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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ING2 Gene

protein-coding   GIFtS: 54
GCID: GC04P184426

inhibitor of growth family, member 2

(Previous names: inhibitor of growth family, member 1-like )
(Previous symbol: ING1L)
 Explore 12 diseases affiliated with
ING2 via our new
 Human Malady Compendium 
Biological research products
for ING2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Inhibitor Of Growth Family, Member 21 2     ING1Lp2 3
ING1L1 2 3 5     P321
P33ING21     Inhibitor Of Growth Family, Member 1-Like1
Inhibitor Of Growth 1-Like Protein2 3     Inhibitor Of Growth Protein 22

External Ids:    HGNC: 60631   Entrez Gene: 36222   Ensembl: ENSG000001685567   OMIM: 6042155   UniProtKB: Q9H1603   

Export aliases for ING2 gene to outside databases

Previous GC identifers: GC00U990666 GC0XM068188 GC0XM068944 GC0XM069578 GC0XM070494 GC04P184802 GC04P184663 GC04P180185


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ING2:
This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate
the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair
and apoptosis. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: ING2_HUMAN, Q9H160
Function: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing
acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation
of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs)

Gene Wiki entry for ING2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NC_018915.1  NT_016354.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ING2 gene promoter:
         TBP   STAT5B   Spz1   Sp1   p300   FAC1   Nkx6-1   HSF2   ARP-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidING2 promoter sequence
   Search SABiosciences Chromatin IP Primers for ING2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ING2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q35.1   Ensembl cytogenetic band:  4q35.1   HGNC cytogenetic band: 4q35.1

ING2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ING2 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P184426:  view genomic region     (about GC identifiers)

Start:
184,426,147 bp from pter      End:
184,432,249 bp from pter
Size:
6,103 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ING2_HUMAN, Q9H160 (See protein sequence)
Recommended Name: Inhibitor of growth protein 2  
Size: 280 amino acids; 32808 Da
Subunit: Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least
consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2
Subcellular location: Nucleus. Note=Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced
PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm
Secondary accessions: O95698

Explore the universe of human proteins at neXtProt for ING2: NX_Q9H160

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9H160

  • ING2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_001555.1  
    ENSEMBL proteins: 
     ENSP00000307183   ENSP00000410291   ENSP00000412586  

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    Uscn Proteins for ING2

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0016580Sin3 complex IDA16387653
    GO:0016602CCAAT-binding factor complex IDA15243141


    ING2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ING2 for domains           About GeneDecksing

    5/6 InterPro domains/families (see all 6):
     IPR024610 ING_N
     IPR019786 Zinc_finger_PHD-type_CS
     IPR001965 Znf_PHD
     IPR013083 Znf_RING/FYVE/PHD
     IPR011011 Znf_FYVE_PHD

    Graphical View of Domain Structure for InterPro Entry Q9H160

    ProtoNet protein and cluster: Q9H160

    1 Blocks protein family: IPB001965 Zn-finger-like

    UniProtKB/Swiss-Prot: ING2_HUMAN, Q9H160
    Domain: The PHD-type zinc finger mediates the binding to H3K4me3
    Domain: The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including
    phosphatidylinositol 5-phosphate (PtdIns(5)P)
    Similarity: Belongs to the ING family
    Similarity: Contains 1 PHD-type zinc finger


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ING2_HUMAN, Q9H160
    Function: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing
    acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation
    of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs)
    Induction: Induced by the DNA-damaging agents etoposide and neocarzinostatin

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    hsa-miR-448 hsa-miR-137 hsa-miR-92a hsa-miR-586 hsa-miR-367 hsa-miR-25 hsa-miR-944 hsa-miR-32
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    Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IDA15243141
    GO:0003682chromatin binding TAS10072587
    GO:0005515protein binding IPI18334480
    GO:0008270zinc ion binding IEA--
    GO:0032403protein complex binding IDA18334480


    ING2 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for ING2:
     Decreased nuclei size in G2M 

    Animal Models:
         Mouse knock-out Ing2tm1.1Ccha for ING2
         7 MGI mutant phenotypes (inferred from 1 allele(MGI details for Ing2):
     cellular  endocrine/exocrine gland  hematopoietic system  immune system  mortality/aging 
     reproductive system  tumorigenesis 

    ING2 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Senescence and Autophagy
    Senescence and Autophagy1.00


    1 BioSystems Pathway for ING2 
        Senescence and Autophagy


    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for ING2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/97 Interacting proteins for ING2 (Q9H1602, 3 ENSP000003071834) via UniProtKB, MINT, STRING, and/or I2D (see all 97)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053, ENSP000003529804I2D: score=1 STRING: ENSP00000352980
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 5/16 biological process terms (GO ID links to tree view) (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-dependent IEA--
    GO:0006355regulation of transcription, DNA-dependent IDA15243141
    GO:0007141male meiosis I ISS--
    GO:0007165signal transduction TAS10072587
    GO:0007283spermatogenesis ISS--


    ING2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ING2
    Search CenterWatch for drugs/clinical trials and news about ING2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    About This Section

    REFSEQ mRNAs for ING2 gene: 
    NM_001564.2  

    Unigene Cluster for ING2:

    Inhibitor of growth family, member 2
    Hs.107153  [show with all ESTs]
    Unigene Representative Sequence: NM_001564
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000302327(uc003ivs.1 uc011ckk.1) ENST00000412117 ENST00000434682


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    8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate ING2 (see all 14):
    hsa-miR-448 hsa-miR-137 hsa-miR-92a hsa-miR-586 hsa-miR-367 hsa-miR-25 hsa-miR-944 hsa-miR-32
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    Additional cDNA sequence: 

    AB012853.1 AB196793.1 AF053537.1 AJ006851.1 AK294310.1 AK314000.1 BC030128.2 

    2 DOTS entries:

    DT.214433  DT.92426807 

    24/51 AceView cDNA sequences (see all 51):

    AW674790 AA312454 BF740046 N59134 BM719460 BM982877 BM929984 AF053537 
    BG741809 BC030128 CF552403 BE773086 BQ277444 NM_001564 AI080048 BG503720 
    AJ006851 BF116098 CD675497 BI460319 BE796780 BG211544 AW955130 BE772998 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ING2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGTTTCACTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ING2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ING2

    SOURCE GeneReport for Unigene cluster: Hs.107153

    UniProtKB/Swiss-Prot: ING2_HUMAN, Q9H160
    Tissue specificity: Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues

        SABiosciences Expression via Pathway-Focused PCR Array including ING2: 
              Epigenetic Chromatin Remodeling Factors in human mouse rat

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for ING2 gene from 9/26 species (see all 26)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ing21 , 5 inhibitor of growth family, member 21, 5 90.6(n)1
    96.79(a)1
      8 (26.87 cM)5
    692601  NM_023503.31  NP_075992.21 
     476671785 
    chicken
    (Gallus gallus)
    Aves ING21 inhibitor of growth family, member 2 74.74(n)
    81.75(a)
      422553  XM_420514.3  XP_420514.3 
    lizard
    (Anolis carolinensis)
    Reptilia ING26
    --
    80(a)
    1 ↔ 1
    5(113810896-113816853)
    African clawed frog
    (Xenopus laevis)
    Amphibia LOC3982662 p32INGL 74.92(n)    AY014017.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ing21 inhibitor of growth family, member 2 68.08(n)
    77.22(a)
      436721  NM_001002448.1  NP_001002448.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG73796
    MESR46
    Misexpression suppressor of ras 4
    21(a)
    2(a)
    many → 1
    possible ortholog
    3R(13622098-13624026)
    2R(13435329-13443644)
    worm
    (Caenorhabditis elegans)
    Secernentea ing-36
    Y43H11AL.16
    (see all 3)
    Protein Y43H11AL.1, isoform a
    (see all 3)
    9(a)
    7(a)
    (see all 3)
    possible ortholog
    possible ortholog
    (see all 3)
    II(13875962-13882515)
    II(222700-232741)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons ING16
    ING26
    PHD finger protein-like protein
    28(a)
    12(a)
    possible ortholog
    many → 1
    3(8675956-8678220)
    1(20304567-20307024)
    rice
    (Oryza sativa)
    Liliopsida --
    --
    PHD-finger domain containing protein, putative, ex...
    PHD-finger domain containing protein, putative, ex...
    28(a)
    18(a)
    possible ortholog
    many → 1
    3(2408857-2412428)
    3(30784503-30789908)


    ENSEMBL Gene Tree for ING2 (if available)
    TreeFam Gene Tree for ING2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ING2 gene
    ING42  ING52  ING32  ING12  
    5 SIMAP similar genes for ING2 using alignment to 3 protein entries:     ING2_HUMAN (see all proteins):
    ING2b    ING3    ING1    ING5    ING4

    ING2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section
    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ING2
    DNA2.0 Custom Variant and Variant Library Synthesis for ING2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ING2 for disorders           About GeneDecksing

    OMIM gene information: 604215    OMIM disorders: --

    12 diseases for ING2:    About MalaCards
    non-small cell lung carcinoma    soft tissue sarcoma    basal cell carcinoma    squamous cell carcinoma
    lung carcinoma    sarcoma    hepatocellular carcinoma    carcinoma
    colon cancer    lung cancer    immunodeficiency    melanoma

    1 Novoseek disease relationship for ING2 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 15.7 5 10072587 (2), 12859901 (1), 16024799 (1)


    Export disorders for ING2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ING2 gene, integrated from 9 sources (see all 42):
    (articles sorted by number of sources associating them with ING2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning of a novel gene (ING1L) homologous to ING1, a candidate tumor suppressor. (PubMed id 10072587)1, 2, 3, 9 Shimada Y.... Horie M. (1998)
    2. The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor. (PubMed id 12859901)1, 2, 9 Gozani O.... Yuan J. (2003)
    3. DNA damage-inducible gene p33ING2 negatively regulates cell proliferation through acetylation of p53. (PubMed id 11481424)1, 2, 9 Nagashima M.... Harris C.C. (2001)
    4. ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression. (PubMed id 16728974)1, 2 Shi X....Gozani O. (2006)
    5. The polybasic region that follows the plant homeodomain zinc finger 1 of Pf1 is necessary and sufficient for specific phosphoinositide binding. (PubMed id 16893883)1, 2 Kaadige M.R. and Ayer D.E. (2006)
    6. ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. (PubMed id 16387653)1, 2 Doyon Y.... Cote J. (2006)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    8. Role of the Sin3-histone deacetylase complex in growth regulation by the candidate tumor suppressor p33(ING1). (PubMed id 11784859)1, 2 Kuzmichev A.... Reinberg D. (2002)
    9. ING2 is upregulated in colon cancer and increases inv asion by enhanced MMP13 expression. (PubMed id 19437536)1, 9 Kumamoto K....Harris C.C. (2009)
    10. ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation. (PubMed id 16024799)1, 9 Pedeux R....Harris C.C. (2005)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 3622 HGNC: 6063 AceView: ING1L Ensembl:ENSG00000168556 euGenes: HUgn3622
    ECgene: ING2 H-InvDB: ING2

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