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IFNA5 Gene

protein-coding GIFtS: 59

GC09M021294

interferon, alpha 5
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
INFA5 ^²
OTTHUMP00000021138 ^²

Descriptions
Interferon alpha-61 ^³
Interferon alpha-G ^³
LeIF G ^³
interferon, alpha 5 ^²

External Ids
HGNC: 5426¹
Entrez Gene: 3442²
UniProtKB: P01569³
Ensembl: ENSG00000147873⁷

Search outside databases for aliases for IFNA5 gene

Previous GC identifers: GC09M021632 GC09M021505

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: IFNA5_HUMAN, P01569

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Gene Wiki entry for IFNA5

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the IFNA5 gene

Entrez Gene cytogenetic band: 9p22 Ensembl cytogenetic band: 9p21.3 HGNC cytogenetic band: 9p22

IFNA5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09M021294: (about GC identifiers)

Start:	21,294,325 bp from pter
End:	21,295,311 bp from pter
Size:	987 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000009.10 NT_008413.17

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: IFNA5_HUMAN, P01569 (See protein sequence)

Recommended Name: Interferon alpha-5 precursor

Size: 189 amino acids; 21942 Da

Subcellular location: Secreted

Secondary accessions: Q52LX3

REFSEQ proteins: NP_002160.1

ENSEMBL proteins:

ENSP00000369560

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	R&D Systems Recombinant & Natural Proteins for IFNA5 (IFN-alpha G)
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (IFNA5)
	Human Recombinant Proteins from Abnova (IFNA5)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--
GO:0005615	extracellular space	IEA	--

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Antibodies for IFNA5:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (IFNA5), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (IFNA5)
	Novus Biologicals Antibodies for IFNA5

Assays for IFNA5:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry P01569

ProtoNet protein and cluster: P01569

1 Blocks protein family: IPB000471 Interferon alpha

UniProtKB/Swiss-Prot: IFNA5_HUMAN, P01569

Similarity: Belongs to the alpha/beta interferon family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (IFNA5)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002169

Applied Biosystems Silencer® siRNAs for IFNA5

Sigma-Aldrich siRNA and siRNA Panels for IFNA5

Sigma-Aldrich shRNA Panels and shRNA for IFNA5

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for IFNA5
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_002169
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002169
untagged cDNA clone in CMV expression vector: NM_002169

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_002169

UniProtKB/Swiss-Prot: IFNA5_HUMAN, P01569

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Genatlas biochemistry entry for IFNA5:

interferon type I,alpha 5,leukocyte

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005125	cytokine activity	IEA	--
GO:0005126	hematopoietin/interferon-class (D200-domain) cytokine receptor binding	TAS	6163083

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

5/8 Sigma-Aldrich "Your Favorite Gene" Pathways for IFNA5 (Your Favorite Gene powered by Ingenuity) (see all 8 )

Role of Cytokines in Mediating Communication between Immune Cells
Dendritic Cell Maturation
Interferon Signaling
Activation of IRF by Cytosolic Pattern Recognition Receptors
Role of RIG1-like Receptors in Antiviral Innate Immunity

GeneDecks IFNA5 for the pathways selected above

About GeneDecksing

5/59 Interacting proteins for IFNA5 (ENSP00000369560³) via UniProtKB, MINT, and/or STRING (see all 59 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
IFNG	ENSP00000229135³	STRING (score=.997)
IFNAR2	ENSP00000371676³	STRING (score=.996)
STAT2	ENSP00000315768³	STRING (score=.96)
IRF3	ENSP00000310127³	STRING (score=.929)
RNF31	ENSP00000315112³	STRING (score=.928)

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2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006952	defense response	IEA	--
GO:0009615	response to virus	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for IFNA5

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (IFNA5)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002169

Sigma-Aldrich siRNA and siRNA Panels for IFNA5

Sigma-Aldrich shRNA Panels and shRNA for IFNA5

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_002169

REFSEQ mRNAs for IFNA5 gene:

NM_002169.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_002169

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002169
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002169
                                 untagged cDNA clone in CMV expression vector: NM_002169

Additional cDNA sequence:

BC093755.1 BC093757.1 V00541.1

1 DOTS entry:

DT.304672

1 AceView cDNA sequence:

V00541

highest scoring ESTs for IFNA5:

V00541 BC093755 BC093757 NM_002169 J00213 X02956

Unigene Cluster for IFNA5:

Interferon, alpha 5

Hs.37113

Unigene Representative Sequence: V00541

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000380212

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

IFNA5 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for IFNA5

¹ / ² / ³

4 probe-sets matching IFNA5 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
32415_at²^{, 3}	U95-A	8	1.00	0.74	-0.01	0.76	V00541	1.00	1.00	1.00	1
1540_f_at²^{, 3}	U95-A	15	1.00	0.46	1.00	1.00	X02956	0.20	1.00	0.72	1
214569_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_002169	0.60	1.00	0.82	1
214569_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.37113
Expression variation in blood from EXPOLDB for IFNA5

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for IFNA5 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	IFNA5¹	--	interferon, alpha 5	98.94(n) 98.41(a)	473195 XM_528565.2 XP_528565.2

About this table Species with no ortholog for IFNA5

ENSEMBL Gene Tree for IFNA5

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for IFNA5 gene

IFNA1² IFNA21² IFNA2² IFNA7² IFNA17² IFNA4² IFNA6² IFNA14²

IFNA13² IFNA16² IFNA10² IFNA8²

Pseudogenes

5 related pseudogenes

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/15 NCBI SNPs in IFNA5 are shown (see all 15 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 25)

AB	SNP ID	Valid	Chr 9 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs7031048^1,2	A,C,F,H	21296318(+)	`cttccA/Gtgaac`	1	--	ng3¹		4		EU EA WA	418
	rs12156640^1,2	C,F,H	21296241(+)	`atcttG/Agaaag`	1	--	ng3¹ tfbs³		4		EU EA WA	418
--	rs1952691^1,2	C,F,H	21296019(+)	`aaaaaA/Cctttt`	1	--	ng3¹ trp³		4		EU EA WA	418
--	rs7027327^1,2	A,C	21295855(+)	`atataA/Gtattg`	1	--	ng3¹		0	--	--	--	--
	rs28383775^1,2	C	21294245(-)	`CCTGAC/TTGTAT`	1	--	ng5¹		0	--	--	--	--
	rs10757212^1,2	A,C,F,H	21294803(+)	`TAGAGG/AGTGAT`	1	T/T	syn¹		7		NA EU EA WA	558
--	rs35886555^1,2	--	21295260(+)	`TGGGG-/GAGGTT`	1	--	ng3¹		0	--	--	--	--
--	rs41298198^1,2	--	21294913(+)	`CAGCTA/GCTGGT`	1	Q/X	non¹		0	--	--	--	--
--	rs72695780^1,2	--	21297072(+)	`ATGGAA/GACAAA`	1	--	ng3¹		0	--	--	--	--
--	rs34679967^1,2	--	21294200(+)	`TAATG-/CATAGG`	1	--	ng5¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for IFNA5 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 147565

Human Genome Epidemiology Navigator: IFNA5 (4 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/17 PubMed articles for IFNA5 gene (see all 17 ):

DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)^{3, 4} Humphray S.J.... Dunham I. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Signal peptide prediction based on analysis of experimentally verified cleavage sites. (PubMed id 15340161)^{3, 4} Zhang Z. and Henzel W.J. (2004)
Mapping of the shortest region of overlap of deletions of the short arm of chromosome 9 associated with human neoplasia. (PubMed id 1385305)^{2, 3} Olopade O.I....Diaz M.O. (1992)
Structural relationship of human interferon alpha genes and pseudogenes. (PubMed id 4057246)^{3, 4} Henco K.... Weissmann C. (1985)
The structure of eight distinct cloned human leukocyte interferon cDNAs. (PubMed id 6163083)^{3, 4} Goeddel D.V.... Gray P.W. (1981)
Genetic susceptibility to respiratory syncytial virus bronchiolitis in preterm children is associated with airway remodeling genes and innate immune genes. (PubMed id 19258923)³ Siezen C.L....Janssen R. (2009)
Polymorphisms of interferons and their receptors in the genetics of severe RSV-associated diseases. (PubMed id 18953482)³ Mailaparambil B....Heinzmann A. (2008)
Genetic association of interferon-alpha subtypes 1, 2 and 5 in systemic lupus erythematosus. (PubMed id 19000144)³ Hirankarn N....Avihingsanon Y. (2008)
Genetic susceptibility to respiratory syncytial virus bronchiolitis is predominantly associated with innate immune genes. (PubMed id 17703412)³ Janssen R....Hoebee B. (2007)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3442	HGNC: 5426	AceView: IFNA5	Ensembl:ENSG00000147873	euGenes: HUgn3442
ECgene: IFNA5	H-InvDB: IFNA5

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for IFNA5
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for IFNA5:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for IFNA5-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for IFNA5		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for IFNA5		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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Antibodies for IFNA5

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IFNA5 Gene

protein-coding GIFtS: 59

Aliases &
Descriptions
for IFNA5

Summaries
for IFNA5

Genomic Location
for IFNA5

Proteins
for IFNA5

Protein Domains/
Families
for IFNA5

Gene Function
for IFNA5

Pathways & Interactions
for IFNA5

Drugs & Compounds
for IFNA5

Transcripts
for IFNA5

Expression
for IFNA5

Orthologs
for IFNA5

Paralogs
for IFNA5

SNPs/Variants
for IFNA5

Disorders & Mutations
for IFNA5

Medical News
for IFNA5

Publications
for IFNA5

Search for IFNA5

Genome Databases showing IFNA5

Other Databases showing IFNA5

Specialized Databases showing IFNA5

Licensable Technologies
for IFNA5

Services
for IFNA5

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

IFNA5 Gene

protein-coding GIFtS: 59

Aliases & Descriptionsfor IFNA5

Summariesfor IFNA5

Genomic Locationfor IFNA5

Proteinsfor IFNA5

Protein Domains/Familiesfor IFNA5

Gene Functionfor IFNA5

Pathways & Interactionsfor IFNA5

Drugs & Compoundsfor IFNA5

Transcriptsfor IFNA5

Expression for IFNA5

Orthologsfor IFNA5

Paralogsfor IFNA5

SNPs/Variantsfor IFNA5

Disorders & Mutationsfor IFNA5

Medical Newsfor IFNA5

Publicationsfor IFNA5

Search for IFNA5

Genome Databases showing IFNA5

Other Databases showing IFNA5

Specialized Databases showing IFNA5

Licensable Technologiesfor IFNA5

Servicesfor IFNA5

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for IFNA5

Summaries
for IFNA5

Genomic Location
for IFNA5

Proteins
for IFNA5

Protein Domains/
Families
for IFNA5

Gene Function
for IFNA5

Pathways & Interactions
for IFNA5

Drugs & Compounds
for IFNA5

Transcripts
for IFNA5

Expression
for IFNA5

Orthologs
for IFNA5

Paralogs
for IFNA5

SNPs/Variants
for IFNA5

Disorders & Mutations
for IFNA5

Medical News
for IFNA5

Publications
for IFNA5

Licensable Technologies
for IFNA5

Services
for IFNA5