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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

ISG15 Gene

protein-coding GIFtS: 64
GCID: GC01P000938

ISG15 ubiquitin-like modifier

(Previous names: interferon, alpha-inducible protein (clone IFI-15K) )
(Previous symbol: G1P2)

Explore 40 diseases affiliated with
ISG15 via

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
ISG15 Ubiquitin-Like Modifier¹ ²		HUCRP¹
G1P2¹ ² ³ ⁵		Interferon-Induced 17-KDa/15-KDa Protein²
UCRP¹ ² ³		Interferon-Stimulated Protein, 15 KDa²
IFI15¹ ² ⁵		Ubiquitin-Like Protein ISG15²
Interferon, Alpha-Inducible Protein (Clone IFI-15K)¹ ²		Interferon-Induced 15 KDa Protein³
Ubiquitin Cross-Reactive Protein² ³		Interferon-Induced 17 KDa Protein³
IP17² ³

External Ids:	HGNC: 4053¹	Entrez Gene: 9636²	Ensembl: ENSG00000187608⁷	OMIM: 147571⁵	UniProtKB: P05161³

Export aliases for ISG15 gene to outside databases

Previous GC identifers: GC01P000478 GC01P000220

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ISG15:

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon

activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein,

including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments,

cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been

found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. (provided by RefSeq,

Dec 2012)

UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161

Function: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta

stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity

and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge

with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1,

MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity

towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a

trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also

be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by

inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections

Function: In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release

of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By

similarity)

Gene Wiki entry for ISG15

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000001.10 NC_018912.1 NT_004350.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the ISG15 gene promoter:
CREB p53 deltaCREB MyoD STAT3
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: ISG15 promoter sequence

Search SABiosciences Chromatin IP Primers for ISG15

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ISG15

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p36.33 Ensembl cytogenetic band: 1p36.33 HGNC cytogenetic band: 1p36.33

ISG15 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ISG15 gene location

GeneLoc information about chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01P000938: view genomic region (about GC identifiers)

Start:	948,803 bp from pter	End:	949,920 bp from pter
Size:	1,118 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161 (See protein sequence)

Recommended Name: Ubiquitin-like protein ISG15 precursor

Size: 165 amino acids; 17888 Da

Subunit: Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable).

Interaction with influenza B NS1 protein inhibits this conjugation

Subcellular location: Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the

intermediate filaments and distributed in a punctate pattern. Also secreted

6/7 PDB 3D structures from and Proteopedia for ISG15 (see all 7):

1Z2M (3D)

2HJ8 (3D)

3PHX (3D)

3PSE (3D)

3R66 (3D)

3RT3 (3D)

Secondary accessions: Q5SVA4 Q7Z2G2 Q96GF0

Explore the universe of human proteins at neXtProt for ISG15: NX_P05161

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P05161

ISG15 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_005092.1

ENSEMBL proteins:

ENSP00000368699

Reactome Protein details: P05161
Human Recombinant Protein Products:

	EMD Millipore Purified and/or Recombinant ISG15 Protein
	R&D Systems Recombinant & Natural Proteins for ISG15 (ISG15/UCRP)
	Enzo Life Sciences proteins for ISG15
	OriGene Purified Protein: ISG15 OriGene Protein Over-expression Lysate: ISG15 OriGene Custom Protein Services for ISG15
	GenScript Custom Purified and Recombinant Proteins Services for ISG15
	Novus Biologicals ISG15 Proteins Novus Biologicals ISG15 Lysates
	Browse Sino Biological Recombinant Proteins
	ProSpec Recombinant Protein for ISG15
	Uscn Proteins for ISG15

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005615	extracellular space	NAS	--
GO:0005737	cytoplasm	NAS	--
GO:0005829	cytosol	TAS	--

ISG15 for ontologies About GeneDecksing

ISG15 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of ISG15
	R&D Systems Antibodies for ISG15 (ISG15/UCRP)
	Cell Signaling Technology (CST) Antibodies for ISG15
	OriGene Antibodies (see all 3): ISG15 OriGene Custom Antibody Services for ISG15
	GenScript Custom Superior Antibodies Services for ISG15
	Novus Biologicals ISG15 Antibodies
	Abcam antibodies for ISG15
	Uscn Antibodies for ISG15
	ThermoFisher Antibodies for ISG15

Assay Products for ISG15:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems Ubiquitin Proteasome Pathway (UPP) Assay Kits & Reagents for ISG15 (ISG15/UCRP)
	GenScript Custom Assay Services for ISG15
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for ISG15

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ISG15 for domains About GeneDecksing

3 InterPro domains/families:

IPR019956 Ubiquitin_subgr

IPR000626 Ubiquitin

IPR019955 Ubiquitin_supergroup

Graphical View of Domain Structure for InterPro Entry P05161

ProtoNet protein and cluster: P05161

1 Blocks protein family: IPB000626 Ubiquitin domain

UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161

Similarity: Contains 2 ubiquitin-like domains

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: