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ILK Gene

protein-coding   GIFtS: 70
GCID: GC11P006581

Integrin-Linked Kinase

  See ILK-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Integrin-Linked Kinase1 2     Epididymis Secretory Protein Li 282
59 KDa Serine/Threonine-Protein Kinase2 3     Integrin-Linked Kinase-22
ILK-12 3     Integrin-Linked Protein Kinase2
ILK-22 3     EC 2.7.11.13
p59ILK2 3     ILK13
P592 5     ILK23
HEL-S-282     

External Ids:    HGNC: 60401   Entrez Gene: 36112   Ensembl: ENSG000001663337   OMIM: 6023665   UniProtKB: Q134183   

Export aliases for ILK gene to outside databases

Previous GC identifers: GC11P007367 GC11P007085 GC11P006584 GC11P006590 GC11P006560 GC11P006283


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for ILK Gene:
This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein
associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates
integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal
transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing
results in multiple transcript variants. (provided by RefSeq, Jun 2013)

GeneCards Summary for ILK Gene:
ILK (integrin-linked kinase) is a protein-coding gene. Diseases associated with ILK include scar contracture. GO annotations related to this gene include protein serine/threonine kinase activity and signal transducer activity. An important paralog of this gene is FPGT-TNNI3K.

UniProtKB/Swiss-Prot: ILK_HUMAN, Q13418
Function: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator
of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is
considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be
implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in
epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also
AKT1 and GSK3B

Gene Wiki entry for ILK (Integrin-linked kinase) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the ILK gene promoter:
         ATF-2   STAT3   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidILK promoter sequence
   Search Chromatin IP Primers for ILK

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ILK


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p15.4   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15.4

ILK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ILK gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P006581:  view genomic region     (about GC identifiers)

Start:
6,624,961 bp from pter      End:
6,632,102 bp from pter
Size:
7,142 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ILK_HUMAN, Q13418 (See protein sequence)
Recommended Name: Integrin-linked protein kinase  
Size: 452 amino acids; 51419 Da
Subunit: Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also
interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts
with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts
(via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of
EPHA1
Sequence caution: Sequence=CAB94832.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact. Was
originally thought to be distinct gene (ILK2) (PubMed:10871859);
Selected PDB 3D structures from and Proteopedia for ILK (see all 8):
2KBX (3D)        3F6Q (3D)        3IXE (3D)        3KMU (3D)        3KMW (3D)        3REP (3D)    
Secondary accessions: D3DQU0 P57043 Q68DZ3

Explore the universe of human proteins at neXtProt for ILK: NX_Q13418

Explore proteomics data for ILK at MOPED

Post-translational modifications: 

  • Autophosphorylated on serine residues1
  • Ubiquitination2 at Lys85, Lys165, Lys191, Lys220
  • Modification sites at PhosphoSitePlus

  • See ILK Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (5 alternative transcripts): 
    NP_001014794.1  NP_001014795.1  NP_001265370.1  NP_001265371.1  NP_004508.1  

    ENSEMBL proteins: 
     ENSP00000299421   ENSP00000434492   ENSP00000432796   ENSP00000403487   ENSP00000432617  
     ENSP00000433129   ENSP00000436062   ENSP00000435323   ENSP00000379975   ENSP00000439606  
    Reactome Protein details: Q13418

    ILK Human Recombinant Protein Products:

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    Novus Biologicals ILK Proteins
    Novus Biologicals ILK Lysates
    Browse Sino Biological Recombinant Proteins
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    ProSpec Recombinant Protein for ILK
    Cloud-Clone Corp. Proteins for ILK

     
    Search eBioscience for Proteins for ILK 

    ILK Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of ILK
    R&D Systems Antibodies for ILK
    Cell Signaling Technology (CST) Antibodies for ILK 
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    Novus Biologicals ILK Antibodies
    Abcam antibodies for ILK
    Cloud-Clone Corp. Antibodies for ILK
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    Search LSBio for Antibodies for ILK

    ILK Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ILK
    Cloud-Clone Corp. CLIAs for ILK
    Search eBioscience for ELISAs for ILK 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ANKRD: Ankyrin repeat domain containing

    IUPHAR Guide to PHARMACOLOGY protein family classification: integrin-linked kinase
    ILK subfamily

    Selected InterPro protein domains (see all 6):
     IPR016253 Integrin-linked_kinase
     IPR011009 Kinase-like_dom
     IPR000719 Prot_kinase_dom
     IPR002110 Ankyrin_rpt
     IPR001245 Ser-Thr/Tyr_kinase_cat_dom

    Graphical View of Domain Structure for InterPro Entry Q13418

    ProtoNet protein and cluster: Q13418

    1 Blocks protein domain: IPB002110 Ankyrin repeat signature

    UniProtKB/Swiss-Prot: ILK_HUMAN, Q13418
    Domain: A PH-like domain is involved in phosphatidylinositol phosphate binding
    Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family
    Similarity: Contains 5 ANK repeats
    Similarity: Contains 1 protein kinase domain


    Find genes that share domains with ILK           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ILK_HUMAN, Q13418
    Function: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator
    of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is
    considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be
    implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in
    epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also
    AKT1 and GSK3B
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by
    insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK

         Genatlas biochemistry entry for ILK:
    integrin-linked kinase,59kDa,widely expressed,potential regulator of integrin beta-1 mediated signal
    transduction,overlapping TAF2H by its 3'end (divergently oriented)

         Enzyme Number (IUBMB): EC 2.7.11.11

         Gene Ontology (GO): Selected molecular function terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IDA10871859
    GO:0004713protein tyrosine kinase activity ----
    GO:0004871signal transducer activity IEA--
    GO:0005178integrin binding IEA--
         
    Find genes that share ontologies with ILK           About GenesLikeMe


    Phenotypes:
         6 GenomeRNAi human phenotypes for ILK:
     Decreased substrate adherent c  Increased cell death HMECs cel  Increased mitotic index  Increases Tamoxifen sensitivit 
     Synthetic lethal with Ras  Synthetic lethal with paclitax 

         Selected MGI mutant phenotypes (inferred from 12 alleles(MGI details for Ilk) (see all 19):
     behavior/neurological  cardiovascular system  cellular  craniofacial  digestive/alimentary 
     embryogenesis  growth/size/body  hematopoietic system  homeostasis/metabolism  immune system 
     integument  mortality/aging  no phenotypic analysis  normal  other 

    Find genes that share phenotypes with ILK           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for ILK: Ilktm2Ref Ilktm2Star

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ILK
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ILK

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ILK
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ILK

    miRNA
    Products:
        
    miRTarBase miRNAs that target ILK:
    hsa-mir-542-3p (MIRT007009), hsa-mir-145-5p (MIRT007180), hsa-mir-625-5p (MIRT006720), hsa-mir-744-5p (MIRT037481), hsa-mir-222-3p (MIRT046790)

    Block miRNA regulation of human, mouse, rat ILK using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate ILK:
    hsa-miR-2116* hsa-miR-542-3p hsa-miR-624* hsa-miR-758
    SwitchGear 3'UTR luciferase reporter plasmidILK 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ILK
    Predesigned siRNA for gene silencing in human, mouse, rat ILK

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ILK

    Clone
    Products:
         
    OriGene clones in human, mouse for ILK (see all 17)
    OriGene ORF clones in mouse, rat for ILK
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): ILK (NM_004517)
    Sino Biological Human cDNA Clone for ILK
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ILK
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ILK
    Addgene plasmids for ILK 

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for ILK
    Browse ESI BIO Cell Lines and PureStem Progenitors for ILK 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ILK


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ILK_HUMAN, Q13418: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
    Cell projection, lamellipodium (By similarity). Cytoplasm, myofibril, sarcomere
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    cytosol4
    cytoskeleton3
    extracellular2
    mitochondrion2
    nucleus2
    peroxisome1

    Gene Ontology (GO): Selected cellular component terms (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005886plasma membrane IEA--

    Find genes that share ontologies with ILK           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ILK About   (see all 15)  
    See pathways by source

    SuperPathContained pathways About
    1Focal adhesion
    Focal adhesion0.65
    Integrin-mediated cell adhesion0.38
    Focal Adhesion0.65
    2Endometrial cancer
    Endometrial cancer0.45
    Signal transduction PTEN pathway0.45
    3Cell junction organization
    Cell junction organization0.70
    Cell-extracellular matrix interactions0.00
    Cell-Cell communication0.64
    Localization of the PINCH-ILK-PARVIN complex to focal adhesions0.00
    4Sertoli-Sertoli Cell Junction Dynamics
    Sertoli-Sertoli Cell Junction Dynamics0.38
    Epithelial Tight Junctions0.36
    Germ Cell-Sertoli Cell Junction Dynamics0.38
    5Akt Signaling
    Akt Signaling0.60


    Find genes that share SuperPaths with ILK           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    5 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for ILK
        Sertoli-Sertoli Cell Junction Dynamics
    ILK Signaling
    Epithelial Tight Junctions
    Akt Signaling
    Germ Cell-Sertoli Cell Junction Dynamics

    1 Cell Signaling Technology (CST) Pathway for ILK
        Adhesion

    5 GeneGo (Thomson Reuters) Pathways for ILK
        Cytoskeleton remodeling Integrin outside-in signaling
    Cell adhesion PLAU signaling
    Development EGFR signaling pathway
    Cytoskeleton remodeling Reverse signaling by ephrin B
    Signal transduction PTEN pathway

    5 BioSystems Pathways for ILK
        Focal Adhesion
    Integrin-mediated cell adhesion
    Eukaryotic Transcription Initiation
    Integrins in angiogenesis
    Osteopontin-mediated events


    2 Reactome Pathways for ILK
        Localization of the PINCH-ILK-PARVIN complex to focal adhesions
    Cell-extracellular matrix interactions


    4 Kegg Pathways  (Kegg details for ILK):
        PPAR signaling pathway
    Focal adhesion
    Bacterial invasion of epithelial cells
    Endometrial cancer

        Pathway & Disease-focused RT2 Profiler PCR Arrays including ILK (see all 8): 
              mTOR Signaling in human mouse rat
              Fibrosis in human mouse rat
              Tight Junctions in human mouse rat
              Cell Motility in human mouse rat
              PI3K-AKT Signaling Pathway in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for ILK

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ILK (Q134181, 2, 3 ENSP000002994214) via UniProtKB, MINT, STRING, and/or I2D (see all 706)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CASP8Q147901, 3, ENSP000003512734EBI-747644,EBI-78060 I2D: score=3 STRING: ENSP00000351273
    CASP9P552111, 3, ENSP000003302374EBI-747644,EBI-516799 I2D: score=3 STRING: ENSP00000330237
    CKBP122772, 3, ENSP000002991984MINT-8258588 I2D: score=2 STRING: ENSP00000299198
    COPG1Q9Y6782, 3, ENSP000003250024MINT-7945693 I2D: score=2 STRING: ENSP00000325002
    FANCIQ9NVI12, 3, ENSP000003108424MINT-7945693 I2D: score=2 STRING: ENSP00000310842
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 44):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001558regulation of cell growth ----
    GO:0001658branching involved in ureteric bud morphogenesis IEA--
    GO:0001954positive regulation of cell-matrix adhesion IEA--
    GO:0006468protein phosphorylation IDA10871859
    GO:0006469negative regulation of protein kinase activity IEA--

    Find genes that share ontologies with ILK           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ILK

    2 HMDB Compounds for ILK    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--

    Selected Novoseek inferred chemical compound relationships for ILK gene (see all 26)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    glycogen 60.8 28 12778079 (2), 15735038 (2), 15867245 (2), 16648553 (2) (see all 23)
    phosphatidylinositol 58.4 16 16546979 (1), 17107957 (1), 11313365 (1), 16407822 (1) (see all 15)
    threonine 55.9 23 15722199 (2), 11694518 (1), 17656471 (1), 19262169 (1) (see all 22)
    phosphoinositide 51 13 12101188 (4), 17298394 (1), 19920184 (1), 12637511 (1) (see all 9)
    ly294002 50.4 6 15044083 (2), 17234779 (1), 14751515 (1), 16472257 (1)
    serine 43 23 15722199 (2), 11694518 (1), 17656471 (1), 19262169 (1) (see all 22)
    rapamycin 39 14 16014356 (1), 17588951 (1), 20232298 (1), 16397254 (1) (see all 8)
    chlorothalonil 35.6 3 2266108 (1)
    matrigel 33.9 3 12925691 (1), 16818815 (1)
    wortmannin 33 2 15044083 (1), 14751515 (1)



    Find genes that share compounds with ILK           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for ILK gene (5 alternative transcripts): 
    NM_001014794.2  NM_001014795.2  NM_001278441.1  NM_001278442.1  NM_004517.3  

    17 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000299421(uc001mee.3 uc001mef.3 uc010raq.2) ENST00000532063
    ENST00000527327 ENST00000420936 ENST00000526114 ENST00000527394 ENST00000527121
    ENST00000528995 ENST00000534565 ENST00000396751(uc001meh.3) ENST00000526318
    ENST00000524735 ENST00000528784 ENST00000534706 ENST00000530016 ENST00000526711
    ENST00000537806(uc010rap.2)
    miRNA
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    4 qRT-PCR Assays for microRNAs that regulate ILK:
    hsa-miR-2116* hsa-miR-542-3p hsa-miR-624* hsa-miR-758
    SwitchGear 3'UTR luciferase reporter plasmidILK 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ILK
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    GenScript: all cDNA clones in your preferred vector (see all 3): ILK (NM_004517)
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    Addgene plasmids for ILK 
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    OriGene qSTAR qPCR primer pairs in human, mouse for ILK
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ILK
      QuantiTect SYBR Green Assays in human, mouse, rat ILK
      QuantiFast Probe-based Assays in human, mouse, rat ILK

    Selected AceView cDNA sequences (see all 522):

    BQ084124 CA976008 CA306218 CN482888 BU783009 AI493783 CN482245 CR604127 
    BG682011 CB141333 BM705185 BQ774612 BM475390 BM765458 BU500956 BQ931548 
    BX363152 BQ721237 CR615121 BG983192 BQ222152 CR611887 CA309508 AI348148 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ILK (see all 16)    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11a · 11b · 11c ^ 12a · 12b · 12c ^ 13 ^
    SP1:                                -     -           -                             -                 -                                                         
    SP2:        -     -                 -     -           -                             -                 -                                                         
    SP3:        -                             -           -                             -                 -                                                         
    SP4:        -     -                 -     -           -                                               -                                                         
    SP5:        -                       -     -           -                             -                 -                                                         

    ExUns: 14a · 14b ^ 15
    SP1:                  
    SP2:                  
    SP3:                  
    SP4:                  
    SP5:                  


    ECgene alternative splicing isoforms for ILK

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ILK expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GGAGGGATCA
    ILK Expression
    About this image


    ILK expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 16) fully expand
     
     Eye (Sensory Organs)    fully expand to see all 2 entries
             Lens Vesicle Cells Lens Vesicle
     
     Epithelial Cells
             Anterior Lens Epithelial Cells Lens
     
     Colon (Gastrointestinal Tract)
     
     Uterus (Reproductive System)
     
     Thyroid (Endocrine System)
    ILK Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ILK Protein Expression

    UniProtKB/Swiss-Prot: ILK_HUMAN, Q13418
    Tissue specificity: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed
    in placenta, lung and liver

        Pathway & Disease-focused RT2 Profiler PCR Arrays including ILK (see all 8): 
              mTOR Signaling in human mouse rat
              Fibrosis in human mouse rat
              Tight Junctions in human mouse rat
              Cell Motility in human mouse rat
              PI3K-AKT Signaling Pathway in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

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    This gene was present in the common ancestor of animals.

    Orthologs for ILK gene from Selected species (see all 18)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ilk1 , 5 integrin linked kinase1, 5 91.81(n)1
    99.56(a)1
      7 (55.97 cM)5
    162021  NM_001161724.11  NP_001155196.11 
     1057365925 
    chicken
    (Gallus gallus)
    Aves ILK1 integrin-linked kinase 80.31(n)
    90.71(a)
      374018  NM_204194.1  NP_989525.1 
    lizard
    (Anolis carolinensis)
    Reptilia ILK6
    integrin-linked kinase
    73(a)
    1 ↔ 1
    GL345104.1(67-10590)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.59782 Xenopus laevis transcribed sequence with moderate similarity more 78.15(n)    BU916167.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc658422 hypothetical protein MGC65842 79.11(n)   393543  BC056593.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Ilk1 , 3 integrin-mediated signaling pathway
    protein serine/threonine more3
    Integrin linked kinase1
    59(a)3
    60.48(n)1
    60.63(a)1
      78C23
    535731  NM_080262.41  NP_525001.21 
    worm
    (Caenorhabditis elegans)
    Secernentea pat-41 , 3 integrin-linked kinase3
    pat-41
    58(a)3
    57.85(n)1
    58.97(a)1
      III(89525-91902)3
    1751751  NM_064738.51  NP_497139.11 


    ENSEMBL Gene Tree for ILK (if available)
    TreeFam Gene Tree for ILK (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ILK gene
    FPGT-TNNI3K2  MAP3K122  MAP3K112  KIAA18042  RAF12  KSR22  KSR12  MOS2  
    BRAF2  MAP3K92  MAP3K102  ARAF2  MAP3K72  TNNI3K2  ZAK2  MAP3K132  
    10 SIMAP similar genes for ILK using alignment to 7 protein entries:     ILK_HUMAN (see all proteins):
    PLA2G6    ANKRD42    ANKRD44    ANKRD54    HACE1    RAI14
    GABPB1    IkBa    ANKRD65    tec

    Find genes that share paralogs with ILK           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

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    Selected SNPs for ILK (see all 320)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs110409341,2
    C,F,H--6537283(+) CTGGGC/GTGAAT 2 -- us2k110Minor allele frequency- G:0.03NS EA NA WA 5006
    rs2001722761,2
    --6537335(+) TCTTCA/GGCAGA 2 -- us2k10--------
    rs2014542601,2
    --6537398(+) TCCTCA/GGAGTC 3 -- us2k10--------
    rs773253061,2
    C--6537449(+) TTCTTC/TCCTAC 3 -- us2k10--------
    rs1443257981,2
    C,F--6537487(+) CTTTTT/GGGGGC 3 -- us2k11Minor allele frequency- G:0.00NA 4550
    rs2000967441,2
    C--6537492(+) TGGGGA/CATTTC 3 -- us2k10--------
    rs1904618451,2
    C--6537543(+) ATAGGC/GTGGGG 3 -- us2k10--------
    rs2019514451,2
    C--6537547(+) GCTGGA/GGGGAT 3 -- us2k10--------
    rs2021466581,2
    --6537581(+) AGGGCC/TCGTAA 3 -- us2k10--------
    rs1825586561,2
    C--6537583(+) GGCCCC/GTAATG 3 -- us2k10--------

    HapMap Linkage Disequilibrium report for ILK (6624961 - 6632102 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for ILK:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv996362CNV Deletion20482838
    nsv896972CNV Loss21882294
    nsv896971CNV Loss21882294
    nsv825755CNV Gain20364138

    Human Gene Mutation Database (HGMD): ILK
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ILK
    DNA2.0 Custom Variant and Variant Library Synthesis for ILK

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

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    OMIM gene information: 602366    OMIM disorders: --

    1 disease for ILK:    
    About MalaCards
    scar contracture

    1 disease from the University of Copenhagen DISEASES database for ILK:
    Cancer

    Find genes that share disorders with ILK           About GenesLikeMe

    Selected Novoseek inferred disease relationships for ILK gene (see all 43)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    renal fibrosis 49.7 4 12925691 (2), 17498677 (2)
    carcinoma non-small cell lung 34.4 1 19176380 (1)
    tumors 32.8 94 17224516 (5), 19923885 (5), 12596061 (4), 15631637 (3) (see all 39)
    cancer 30.9 55 19895269 (3), 17143519 (3), 18505933 (3), 19409087 (2) (see all 27)
    ovarian cancer 29.9 20 14517840 (4), 17187779 (3), 12642872 (2), 19334037 (2) (see all 8)
    metastasis 29.4 18 12596061 (3), 16836071 (3), 15300800 (2), 15631637 (1) (see all 9)
    familial adenomatous polyposis 28.9 2 16799642 (1), 12771992 (1)
    thyroid carcinoma anaplastic 28.4 6 16093430 (5)
    prostate carcinoma 27.8 4 14990992 (1), 9681506 (1), 10716737 (1)
    prostate cancer 25.4 12 15704679 (4), 15870868 (1), 15782140 (1), 10716737 (1) (see all 6)

    Genetic Association Database (GAD): ILK
    Human Genome Epidemiology (HuGE) Navigator: ILK (1 document)

    Export disorders for ILK gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ILK gene, integrated from 10 sources (see all 375):
    (articles sorted by number of sources associating them with ILK)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. (PubMed id 8538749)1, 2, 3 Hannigan G.E.... Dedhar S. (Nature 1996)
    2. Cloning of an isoform of integrin-linked kinase (ILK) that is upregulated in HT-144 melanoma cells following TGF-beta1 stimulation. (PubMed id 10871859)1, 2, 9 Janji B.... Kieffer N. (Oncogene 2000)
    3. Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. (PubMed id 9736715)1, 2, 9 Delcommenne M.... Dedhar S. (Proc. Natl. Acad. Sci. U.S.A. 1998)
    4. Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase. (PubMed id 19117955)1, 2, 9 Yang Y.... Qin J. (J. Biol. Chem. 2009)
    5. The pseudoactive site of ILK is essential for its binding to alpha- parvin and localization to focal adhesions. (PubMed id 20005845)1, 2, 9 Fukuda K.... Qin J. (Mol. Cell 2009)
    6. Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival. (PubMed id 15284246)1, 2, 9 Zhang Y.... Wu C. (J. Biol. Chem. 2004)
    7. A novel integrin-linked kinase-binding protein, affixin, is involved in the early stage of cell-substrate interaction. (PubMed id 11402068)1, 2, 9 Yamaji S.... Ishigatsubo Y. (J. Cell Biol. 2001)
    8. The structural basis of integrin-linked kinase-PINCH interactions. (PubMed id 19074270)1, 2, 9 Chiswell B.P.... Calderwood D.A. (Proc. Natl. Acad. Sci. U.S.A. 2008)
    9. Laminin-alpha4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and endothelial cells. (PubMed id 17646580)1, 2, 9 KnAPll R....Bakkers J. (Circulation 2007)
    10. EphA1 interacts with integrin-linked kinase and regulates cell morphology and motility. (PubMed id 19118217)1, 2, 9 Yamazaki T.... Maru Y. (J. Cell Sci. 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 3611 HGNC: 6040 AceView: ILK Ensembl:ENSG00000166333 euGenes: HUgn3611
    ECgene: ILK Kegg: 3611 H-InvDB: ILK

    (According to HUGE)
    About This Section

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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for ILK Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for ILK Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for ILK gene:
    Search GeneIP for patents involving ILK

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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