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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ISG15 Gene

protein-coding   GIFtS: 66
GCID: GC01P000938

ISG15 Ubiquitin-Like Modifier

(Previous names: interferon, alpha-inducible protein (clone IFI-15K))
(Previous symbol: G1P2)
Microbiology & Infectious Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
ISG15 Ubiquitin-Like Modifier1 2     IFI152 5
G1P21 2 3 5     Interferon-Induced 17-KDa/15-KDa Protein2
Interferon, Alpha-Inducible Protein (Clone IFI-15K)1 2     Interferon-Stimulated Protein, 15 KDa2
Ubiquitin Cross-Reactive Protein2 3     Ubiquitin-Like Protein ISG152
IP172 3     Interferon-Induced 15 KDa Protein3
UCRP2 3     Interferon-Induced 17 KDa Protein3
hUCRP2 3     

External Ids:    HGNC: 40531   Entrez Gene: 96362   Ensembl: ENSG000001876087   OMIM: 1475715   UniProtKB: P051613   

Export aliases for ISG15 gene to outside databases

Previous GC identifers: GC01P000478 GC01P000220


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ISG15 Gene:
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins
upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded
protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate
filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this
protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes
secreted. (provided by RefSeq, Dec 2012)

GeneCards Summary for ISG15 Gene: 
ISG15 (ISG15 ubiquitin-like modifier) is a protein-coding gene. Diseases associated with ISG15 include ebola hemorrhagic fever, and mendelian susceptibility to mycobacterial diseases due to complete isg15 deficiency, and among its related super-pathways are RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways and Immune response IFN alpha/beta signaling pathway. GO annotations related to this gene include protein tag. An important paralog of this gene is UBD.

UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161
Function: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta
stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate
specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but
seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1,
SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows
specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic
factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to
intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell
signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display
antiviral activity during viral infections
Function: In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the
release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy
(By similarity)

Gene Wiki entry for ISG15 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.2  NT_004350.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ISG15 gene promoter:
         CREB   p53   deltaCREB   MyoD   STAT3   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidISG15 promoter sequence
   Search SABiosciences Chromatin IP Primers for ISG15

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ISG15


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p36.33   Ensembl cytogenetic band:  1p36.33   HGNC cytogenetic band: 1p36.33

ISG15 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ISG15 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P000938:  view genomic region     (about GC identifiers)

Start:
948,803 bp from pter      End:
949,920 bp from pter
Size:
1,118 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161 (See protein sequence)
Recommended Name: Ubiquitin-like protein ISG15 precursor  
Size: 165 amino acids; 17888 Da
Subunit: Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme)
(Probable). Interaction with influenza B NS1 protein inhibits this conjugation
Subcellular location: Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the
intermediate filaments and distributed in a punctate pattern. Also secreted
6/7 PDB 3D structures from and Proteopedia for ISG15 (see all 7):
1Z2M (3D)        2HJ8 (3D)        3PHX (3D)        3PSE (3D)        3R66 (3D)        3RT3 (3D)    
Secondary accessions: Q5SVA4 Q7Z2G2 Q96GF0

Explore the universe of human proteins at neXtProt for ISG15: NX_P05161

Explore proteomics data for ISG15 at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_P05161

  • ISG15 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    ISG15 Protein Expression
    REFSEQ proteins: NP_005092.1  
    ENSEMBL proteins: 
     ENSP00000368699  
    Reactome Protein details: P05161
    Human Recombinant Protein Products for ISG15: 
    EMD Millipore Purified and/or Recombinant ISG15 Protein
    R&D Systems Recombinant & Natural Proteins for ISG15 (ISG15/UCRP)
    Enzo Life Sciences proteins for ISG15
    OriGene Purified Protein for ISG15
    OriGene Protein Over-expression Lysate for ISG15
    OriGene MassSpec for ISG15 
    OriGene Custom Protein Services for ISG15
    GenScript Custom Purified and Recombinant Proteins Services for ISG15
    Novus Biologicals ISG15 Proteins
    Novus Biologicals ISG15 Lysates
    Sino Biological Recombinant Protein for ISG15
    Browse Sino Biological Cell Lysates 
    ProSpec Recombinant Protein for ISG15
    Browse Proteins at Cloud-Clone Corp. 

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region IDA--
    GO:0005615extracellular space NAS--
    GO:0005737cytoplasm NAS--
    GO:0005829cytosol TAS--

    ISG15 for ontologies           About GeneDecksing



    ISG15 Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of ISG15
    R&D Systems Antibodies for ISG15 (ISG15/UCRP)
    Cell Signaling Technology (CST) Antibodies for ISG15 
    OriGene Antibodies for ISG15
    OriGene Custom Antibody Services for ISG15
    GenScript Custom Superior Antibodies Services for ISG15
    Novus Biologicals ISG15 Antibodies
    Abcam antibodies for ISG15
    Browse Antibodies at Cloud-Clone Corp. 
    ThermoFisher Antibodies for ISG15
    LSBio Antibodies in human, mouse, rat for ISG15 

    Assay Products for ISG15: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ISG15
    R&D Systems Ubiquitin Proteasome Pathway (UPP) Assay Kits & Reagents for ISG15 (ISG15/UCRP)
    GenScript Custom Assay Services for ISG15
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp. 
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    3 InterPro protein domains:
     IPR000626 Ubiquitin
     IPR019955 Ubiquitin_supergroup
     IPR019956 Ubiquitin_subgr

    Graphical View of Domain Structure for InterPro Entry P05161

    ProtoNet protein and cluster: P05161

    1 Blocks protein domain: IPB000626 Ubiquitin domain

    UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161
    Similarity: Contains 2 ubiquitin-like domains


    ISG15 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ISG15_HUMAN, P05161
    Function: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta
    stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate
    specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but
    seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1,
    SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows
    specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic
    factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to
    intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell
    signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display
    antiviral activity during viral infections
    Function: In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the
    release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy
    (By similarity)
    Induction: By type I interferons

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI16872604
    GO:0031386protein tag IEA--
         
    ISG15 for ontologies           About GeneDecksing


    Phenotypes:
         4 GenomeRNAi human phenotypes for ISG15:
     Decreased influenza A H1N1 (A/  Decreased influenza A H1N1 (A/  Decreased influenza A/WSN/33 r  Increased cell death HMECs cel 

         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Isg15):
     immune system  skeleton 

    ISG15 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Isg15tm1Kpk for ISG15

       inGenious Targeting Laboratory - Custom generated mouse model solutions for ISG15 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for ISG15

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ISG15 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ISG15 

    miRNA
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    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for ISG15
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ISG15

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ISG15
    Sirion Biotech Customized adenovirus for overexpression of ISG15

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for ISG15 (see all 8)
    OriGene ORF clones in mouse, rat for ISG15
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ISG15 (NM_005101)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ISG15
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ISG15
    Sirion Biotech Customized lentivirus for stable overexpression of ISG15 
                         Customized lentivirus expression plasmids for stable overexpression of ISG15 

    Cell Line
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    Search LifeMap BioReagents cell lines for ISG15
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISG15


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for ISG15 About   (see all 6)                                                                                              See pathways by source

    SuperPathContained pathways About
    1RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
    RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways0.52
    Negative regulators of RIG-I/MDA5 signaling0.49
    RIG-I-like receptor signaling pathway0.52
    2Immune response IFN alpha/beta signaling pathway
    Immune response IFN alpha/beta signaling pathway0.71
    Interferon alpha/beta signaling0.45
    3Interferon Signaling
    Interferon Signaling0.61
    Antiviral mechanism by IFN-stimulated genes0.42
    Cytokine Signaling in Immune system0.61
    ISG15 antiviral mechanism0.42
    4Immune System
    Immune System0.56
    Innate Immune System0.50
    5Type II interferon signaling (IFNG)
    Type II interferon signaling (IFNG)

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 EMD Millipore Pathway for ISG15
        Immune response IFN alpha/beta signaling pathway


    1 Cell Signaling Technology (CST) Pathway for ISG15
        Protein Stability

    1 GeneGo (Thomson Reuters) Pathway for ISG15
        Immune response IFN alpha/beta signaling pathway

    1 BioSystems Pathway for ISG15
        Type II interferon signaling (IFNG)


    5/9        Reactome Pathways for ISG15 (see all 9)
        Antiviral mechanism by IFN-stimulated genes
    Interferon alpha/beta signaling
    Cytokine Signaling in Immune system
    RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
    Interferon Signaling


    1         Kegg Pathway  (Kegg details for ISG15):
        RIG-I-like receptor signaling pathway


    ISG15 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for ISG15

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/78 Interacting proteins for ISG15 (P051612, 3 ENSP000003686994) via UniProtKB, MINT, STRING, and/or I2D (see all 78)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    FLNBO753692, 3, ENSP000002959564MINT-7288599 MINT-7288590 MINT-7288640 MINT-7288670 I2D: score=1 STRING: ENSP00000295956
    PPM1BO756882, 3, ENSP000002824124MINT-2841892 MINT-2841870 I2D: score=2 STRING: ENSP00000282412
    UBA7P412262, 3, ENSP000003332664MINT-68321 I2D: score=6 STRING: ENSP00000333266
    USP18Q9UMW82, 3, ENSP000002157944MINT-66847 I2D: score=5 STRING: ENSP00000215794
    DDX58O957863, ENSP000003692134I2D: score=1 STRING: ENSP00000369213
    About this table

    Gene Ontology (GO): 5/14 biological process terms (GO ID links to tree view) (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007267cell-cell signaling NAS--
    GO:0019048modulation by virus of host morphology or physiology IEA--
    GO:0019221cytokine-mediated signaling pathway TAS--
    GO:0019941modification-dependent protein catabolic process IEA--
    GO:0031397negative regulation of protein ubiquitination IDA18305167

    ISG15 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    ISG15 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ISG15

    10/12 Novoseek inferred chemical compound relationships for ISG15 gene (see all 12)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    2',5'-oligoadenylate 41.9 2 17156188 (1), 15749911 (1)
    amide 30.6 4 18478335 (1), 20053572 (1)
    cycloheximide 22.9 2 1919073 (1)
    cysteine 11.9 10 18606809 (2), 10030672 (1), 16407192 (1)
    camptothecin 9.2 8 15684817 (5), 11193908 (1)
    lysine 8.87 1 20133869 (1)
    hydrogen 5.49 6 10993253 (1), 11955021 (1), 15213422 (1), 15213429 (1)
    atp 0 5 8550581 (2), 12426315 (1)
    progesterone 0 5 10684803 (3), 17088421 (2)
    methanol 0 1 17525462 (1)

    Search CenterWatch for drugs/clinical trials and news about ISG15

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ISG15 gene: 
    NM_005101.3  

    Unigene Cluster for ISG15:

    ISG15 ubiquitin-like modifier
    Hs.458485  [show with all ESTs]
    Unigene Representative Sequence: BQ279256
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000379389(uc001acj.4)
    Congresses - knowledge worth sharing:  
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    Search QIAGEN for miScript miRNA Assays for microRNAs that regulate ISG15
    Browse SwitchGear 3'UTR luciferase reporter plasmids
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    Clone
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    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ISG15
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ISG15
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ISG15
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ISG15

    Additional mRNA sequence: 

    AY168648.1 BC009507.2 BT007297.1 M13755.1 

    4 DOTS entries:

    DT.417017  DT.100787717  DT.100060240  DT.121453518 

    24/231 AceView cDNA sequences (see all 231):

    AI537436 AI086993 CK904944 BM055105 BU161226 BG337208 AI423923 BM855102 
    BF036894 CB140944 BI836784 AW439384 AI470150 BE889848 BU521778 BQ437232 
    BF684203 BF971689 CB136953 BM712238 BU184485 BG576634 BU521125 BU194879 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ISG15 expression in normal human tissues (normalized intensities)      ISG15 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AATCTGCGCC
    ISG15 Expression
    About this image


    ISG15 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/2 selected tissues (see all 2) fully expand
     
     Ovary (Reproductive System)
             Secondary follicles
     
     Bone (Muscoskeletal System)
             Human fetal bone marrow-derived mesenchymal stromal cells

    See ISG15 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ISG15

    SOURCE GeneReport for Unigene cluster: Hs.458485

    UniProtKB/Swiss-Prot: ISG15_HUMAN, P05161
    Tissue specificity: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons

        SABiosciences Expression via Pathway-Focused PCR Arrays including ISG15: 
              Antiviral Response in human mouse rat
              Interferons & Receptors in human mouse rat
              Type I Interferon Response in human mouse rat
              JAK / STAT Signaling Pathway in human mouse rat

    Primer
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    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ISG15
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISG15

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals and fungi.

    Orthologs for ISG15 gene from 5/10 species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Isg151 , 5 ISG15 ubiquitin-like modifier1, 5 76.5(n)1
    66.67(a)1
      4 (88.61 cM)5
    1000388821  NM_015783.31  NP_056598.21 
     1561994245 
    lizard
    (Anolis carolinensis)
    Reptilia ISG156
    ISG15 ubiquitin-like modifier
    37(a)
    1 ↔ 1
    GL343417.1(420256-422394)
    zebrafish
    (Danio rerio)
    Actinopterygii isg156
    ISG15 ubiquitin-like modifier
    34(a)
    1 ↔ 1
    5(21895629-21896102)
    worm
    (Caenorhabditis elegans)
    Secernentea F52C6.46
    F52C6.36
    (see all 3)
    Protein F52C6.3
    (see all 3)
    17(a)
    13(a)
    (see all 3)
    many ↔ many
    many ↔ many
    (see all 3)
    II(1923147-1923705)
    II(1924757-1925716)
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes UBI46
    Ubiquitin, becomes conjugated to proteins, marking...
    13(a)
    1 → many
    XII(64062-65207)


    ENSEMBL Gene Tree for ISG15 (if available)
    TreeFam Gene Tree for ISG15 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ISG15 gene
    UBD2  
    6 SIMAP similar genes for ISG15 using alignment to 1 protein entry:     ISG15_HUMAN:
    UBA52    DKFZp434K0435    UBB    UbC    UBC    UBBP4

    ISG15 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    4 SNPs in ISG15 are shown    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0161814
    ----see VAR_0161812 S N mis40--------
    rs593085461,2
    C--948423(+) AACAA-/ACA   
       A
    /CAA
    AAAAA
    2 -- us2k1 cds11NA 2
    rs709495411,2
    C--950117(+) TGAAG-/TAAG  
            
    ACAGC
    1 -- ds50011Minor allele frequency- TAAG:0.00NA 2
    rs725027441,2
    ----948431(+) CAACA-/CAAAAAAA 1 -- cds10--------

    HapMap Linkage Disequilibrium report for ISG15 (948803 - 949920 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 10/18 variations for ISG15 (see all 18):    About this table     
    Variant IDTypeSubtypePubMed ID
    nsv509146CNV Insertion20534489
    dgv14n71CNV Loss21882294
    dgv9n71CNV Loss21882294
    dgv12n71CNV Loss21882294
    dgv10n71CNV Loss21882294
    dgv13n71CNV Loss21882294
    dgv11n71CNV Loss21882294
    nsv871483CNV Loss21882294
    nsv871547CNV Loss21882294
    dgv15n71CNV Loss21882294


    Human Gene Mutation Database (HGMD): ISG15
    SABiosciences Cancer Mutation PCR Assays
    SeqTarget long-range PCR primers for resequencing ISG15
    DNA2.0 Custom Variant and Variant Library Synthesis for ISG15

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 147571    OMIM disorders: --

    20/42 diseases for ISG15 (see all 42):    About MalaCards
    ebola hemorrhagic fever    mendelian susceptibility to mycobacterial diseases due to complete isg15 deficiency    frontotemporal dementia    mucolipidosis
    acute promyelocytic leukemia    influenza    lupus nephritis    dermatomyositis
    dementia    hemorrhagic fever    nephritis    lateral sclerosis
    hepatitis c    ataxia telangiectasia    amyotrophic lateral sclerosis    parkinson's disease
    oral cancer    lupus erythematosus    liver disease    systemic lupus erythematosus

    1 disease from the University of Copenhagen DISEASES database for ISG15:
    Hepatitis C

    ISG15 for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:  
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    10/23 Novoseek inferred disease relationships for ISG15 gene (see all 23)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    vesicular stomatitis 37.3 2 17227866 (1)
    virus infection 34.9 8 17227866 (3), 1370126 (1), 16697044 (1), 10508226 (1)
    influenza 29.5 20 19357168 (7), 20133869 (4), 16009940 (2), 1370126 (1) (see all 6)
    neurodegeneration 29.2 1 18550655 (1)
    liver diseases alcoholic 24.9 2 12436058 (1), 12792671 (1)
    frontotemporal dementia 24.7 2 19223871 (1), 17021754 (1)
    neurodegenerative diseases 24.4 5 2172853 (1), 9228278 (1), 8861020 (1), 12792671 (1)
    amyotrophic lateral sclerosis 22.3 3 19223871 (1), 8861020 (1)
    parkinson disease 15.6 1 11841562 (1)
    bacterial infections 11.2 3 16352599 (1), 16407192 (1)

    Genetic Association Database (GAD): ISG15
    Human Genome Epidemiology (HuGE) Navigator: ISG15 (7 documents)

    Export disorders for ISG15 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ISG15 gene, integrated from 9 sources (see all 237):
    (articles sorted by number of sources associating them with ISG15)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular characterization of the interferon-induced 15-kDa protein. Molecular cloning and nucleotide and amino acid sequence. (PubMed id 3087979)1, 2, 3 Blomstrom D.C.... Knight E. Jr. (1986)
    2. Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern. (PubMed id 7526157)1, 2, 9 Loeb K.R. and Haas A.L. (1994)
    3. Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways. (PubMed id 16009940)1, 2, 9 Zhao C.... Krug R.M. (2005)
    4. The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein. (PubMed id 15131269)1, 2, 9 Zhao C.... Krug R.M. (2004)
    5. Conjugation of the 15-kDa interferon-induced ubiquitin homolog is distinct from that of ubiquitin. (PubMed id 8550581)1, 2, 9 Narasimhan J.... Haas A.L. (1996)
    6. Immunohistochemical localization of ubiquitin cross-reactive protein in human tissues. (PubMed id 7490683)1, 2, 9 Lowe J.... Mayer R.J. (1995)
    7. Crystal structure of the interferon-induced ubiquitin-like protein ISG15. (PubMed id 15917233)1, 2, 9 Narasimhan J....Kim J.J. (2005)
    8. Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility 'hot-spot'. (PubMed id 20628624)1, 4 Johnatty S.E.... . (2010)
    9. Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (2010)
    10. High resolution linkage and linkage disequilibrium analyses of chromosome 1p36 SNPs identify new positional candidate genes for low bone mineral density. (PubMed id 18597038)1, 4 Zhang H....Devoto M. (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 9636 HGNC: 4053 AceView: G1P2 Ensembl:ENSG00000187608 euGenes: HUgn9636
    ECgene: ISG15 Kegg: 9636 H-InvDB: ISG15

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ISG15 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ISG15 gene:
    Search GeneIP for patents involving ISG15

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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    About This Section

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