ISCA2 Gene
protein-coding GIFtS: 47
GCID: GC14P074960
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iron-sulfur cluster assembly 2 homolog (S. cerevisiae)(Previous name: HesB like domain containing 1 )
(Previous symbol: HBLD1)
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Aliases
for ISCA2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Iron-Sulfur Cluster Assembly 2 Homolog (S. Cerevisiae)1 2 | | HesB Like Domain Containing 11 | | HBLD11 2 3 | | C14_55571 | | ISA21 2 | | Iron-Sulfur Cluster Assembly 2 Homolog, Mitochondrial2 | | HESB-Like Domain-Containing Protein 12 3 | | |
Export aliases for ISCA2 gene to outside databasesPrevious GC identifers: GC14P074031 GC14P055128 |
Summaries
for ISCA2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for ISCA2:
The protein encoded by this gene is an A-type iron-sulfur cluster (ISC) protein found in mitochondria. The encodedprotein appears to be involved in the maturation of mitochondrial iron-sulfur proteins. Two transcript variantsencoding different isoforms have been found for this gene. (provided by RefSeq, Dec 2012)
UniProtKB/Swiss-Prot: ISCA2_HUMAN, Q86U28Function: Involved in the assembly of mitochondrial iron-sulfur proteins. Probably involved in the binding of anintermediate of Fe/S cluster assembly (By similarity)
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Genomic Views
for ISCA2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000014.8 NC_018925.1 NT_026437.12
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the ISCA2 gene promoter:
GR Elk-1 AP-1 NRSF form 1 HTF NRSF form 2 POU2F1 FOXO4 POU2F1a ATF6
Other transcription factors
Search SABiosciences Chromatin IP Primers for ISCA2
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ISCA2 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 14q24.3 Ensembl cytogenetic band: 14q24.3 HGNC cytogenetic band: 14q24.2ISCA2 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 14 GeneLoc Exon Structure GeneLoc location for GC14P074960: view genomic region
(about GC identifiers)
Start:
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74,960,423 bp from pter |
End:
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74,963,809 bp from pter |
Size:
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3,387 bases |
Orientation:
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plus strand |
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Proteins
for ISCA2 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: ISCA2_HUMAN, Q86U28 (See
protein sequence)Recommended Name: Iron-sulfur cluster assembly 2 homolog, mitochondrial precursor Size: 154 amino acids; 16476 Da
Subcellular location: Mitochondrion (By similarity)
Sequence caution: Sequence=CAD62580.1; Type=Erroneous initiation;
Secondary accessions: A6NFF1 A8K3W3 Q8IYZ0 Q96BB2Explore the universe of human proteins at neXtProt for ISCA2: NX_Q86U28
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q86U28
ISCA2 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001258936.1 NP_919255.2
ENSEMBL proteins: ENSP00000452007 ENSP00000298818 ENSP00000450523
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005739 | mitochondrion |
IEA | -- |
ISCA2 for ontologies About GeneDecksing
ISCA2 Antibody Products:
Assay Products for ISCA2: |
Protein
Domains /
Families
for ISCA2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
ISCA2 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q86U28ProtoNet protein and cluster: Q86U28 1 Blocks protein family: IPB000361 Protein of unknown function
UniProtKB/Swiss-Prot: ISCA2_HUMAN, Q86U28Similarity: Belongs to the HesB/IscA family |
Function
for ISCA2 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: ISCA2_HUMAN, Q86U28Function: Involved in the assembly of mitochondrial iron-sulfur proteins. Probably involved in the binding of anintermediate of Fe/S cluster assembly (By similarity)
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ISCA2
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISCA2 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005198 | structural molecule activity |
IEA | -- | | GO:0046872 | metal ion binding |
IEA | -- | | GO:0051536 | iron-sulfur cluster binding |
IEA | -- |
ISCA2 for ontologies About GeneDecksing
|
Pathways & Interactions
for ISCA2 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ISCA2
STRING Interaction
Network Preview (showing 4 interactants - click image to see more details)
5 Interacting proteins for ISCA2 (Q86U283 ENSP000002988184) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016226 | iron-sulfur cluster assembly |
IEA | -- |
ISCA2 for ontologies About GeneDecksing
|
Drugs & Compounds
for ISCA2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for ISCA2
1 HMDB Compound for ISCA2 About this table
| Compound | Synonyms |
CAS
# | PubMed Ids |
|---|
| Iron | Armco iron (see all 19) | 7439-89-6 | -- |
Search CenterWatch for drugs/clinical trials and news about ISCA2
|
Transcripts
for ISCA2 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for ISCA2 gene (2 alternative transcripts): NM_001272007.1 NM_194279.3 Unigene Cluster for ISCA2: Iron-sulfur cluster assembly 2 homolog (S. cerevisiae) Hs.291079 [show with all ESTs]Unigene Representative Sequence: BQ2248014 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000556816(uc001xpz.2) ENST00000298818 ENST00000554924 ENST00000555139
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ISCA2 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ISCA2
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: ISCA2 (NM_194279) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for ISCA2 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ISCA2 |
Additional cDNA sequence: AK290728.1 BC015771.1 BC032893.2 BX248252.1 7 DOTS entries: DT.100032518 DT.100794204 DT.313533 DT.97780846 DT.100835373 DT.120749213 DT.86842811 24/136 AceView cDNA sequences (see all 136): BU846230 AI146474 BX248252 BQ917404 AI337254 BM990811 BQ017457 BM919603 AW779083 BQ011674 AW960308 BM761557 CD675798 AI218065 AI301334 BU631516 CD671525 AI080185 AI797561 AI610282 AI218909 CB114594 AI199880 BU630812
GeneLoc Exon Structure
2 Alternative Splicing Database (ASD) splice patterns (SP) for ISCA2 About this scheme
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b |
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| SP1: | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | |
ECgene alternative splicing isoforms for ISCA2
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Expression
for ISCA2 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| ISCA2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GCTCTAAAAT
About this image
See ISCA2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ISCA2
SOURCE GeneReport for Unigene cluster: Hs.291079
SABiosciences Custom PCR Arrays for ISCA2
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ISCA2
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat ISCA2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ISCA2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ISCA2 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISCA2 |
Orthologs
for ISCA2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the last universal common ancestor (LUCA).
Orthologs for ISCA2 gene from 9/28 species (see all 28) About this table
ENSEMBL Gene Tree for ISCA2 (if available)
TreeFam Gene Tree for ISCA2 (if available) |
Paralogs
for ISCA2 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for ISCA2 gene
1 SIMAP similar gene for ISCA2 using alignment to 3 protein entries: ISCA2_HUMAN (see all proteins):ISCA1
ISCA2 for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for ISCA2
PGOHUM00000246300
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Genomic Variants
for ISCA2 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 14 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for ISCA2 (74960423 - 74963809 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for ISCA2: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ISCA2 |
|
Disorders
/ Diseases
for ISCA2 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
ISCA2 for disorders About GeneDecksing
4  diseases for ISCA2: About MalaCardshemophilia tuberculosis malaria mycobacterium tuberculosis
Human Genome Epidemiology (HuGE) Navigator: ISCA2 (4 documents)
Export disorders for ISCA2 gene to outside databases
|
Publications
for ISCA2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for ISCA2 gene integrated from 9 sources:
(articles sorted by number of sources associating them with ISCA2) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- The human mitochondrial ISCA1, ISCA2, and IBA57 protei ns are required for [4Fe-4S] protein maturation. (PubMed id 22323289)1 Sheftel A.D....Lill R. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- Genetic variants in nuclear-encoded mitochondrial gen es influence AIDS progression. (PubMed id 20877624)1 Hendrickson S.L....O'Brien S.J. (2010)
- Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1 Bailey S.D....Anand S. (2010)
- Gene-centric association signals for lipids and apoli poproteins identified via the HumanCVD BeadChip. (PubMed id 19913121)1 Talmud P.J.... . (2009)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
- The DNA sequence and analysis of human chromosome 14. (PubMed id 12508121)2 Heilig R.... Weissenbach J. (2003)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )2 Aoki H.... Hino O. (2000)
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External Searches for ISCA2 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing ISCA2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing ISCA2 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing ISCA2 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for ISCA2 | Pharmacogenomics, SNPs, Pathways |
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| | |
About This Section
| Patent Information for ISCA2 gene:
Search GeneIP for patents involving ISCA2
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for ISCA2 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for ISCA2 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ISCA2 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ISCA2 | | | OriGene Protein Over-expression Lysate for ISCA2 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for ISCA2 | | OriGene 3'-UTR Clone for ISCA2 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ISCA2 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ISCA2 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for ISCA2 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for ISCA2 | | OriGene Custom Protein Services for ISCA2 | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat ISCA2 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ISCA2 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ISCA2 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ISCA2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ISCA2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ISCA2 |
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 |  |  |  | |
| | | | Search Tocris compounds for ISCA2 |
| |  |  |  |  | | | | |
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 | | | ISCA2 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ISCA2 |
|  |  |  | | | | ThermoFisher Antibodies for ISCA2 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ISCA2 |
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