ISY1 Gene
protein-coding GIFtS: 48
GCID: GC03M128846
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|
ISY1 splicing factor homolog (S. cerevisiae)
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Aliases
for ISY1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| ISY1 Splicing Factor Homolog (S. Cerevisiae)1 2 | | KIAA11601 3 5 | | FSAP332 | | FSAP332 | | Functional Spliceosome-Associated Protein 332 | | Pre-MRNA-Splicing Factor ISY1 Homolog2 |
Export aliases for ISY1 gene to outside databasesPrevious GC identifers: GC03M130292 GC03M126234 |
Summaries
for ISY1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: ISY1_HUMAN, Q9ULR0Function: May play a role in pre-mRNA splicing (Potential)
|
Genomic Views
for ISY1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000003.11 NC_018914.1 NT_005612.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the ISY1 gene promoter:
E2F-3a E2F-4 Elk-1 E2F-5 AML1a STAT5A POU6F1 (c2) E2F-2 E2F-1 E2F
Other transcription factors
Search SABiosciences Chromatin IP Primers for ISY1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ISY1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 3q21.3 Ensembl cytogenetic band: 3q21.3 HGNC cytogenetic band: 3q21.3ISY1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03M128846: view genomic region
(about GC identifiers)
Start:
|
128,846,258 bp from pter |
End:
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128,880,136 bp from pter |
Size:
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33,879 bases |
Orientation:
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minus strand |
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Proteins
for ISY1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: ISY1_HUMAN, Q9ULR0 (See
protein sequence)Recommended Name: Pre-mRNA-splicing factor ISY1 homolog Size: 285 amino acids; 32992 Da
Subunit: Identified in the spliceosome C complex
Subcellular location: Nucleus (Potential)
Sequence caution: Sequence=BAA86474.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: Q96IL2 Q9BT05Alternative splicing: 3 isoforms: Q9ULR0-3 Q9ULR0-1 Q9ULR0-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for ISY1: NX_Q9ULR0
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9ULR0
ISY1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001186398.1 NP_065752.1
ENSEMBL proteins: ENSP00000376973 ENSP00000273541 ENSP00000426416 ENSP00000376970 ENSP00000422403
Human Recombinant Protein Products for ISY1:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
ISY1 for ontologies About GeneDecksing
ISY1 Antibody Products:
Assay Products for ISY1: |
Protein
Domains /
Families
for ISY1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
ISY1 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q9ULR0ProtoNet protein and cluster: Q9ULR0 UniProtKB/Swiss-Prot: ISY1_HUMAN, Q9ULR0Similarity: Belongs to the ISY1 family |
Function
for ISY1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
inGenious Targeting Laboratory,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase,
shRNA from
OriGene,
Sirion Biotech,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Sirion Biotech,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
Sirion Biotech,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Molecular Function:
UniProtKB/Swiss-Prot Summary: ISY1_HUMAN, Q9ULR0Function: May play a role in pre-mRNA splicing (Potential)Phenotypes:
2 GenomeRNAi human phenotypes for ISY1:
Animal Models:
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ISY1
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ISY1
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): ISY1 (NM_020701) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for ISY1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ISY1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISY1 |
|
Pathways & Interactions
for ISY1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | mRNA Splicing - Major Pathway | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 
Kegg Pathway (Kegg details for ISY1):
ISY1 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ISY1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 18)
5/19 Interacting proteins for ISY1 (Q9ULR02, 3 ENSP000003769734) via UniProtKB, MINT, STRING, and/or I2D (see all 19)About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0000398 | mRNA splicing, via spliceosome |
IC | 11991638 |
ISY1 for ontologies About GeneDecksing
|
Drugs & Compounds
for ISY1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for ISY1
Search CenterWatch for drugs/clinical trials and news about ISY1
|
Transcripts
for ISY1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
Sirion Biotech,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for ISY1 gene (2 alternative transcripts): NM_001199469.1 NM_020701.3 Unigene Cluster for ISY1: ISY1 splicing factor homolog (S. cerevisiae) Hs.512661 [show with all ESTs]Unigene Representative Sequence: NM_0012048907 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000393295(uc010hsz.2 uc010hta.2 uc003elp.2) ENST00000471497
ENST00000273541 ENST00000471306 ENST00000496163 ENST00000393292 ENST00000485703
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ISY1
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ISY1
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): ISY1 (NM_020701) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for ISY1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ISY1 |
Additional cDNA sequence: AK024035.1 BC004122.2 BC004442.1 BC007409.2 BC019849.1 HQ013244.1 14 DOTS entries: DT.456025 DT.100038093 DT.120862682 DT.92418662 DT.95229920 DT.100798218 DT.100690515 DT.100798230 DT.100679481 DT.100689789 DT.100038095 DT.95350688 DT.97851543 DT.120690482 24/393 AceView cDNA sequences (see all 393): BQ227078 BE909025 AI940191 BQ053774 BU855939 BU154744 BQ069284 BQ068663 BM558956 BU859626 BX418603 AL603611 BQ054564 CR596493 BQ051942 AW075243 BF219669 BM825702 AY166852 BQ052432 AI937413 AI202059 BX101105 NM_020701
GeneLoc Exon Structure
|
Expression
for ISY1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| ISY1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TTGACCGGAG
About this image
See ISY1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ISY1
SOURCE GeneReport for Unigene cluster: Hs.512661
SABiosciences Custom PCR Arrays for ISY1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ISY1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat ISY1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ISY1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ISY1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISY1 |
Orthologs
for ISY1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for ISY1 gene from 8/25 species (see all 25) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
ISY16 |
Uncharacterized protein |
89(a) |
1 → many |
12(5264146-5273910) |
lizard
(Anolis carolinensis) |
Reptilia |
ISY16 |
-- |
89(a) |
1 → many |
2(159139536-159160530) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.194642 |
Xenopus laevis transcribed sequence with weak similarity more |
76.38(n) |
|
BJ635570.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
rab436 |
RAB43, member RAS oncogene family |
81(a) |
1 → many |
11(461201-473071) |
fruit fly
(Drosophila melanogaster) |
Insecta |
CG96676 |
-- |
60(a) |
1 → many |
3R(3951009-3952055) |
worm
(Caenorhabditis elegans) |
Secernentea |
F53B7.36 |
Protein F53B7.3 |
46(a) |
1 → many |
V(10999406-11000773) |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
AT3G187906 |
pre-mRNA-splicing factor ISY1 |
46(a) |
1 → many |
3(6477699-6479548) |
rice
(Oryza sativa) |
Liliopsida |
-- |
pre-mRNA-splicing factor ISY1, putative, expressed... |
43(a) |
1 → many |
2(23572529-23574978) |
Species with no ortholog for ISY1
ENSEMBL Gene Tree for ISY1 (if available)
TreeFam Gene Tree for ISY1 (if available) |
Paralogs
for ISY1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for ISY1 gene
- ISY1-RAB432
1 SIMAP similar gene for ISY1 using alignment to 4 protein entries: ISY1_HUMAN (see all proteins):ISY1-RAB43
ISY1 for paralogs About GeneDecksing
|
Genomic Variants
for ISY1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 3 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for ISY1 (128846258 - 128880136 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for ISY1: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ISY1 |
|
Disorders
/ Diseases
for ISY1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
ISY1 for disorders About GeneDecksing
OMIM gene information: 612764
OMIM disorders: --
1  disease for ISY1: About MalaCardsmalaria
Export disorders for ISY1 gene to outside databases
|
Publications
for ISY1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for ISY1 gene, integrated from 9 sources (see all 14):
(articles sorted by number of sources associating them with ISY1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing. (PubMed id 16103217)1, 3 Villa T. and Guthrie C. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. (PubMed id 11991638)1, 2 Jurica M.S.... Moore M.J. (2002)
- Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. (PubMed id 10574461)1, 2 Hirosawa M.... Ohara O. (1999)
- A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
- System-wide changes to SUMO modifications in response to heat shock. (PubMed id 19471022)1 Golebiowski F....Hay R.T. (2009)
- Lysine acetylation targets protein complexes and co-regulates major cellular functions. (PubMed id 19608861)2 Choudhary C.... Mann M. (2009)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
|
External Searches for ISY1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing ISY1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing ISY1 gene
(According to HUGE)
About This Section
| |
Specialized Databases showing ISY1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for ISY1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for ISY1 gene:
Search GeneIP for patents involving ISY1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for ISY1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for ISY1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ISY1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ISY1 | | | OriGene Protein Over-expression Lysate for ISY1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for ISY1 | | OriGene 3'-UTR Clone for ISY1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ISY1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ISY1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for ISY1 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat ISY1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ISY1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ISY1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ISY1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ISY1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ISY1 |
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| |  |  |  |  | | | | |
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 | | | ISY1 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISY1 |
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