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IFNA4 Gene

protein-coding GIFtS: 54

GC09M021176

interferon, alpha 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
INFA4 ^²
MGC142200 ^¹, ²
OTTHUMP00000021135 ^²
OTTHUMP00000045102 ^²

Descriptions
Interferon alpha-4B ^³
Interferon alpha-76 ^³
Interferon alpha-M1 ^³
interferon, alpha 4 ^²

External Ids
HGNC: 5425¹
Entrez Gene: 3441²
UniProtKB: P05014³
Ensembl: ENSG00000147877⁷

Search outside databases for aliases for IFNA4 gene

Previous GC identifers: GC09M021514 GC09M021387

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: IFNA4_HUMAN, P05014

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Gene Wiki entry for IFNA4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the IFNA4 gene

Entrez Gene cytogenetic band: 9p22 Ensembl cytogenetic band: 9p21.3 HGNC cytogenetic band: 9p22

IFNA4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09M021176: (about GC identifiers)

Start:	21,176,693 bp from pter
End:	21,177,670 bp from pter
Size:	978 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000009.10 NT_008413.17

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: IFNA4_HUMAN, P05014 (See protein sequence)

Recommended Name: Interferon alpha-4 precursor

Size: 189 amino acids; 21808 Da

Subcellular location: Secreted

Secondary accessions: P13358 Q5VV15

REFSEQ proteins: NP_066546.1

ENSEMBL proteins:

ENSP00000369571

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	R&D Systems Recombinant & Natural Proteins for IFNA4 (IFN-alpha 4)
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (IFNA4)
	Human Recombinant Proteins from Abnova (IFNA4)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--
GO:0005615	extracellular space	IEA	--

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Antibodies for IFNA4:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for IFNA4
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (IFNA4), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (IFNA4)
	Novus Biologicals Antibody for IFNA4

Assays for IFNA4:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry P05014

ProtoNet protein and cluster: P05014

1 Blocks protein family: IPB000471 Interferon alpha

UniProtKB/Swiss-Prot: IFNA4_HUMAN, P05014

Similarity: Belongs to the alpha/beta interferon family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021068

Applied Biosystems Silencer® siRNAs for IFNA4

Browse siRNA at Sigma-Aldrich

Sigma-Aldrich shRNA Panels and shRNA for IFNA4

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for IFNA4
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_021068
Myc/DDK tagged cDNA clone in CMV expression vector: NM_021068
untagged cDNA clone in CMV expression vector: NM_021068

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_021068

UniProtKB/Swiss-Prot: IFNA4_HUMAN, P05014

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Genatlas biochemistry entry for IFNA4:

interferon type I,alpha 4,leukocyte

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005125	cytokine activity	IEA	--
GO:0005132	interferon-alpha/beta receptor binding	TAS	3891272

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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4 Sigma-Aldrich "Your Favorite Gene" Pathways for IFNA4 (Your Favorite Gene powered by Ingenuity)

Role of RIG1-like Receptors in Antiviral Innate Immunity
Role of Cytokines in Mediating Communication between Immune Cells
IL-12 Signaling and Production in Macrophages
Activation of IRF by Cytosolic Pattern Recognition Receptors

GeneDecks IFNA4 for the pathways selected above

About GeneDecksing

2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006952	defense response	IEA	--
GO:0009615	response to virus	TAS	3891272

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for IFNA4

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_021068

Browse siRNA at Sigma-Aldrich

Sigma-Aldrich shRNA Panels and shRNA for IFNA4

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_021068

REFSEQ mRNAs for IFNA4 gene:

NM_021068.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_021068

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_021068
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_021068
                                 untagged cDNA clone in CMV expression vector: NM_021068

Additional cDNA sequence:

BC074965.2 BC074966.2 BC113640.1 BC113642.1 M27318.1

1 DOTS entry:

DT.121148358

11 AceView cDNA sequences:

BC074966 BV199635 BV199629 M27318 NM_021068 BV199634 BC074965 BV199637

BV199636 BV199639 BV199642

highest scoring ESTs for IFNA4:

BC074965 BC074966 M27318 BC113640 BC113642 NM_021068

Unigene Cluster for IFNA4:

Interferon, alpha 4

Hs.1510

Unigene Representative Sequence: NM_021068

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000380222

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

IFNA4 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for IFNA4

¹ / ² / ³

5 probe-sets matching IFNA4 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
1580_f_at²^{, 3}	U95-A	15	1.00	0.24	1.00	1.00	M27318	0.80	0.80	0.80	1
207964_x_at²^{, 3}	U133-A	13	1.00	0.31	--	--	NM_021068	0.40	0.67	0.55	1
211405_x_at²	U133-A	13	0.82	0.13	--	--	--	--	--	--	--
207964_x_at²	U133Plus2	13	1.00	0.31	--	--	--	--	--	--	--
211405_x_at²	U133Plus2	13	0.82	0.13	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.1510
Expression variation in blood from EXPOLDB for IFNA4

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for IFNA4 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	IFNA4¹	--	interferon, alpha 4	96.65(n) 94.71(a)	741890 XM_001151949.1 XP_001151949.1

About this table Species with no ortholog for IFNA4

ENSEMBL Gene Tree for IFNA4

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for IFNA4 gene

IFNA1² IFNA21² IFNA2² IFNA7² IFNA17² IFNA6² IFNA14² IFNA13²

IFNA16² IFNA10² IFNA8² IFNA5²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

UniProtKB/Swiss-Prot: IFNA4_HUMAN, P05014

Polymorphism: Two forms exist; alpha-4a (shown here) and alpha-4b. They seem to be equally abundant

10/29 NCBI SNPs in IFNA4 are shown (see all 29 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 11)

AB	SNP ID	Valid	Chr 9 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
--	rs7870840^1,2	A,C,F,H	21177928(+)	`aaagtT/Caaact`	1	--	ng3¹		4		EU EA WA	406
--	rs7035639^1,2	A,C,F,H	21179262(+)	`ATGTAG/CATCTG`	1	--	ng3¹		7		EU EA WA NA	558
--	rs3750479^1,2	A,C,F,H	21176931(+)	`AGTCAA/GTCAGG`	1	--	ut3¹		12		EA EU WA NA	2438
--	rs1062571^1,2	A,C,F	21177311(-)	`AGAAGA/GCTCAA`	1	A/T	mis¹ ese³		1		EA	1448
--	rs1062574^1,2	F,H	21177115(-)	`GGACTC/TCATCC`	1	S/F	mis¹ ese³		5		MN EU EA WA	600
--	rs10964896^1,2	F,H	21178207(+)	`CTTCAC/TCAGCT`	1	--	ng3¹		4		EU EA WA	418
--	rs10964899^1,2	A	21178352(+)	`ACATCC/TTGAAG`	1	--	ng3¹		0	--	--	--	--
--	rs3955235^1,2	A	21177773(+)	`CAAAGC/GCTTTT`	1	--	ng3¹		0	--	--	--	--
--	rs12236048^1,2	A	21176254(+)	`TATCTC/GTGCTT`	1	--	ng5¹		0	--	--	--	--
--	rs10811494^1,2	A	21179377(+)	`AATAAC/GTAGCC`	1	--	ng3¹		0	--	--	--	--

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HapMap Linkage Disequilibrium images for IFNA4 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 147564

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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10/18 PubMed articles for IFNA4 gene (see all 18 ):

DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)^{3, 4} Humphray S.J.... Dunham I. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes. (PubMed id 9425112)^{3, 4} Nyman T.A.... Kalkkinen N. (1998)
Both variant forms of interferon-alpha4 gene (IFNA4a and IFNA4b) are present in the human population. (PubMed id 9335434)^{3, 4} Hussain M.... Liao M.-J. (1997)
Mapping of the shortest region of overlap of deletions of the short arm of chromosome 9 associated with human neoplasia. (PubMed id 1385305)^{2, 3} Olopade O.I....Diaz M.O. (1992)
Structural relationship of human interferon alpha genes and pseudogenes. (PubMed id 4057246)^{3, 4} Henco K.... Weissmann C. (1985)
Nucleotide sequence and expression in E. coli of a human interferon- alpha gene selected from a genomic library using synthetic oligonucleotides. (PubMed id 6089830)^{3, 4} Linnane A.W.... Woodrow G.C. (1984)
Induction of suppressor of cytokine signaling-3 by herpes simplex virus type 1 contributes to inhibition of the interferon signaling pathway. (PubMed id 15163721)³ Yokota S....Fujii N. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Alpha interferon suppresses the cyclin D3 and cdc25A genes, leading to a reversible G0-like arrest. (PubMed id 8668211)³ Tiefenbrun N....Kimchi A. (1996)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3441	HGNC: 5425	AceView: IFNA4	Ensembl:ENSG00000147877	euGenes: HUgn3441
ECgene: IFNA4	H-InvDB: IFNA4

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for IFNA4
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for IFNA4:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for IFNA4-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Browse siRNAs at Sigma-Aldrich		Antibodies for IFNA4
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for IFNA4		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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Antibodies for IFNA4

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IFNA4 Gene

protein-coding GIFtS: 54

Aliases &
Descriptions
for IFNA4

Summaries
for IFNA4

Genomic Location
for IFNA4

Proteins
for IFNA4

Protein Domains/
Families
for IFNA4

Gene Function
for IFNA4

Pathways & Interactions
for IFNA4

Drugs & Compounds
for IFNA4

Transcripts
for IFNA4

Expression
for IFNA4

Orthologs
for IFNA4

Paralogs
for IFNA4

SNPs/Variants
for IFNA4

Disorders & Mutations
for IFNA4

Medical News
for IFNA4

Publications
for IFNA4

Search for IFNA4

Genome Databases showing IFNA4

Other Databases showing IFNA4

Specialized Databases showing IFNA4

Licensable Technologies
for IFNA4

Services
for IFNA4

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

IFNA4 Gene

protein-coding GIFtS: 54

Aliases & Descriptionsfor IFNA4

Summariesfor IFNA4

Genomic Locationfor IFNA4

Proteinsfor IFNA4

Protein Domains/Familiesfor IFNA4

Gene Functionfor IFNA4

Pathways & Interactionsfor IFNA4

Drugs & Compoundsfor IFNA4

Transcriptsfor IFNA4

Expression for IFNA4

Orthologsfor IFNA4

Paralogsfor IFNA4

SNPs/Variantsfor IFNA4

Disorders & Mutationsfor IFNA4

Medical Newsfor IFNA4

Publicationsfor IFNA4

Search for IFNA4

Genome Databases showing IFNA4

Other Databases showing IFNA4

Specialized Databases showing IFNA4

Licensable Technologiesfor IFNA4

Servicesfor IFNA4

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for IFNA4

Summaries
for IFNA4

Genomic Location
for IFNA4

Proteins
for IFNA4

Protein Domains/
Families
for IFNA4

Gene Function
for IFNA4

Pathways & Interactions
for IFNA4

Drugs & Compounds
for IFNA4

Transcripts
for IFNA4

Expression
for IFNA4

Orthologs
for IFNA4

Paralogs
for IFNA4

SNPs/Variants
for IFNA4

Disorders & Mutations
for IFNA4

Medical News
for IFNA4

Publications
for IFNA4

Licensable Technologies
for IFNA4

Services
for IFNA4