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IFNA21 Gene

protein-coding GIFtS: 57

GC09M021155

interferon, alpha 21
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
MGC126687 ^²
MGC126689 ^²
OTTHUMP00000021132 ^²

Descriptions
Interferon alpha-F ^³
LeIF F ^³
interferon, alpha 21 ^²
leukocyte interferon protein ^²

External Ids
HGNC: 5424¹
Entrez Gene: 3452²
UniProtKB: P01568³
Ensembl: ENSG00000137080⁷

Search outside databases for aliases for IFNA21 gene

Previous GC identifers: GC09M021493 GC09M021366

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: IFN21_HUMAN, P01568

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Gene Wiki entry for IFNA21

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the IFNA21 gene

Entrez Gene cytogenetic band: 9p22 Ensembl cytogenetic band: 9p21.3 HGNC cytogenetic band: 9p22

IFNA21 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09M021155: (about GC identifiers)

Start:	21,155,636 bp from pter
End:	21,156,659 bp from pter
Size:	1,024 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000009.10 NT_008413.17

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: IFN21_HUMAN, P01568 (See protein sequence)

Recommended Name: Interferon alpha-21 precursor

Size: 189 amino acids; 21741 Da

Subcellular location: Secreted

Secondary accessions: Q14608 Q5VWD1 Q7M4Q4

REFSEQ proteins: NP_002166.2

ENSEMBL proteins:

ENSP00000369574

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	R&D Systems Recombinant & Natural Proteins for IFNA21 (IFN-alpha F)
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (IFNA21)
	Human Recombinant Proteins from Abnova (IFNA21)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--
GO:0005615	extracellular space	IEA	--

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Antibodies for IFNA21:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (IFNA21), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (IFNA21)
	Novus Biologicals Antibodies for IFNA21

Assays for IFNA21:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry P01568

ProtoNet protein and cluster: P01568

1 Blocks protein family: IPB000471 Interferon alpha

UniProtKB/Swiss-Prot: IFN21_HUMAN, P01568

Similarity: Belongs to the alpha/beta interferon family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002175

Applied Biosystems Silencer® siRNAs for IFNA21

Sigma-Aldrich siRNA and siRNA Panels for IFNA21

Sigma-Aldrich shRNA Panels and shRNA for IFNA21

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for IFNA21
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_002175
Myc/DDK tagged cDNA clone in CMV expression vector: NM_002175
untagged cDNA clone in CMV expression vector: NM_002175

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_002175

UniProtKB/Swiss-Prot: IFN21_HUMAN, P01568

Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the

production of two enzymes: a protein kinase and an oligoadenylate synthetase

Genatlas biochemistry entry for IFNA21:

interferon type I,alpha 21,leukocyte

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005125	cytokine activity	IEA	--
GO:0005126	hematopoietin/interferon-class (D200-domain) cytokine receptor binding	TAS	6163083

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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4 Sigma-Aldrich "Your Favorite Gene" Pathways for IFNA21 (Your Favorite Gene powered by Ingenuity)

Role of RIG1-like Receptors in Antiviral Innate Immunity
Role of Cytokines in Mediating Communication between Immune Cells
Activation of IRF by Cytosolic Pattern Recognition Receptors
IL-12 Signaling and Production in Macrophages

GeneDecks IFNA21 for the pathways selected above

About GeneDecksing

5/19 Interacting proteins for IFNA21 (ENSP00000369574³) via UniProtKB, MINT, and/or STRING (see all 19 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
IFNG	ENSP00000229135³	STRING (score=.915)
IFNA14	ENSP00000369569³	STRING (score=.9)
IFNA17	ENSP00000369562³	STRING (score=.9)
IFNA5	ENSP00000369560³	STRING (score=.9)
IFNA8	ENSP00000369553³	STRING (score=.9)

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2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006952	defense response	IEA	--
GO:0009615	response to virus	IEA	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for IFNA21

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_002175

Sigma-Aldrich siRNA and siRNA Panels for IFNA21

Sigma-Aldrich shRNA Panels and shRNA for IFNA21

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_002175

REFSEQ mRNAs for IFNA21 gene:

NM_002175.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_002175

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_002175
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_002175
                                 untagged cDNA clone in CMV expression vector: NM_002175

Additional cDNA sequence:

BC069329.1 BC069372.1 BC069408.1 BC096699.1 BC101638.1 BC101640.1 M12350.1 M28586.1

V00540.1 X00145.1

1 DOTS entry:

DT.108640

11 AceView cDNA sequences:

BC069408 BC069329 BC069372 X00145 M28586 M12350 BV199628 BV199632

NM_002175 V00540 BV199630

highest scoring ESTs for IFNA21:

V00540 BC069329 BC069372 BC069408 M12350 M28586 X00145 BC096699 NM_002175 BC101638

Unigene Cluster for IFNA21:

Interferon, alpha 21

Hs.113211

Unigene Representative Sequence: NM_002175

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000380225

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

IFNA21 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for IFNA21

¹ / ² / ³

3 probe-sets matching IFNA21 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
1435_f_at²^{, 3}	U95-A	14	1.00	0.69	1.00	1.00	V00540	1.00	1.00	1.00	1
211145_x_at²^{, 3}	U133-A	13	1.00	0.42	--	--	M12350	0.80	1.00	0.91	1
211145_x_at²	U133Plus2	13	1.00	0.42	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: TAATATTATG

SOURCE GeneReport for Unigene cluster: Hs.113211

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for IFNA21 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	LOC473185¹	--	hypothetical LOC473185	99.29(n) 99.47(a)	473185 XM_528555.2 XP_528555.1

About this table Species with no ortholog for IFNA21

ENSEMBL Gene Tree for IFNA21

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for IFNA21 gene

IFNA1² IFNA2² IFNA7² IFNA17² IFNA6² IFNA4² IFNA14² IFNA13²

IFNA16² IFNA10² IFNA8² IFNA5²

Pseudogenes

1 related pseudogene

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/43 NCBI SNPs in IFNA21 are shown (see all 43 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 25)

AB	SNP ID	Valid	Chr 9 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
--	rs2891157^1,2	A,C,F,H,O	21158306(+)	`AACCAC/GTGGGA`	1	--	ng3¹		7		EU EA WA NA	560
--	rs7021036^1,2	A,F,H	21158482(+)	`TCACAC/TGGTTT`	1	--	ng3¹		4		EU EA WA	416
--	rs3750478^1,2	C,F,H	21156077(+)	`AATTTT/CTGATA`	1	E/K	mis¹		12		EA NA EU WA	2458
--	rs10811487^1,2	A,F,H	21158139(+)	`TAAGTG/AGAGTT`	1	--	ng3¹		4		EU EA WA	408
--	rs7021065^1,2	A,C,F	21158593(+)	`TGTGTA/GTGTGA`	1	--	ng3¹		3		NA	142
--	rs7047687^1,2	C,F,H	21157652(+)	`aatacA/Cgtgct`	1	--	ng3¹		4		EU EA WA	420
--	rs7047299^1,2	A,C	21157465(+)	`acataA/Ggcaat`	1	--	ng3¹ tfbs³		0	--	--	--	--
--	rs12376071^1,2	A,F	21156902(+)	`ttagtA/Gacaca`	1	--	ng3¹		1		NS	1182
	rs7038852^1,2	A,C	21155322(+)	`TGTCTA/GCTATA`	1	--	ng5¹		0	--	--	--	--
	rs7024135^1,2	A,C	21155348(+)	`TTTTTA/TAAAAA`	1	--	ng5¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for IFNA21 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 147584

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/13 PubMed articles for IFNA21 gene (see all 13 ):

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes. (PubMed id 9425112)^{3, 4} Nyman T.A.... Kalkkinen N. (1998)
Identification of interferon-alpha 7, -alpha 14, and -alpha 21 variants in the genome of a large human population. (PubMed id 8910771)^{3, 4} Hussain M.... Liao M.-J. (1996)
Mapping of the shortest region of overlap of deletions of the short arm of chromosome 9 associated with human neoplasia. (PubMed id 1385305)^{2, 3} Olopade O.I....Diaz M.O. (1992)
Novel human leukocyte interferon subtype and structural comparison of alpha interferon genes. (PubMed id 6548765)^{3, 4} Gren E....Apsalons U. (1984)
The structure of eight distinct cloned human leukocyte interferon cDNAs. (PubMed id 6163083)^{3, 4} Goeddel D.V.... Gray P.W. (1981)
DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)⁴ Humphray S.J.... Dunham I. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
[Expression of interferon-alpha mRNA in human brain tissues] (PubMed id 9027905)³ Kawaguchi N....Yoshiyama Y. (1997)
Alpha interferon suppresses the cyclin D3 and cdc25A genes, leading to a reversible G0-like arrest. (PubMed id 8668211)³ Tiefenbrun N....Kimchi A. (1996)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3452	HGNC: 5424	AceView: IFNA21	Ensembl:ENSG00000137080	euGenes: HUgn3452
ECgene: IFNA21	H-InvDB: IFNA21

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for IFNA21
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for IFNA21:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for IFNA21-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for IFNA21		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for IFNA21		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (IFNA21)
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	Custom Service for Rabbit Pab from Full-length Protein

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Search Tocris compounds for IFNA21

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for IFNA21

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IFNA21 Gene at Home site.
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IFNA21 Gene

protein-coding GIFtS: 57

Aliases &
Descriptions
for IFNA21

Summaries
for IFNA21

Genomic Location
for IFNA21

Proteins
for IFNA21

Protein Domains/
Families
for IFNA21

Gene Function
for IFNA21

Pathways & Interactions
for IFNA21

Drugs & Compounds
for IFNA21

Transcripts
for IFNA21

Expression
for IFNA21

Orthologs
for IFNA21

Paralogs
for IFNA21

SNPs/Variants
for IFNA21

Disorders & Mutations
for IFNA21

Medical News
for IFNA21

Publications
for IFNA21

Search for IFNA21

Genome Databases showing IFNA21

Other Databases showing IFNA21

Specialized Databases showing IFNA21

Licensable Technologies
for IFNA21

Services
for IFNA21

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

IFNA21 Gene

protein-coding GIFtS: 57

Aliases & Descriptionsfor IFNA21

Summariesfor IFNA21

Genomic Locationfor IFNA21

Proteinsfor IFNA21

Protein Domains/Familiesfor IFNA21

Gene Functionfor IFNA21

Pathways & Interactionsfor IFNA21

Drugs & Compoundsfor IFNA21

Transcriptsfor IFNA21

Expression for IFNA21

Orthologsfor IFNA21

Paralogsfor IFNA21

SNPs/Variantsfor IFNA21

Disorders & Mutationsfor IFNA21

Medical Newsfor IFNA21

Publicationsfor IFNA21

Search for IFNA21

Genome Databases showing IFNA21

Other Databases showing IFNA21

Specialized Databases showing IFNA21

Licensable Technologiesfor IFNA21

Servicesfor IFNA21

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for IFNA21

Summaries
for IFNA21

Genomic Location
for IFNA21

Proteins
for IFNA21

Protein Domains/
Families
for IFNA21

Gene Function
for IFNA21

Pathways & Interactions
for IFNA21

Drugs & Compounds
for IFNA21

Transcripts
for IFNA21

Expression
for IFNA21

Orthologs
for IFNA21

Paralogs
for IFNA21

SNPs/Variants
for IFNA21

Disorders & Mutations
for IFNA21

Medical News
for IFNA21

Publications
for IFNA21

Licensable Technologies
for IFNA21

Services
for IFNA21