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IFI30 Gene

protein-coding   GIFtS: 56

GC19P018145
interferon, gamma-inducible protein 30
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
GILT 1, 2, 3
IFI-30 1, 2
IP30 1, 2
MGC32056 1, 2
Descriptions
Gamma-interferon-inducible protein IP-30 3
gamma-interferon-inducible lysosomal thiol reductase 2
interferon gamma-inducible protein 30 preproprotein 2
interferon, gamma-inducible protein 30 2
External Ids
HGNC: 53981
Entrez Gene: 104372
UniProtKB: P132843
Ensembl: ENSG000002164907
Search outside databases for aliases for IFI30 gene

Previous GC identifers: GC19P018778 GC19P018676 GC19P018129

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for IFI30:
The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein
disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by
gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted
antigen processing. [provided by RefSeq]

UniProtKB/Swiss-Prot: GILT_HUMAN, P13284
Function: Cleaves disulfide bonds in proteins by reduction. May facilitate the complete unfolding
of proteins destined for lysosomal degradation. May be involved in MHC class II-restricted antigen
processing

Gene Wiki entry for IFI30

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the IFI30 gene  

Entrez Gene cytogenetic band: 19p13.1   Ensembl cytogenetic band:  19p13.11   HGNC cytogenetic band: 19p13.1

IFI30 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P018145:     (about GC identifiers)

Start:
18,145,579 bp from pter
End:
18,149,927 bp from pter
Size:
4,349 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011295.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: GILT_HUMAN, P13284 (See protein sequence)
Recommended Name: Gamma-interferon-inducible lysosomal thiol reductase precursor  
Size: 261 amino acids; 29149 Da
Subcellular location: Lysosome
Secondary accessions: Q9UL08

Post-translational modifications:

  • N-glycosylated. Sugar chains contain mannose-6-phosphate1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006323.2  

    ENSEMBL proteins: 
    ENSP00000384886 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam ( IFI30 )
    Human Recombinant Proteins from Abnova (IFI30)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region TAS3136170
    GO:0005764 lysosome TAS3136170
    About this table

    Antibodies for IFI30: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for IFI30
    Browse R&D Systems for Antibodies
    Antibodies from Abcam ( IFI30 ), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (IFI30)
    Novus Biologicals Antibodies for IFI30

    Assays for IFI30: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR004911 GILT


       GeneDecks  IFI30 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P13284

    ProtoNet protein and cluster: P13284

    UniProtKB/Swiss-Prot: GILT_HUMAN, P13284
    Similarity: Belongs to the GILT family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (IFI30)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006332

                  Applied Biosystems Silencer® siRNAs for IFI30

                  Sigma-Aldrich siRNA for IFI30  
                         Sigma-Aldrich shRNA for IFI30  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for IFI30
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006332
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006332
                                     untagged cDNA clone in CMV expression vector: NM_006332 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006332

    UniProtKB/Swiss-Prot: GILT_HUMAN, P13284
    Function: Cleaves disulfide bonds in proteins by reduction. May facilitate the complete unfolding
    of proteins destined for lysosomal degradation. May be involved in MHC class II-restricted antigen
    processing
    Induction: Expressed constitutively in antigen-presenting cells and induced by IFN-gamma in other
    cell types

    Genatlas biochemistry entry for IFI30:
    interferon, gamma-inducible protein 30,present and inducible by gamma-interferon (IFN gamma) in
    cells of the hematopoietic lineage and absent but inducible in non-hematopoietic cells,may be
    lysosomal in location.

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Ifi30):

    immune system

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016491 oxidoreductase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for IFI30
     Immune response Antigen presentation by MHC class II

       GeneDecks  IFI30 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for IFI30):
     hsa04612 Antigen processing and presentation

       GeneDecks  IFI30 for the pathways selected above  
    About GeneDecksing

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0019886 antigen processing and presentation of exogenous peptide antigen via MHC class II IEA--
    GO:0048147 negative regulation of fibroblast proliferation IEA--
    GO:0050821 protein stabilization IEA--
    GO:0055114 oxidation reduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for IFI30

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (IFI30)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006332

                  Sigma-Aldrich siRNA for IFI30  
                         Sigma-Aldrich shRNA for IFI30  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006332  

    REFSEQ mRNAs for IFI30 gene: 

    NM_006332.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006332  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006332
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006332
                                     untagged cDNA clone in CMV expression vector: NM_006332 

    Additional cDNA sequence: 

    AB049659.1 AF401212.1 AK123477.1 AK291551.1 BC021136.1 BC031020.1 CR604887.1 CR611745.1 
    CR617281.1 CR622428.1 CR622693.1 DQ884410.1 

    17 DOTS entries:

    DT.92357957  DT.92448454  DT.92448452  DT.100826104  DT.100826103  DT.95346751  DT.100826085  DT.100826094 
    DT.100826098  DT.92448449  DT.100826087  DT.95346753  DT.100826093  DT.121450182  DT.92448416  DT.95246806 
    DT.100826089 

    24/662 AceView cDNA sequences (see all 662 ):

    CR622693 AA381663 AK123477 AI188638 AJ572034 BE794848 CR604887 H62355 
    CA310405 AI983936 CR611745 AI355471 CB164729 BM796588 BI837864 BF992579 
    AU100505 BM051579 AI263513 BU732792 AF401212 AA995528 BQ056241 CR617281 

    highest scoring ESTs for IFI30:

    AA126780 AA128359 AA160539 AA173272 AA190455 AA190770 AA191380 AA223663 AA291182 AA291643 

    Unigene Cluster for IFI30:

    Interferon, gamma-inducible protein 30
    Hs.14623  [show with all ESTs]
    Unigene Representative Sequence: AK123477


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000407280  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    IFI30 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for IFI30

    1 / 2 / 3

    4 probe-sets matching IFI30 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    39728_at2, 3 U95-A 1 1.00 1.00 0.99 1.06 J03909 0.60 0.71 0.66 1
    925_at2, 3 U95-A 1 0.94 1.00 0.99 0.94 J03909 0.60 0.71 0.66 1

    201422_at2, 3 U133-A 1 0.82 1.00 -- -- NM_006332 0.60 1.00 0.82 1

    201422_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
    GeneDecks  IFI30 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATCAAGAATC

    SOURCE GeneReport for Unigene cluster: Hs.14623

    Expression variation in blood from EXPOLDB for IFI30

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for IFI30 gene from 5/13 species (see all 13 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC4766691   -- similar to Gamma-interferon inducible lysosomal thiol more 80(n)
    74.15(a)
    476669  XM_533874.2  XP_533874.2 
    chimpanzee
    (Pan troglodytes)
    LOC7486301   -- similar to gamma-interferon inducible lysosomal thiol more 99.6(n)
    99.6(a)
    748630  XM_001162304.1  XP_001162304.1 
    cow
    (Bos taurus)
    IFI301   -- gamma-inducible protein 30 72.95(n)
    64.75(a)
    615930  XM_868154.2  XP_873247.1 
    rat
    (Rattus norvegicus)
    Ifi301   -- interferon gamma inducible protein 30 68.84(n)
    64.49(a)
    290644  NM_001030026.1  NP_001025197.1 
    mouse
    (Mus musculus)
    Ifi301, 5 85
    interferon gamma inducible protein 301, 5 70.34(n)1
    64.08(a)1
    659721  NM_023065.31  NP_075552.21 
     AF2909735  AF3096495  (see all 15)
    About this table        Species with no ortholog for IFI30

    ENSEMBL Gene Tree for IFI30
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/47 NCBI SNPs in IFI30 are shown (see all 47 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 39)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs2732661,2
    A,C,F,H18144501(+) AGGCCT/CGGCAG 1 -- ng518Minor allele frequency- C:0.30EA NA MN EU WA 658
    rs10457471,2
    A,C,F,H18149898(+) AAGAAT/CGGTGC 1 -- ut3113Minor allele frequency- C:0.23MN EU EA WA NA 1218
    rs48081181,2
    A,C,F,H18149947(+) TAATTA/CATTTG 1 -- ng3110Minor allele frequency- N:0.00EA WA NA 720
    rs8879451,2
    A,C,F18143895(+) TTATGA/GTCCCA 1 -- ng515Minor allele frequency- G:0.21MN NA EA 546
    rs22410891,2
    A,C,F18145651(+) GCTGCG/TATGAC 1 -- ut511Minor allele frequency- T:0.12EA 1446
    rs29211,2
    A,C,F18149810(-) GTAAGT/CAGCAG 1 -- ut315Minor allele frequency- C:0.11EA NA 1578
    rs2732681,2
    C,F,H18143586(+) CACTGC/TGACGT 1 -- ng514Minor allele frequency- T:0.01EU EA WA 418
    rs2732651,2
    A,C,F18145336(+) CCACCA/GTTCCT 1 -- ng516Minor allele frequency- G:0.16NA EA MN 862
    rs48087561,2
    A,H18149004(+) GTGCAA/GTGGGG 1 M/V mis14Minor allele frequency- G:0.00EU EA WA 418
    rs72594451,2
    A,C18144011(+) CATTTA/CTTTGT 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for IFI30 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604664

    2 Novoseek disease relationships for IFI30 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    melanoma 27.67 9 18343923 (4), 12021307 (2), 16181338 (1), 15240658 (1)
    tumors 0.00 7 16181338 (1), 18343923 (1), 12021307 (1), 15240658 (1)
    About this table

    1 PharmGKB disease relationship for IFI30 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    Human Genome Epidemiology Navigator: IFI30 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/34 PubMed articles for IFI30 gene (see all 34 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10437 HGNC: 5398 AceView: IFI30 Ensembl:ENSG00000216490 euGenes: HUgn10437
    ECgene: IFI30 H-InvDB: IFI30
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for IFI30:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for IFI30 Antibodies for IFI30
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for IFI30 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search OriGene for IFI30
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for IFI30

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