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IFNA8 Gene

protein-coding GIFtS: 57

GC09P021399

interferon, alpha 8
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
OTTHUMP00000021146 ^²

Descriptions
Interferon alpha-B ^³
Interferon alpha-B2 ^³
LeIF B ^³
interferon alpha type 201 ^²
interferon alpha-B' ^²
interferon, alpha 8 ^²

External Ids
HGNC: 5429¹
Entrez Gene: 3445²
UniProtKB: P32881³
Ensembl: ENSG00000120242⁷

Search outside databases for aliases for IFNA8 gene

Previous GC identifers: GC09P021736 GC09P021610

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: IFNA8_HUMAN, P32881

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Gene Wiki entry for IFNA8

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the IFNA8 gene

Entrez Gene cytogenetic band: 9p22 Ensembl cytogenetic band: 9p21.3 HGNC cytogenetic band: 9p22

IFNA8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09P021399: (about GC identifiers)

Start:	21,399,146 bp from pter
End:	21,400,184 bp from pter
Size:	1,039 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000009.10 NT_008413.17

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: IFNA8_HUMAN, P32881 (See protein sequence)

Recommended Name: Interferon alpha-8 precursor

Size: 189 amino acids; 21989 Da

Subcellular location: Secreted

Secondary accessions: P01565 P09236

REFSEQ proteins: NP_002161.2

ENSEMBL proteins:

ENSP00000369553

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	R&D Systems Recombinant & Natural Proteins for IFNA8 (IFN-alpha B2)
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (Interferon alpha 8)
	Human Recombinant Proteins from Abnova (IFNA8)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--
GO:0005615	extracellular space	IEA	--

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Antibodies for IFNA8:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (Interferon alpha 8), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (IFNA8)
	Novus Biologicals Antibodies for IFNA8

Assays for IFNA8:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry P32881

ProtoNet protein and cluster: P32881

1 Blocks protein family: IPB000471 Interferon alpha

UniProtKB/Swiss-Prot: IFNA8_HUMAN, P32881

Similarity: Belongs to the alpha/beta interferon family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (IFNA8)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (IFNA8)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002170

Applied Biosystems Silencer® siRNAs for IFNA8

Sigma-Aldrich siRNA for IFNA8

Sigma-Aldrich shRNA Panels and shRNA for IFNA8

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for IFNA8
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_002170
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002170
untagged cDNA clone in CMV expression vector: NM_002170

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_002170

UniProtKB/Swiss-Prot: IFNA8_HUMAN, P32881

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Genatlas biochemistry entry for IFNA8:

interferon type I,alpha 8,leukocyte

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005125	cytokine activity	IEA	--
GO:0005126	hematopoietin/interferon-class (D200-domain) cytokine receptor binding	TAS	6163083

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

4 Sigma-Aldrich "Your Favorite Gene" Pathways for IFNA8 (Your Favorite Gene powered by Ingenuity)

Role of RIG1-like Receptors in Antiviral Innate Immunity
Role of Cytokines in Mediating Communication between Immune Cells
Activation of IRF by Cytosolic Pattern Recognition Receptors
IL-12 Signaling and Production in Macrophages

GeneDecks IFNA8 for the pathways selected above

About GeneDecksing

5/29 Interacting proteins for IFNA8 (ENSP00000369553³) via UniProtKB, MINT, and/or STRING (see all 29 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
IFNAR2	ENSP00000371676³	STRING (score=.996)
IFNG	ENSP00000229135³	STRING (score=.964)
IFNA17	ENSP00000369562³	STRING (score=.901)
IFNA5	ENSP00000369560³	STRING (score=.901)
IFNA14	ENSP00000369569³	STRING (score=.9)

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2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006952	defense response	IEA	--
GO:0009615	response to virus	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for IFNA8

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (IFNA8)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (IFNA8)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002170

Sigma-Aldrich siRNA for IFNA8

Sigma-Aldrich shRNA Panels and shRNA for IFNA8

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_002170

REFSEQ mRNAs for IFNA8 gene:

NM_002170.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_002170

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002170
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002170
                                 untagged cDNA clone in CMV expression vector: NM_002170

Additional cDNA sequence:

BC104828.1 BC104830.1 K01900.1 V00545.1 V00550.1

1 DOTS entry:

DT.208852

4 AceView cDNA sequences:

V00550 V00545 K01900 NM_002170

highest scoring ESTs for IFNA8:

K01900 V00545 V00550 NM_002170 BC104828 BC104830 J00208

Unigene Cluster for IFNA8:

Interferon, alpha 8

Hs.73890

Unigene Representative Sequence: K01900

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000380205

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

IFNA8 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for IFNA8

¹ / ² / ³

3 probe-sets matching IFNA8 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
682_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	K01900	1.00	1.00	1.00	1
207932_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_002170	0.60	1.00	0.82	1
207932_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.73890
Expression variation in blood from EXPOLDB for IFNA8

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for IFNA8 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	IFNA8¹	--	interferon, alpha 8	98.94(n) 98.41(a)	473199 XM_528569.2 XP_528569.2

About this table Species with no ortholog for IFNA8

ENSEMBL Gene Tree for IFNA8

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for IFNA8 gene

IFNA1² IFNA21² IFNA2² IFNA7² IFNA17² IFNA4² IFNA6² IFNA14²

IFNA13² IFNA16² IFNA10² IFNA5²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/39 NCBI SNPs in IFNA8 are shown (see all 39 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 45)

AB	SNP ID	Valid	Chr 9 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs1330322^1,2	A,C,F,H,O	21397250(-)	`ATTGCG/ATGTTA`	1	--	ng5¹		8	EU EA WA NA	636
	rs4978116^1,2	A,C,F,H	21397638(+)	`TTTAGG/TTATAC`	1	--	ng5¹		4	EU EA WA	416
	rs4978115^1,2	A,C,F,H	21397604(+)	`GGGATA/GTTACT`	1	--	ng5¹		5	NS EU EA WA	1772
	rs10811537^1,2	A,C,F,H	21398824(+)	`TTGTTT/CTCTAA`	1	--	ng5¹		5	EU EA WA NS	1896
	rs3739630^1,2	C,F,H	21399584(-)	`GTCCTC/TGTACA`	1	E/K	mis¹		12	EA NA EU WA	2464
	rs10811535^1,2	A,F,H	21397696(+)	`CTTTTG/TCTTTC`	1	--	ng5¹		4	EU EA WA	418
	rs10964981^1,2	C,F,H	21398292(+)	`GCTTAT/AGTTTG`	1	--	ng5¹		8	EU EA WA	836
	rs12553612^1,2	C,F,H	21398857(+)	`attcaA/Catatg`	1	--	ng5¹		8	EU EA WA	840
	rs10964982^1,2	C,F,H	21399901(+)	`ATCCTG/ATTCAG`	1	--	ut3¹		7	EU EA WA NA	556
	rs1330321^1,2	C,F,H	21397375(-)	`AAACAT/CAAGAC`	1	--	ng5¹		8	EU EA WA	836

About this table

HapMap Linkage Disequilibrium images for IFNA8 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 147568

Human Genome Epidemiology Navigator: IFNA8 (2 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/18 PubMed articles for IFNA8 gene (see all 18 ):

Interferon-alpha 8b is the only variant of interferon-alpha 8 identified in a large human population. (PubMed id 8836918)^{1, 3, 4} Hussain M.... Testa D. (1996)
Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes. (PubMed id 9425112)^{3, 4} Nyman T.A.... Kalkkinen N. (1998)
Mapping of the shortest region of overlap of deletions of the short arm of chromosome 9 associated with human neoplasia. (PubMed id 1385305)^{2, 3} Olopade O.I....Diaz M.O. (1992)
Structural relationship of human interferon alpha genes and pseudogenes. (PubMed id 4057246)^{3, 4} Henco K.... Weissmann C. (1985)
Cloning of eukaryotic genes in single-strand phage vectors: the human interferon genes. (PubMed id 6325303)^{3, 4} Bowden D.W....Vovis G.F. (1984)
The structure of eight distinct cloned human leukocyte interferon cDNAs. (PubMed id 6163083)^{3, 4} Goeddel D.V.... Gray P.W. (1981)
Bacterial synthesis of a novel human leukocyte interferon. (PubMed id 6164048)^{3, 4} Yelverton E.... Goeddel D.V. (1981)
Genetic susceptibility to respiratory syncytial virus bronchiolitis in preterm children is associated with airway remodeling genes and innate immune genes. (PubMed id 19258923)³ Siezen C.L....Janssen R. (2009)
A structural basis for interferon-alpha-receptor interactions. (PubMed id 17517919)³ Kumaran J....Fish E.N. (2007)
Genetic susceptibility to respiratory syncytial virus bronchiolitis is predominantly associated with innate immune genes. (PubMed id 17703412)³ Janssen R....Hoebee B. (2007)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3445	HGNC: 5429	AceView: IFNA8	Ensembl:ENSG00000120242	euGenes: HUgn3445
ECgene: IFNA8	H-InvDB: IFNA8

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for IFNA8
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for IFNA8:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for IFNA8-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for IFNA8		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for IFNA8		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (IFNA8)
	Antibodies (IFNA8)
	Chimera RNAi (IFNA8)
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

Search for Antibodies & Assays

	Recombinant Proteins (Interferon alpha 8)
	Antibodies (Interferon alpha 8)

Search OriGene for IFNA8

Search Tocris compounds for IFNA8

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for IFNA8

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IFNA8 Gene

protein-coding GIFtS: 57

Aliases &
Descriptions
for IFNA8

Summaries
for IFNA8

Genomic Location
for IFNA8

Proteins
for IFNA8

Protein Domains/
Families
for IFNA8

Gene Function
for IFNA8

Pathways & Interactions
for IFNA8

Drugs & Compounds
for IFNA8

Transcripts
for IFNA8

Expression
for IFNA8

Orthologs
for IFNA8

Paralogs
for IFNA8

SNPs/Variants
for IFNA8

Disorders & Mutations
for IFNA8

Medical News
for IFNA8

Publications
for IFNA8

Search for IFNA8

Genome Databases showing IFNA8

Other Databases showing IFNA8

Specialized Databases showing IFNA8

Licensable Technologies
for IFNA8

Services
for IFNA8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

IFNA8 Gene

protein-coding GIFtS: 57

Aliases & Descriptionsfor IFNA8

Summariesfor IFNA8

Genomic Locationfor IFNA8

Proteinsfor IFNA8

Protein Domains/Familiesfor IFNA8

Gene Functionfor IFNA8

Pathways & Interactionsfor IFNA8

Drugs & Compoundsfor IFNA8

Transcriptsfor IFNA8

Expression for IFNA8

Orthologsfor IFNA8

Paralogsfor IFNA8

SNPs/Variantsfor IFNA8

Disorders & Mutationsfor IFNA8

Medical Newsfor IFNA8

Publicationsfor IFNA8

Search for IFNA8

Genome Databases showing IFNA8

Other Databases showing IFNA8

Specialized Databases showing IFNA8

Licensable Technologiesfor IFNA8

Servicesfor IFNA8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for IFNA8

Summaries
for IFNA8

Genomic Location
for IFNA8

Proteins
for IFNA8

Protein Domains/
Families
for IFNA8

Gene Function
for IFNA8

Pathways & Interactions
for IFNA8

Drugs & Compounds
for IFNA8

Transcripts
for IFNA8

Expression
for IFNA8

Orthologs
for IFNA8

Paralogs
for IFNA8

SNPs/Variants
for IFNA8

Disorders & Mutations
for IFNA8

Medical News
for IFNA8

Publications
for IFNA8

Licensable Technologies
for IFNA8

Services
for IFNA8