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Aliases for INSIG1 Gene

Aliases for INSIG1 Gene

  • Insulin Induced Gene 1 2 3
  • INSIG-1 Membrane Protein 2 3
  • Insulin-Induced Gene 1 Protein 3
  • INSIG-1 4
  • CL-6 3
  • CL6 3

External Ids for INSIG1 Gene

Previous GeneCards Identifiers for INSIG1 Gene

  • GC07U990053
  • GC07P154481
  • GC07P154527
  • GC07P154720
  • GC07P155089
  • GC07P148822

Summaries for INSIG1 Gene

Entrez Gene Summary for INSIG1 Gene

  • Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jun 2009]

GeneCards Summary for INSIG1 Gene

INSIG1 (Insulin Induced Gene 1) is a Protein Coding gene. Among its related pathways are Regulation of Cholesterol Biosynthesis by SREBP (SREBF) and Metabolism. An important paralog of this gene is INSIG2.

UniProtKB/Swiss-Prot for INSIG1 Gene

  • Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.

Gene Wiki entry for INSIG1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for INSIG1 Gene

Genomics for INSIG1 Gene

Regulatory Elements for INSIG1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for INSIG1 Gene

Start:
155,297,776 bp from pter
End:
155,310,235 bp from pter
Size:
12,460 bases
Orientation:
Plus strand

Genomic View for INSIG1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for INSIG1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for INSIG1 Gene

Proteins for INSIG1 Gene

  • Protein details for INSIG1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O15503-INSI1_HUMAN
    Recommended name:
    Insulin-induced gene 1 protein
    Protein Accession:
    O15503
    Secondary Accessions:
    • A4D2N1
    • A8K6L0
    • Q53XW8
    • Q9BUV5

    Protein attributes for INSIG1 Gene

    Size:
    277 amino acids
    Molecular mass:
    29987 Da
    Quaternary structure:
    • Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with HMGCR (via its SSD); the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with AMFR/gp78 (via its membrane domain); the interaction recruits HMCR at the ER membrane for its ubiquitination and degradation by the sterol-mediated ERAD pathway.
    Miscellaneous:
    • Expressed at high levels when nuclear SREBP levels are high as a result of sterol deprivation

    Three dimensional structures from OCA and Proteopedia for INSIG1 Gene

    Alternative splice isoforms for INSIG1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for INSIG1 Gene

Proteomics data for INSIG1 Gene at MOPED

Post-translational modifications for INSIG1 Gene

  • Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
  • Ubiquitination at Lys33, Lys156, Lys158, and Lys273
  • Modification sites at PhosphoSitePlus

Other Protein References for INSIG1 Gene

No data available for DME Specific Peptides for INSIG1 Gene

Domains for INSIG1 Gene

Protein Domains for INSIG1 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for INSIG1 Gene

Graphical View of Domain Structure for InterPro Entry

O15503

UniProtKB/Swiss-Prot:

INSI1_HUMAN :
  • O15503
Family:
  • Belongs to the INSIG family.
genes like me logo Genes that share domains with INSIG1: view

No data available for Gene Families for INSIG1 Gene

Function for INSIG1 Gene

Molecular function for INSIG1 Gene

GENATLAS Biochemistry: insulin induced gene 1,playing a role in growth and differention of tissues involved in metabolic control
UniProtKB/Swiss-Prot Function: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
UniProtKB/Swiss-Prot Induction: By insulin

Gene Ontology (GO) - Molecular Function for INSIG1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 12202038
genes like me logo Genes that share ontologies with INSIG1: view
genes like me logo Genes that share phenotypes with INSIG1: view

Animal Models for INSIG1 Gene

MGI Knock Outs for INSIG1:

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for INSIG1

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for INSIG1 Gene

Localization for INSIG1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for INSIG1 Gene

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for INSIG1 Gene COMPARTMENTS Subcellular localization image for INSIG1 gene
Compartment Confidence
endoplasmic reticulum 5
plasma membrane 3
golgi apparatus 1
mitochondrion 1
nucleus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for INSIG1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IDA 12202038
GO:0005789 endoplasmic reticulum membrane TAS --
GO:0016021 integral component of membrane --
GO:0032937 SREBP-SCAP-Insig complex IDA 15899885
genes like me logo Genes that share ontologies with INSIG1: view

Pathways for INSIG1 Gene

genes like me logo Genes that share pathways with INSIG1: view

Pathways by source for INSIG1 Gene

1 Qiagen pathway for INSIG1 Gene
1 BioSystems pathway for INSIG1 Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for INSIG1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006641 triglyceride metabolic process IEA --
GO:0006695 cholesterol biosynthetic process IEA --
GO:0006991 response to sterol depletion --
GO:0008152 metabolic process TAS 9268630
GO:0008203 cholesterol metabolic process --
genes like me logo Genes that share ontologies with INSIG1: view

Compounds for INSIG1 Gene

(3) Novoseek inferred chemical compound relationships for INSIG1 Gene

Compound -log(P) Hits PubMed IDs
sterol 76.7 5
cholesterol 49.2 9
lipid 20.1 3
genes like me logo Genes that share compounds with INSIG1: view

Transcripts for INSIG1 Gene

Unigene Clusters for INSIG1 Gene

Insulin induced gene 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for INSIG1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for INSIG1 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d
SP1: - - -
SP2: -
SP3: - - - -
SP4: - - - - - -
SP5: -
SP6: -

Relevant External Links for INSIG1 Gene

GeneLoc Exon Structure for
INSIG1
ECgene alternative splicing isoforms for
INSIG1

Expression for INSIG1 Gene

mRNA expression in normal human tissues for INSIG1 Gene

mRNA differential expression in normal tissues according to GTEx for INSIG1 Gene

This gene is overexpressed in Liver (10.2).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for INSIG1 Gene

SOURCE GeneReport for Unigene cluster for INSIG1 Gene Hs.520819

mRNA Expression by UniProt/SwissProt for INSIG1 Gene

O15503-INSI1_HUMAN
Tissue specificity: Expressed in all tissues tested with highest expression in the liver.
genes like me logo Genes that share expressions with INSIG1: view

In Situ Assay Products

Orthologs for INSIG1 Gene

This gene was present in the common ancestor of chordates.

Orthologs for INSIG1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia INSIG1 35
  • 99.64 (n)
  • 99.28 (a)
INSIG1 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia INSIG1 35
  • 81.52 (n)
  • 85.51 (a)
INSIG1 36
  • 86 (a)
OneToOne
dog
(Canis familiaris)
Mammalia INSIG1 35
  • 80.44 (n)
  • 85.24 (a)
INSIG1 36
  • 85 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Insig1 35
  • 81.34 (n)
  • 84.94 (a)
Insig1 16
Insig1 36
  • 82 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia INSIG1 36
  • 81 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia INSIG1 36
  • 83 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Insig1 35
  • 80.57 (n)
  • 85.33 (a)
chicken
(Gallus gallus)
Aves INSIG1 35
  • 78.49 (n)
  • 84.68 (a)
INSIG1 36
  • 98 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia INSIG1 36
  • 79 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia insig1 35
  • 71.07 (n)
  • 83.2 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2198 35
zebrafish
(Danio rerio)
Actinopterygii insig1 35
  • 67.86 (n)
  • 76.49 (a)
insig1 36
  • 76 (a)
OneToOne
wufi35b10 35
Species with no ortholog for INSIG1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for INSIG1 Gene

ENSEMBL:
Gene Tree for INSIG1 (if available)
TreeFam:
Gene Tree for INSIG1 (if available)

Paralogs for INSIG1 Gene

Paralogs for INSIG1 Gene

Selected SIMAP similar genes for INSIG1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with INSIG1: view

Variants for INSIG1 Gene

Sequence variations from dbSNP and Humsavar for INSIG1 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs2721 -- 155,296,033(+) ACACA(G/T)AAATC upstream-variant-2KB
rs14334 -- 155,296,568(+) GTGGG(C/T)AGCTT upstream-variant-2KB
rs1128636 -- 155,296,661(-) GCATA(A/G)GTGCT upstream-variant-2KB
rs1128640 -- 155,296,108(-) CAGAC(C/T)ACCCC upstream-variant-2KB
rs1129825 - 155,298,364(+) CCAGC(A/G)CCGCG missense, reference

Relevant External Links for INSIG1 Gene

HapMap Linkage Disequilibrium report
INSIG1
Human Gene Mutation Database (HGMD)
INSIG1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for INSIG1 Gene

Disorders for INSIG1 Gene

Relevant External Links for INSIG1

Genetic Association Database (GAD)
INSIG1
Human Genome Epidemiology (HuGE) Navigator
INSIG1
genes like me logo Genes that share disorders with INSIG1: view

No data available for UniProtKB/Swiss-Prot for INSIG1 Gene

Publications for INSIG1 Gene

  1. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER. (PMID: 12202038) Yang T. … Brown M.S. (Cell 2002) 3 4 23
  2. Gp78, a membrane-anchored ubiquitin ligase, associates with Insig-1 and couples sterol-regulated ubiquitination to degradation of HMG CoA reductase. (PMID: 16168377) Song B.L. … DeBose-Boyd R.A. (Mol. Cell 2005) 3 4 23
  3. Sterol-regulated ubiquitination and degradation of Insig-1 creates a convergent mechanism for feedback control of cholesterol synthesis and uptake. (PMID: 16399501) Gong Y. … Ye J. (Cell Metab. 2006) 3 4 23
  4. The INSIG1 gene, not the INSIG2 gene, associated with coronary heart disease: tagSNPs and haplotype-based association study. The Beijing Atherosclerosis Study. (PMID: 18989534) Liu X. … Gu D. (Thromb. Haemost. 2008) 3 23 48
  5. Interactions among genetic variants from SREBP2 activating-related pathway on risk of coronary heart disease in Chinese Han population. (PMID: 19740467) Liu X. … Gu D. (Atherosclerosis 2010) 3 23 48

Products for INSIG1 Gene

  • antibodies-online peptides for INSIG1
  • antibodies-online antibodies for INSIG1
  • antibodies-online kits for INSIG1

Sources for INSIG1 Gene

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