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JAM3 Gene

protein-coding   GIFtS: 60

GC11P133444
junctional adhesion molecule 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ14529 2
JAM-2 3
JAM-3 3
JAM-C 1, 2, 3
JAMC 1, 2
Descriptions
junctional adhesion molecule 3 2, 3
junctional adhesion molecule C 2
External Ids
HGNC: 155321
Entrez Gene: 837002
UniProtKB: Q9BX673
Ensembl: ENSG000001660867
Search outside databases for aliases for JAM3 gene

Previous GC identifers: GC11P136435 GC11P135453 GC11P133972 GC11P133476

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for JAM3:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell
sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes
and water from passing freely through the paracellular space. The protein encoded by this
immunoglobulin superfamily gene member is localized in the tight junctions between high
endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to
leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member
of the junctional adhesion molecule protein family and acts as a receptor for another member of
this family. [provided by RefSeq]

UniProtKB/Swiss-Prot: JAM3_HUMAN, Q9BX67
Function: May participate in cell-cell adhesion distinct from tight junctions

Gene Wiki entry for JAM3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the JAM3 gene  

Entrez Gene cytogenetic band: 11q25   Ensembl cytogenetic band:  11q25   HGNC cytogenetic band: 11q25

JAM3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P133444:     (about GC identifiers)

Start:
133,444,030 bp from pter
End:
133,526,861 bp from pter
Size:
82,832 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000011.8  NT_033899.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: JAM3_HUMAN, Q9BX67 (See protein sequence)
Recommended Name: Junctional adhesion molecule C precursor  
Size: 310 amino acids; 35020 Da
Subunit: Interacts with JAM2
Subcellular location: Cell membrane; Single-pass type I membrane protein (Potential)
Secondary accessions: Q8WWL8 Q96FL1

REFSEQ proteins: NP_116190.2  

ENSEMBL proteins: 
ENSP00000299106 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
R&D Systems Recombinant & Natural Proteins for JAM3 (JAM-C)
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Junctional Adhesion Molecule C)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for JAM3: 
Invitrogen Antibodies for JAM3
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for JAM3
R&D Systems Antibodies for JAM3 (JAM-C)
Antibodies from Abcam (Junctional Adhesion Molecule C), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (JAM3)
Novus Biologicals Antibody for JAM3

Assays for JAM3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/6 InterPro domains/families (see all 6 ):
 IPR003598 Ig_sub2
 IPR007110 Ig-like
 IPR003599 Ig_sub
 IPR013783 Ig-like_fold
 IPR013106 Ig_V-set


   GeneDecks  JAM3 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9BX67

ProtoNet protein and cluster: Q9BX67

2 Blocks protein families:
IPB003598 Immunoglobulin C-2 type
IPB013106 Immunoglobulin V-set


UniProtKB/Swiss-Prot: JAM3_HUMAN, Q9BX67
Similarity: Belongs to the immunoglobulin superfamily
Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (JAM3)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (JAM3)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032801

              Applied Biosystems Silencer® siRNAs for JAM3

              Sigma-Aldrich siRNA and siRNA Panels for JAM3  
                     Sigma-Aldrich shRNA for JAM3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for JAM3
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_032801
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_032801
                                 untagged cDNA clone in CMV expression vector: NM_032801 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_032801

UniProtKB/Swiss-Prot: JAM3_HUMAN, Q9BX67
Function: May participate in cell-cell adhesion distinct from tight junctions

8 MGI mutant phenotypes (inferred from 1 allele(MGI details for Jam3):

digestive/alimentaryendocrine/exocrine glandhematopoietic systemimmune systemlethality-postnatal
life span-post-weaning/agingreproductive systemrespiratory system

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Millipore Pathway for JAM3
 Cell adhesion Tight junctions

   GeneDecks  JAM3 for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  JAM3  (Your Favorite Gene powered by Ingenuity) 
 Tight Junction Signaling
 Leukocyte Extravasation Signaling

   GeneDecks  JAM3 for the pathways selected above  
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4 Kegg Pathways  (Kegg details for JAM3):
 hsa04514 Cell adhesion molecules (CAMs)
 hsa04530 Tight junction
 hsa04670 Leukocyte transendothelial migration
 hsa05120 Epithelial cell signaling in Helicobacter pylori infection

   GeneDecks  JAM3 for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  JAM3 


5/37 Interacting proteins for JAM3 (ENSP000002991063 Q9BX672) via UniProtKB, MINT, and/or STRING (see all 37 )
InteractantInteraction Details
GeneCardExternal ID(s)
PARD3Q8TEW02STRING (score=.964) MINT-4085406
ITGB2ENSP000003472793STRING (score=.99)
TJP1ENSP000002815373STRING (score=.984)
ITGAMENSP000002874973STRING (score=.981)
ITGA4ENSP000002335733STRING (score=.977)
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for JAM3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (JAM3)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (JAM3)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032801

              Sigma-Aldrich siRNA and siRNA Panels for JAM3  
                     Sigma-Aldrich shRNA for JAM3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_032801  

REFSEQ mRNAs for JAM3 gene: 

NM_032801.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_032801  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_032801
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_032801
                                 untagged cDNA clone in CMV expression vector: NM_032801 

Additional cDNA sequence: 

AF356518.1 AF448478.1 AJ344431.1 AJ416101.1 AK027435.1 AK074769.1 AK075309.1 AK125071.1 
AK225666.1 AY358335.1 BC010690.1 BC012147.1 BC057284.1 BC063031.1 CR601575.1 CR609180.1 
CR612469.1 CR617412.1 CR624885.1 

18 DOTS entries:

DT.442715  DT.100025623  DT.95191672  DT.97799659  DT.97769862  DT.120684377  DT.101983753  DT.100020795 
DT.70105331  DT.100852217  DT.120684383  DT.100684258  DT.100864300  DT.120684251  DT.120684342  DT.92044977 
DT.95191809  DT.95375209 

24/220 AceView cDNA sequences (see all 220 ):

BC010690 BE464631 CR624885 CD172465 AW953057 AW026671 T32676 AW162934 
AI351676 AI089282 BU735616 CR612469 AA149513 AI131505 AA931102 BQ439580 
CR609180 AU280703 AW051295 AW005213 BE909801 CA412385 AW262622 BQ026358 

highest scoring ESTs for JAM3:

AA149644 AA306190 AA029134 AA043327 AA043328 AA082108 AA131882 AA131883 AA149513 AA404374 

Unigene Cluster for JAM3:

Junctional adhesion molecule 3
Hs.150718  [show with all ESTs]
Unigene Representative Sequence: NM_032801


GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for JAM3

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c
SP1:                                      -           -                                 
SP2:                          -           -           -                                 
SP3:              -     -     -     -     -     -     -     -                           
SP4:                                                                                    
SP5:                                                                                    

About this scheme

ECgene alternative splicing isoforms for JAM3

1 Ensembl transcript including schematic representation:
ENST00000299106  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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JAM3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for JAM3

1 / 2 / 3

12 probe-sets matching JAM3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
81761_at2, 3 U95-E 1 1.00 1.00 0.98 1.31 H42085 0.80 1.00 0.91 1
60446_at2, 3 U95-C 1 1.00 1.00 0.93 1.26 AA306190 1.00 1.00 1.00 1
32526_at2, 3 U95-A 1 1.00 1.00 0.97 0.86 AA149644 1.00 1.00 1.00 1

71532_at2, 3 U95-E 1 0.44 1.00 0.81 0.48 AI937029 0.60 1.00 0.82 1

32525_r_at*2, 3 U95-A 1 -- -- -0.37 1.14 W29012 0.40 0.67 0.55 1

32524_s_at*2, 3 U95-A 1 -- -- -0.30 0.22 W29012 0.40 0.67 0.55 1

231721_at2, 3 U133-B 1 1.00 1.00 -- -- AF356518 0.80 1.00 0.91 1

231720_s_at2, 3 U133-B 1 1.00 1.00 -- -- AF356518 0.80 1.00 0.91 1

212813_at2, 3 U133-A 1 1.00 1.00 -- -- AA149644 1.00 1.00 1.00 1

231721_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

231720_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

212813_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  JAM3 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.150718

Expression variation in blood from EXPOLDB for JAM3

UniProtKB/Swiss-Prot: JAM3_HUMAN, Q9BX67
Tissue specificity: Widely expressed. Highest expression in placenta, brain and kidney

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for JAM3 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
JAM31   -- junctional adhesion molecule 3 85.12(n)
87.8(a)
489271  XM_546389.2  XP_546389.2 
chimpanzee
(Pan troglodytes)
JAM31   -- junctional adhesion molecule 3 99.19(n)
99.31(a)
451678  XM_529458.2  XP_529458.2 
cow
(Bos taurus)
JAM31   -- junctional adhesion molecule 3 81.46(n)
88.75(a)
513412  XM_591084.3  XP_591084.3 
rat
(Rattus norvegicus)
Jam31   -- junctional adhesion molecule 3 84.62(n)
85.48(a)
315509  NM_001004269.1  NP_001004269.1 
mouse
(Mus musculus)
Jam31, 5 95
junction adhesion molecule 31, 5 85.59(n)1
86.45(a)1
839641  NM_023277.21  NP_075766.11 
 AA6671545  AI8502975  (see all 14)
About this table        Species with no ortholog for JAM3

ENSEMBL Gene Tree for JAM3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for JAM3 gene
F11R2  JAM22  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/601 NCBI SNPs in JAM3 are shown (see all 601 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 186)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 11 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs6434471,2
A,C,F,H133526687(+) AAAAGG/TAATAC 1 -- ut31 trp38Minor allele frequency- T:0.18MN EU EA WA NA 744
rs118226251,2
A,C,F,H133527008(+) TGCTCA/GAGAGC 1 -- ng314Minor allele frequency- G:0.04EU EA WA 418
rs6410241,2
A,C,F,H133526058(+) CATCCG/ACCGGA 1 -- ut31 ese316Minor allele frequency- A:0.21MN EU EA WA NA 1582
rs112237161,2
A,C,F,H133525432(-) GGTACA/CGACTG 1 -- ut3111Minor allele frequency- C:0.28EU EA WA NA 1112
rs79364211,2
A,C,F,H133525111(+) TACAAT/CGTTAA 1 -- ut314Minor allele frequency- C:0.11EU EA WA 408
rs5973201,2
A,C,F,H133524571(+) CATGAA/GTATTA 1 -- ut31 ese312Minor allele frequency- G:0.49MN NA EU EA WA 1166
rs122890841,2
C,F,H133525827(+) AAGTAC/TAATAA 1 -- ut31 ese38Minor allele frequency- T:0.06EU EA WA 840
rs38249941,2
F,H133526061(+) CCGCCG/AGAGAC 1 -- ut31 ese34Minor allele frequency- A:0.01EU EA WA 412
rs112237151,2
F,H133524885(+) TAAATT/CGGTTG 1 -- ut314Minor allele frequency- C:0.09EU EA WA 420
--
rs20025271,2
C133442041(-) aaataC/Gaaaaa 1 -- ng510--------
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HapMap Linkage Disequilibrium images for JAM3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 606871

Human Genome Epidemiology Navigator: JAM3 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/37 PubMed articles for JAM3 gene (see all 37 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 83700 HGNC: 15532 AceView: JAM3 Ensembl:ENSG00000166086 euGenes: HUgn83700
ECgene: JAM3 H-InvDB: JAM3
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for JAM3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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 Predesigned and custom siRNAs for JAM3 Antibodies for JAM3
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for JAM3 Browse small molecules at Sigma-Aldrich
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 Antibodies for JAM3

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