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ITK Gene

protein-coding   GIFtS: 71
GCID: GC05P156607

IL2-Inducible T-Cell Kinase

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
IL2-Inducible T-Cell Kinase1 2     EC 2.7.10.23 8
EMT2 3 5     LPFS12 5
Interleukin-2-Inducible T-Cell Kinase2 3     PSCTK22
Kinase EMT2 3     Homolog Of Mouse T-Cell Itk/Tsk2
Tyrosine-Protein Kinase Lyk2 3     IL-2-Inducible T Cell Kinase2
LYK2 3     Interleukin-2-Inducible T Cell Kinase2
IL-2-Inducible T-Cell Kinase2 3     Tyrosine-Protein Kinase ITK/TSK2
T-Cell-Specific Kinase2 3     EC 2.7.108

External Ids:    HGNC: 61711   Entrez Gene: 37022   Ensembl: ENSG000001132637   OMIM: 1869735   UniProtKB: Q088813   

Export aliases for ITK gene to outside databases

Previous GC identifers: GC05P156656 GC05P157158 GC05P156543 GC05P156588 GC05P156540 GC05P151695


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for ITK Gene:
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3
domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and
differentiation. (provided by RefSeq, Jul 2008)

GeneCards Summary for ITK Gene:
ITK (IL2-inducible T-cell kinase) is a protein-coding gene. Diseases associated with ITK include lymphoproliferative syndrome 1, and lymphoproliferative syndrome, ebv-associated, autosomal, 1. GO annotations related to this gene include phospholipid binding and non-membrane spanning protein tyrosine kinase activity. An important paralog of this gene is TEC.

UniProtKB/Swiss-Prot: ITK_HUMAN, Q08881
Function: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates
the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen
presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK
to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK.
Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1,
leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic
reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates
into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for
activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are
recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation

Gene Wiki entry for ITK Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000005.10  NC_018916.2  NT_023133.14  
Regulatory elements:
   Regulatory transcription factor binding sites in the ITK gene promoter:
         STAT1   AML1a   GATA-3   FOXJ2 (long isoform)   c-Myb   Nkx3-1 v1   FOXJ2   IRF-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidITK promoter sequence
   Search Chromatin IP Primers for ITK

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ITK


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q31-q32   Ensembl cytogenetic band:  5q33.3   HGNC cytogenetic band: 5q31-q32

ITK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ITK gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P156607:  view genomic region     (about GC identifiers)

Start:
156,569,944 bp from pter      End:
156,682,201 bp from pter
Size:
112,258 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ITK_HUMAN, Q08881 (See protein sequence)
Recommended Name: Tyrosine-protein kinase ITK/TSK  
Size: 620 amino acids; 71831 Da
Cofactor: Binds 1 zinc ion per subunit (By similarity)
Subunit: Homooligomerizes; this association negatively regulates kinase activity (By similarity). Interacts with
PPIA/CYPA; this interaction regulates TCR signal strength via a proline-directed conformational switch in ITK.
Interacts with THEMIS (By similarity). Interacts with FASLG. Interacts with VAV1; this interaction is important
for VAV1 localization and TCR-induced actin polarization
Selected PDB 3D structures from and Proteopedia for ITK (see all 23):
1SM2 (3D)        1SNU (3D)        1SNX (3D)        2E6I (3D)        2LMJ (3D)        2YUQ (3D)    
Secondary accessions: B2R752 Q32ML7

Explore the universe of human proteins at neXtProt for ITK: NX_Q08881

Explore proteomics data for ITK at MOPED

Post-translational modifications: 

  • Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at
    Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence
    of the SH3 domain1
  • Ubiquitinated1
  • Ubiquitination2 at Lys119, Lys129, Lys138, Lys150, Lys242, Lys258, Lys280, Lys291, Lys310, Lys344,
                                 Lys412, Lys497, Lys519, Lys523
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for ITK (Q08881) (see all 6)
     RDLAARN  KSDVWSFG  LVQLYGVC  KKPLPPTPE 


    See ITK Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_005537.3  
    ENSEMBL proteins: 
     ENSP00000430327   ENSP00000398655   ENSP00000431054  
    Reactome Protein details: Q08881

    ITK Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant ITK Protein
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    OriGene Custom Protein Services for ITK
    GenScript Custom Purified and Recombinant Proteins Services for ITK
    Novus Biologicals ITK Proteins
    Novus Biologicals ITK Lysates
    Sino Biological Recombinant Protein for ITK
    Sino Biological Cell Lysate for ITK
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for ITK

    ITK Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of ITK
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    OriGene Custom Antibody Services for ITK
    Novus Biologicals ITK Antibodies
    Abcam antibodies for ITK
    Cloud-Clone Corp. Antibodies for ITK
    ThermoFisher Antibody for ITK
    LSBio Antibodies in human, mouse, rat for ITK

    ITK Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ITK
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ITK
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ITK
    Cloud-Clone Corp. CLIAs for ITK


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    PLEKH: Pleckstrin homology (PH) domain containing
    SH2D: SH2 domain containing

    IUPHAR Guide to PHARMACOLOGY protein family classification: IL2-inducible T-cell kinase
    Tec family

    Selected InterPro protein domains (see all 11):
     IPR017441 Protein_kinase_ATP_BS
     IPR011009 Kinase-like_dom
     IPR001849 Pleckstrin_homology
     IPR001452 SH3_domain
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry Q08881

    ProtoNet protein and cluster: Q08881

    5 Blocks protein domains:
    IPB000980 SH2 domain signature
    IPB001452 SH3 domain signature
    IPB001562 Tec/Btk domain signature
    IPB001849 Pleckstrin-like
    IPB008266 Tyrosine protein kinase


    UniProtKB/Swiss-Prot: ITK_HUMAN, Q08881
    Domain: The N-terminal PH domain allows ITK to be recruited to the plasma membrane by an activated PI3 kinase.
    This domain contains also a proline-rich region (PRR). The adjoining domain is a SH3 domain, which binds to PRR
    (from itself or from other proteins). Next, a SH2 domain is required for binding tyrosine-phosphorylated
    substrates. In the C-terminal region, the kinase domain is required for tyrosine phosphorylation
    Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily
    Similarity: Contains 1 Btk-type zinc finger
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 SH2 domain
    Similarity: Contains 1 SH3 domain


    ITK for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ITK_HUMAN, Q08881
    Function: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates
    the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen
    presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK
    to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK.
    Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1,
    leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic
    reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates
    into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for
    activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are
    recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation
    Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
    Induction: Through a myriad of surface receptors including the TCR/CD3 signaling complex, coreceptors, or
    chemokine receptors

         Genatlas biochemistry entry for ITK:
    tyrosine kinase,72kDa,mainly expressed in T cells and natural killer(NK) cells,SRC family,Tec subfamily

         Enzyme Numbers (IUBMB): EC 2.7.10.21 2 EC 2.7.102

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004713protein tyrosine kinase activity ----
    GO:0004715non-membrane spanning protein tyrosine kinase activity IDA12186560
    GO:0005515protein binding IPI12186560
    GO:0005524ATP binding IEA--
         
    ITK for ontologies           About GeneDecksing


    Phenotypes:
         5 GenomeRNAi human phenotypes for ITK:
     Decreased 12E8 tau but not tot  Decreased focal adhesion (FA)   Decreased viability in CMK cel  Increased HPV18 LCR reporter a 
     Large cells 

         3 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Itk):
     cellular  hematopoietic system  immune system 

    ITK for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for ITK: Itktm1Litt Itktm1Ljb

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ITK
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ITK

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ITK
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ITK

    miRNA
    Products:
        
    Block miRNA regulation of human, mouse, rat ITK using miScript Target Protectors
    8 qRT-PCR Assays for microRNAs that regulate ITK:
    hsa-miR-140-3p hsa-miR-513b hsa-miR-127-5p hsa-miR-155 hsa-miR-943 hsa-miR-519c-3p hsa-miR-519a hsa-miR-519b-3p
    SwitchGear 3'UTR luciferase reporter plasmidITK 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ITK
    Predesigned siRNA for gene silencing in human, mouse, rat ITK

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ITK

    Clone
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    OriGene clones in human, mouse for ITK (see all 8)
    OriGene ORF clones in mouse, rat for ITK
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ITK (NM_005546)
    Sino Biological Human cDNA Clone for ITK
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ITK
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ITK

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for ITK
    Browse ESI BIO Cell Lines and PureStem Progenitors for ITK 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ITK


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ITK_HUMAN, Q08881: Cytoplasm. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane
    after receptor stimulation
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol4
    nucleus2
    plasma membrane2
    cytoskeleton1

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829cytosol TAS--

    ITK for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ITK About   (see all 23)  
    See pathways by source

    SuperPathContained pathways About
    1B cell receptor signaling pathway (KEGG)
    T cell receptor signaling pathway0.38
    TCR Signaling Pathway0.36
    2Immune response NFAT in immune response
    Immune response NFAT in immune response0.55
    Immune response T cell receptor signaling pathway0.50
    Immune response ICOS pathway in T helper cell0.55
    Immune response CD28 signaling0.49
    3ICos-ICosL Pathway in T-Helper Cell
    ICos-ICosL Pathway in T-Helper Cell0.66
    CD28 Signaling in T-Helper Cell0.66
    4TCR Signaling (Qiagen)
    TCR Signaling0.68
    ITK and TCR Signaling0.54
    5TCR signaling (REACTOME)
    TCR signaling0.73

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for ITK (see all 10)
        ICos-ICosL Pathway in T-Helper Cell
    CDC42 Pathway
    CD28 Signaling in T-Helper Cell
    Tec Kinases Signaling
    Transendothelial Migration of Leukocytes

    2 Cell Signaling Technology (CST) Pathways for ITK
        Lymphocyte Signaling
    Tyrosine Kinases / Adaptors

    4 GeneGo (Thomson Reuters) Pathways for ITK
        Immune response ICOS pathway in T-helper cell
    Immune response T cell receptor signaling pathway
    Immune response CD28 signaling
    Immune response NFAT in immune response

    5 BioSystems Pathways for ITK
        B Cell Receptor Signaling Pathway
    TCR Signaling Pathway
    TCR signaling in naive CD4+ T cells
    Class I PI3K signaling events
    Fc-epsilon receptor I signaling in mast cells


    2 Reactome Pathways for ITK
        FCERI mediated Ca+2 mobilization
    Generation of second messenger molecules


    3 Kegg Pathways  (Kegg details for ITK):
        Chemokine signaling pathway
    T cell receptor signaling pathway
    Leukocyte transendothelial migration


    ITK for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ITK
    Interactions:

        GeneGlobe Interaction Network for ITK

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ITK (Q088811, 2, 3 ENSP000003986554) via UniProtKB, MINT, STRING, and/or I2D (see all 78)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ERBB2P046261, 2, 3, ENSP000002695714EBI-968552,EBI-641062 MINT-74558 MINT-74557 I2D: score=2 STRING: ENSP00000269571
    LCP2Q130942, 3, ENSP000000467944MINT-59473 MINT-8224277 I2D: score=6 STRING: ENSP00000046794
    PLCG1P191742, 3, ENSP000002440074MINT-8224277 I2D: score=3 STRING: ENSP00000244007
    LCKP062392, 3, ENSP000003378254MINT-8224277 I2D: score=2 STRING: ENSP00000337825
    ABI1Q8IZP02, 3, ENSP000003653124MINT-7908362 I2D: score=1 STRING: ENSP00000365312
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 15):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001816cytokine production ISS--
    GO:0001865NK T cell differentiation IEA--
    GO:0002250adaptive immune response ISS--
    GO:0006468protein phosphorylation ----
    GO:0006968cellular defense response TAS8364206

    ITK for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ITK

    2 HMDB Compounds for ITK    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--

    2 DrugBank Compounds for ITK    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Staurosporine-- 62996-74-1target--17139284 17016423
    PazopanibGW-786034 (see all 2)444731-52-6targetinhibitor23072642

    Selected Novoseek inferred chemical compound relationships for ITK gene (see all 11)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 78 137 8609388 (4), 7561053 (4), 9130632 (3), 12511421 (3) (see all 70)
    phosphatidylinositol 49.2 13 9326591 (2), 17060314 (2), 10636929 (1), 10958690 (1) (see all 6)
    phosphotyrosine 38.5 4 19955438 (2)
    threonine 37.3 2 11282020 (1), 7561053 (1)
    benzimidazole 33.8 2 18819799 (1), 18819794 (1), 17499505 (1)
    proline 28.9 5 12163161 (3), 15574377 (1)
    serine 22.4 2 11282020 (1), 7561053 (1)
    calcium 22.2 14 18181691 (3), 19917685 (2), 10636929 (1), 11313393 (1) (see all 8)
    phenylalanine 14.8 2 17897671 (1), 9312162 (1)
    lipid 0 4 9326591 (1), 11709089 (1)



    ITK for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for ITK gene: 
    NM_005546.3  

    Unigene Cluster for ITK:

    IL2-inducible T-cell kinase
    Hs.558348  [show with all ESTs]
    Unigene Representative Sequence: AB209622
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000521769 ENST00000422843(uc003lwo.1) ENST00000522616 ENST00000520555
    ENST00000519402 ENST00000517779 ENST00000519759 ENST00000520173 ENST00000519749
    ENST00000523926
    miRNA
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    8 qRT-PCR Assays for microRNAs that regulate ITK:
    hsa-miR-140-3p hsa-miR-513b hsa-miR-127-5p hsa-miR-155 hsa-miR-943 hsa-miR-519c-3p hsa-miR-519a hsa-miR-519b-3p
    SwitchGear 3'UTR luciferase reporter plasmidITK 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ITK
    Clone
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    GenScript: all cDNA clones in your preferred vector: ITK (NM_005546)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ITK
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ITK
    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ITK
      QuantiTect SYBR Green Assays in human, mouse, rat ITK
      QuantiFast Probe-based Assays in human, mouse, rat ITK

    Additional mRNA sequence: 

    AB209622.1 AK303609.1 AK311654.1 AK312846.1 BC094701.1 BC109077.2 BC109078.1 BX538196.1 
    D13720.1 

    7 DOTS entries:

    DT.95168684  DT.100702730  DT.101979383  DT.75110023  DT.100707524  DT.101968004  DT.40291503 

    Selected AceView cDNA sequences (see all 36):

    AU076776 AA354109 BX538196 NM_005546 AW024320 D13720 BI835577 AI279520 
    CB117873 CD641711 AA348218 CD638420 CD640822 AI803120 AW439677 CD639056 
    BX646272 AA309795 AI862176 R00569 BV208133 BI911371 T29796 CD639621 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ITK (see all 10)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c ^ 13a · 13b · 13c ^ 14a · 14b ^ 15a · 15b · 15c ^
    SP1:                    -                 -     -                 -                                               -                                             
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                        -   
    SP4:                                                                                                                                                            
    SP5:                    -                 -                                                                                                                     

    ExUns: 16a · 16b ^ 17a · 17b ^ 18a · 18b ^ 19a · 19b
    SP1:  -                                             
    SP2:                                                
    SP3:  -                                             
    SP4:  -     -                                       
    SP5:                                                


    ECgene alternative splicing isoforms for ITK

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ITK expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    ITK Expression
    About this image


    ITK expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Blood (Cardiovascular System)    fully expand to see all 2 entries
             T Helper Cells Peripheral Blood
     
     Thymus (Hematopoietic System)
             Double Negative 2 Thymocytes Thymus
    ITK Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ITK Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.558348

    UniProtKB/Swiss-Prot: ITK_HUMAN, Q08881
    Tissue specificity: T-cell lines and natural killer cell lines

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for ITK gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Itk1 , 5 IL2 inducible T cell kinase1, 5 88.05(n)1
    94.35(a)1
      11 (27.75 cM)5
    164281  NM_001281965.11  NP_001268894.11 
     463251505 
    chicken
    (Gallus gallus)
    Aves ITK1 IL2-inducible T-cell kinase 74.66(n)
    78.12(a)
      416247  XM_414568.3  XP_414568.3 
    lizard
    (Anolis carolinensis)
    Reptilia ITK6
    IL2-inducible T-cell kinase
    74(a)
    1 ↔ 1
    GL343205.1(2576217-2621650)
    zebrafish
    (Danio rerio)
    Actinopterygii itk2 IL2-inducible T-cell kinase 77.18(n)   30323  AF016326.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Btk29A3 male genital morphogenesis (sensu
    Holometabola) more
    50(a)   29A1   --


    ENSEMBL Gene Tree for ITK (if available)
    TreeFam Gene Tree for ITK (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ITK gene
    TEC2  CSK2  FER2  TXK2  FES2  DSTYK2  MATK2  BMX2  
    BTK2  
    Selected SIMAP similar genes for ITK using alignment to 3 protein entries:     ITK_HUMAN (see all proteins) (see all similar genes):
    TXK    BTK kinase deficient isoform 2    TEC    EPHA2    BMX    FYN
    BCR/ABL fusion    ABL1    BLK    FGR    FRK    HCK
    SRC    YES1    LCK    ABL2    LYN    BTK

    ITK for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ITK (see all 1760)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1219081911,2,,4
    CLymphoproliferative syndrome 1 (LPFS1)4 pathogenic1156893204(+) GACTCC/TGGTAT 2 R W mis10--------
    VAR_0417114
    A metastatic melanoma sample4--see VAR_0417112 P L mis40--------
    VAR_0417104
    A metastatic melanoma sample4--see VAR_0417102 R K mis40--------
    VAR_0417124
    A gastric adenocarcinoma sample4--see VAR_0417122 R Q mis40--------
    rs348930651,2
    C--156611156(+) TTTTTT/-CCAAG 1 -- int11Minor allele frequency- -:0.50NA 2
    rs595854251,2
    C--156612694(+) AAAAAA/-GCCAG 1 -- int11Minor allele frequency- -:0.00NA 2
    rs712850401,2
    C--156613693(-) CAAGGCT/-CTCTC 1 -- int11Minor allele frequency- -:0.50NA 2
    rs113817221,2
    C--156620886(+) TTTTT-/TAAAAA 1 -- int10--------
    rs1415430761,2
    C--156624635(+) CAAAA-/ATGGG 
            
    ATGAT
    1 -- int10--------
    rs563872631,2
    C--156624641(+) TGGGA-/TGATGC 1 -- int10--------

    HapMap Linkage Disequilibrium report for ITK (156569944 - 156682201 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for ITK: --
    Human Gene Mutation Database (HGMD): ITK
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ITK
    DNA2.0 Custom Variant and Variant Library Synthesis for ITK

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 186973   
    OMIM disorders: 613011  
    UniProtKB/Swiss-Prot: ITK_HUMAN, Q08881
  • Lymphoproliferative syndrome 1 (LPFS1) [MIM:613011]: A rare immunodeficiency characterized by extreme
    susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal
    outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia,
    recurrent infections. There is an increased risk for lymphoma. Note=The disease is caused by mutations affecting
    the gene represented in this entry

  • Selected diseases for ITK (see all 24):    
    About MalaCards
    lymphoproliferative syndrome 1    lymphoproliferative syndrome, ebv-associated, autosomal, 1    agammaglobulinemia    peripheral t-cell lymphoma
    pancytopenia    splenomegaly    allergic asthma    hemophagocytic lymphohistiocytosis
    protein s deficiency    wiskott-aldrich syndrome    gout    atopy
    asthma    allergic rhinitis    rhinitis    thrombocytopenia
    chronic myeloid leukemia    pneumonia    myeloid leukemia    influenza

    1 disease from the University of Copenhagen DISEASES database for ITK:
    Agammaglobulinemia

    ITK for disorders           About GeneDecksing

    6 Novoseek inferred disease relationships for ITK gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    x-linked agammaglobulinemia 81.4 2 10556826 (1), 8892607 (1)
    asthma allergic 58.4 8 16630934 (3), 12734350 (2), 19689375 (1), 20218931 (1)
    asthma 11.1 3 16159638 (1), 12734350 (1)
    pneumonia 0 2 15323564 (1), 12734350 (1)
    inflammation 0 7 19689375 (2), 16630934 (2), 15907913 (1), 20218931 (1)
    allergy 0 1 17069754 (1)

    Genetic Association Database (GAD): ITK
    Human Genome Epidemiology (HuGE) Navigator: ITK (5 documents)

    Export disorders for ITK gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ITK gene, integrated from 10 sources (see all 188):
    (articles sorted by number of sources associating them with ITK)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification, cloning, and characterization of a novel human T- cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. (PubMed id 8364206)1, 2, 3, 9 Gibson S....Mills G.B. (Blood 1993)
    2. Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity. (PubMed id 9312162)1, 2, 9 Heyeck S.D.... Berg L.J. (J. Biol. Chem. 1997)
    3. Tec kinase Itk forms membrane clusters specifically in the vicinity of recruiting receptors. (PubMed id 17060314)1, 2, 9 Qi Q.... August A. (J. Biol. Chem. 2006)
    4. A haplotype in the inducible T-cell tyrosine kinase is a risk factor for seasonal allergic rhinitis. (PubMed id 19222422)1, 4, 9 Benson M....Cardell L.O. (Allergy 2009)
    5. Association of atopy and eczema with polymorphisms in T-cell immunoglobulin domain and mucin domain-IL-2-inducible T-cell kinase gene cluster in chromosome 5 q 33. (PubMed id 16159638)1, 4, 9 Graves P.E....Martinez F.D. (J. Allergy Clin. Immunol. 2005)
    6. Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav. (PubMed id 12186560)1, 2, 9 Perez-Villar J.J.... Kanner S.B. (Biochemistry 2002)
    7. SH2-dependent autophosphorylation within the Tec family kinase Itk. (PubMed id 19523959)1, 2, 9 Joseph R.E.... Andreotti A.H. (J. Mol. Biol. 2009)
    8. Genome-wide association study of personality traits in bipolar patients. (PubMed id 21368711)1, 4 Alliey-Rodriguez N....Gershon E.S. (Psychiatr. Genet. 2011)
    9. Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells. (PubMed id 21725281)1, 2 Sela M....Yablonski D. (EMBO J. 2011)
    10. Development and validation of a method for profiling post-translational modification activities using protein microarrays. (PubMed id 20596523)1, 2 Del RincA^n S.V....Spruck C. (PLoS ONE 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 3702 HGNC: 6171 AceView: ITK Ensembl:ENSG00000113263 euGenes: HUgn3702
    ECgene: ITK Kegg: 3702 H-InvDB: ITK

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for ITK Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for ITK Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for ITK gene:
    Search GeneIP for patents involving ITK

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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