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JAM2 Gene

protein-coding   GIFtS: 57

GC21P025933
junctional adhesion molecule 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: C21orf43)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
C21orf43 2, 3
CD322 1, 2
JAM-B 1, 2, 3
JAM-IT/VE-JAM 2
JAMB 1, 2
OTTHUMP00000096100 2
PRO245 2
VE-JAM 1, 2, 3
VEJAM 2, 3, 5
Descriptions
CD322 antigen 3
Vascular endothelial junction-associated molecule 2, 3
junctional adhesion molecule 2 2, 3
junctional adhesion molecule B 2
External Ids
HGNC: 146861
Entrez Gene: 584942
UniProtKB: P570873
Ensembl: ENSG000001547217
Search outside databases for aliases for JAM2 gene

Previous GC identifers: GC21P023590 GC21P023673

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for JAM2:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell
sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes
and water from passing freely through the paracellular space. The protein encoded by this
immunoglobulin superfamily gene member is localized in the tight junctions between high
endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell
types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq]

UniProtKB/Swiss-Prot: JAM2_HUMAN, P57087
Function: May play a role in the processes of lymphocyte homing to secondary lymphoid organs

Gene Wiki entry for JAM2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 21q21.2   Ensembl cytogenetic band:  21q21.3   HGNC cytogenetic band: 21q21.2

JAM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 21         GeneLoc Exon Structure

GeneLoc location for GC21P025933:     (about GC identifiers)

Start:
25,933,455 bp from pter
End:
26,011,745 bp from pter
Size:
78,291 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000021.7  NT_011512.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: JAM2_HUMAN, P57087 (See protein sequence)
Recommended Name: Junctional adhesion molecule B precursor  
Size: 298 amino acids; 33207 Da
Subunit: Interacts with JAM3
Subcellular location: Cell junction, tight junction (By similarity). Cell membrane; Single-pass
type I membrane protein (By similarity). Note=Localized at tight junctions of both epithelial and
endothelial cells (By similarity)
Secondary accessions: B2R6T9 Q6UXG6 Q6YNC1

REFSEQ proteins: NP_067042.1  

ENSEMBL proteins: 
ENSP00000383376 ENSP00000383377 ENSP00000383381 ENSP00000383379 ENSP00000318416 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
R&D Systems Recombinant & Natural Proteins for JAM2 (JAM-B/VE-JAM)
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Junctional Adhesion Molecule 2)
Human Recombinant Proteins from Abnova (JAM2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane IEA--
GO:0005887 integral to plasma membrane NAS10779521
GO:0005923 tight junction IEA--
GO:0030054 cell junction IEA--
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Antibodies for JAM2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for JAM2
R&D Systems Antibodies for JAM2 (JAM-B/VE-JAM)
Antibodies from Abcam (Junctional Adhesion Molecule 2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (JAM2)
Novus Biologicals Antibodies for JAM2

Assays for JAM2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/6 InterPro domains/families (see all 6 ):
 IPR003598 Ig_sub2
 IPR007110 Ig-like
 IPR003599 Ig_sub
 IPR013783 Ig-like_fold
 IPR013106 Ig_V-set


   GeneDecks  JAM2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P57087

ProtoNet protein and cluster: P57087

2 Blocks protein families:
IPB003598 Immunoglobulin C-2 type
IPB013106 Immunoglobulin V-set


UniProtKB/Swiss-Prot: JAM2_HUMAN, P57087
Similarity: Belongs to the immunoglobulin superfamily
Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (JAM2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (JAM2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021219

              Applied Biosystems Silencer® siRNAs for JAM2

              Sigma-Aldrich siRNA and siRNA Panels for JAM2  
                     Sigma-Aldrich shRNA Panels and shRNA for JAM2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for JAM2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021219
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021219
                                 untagged cDNA clone in CMV expression vector: NM_021219 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_021219

UniProtKB/Swiss-Prot: JAM2_HUMAN, P57087
Function: May play a role in the processes of lymphocyte homing to secondary lymphoid organs

1 MGI mutant phenotype (inferred from 1 allele(MGI details for Jam2):

normal

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Millipore Pathways for JAM2
 Cell adhesion Tight junctions
 Cell adhesion Endothelial cell contacts by junctional mechanisms

   GeneDecks  JAM2 for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  JAM2  (Your Favorite Gene powered by Ingenuity) 
 Tight Junction Signaling
 Leukocyte Extravasation Signaling

   GeneDecks  JAM2 for the pathways selected above  
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4 Kegg Pathways  (Kegg details for JAM2):
 hsa04514 Cell adhesion molecules (CAMs)
 hsa04530 Tight junction
 hsa04670 Leukocyte transendothelial migration
 hsa05120 Epithelial cell signaling in Helicobacter pylori infection

   GeneDecks  JAM2 for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  JAM2 


5/31 Interacting proteins for JAM2 (ENSP000003184163 P570872) via UniProtKB, MINT, and/or STRING (see all 31 )
InteractantInteraction Details
GeneCardExternal ID(s)
PARD3Q8TEW02STRING (score=.964) MINT-4085389
ITGA4ENSP000002335733STRING (score=.977)
TJP1ENSP000002815373STRING (score=.972)
JAM3ENSP000002991063STRING (score=.965)
ITGB1ENSP000003640943STRING (score=.953)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016337 cell-cell adhesion NAS10779521
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for JAM2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (JAM2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (JAM2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021219

              Sigma-Aldrich siRNA and siRNA Panels for JAM2  
                     Sigma-Aldrich shRNA Panels and shRNA for JAM2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_021219  

REFSEQ mRNAs for JAM2 gene: 

NM_021219.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_021219  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021219
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021219
                                 untagged cDNA clone in CMV expression vector: NM_021219 

Additional cDNA sequence: 

AF255910.1 AK294769.1 AK312708.1 AY016009.1 AY077698.1 AY358361.1 BC017779.1 CR599894.1 
CR605305.1 CR610497.1 CR612856.1 CR618843.1 CR621227.1 CR621411.1 

10 DOTS entries:

DT.411907  DT.100801616  DT.411908  DT.100784546  DT.100835567  DT.416224  DT.121138111  DT.99982500 
DT.121138107  DT.86845463 

24/137 AceView cDNA sequences (see all 137 ):

AY016009 CR599894 BM955588 AY358361 CB125035 CB138920 BM723158 CR621411 
AA865038 CR610497 BU730372 AA405018 CD172203 AI652914 AY077698 AI421450 
CR621227 CR618843 F04244 BM955591 AI201753 BM921497 NM_021219 AA405134 

highest scoring ESTs for JAM2:

AA725566 AA410345 AA865038 AA912674 AA987434 AI017553 AI052637 AI123727 AI140139 AI201753 

Unigene Cluster for JAM2:

Junctional adhesion molecule 2
Hs.517227  [show with all ESTs]
Unigene Representative Sequence: NM_021219


GeneLoc Exon Structure

5 Ensembl transcripts including schematic representations:
ENST00000400532  ENST00000400533  ENST00000400537  ENST00000400535  ENST00000312957  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

JAM2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for JAM2

1 / 2 / 3

7 probe-sets matching JAM2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

62201_r_at2, 3 U95-C 1 1.00 1.00 0.93 0.70 AA725566 1.00 1.00 1.00 1
45377_s_at2, 3 U95-B 1 1.00 1.00 0.98 1.16 AI199779 0.60 1.00 0.82 1
64913_at2, 3 U95-C 1 0.81 1.00 0.97 1.16 R51371 0.20 0.50 0.38 1

219213_at2, 3 U133-A 1 1.00 1.00 -- -- NM_021219 0.60 1.00 0.82 1

229127_at2, 3 U133-B 1 1.00 1.00 -- -- BF195118 0.20 0.50 0.38 1

219213_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

229127_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  JAM2 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGCCTGCAGT

SOURCE GeneReport for Unigene cluster: Hs.517227

UniProtKB/Swiss-Prot: JAM2_HUMAN, P57087
Tissue specificity: Highest expression in the heart, placenta, lung, foreskin and lymph node.
Prominently expressed on high endothelial venules, also present on the endothelia of other
vessels. Localized to the intercellular boundaries of high endothelial cells

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for JAM2 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
JAM21   -- junctional adhesion molecule 2 91.16(n)
93.29(a)
478392  XM_535568.2  XP_535568.1 
chimpanzee
(Pan troglodytes)
JAM21   -- junctional adhesion molecule 2 99.89(n)
100(a)
458486  XM_514843.2  XP_514843.2 
cow
(Bos taurus)
JAM21   -- junctional adhesion molecule 2 90.6(n)
90.94(a)
538846  XM_583230.3  XP_583230.3 
rat
(Rattus norvegicus)
Jam21   -- junctional adhesion molecule 2 83.05(n)
81.14(a)
619374  NM_001034004.1  NP_001029176.1 
mouse
(Mus musculus)
Jam21, 5 165
junction adhesion molecule 21, 5 82.6(n)1
79.46(a)1
673741  NM_023844.31  NP_076333.21 
 AF2559115  AI8537245  (see all 18)
About this table        Species with no ortholog for JAM2

ENSEMBL Gene Tree for JAM2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for JAM2 gene
JAM32  F11R2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/461 NCBI SNPs in JAM2 are shown (see all 461 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 128)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 21 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs28298791,2
A,C,F,H,O26009161(+) TAAAAT/CATAAC 1 -- ng3110Minor allele frequency- C:0.28NA EU EA WA 760
rs28298771,2
A,C,F,H,O26008915(+) TATTAT/CAAAAC 1 -- ut318Minor allele frequency- C:0.06NA EU EA WA 580
rs7633321,2
C,F,H,O25931649(+) CCACAG/ATTCTA 1 -- ng5117Minor allele frequency- A:0.26NS NA EU EA WA 1102
rs9746801,2
A,C,F,H25932983(+) GTCCAA/GTGTAA 1 -- ng515Minor allele frequency- G:0.39MN EU EA WA 604
rs28298781,2
C,F,H,O26009036(+) TCTGTC/TGACAC 1 -- ut31 ese312Minor allele frequency- T:0.08NA EU EA WA 998
rs96471121,2
C,F,H25931913(+) TATGTC/GCTTCC 1 -- ng51 trp38Minor allele frequency- G:0.16EU EA WA 822
rs99763821,2
C,F,H26006557(+) CAATGA/CGTGAA 1 S/R mis18Minor allele frequency- C:0.02EU EA WA 840
rs117024201,2
C,H26008953(+) GTACAC/TGAGGA 1 -- ut31 ese34Minor allele frequency- T:0.00EU EA WA 418
rs48170451,2
C25931989(+) GAACAG/TCCCCT 1 -- ng510--------
rs24091431,2
C25932607(+) CTGGGC/TCCCCT 1 -- ng510--------
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HapMap Linkage Disequilibrium images for JAM2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 606870

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/23 PubMed articles for JAM2 gene (see all 23 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 58494 HGNC: 14686 AceView: JAM2 Ensembl:ENSG00000154721 euGenes: HUgn58494
ECgene: JAM2 H-InvDB: JAM2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for JAM2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for JAM2 Antibodies for JAM2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for JAM2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Antibodies (Junctional Adhesion Molecule 2)
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 Antibodies for JAM2

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