819331)^{2, 3, 4} Nagase T....Ohara O. (2000)
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{3, 4} Olsen J.V....Mann M. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)⁴ Dephoure N.... Gygi S.P. (2008)
SIP, a novel ankyrin repeat containing protein, sequesters steroid receptor coactivators in the cytoplasm. (PubMed id 17476305)³ Zhang Y....Shang Y. (2007)
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. (PubMed id 17525332)⁴ Matsuoka S.... Elledge S.J. (2007)
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)³ Kimura K.... Sugano S. (2006)
Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. (PubMed id 15146197)³ Brandenberger R.... Stanton L.W. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 25959	HGNC: 29300	AceView: ANKRD25	Ensembl:ENSG00000197256	euGenes: HUgn25959
ECgene: KANK2	H-InvDB: KANK2

(According to HUGE)
About This Section

HUGE: KIAA1518

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for KANK2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for KANK2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for KANK2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for KANK2		Antibodies for KANK2
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for KANK2		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Recombinant & Natural Proteins

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KANK2 Gene

protein-coding GIFtS: 47

Aliases &
Descriptions
for KANK2

Summaries
for KANK2

Genomic Location
for KANK2

Proteins
for KANK2

Protein Domains/
Families
for KANK2

Gene Function
for KANK2

Pathways & Interactions
for KANK2

Drugs & Compounds
for KANK2

Transcripts
for KANK2

Expression
for KANK2

Orthologs
for KANK2

Paralogs
for KANK2

SNPs/Variants
for KANK2

Disorders & Mutations
for KANK2

Medical News
for KANK2

Publications
for KANK2

Search for KANK2

Genome Databases showing KANK2

Other Databases showing KANK2

Specialized Databases showing KANK2

Licensable Technologies
for KANK2

Services
for KANK2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

KANK2 Gene

protein-coding GIFtS: 47

Aliases & Descriptionsfor KANK2

Summariesfor KANK2

Genomic Locationfor KANK2

Proteinsfor KANK2

Protein Domains/Familiesfor KANK2

Gene Functionfor KANK2

Pathways & Interactionsfor KANK2

Drugs & Compoundsfor KANK2

Transcriptsfor KANK2

Expression for KANK2

Orthologsfor KANK2

Paralogsfor KANK2

SNPs/Variantsfor KANK2

Disorders & Mutationsfor KANK2

Medical Newsfor KANK2

Publicationsfor KANK2

Search for KANK2

Genome Databases showing KANK2

Other Databases showing KANK2

Specialized Databases showing KANK2

Licensable Technologiesfor KANK2

Servicesfor KANK2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for KANK2

Summaries
for KANK2

Genomic Location
for KANK2

Proteins
for KANK2

Protein Domains/
Families
for KANK2

Gene Function
for KANK2

Pathways & Interactions
for KANK2

Drugs & Compounds
for KANK2

Transcripts
for KANK2

Expression
for KANK2

Orthologs
for KANK2

Paralogs
for KANK2

SNPs/Variants
for KANK2

Disorders & Mutations
for KANK2

Medical News
for KANK2

Publications
for KANK2

Licensable Technologies
for KANK2

Services
for KANK2