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IFI35 Gene

protein-coding   GIFtS: 53

GC17P038412
interferon-induced protein 35
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ21753 2
IFP35 1, 2, 3
Descriptions
IFP 35 3
interferon-induced protein 35 2
External Ids
HGNC: 53991
Entrez Gene: 34302
UniProtKB: P802173
Ensembl: ENSG000000680797
Search outside databases for aliases for IFI35 gene

Previous GC identifers: GC17M062367 GC17P043320 GC17P041067 GC17P041532 GC17P041533

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: IN35_HUMAN, P80217
Function: Not yet known

Gene Wiki entry for IFI35

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the IFI35 gene  

Entrez Gene cytogenetic band: 17q21   Ensembl cytogenetic band:  17q21.31   HGNC cytogenetic band: 17q21

IFI35 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P038412:     (about GC identifiers)

Start:
38,412,351 bp from pter
End:
38,419,999 bp from pter
Size:
7,649 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_010755.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: IN35_HUMAN, P80217 (See protein sequence)
Recommended Name: Interferon-induced 35 kDa protein  
Size: 286 amino acids; 31514 Da
Subunit: Homodimer. Also interacts with B-ATF
Subcellular location: Nucleus. Note=Nuclear following IFN treatment
Secondary accessions: Q92984 Q99537 Q9BV98
Alternative splicing: 2 isoforms:  P80217-1   P80217-2   

REFSEQ proteins: NP_005524.1  

ENSEMBL proteins: 
ENSP00000246911 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (IFI35)
Human Recombinant Proteins from Abnova (IFI35)
              OriGene Purified Recombinant Human Protein: IFI35 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IDA8288566
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Antibodies for IFI35: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for IFI35
Browse R&D Systems for Antibodies
Antibodies from Abcam (IFI35), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (IFI35)
Novus Biologicals Antibodies for IFI35

Assays for IFI35: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR009938 Interferon_induced_35kDa_N
 IPR009909 Nmi/IFP35


   GeneDecks  IFI35 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P80217

ProtoNet protein and cluster: P80217

1 Blocks protein family: IPB009909 Nmi/IFP 35

UniProtKB/Swiss-Prot: IN35_HUMAN, P80217
Similarity: Belongs to the NMI family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (IFI35)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (IFI35)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005533

              Applied Biosystems Silencer® siRNAs for IFI35

              Sigma-Aldrich siRNA for IFI35  
                     Sigma-Aldrich shRNA for IFI35  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for IFI35
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005533
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005533
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_005533 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_005533

UniProtKB/Swiss-Prot: IN35_HUMAN, P80217
Function: Not yet known
Induction: By interferon gamma

Genatlas biochemistry entry for IFI35:
interferon-induced leucine zipper protein IFI35,may be the same as APRF

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI8954125
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(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  IFI35  (Your Favorite Gene powered by Ingenuity) 
 Interferon Signaling

   GeneDecks  IFI35 for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  IFI35 


4 Interacting proteins for IFI35 (P802171 ENSP000002469113) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
CLEC4GQ6UXB41EBI-2115067, EBI-2114729
PLEKHO1ENSP000000254693STRING (score=.948)
BATFENSP000002866393STRING (score=.852)
MYCNENSP000002810433STRING (score=.771)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006955 immune response NAS--
GO:0009615 response to virus NAS--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for IFI35
1 Novoseek chemical compound relationship for IFI35 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
leucine 59.06 7 8288566 (3), 8938427 (1), 8668343 (1), 8954125 (1)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (IFI35)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (IFI35)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005533

              Sigma-Aldrich siRNA for IFI35  
                     Sigma-Aldrich shRNA for IFI35  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_005533  

REFSEQ mRNAs for IFI35 gene: 

NM_005533.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_005533  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005533
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005533
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_005533 

Additional cDNA sequence: 

AK025406.1 BC001356.2 U72882.1 

7 DOTS entries:

DT.455598  DT.100028280  DT.91753253  DT.92389823  DT.75126564  DT.100778859  DT.120983472 

24/201 AceView cDNA sequences (see all 201 ):

BM996594 BU629908 BE276568 CB130670 CB110077 AW082544 AW118002 BX954767 
BQ020479 CB138771 CA748732 BM717524 BM694278 BG749376 BG168316 BQ921917 
BE383727 BM857058 BU732151 BM556210 BM806352 BG476389 AA195653 BE735639 

highest scoring ESTs for IFI35:

AW026462 U72882 AA025690 AA026789 AA088879 AA115662 AA132931 AA195653 AA435595 AA536149 

Unigene Cluster for IFI35:

Interferon-induced protein 35
Hs.632258  [show with all ESTs]
Unigene Representative Sequence: BU539084


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000246911  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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IFI35 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for IFI35

1 / 2 / 3

5 probe-sets matching IFI35 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

626_s_at2, 3 U95-A 1 1.00 1.00 0.95 0.94 L78833 0.20 0.25 0.23 1
464_s_at2, 3 U95-A 1 1.00 1.00 0.97 1.11 U72882 1.00 1.00 1.00 1
74908_at2, 3 U95-D 1 0.81 1.00 0.99 0.97 AW026462 1.00 1.00 1.00 1

209417_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC001356 0.80 1.00 0.91 1

209417_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  IFI35 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ACGTCCTGGA

SOURCE GeneReport for Unigene cluster: Hs.632258

UniProtKB/Swiss-Prot: IN35_HUMAN, P80217
Tissue specificity: In a wide range of cell types, including fibroblasts, macrophages, and
epithelial cells

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for IFI35 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
IFI351   -- interferon-induced protein 35 79.12(n)
74.73(a)
490954  XM_548077.2  XP_548077.2 
chimpanzee
(Pan troglodytes)
IFI351   -- interferon-induced protein 35 99.07(n)
98.6(a)
741105  XM_001158983.1  XP_001158983.1 
cow
(Bos taurus)
IFI351   -- interferon-induced protein 35 80.84(n)
74.71(a)
510697  NM_001075462.1  NP_001068930.1 
rat
(Rattus norvegicus)
Ifi351   -- interferon-induced protein 35 79.06(n)
72.98(a)
287719  NM_001009625.1  NP_001009625.1 
mouse
(Mus musculus)
Ifi351, 5 115
interferon-induced protein 351, 5 78.48(n)1
72.28(a)1
701101  NM_027320.31  NP_081596.11 
 AI3259205  AK0081615  (see all 11)
About this table        Species with no ortholog for IFI35

ENSEMBL Gene Tree for IFI35
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for IFI35 gene
NMI2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/70 NCBI SNPs in IFI35 are shown (see all 70 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 40)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 17 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs7759901,2
C,F,H38412059(-) GAACAT/CTGATA 1 -- ng518Minor allele frequency- C:0.01EU EA WA 834
rs5887031,2
C,F,H38419019(-) GGACAC/TCTAGG 1 V/M mis19Minor allele frequency- T:0.02MN EU EA WA 1022
rs11082871,2
C,F38410631(-) AGGAGC/AGCTAT 1 -- ng513Minor allele frequency- A:0.32NA 140
--
rs7759931,2
C38411800(-) gagatC/Ggtgcc 1 -- ng510--------
--
rs129411731,2
A38411138(+) tagtcA/Tgaatc 1 -- ng510--------
--
rs7759911,2
A38411908(-) aaataC/Taaaaa 1 -- ng510--------
--
rs6921191,2
C38410913(-) gaggcA/Ggtgaa 1 -- ng510--------
--
rs7759921,2
C38411806(-) tgagcC/Tgagat 1 -- ng510--------
rs132291,2
C,F,H38419404(+) GTGAAT/CGGGGA 1 N/N syn15Minor allele frequency- C:0.01MN EU EA WA 592
rs6911441,2
A,C,F,H38417175(-) agcccG/Atctga 1 -- int13Minor allele frequency- A:0.16EU EA 298
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HapMap Linkage Disequilibrium images for IFI35 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
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OMIM: 600735

1 Novoseek disease relationship for IFI35 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tumors 0.00 1 17197158 (1)
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(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/15 PubMed articles for IFI35 gene (see all 15 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3430 HGNC: 5399 AceView: IFI35 Ensembl:ENSG00000068079 euGenes: HUgn3430
ECgene: IFI35 H-InvDB: IFI35
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for IFI35:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for IFI35-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for IFI35 Antibodies for IFI35
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for IFI35 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(IFI35)
 Antibodies (IFI35)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for IFI35




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for IFI35

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