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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

IFI16 Gene

protein-coding GIFtS: 57
GCID: GC01P158969

interferon, gamma-inducible protein 16

Explore 21 diseases affiliated with
IFI16 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Interferon, Gamma-Inducible Protein 16¹ ²		Gamma-Interferon-Inducible Protein 16²
IFNGIP1¹ ² ³		Interferon-Gamma Induced Protein IFI 16²
PYHIN2¹ ²		Ifi-16³
Interferon-Inducible Myeloid Differentiation Transcriptional Activator² ³

External Ids:	HGNC: 5395¹	Entrez Gene: 3428²	Ensembl: ENSG00000163565⁷	OMIM: 147586⁵	UniProtKB: Q16666³

Export aliases for IFI16 gene to outside databases

Previous GC identifers: GC01P156771 GC01P154702 GC01P155747 GC01P156196 GC01P155782 GC01P157236 GC01P130336

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for IFI16:

This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid

repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional

regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts

with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway.

Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by

RefSeq, Apr 2011)

UniProtKB/Swiss-Prot: IF16_HUMAN, Q16666

Function: Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be

involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the

regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular

proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma

protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding

and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/

AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also

involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by

recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm

recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the

activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus,

such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating

inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human

cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription

factors

Gene Wiki entry for IFI16

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000001.10 NC_018912.1 NT_004487.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the IFI16 gene promoter:
STAT1 Sp1 AP-1 ATF-2 STAT1beta STAT5A STAT1alpha AREB6 CP2 c-Jun
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): IFI16 promoter sequence

Search SABiosciences Chromatin IP Primers for IFI16

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat IFI16

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q22 Ensembl cytogenetic band: 1q23.1 HGNC cytogenetic band: 1q22

IFI16 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
IFI16 gene location

GeneLoc information about chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01P158969: view genomic region (about GC identifiers)

Start:	158,969,758 bp from pter	End:	159,024,945 bp from pter
Size:	55,188 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: IF16_HUMAN, Q16666 (See protein sequence)

Recommended Name: Gamma-interferon-inducible protein 16

Size: 785 amino acids; 88256 Da

Subunit: Forms homooligomers; isoform 2 can self-associate or associate with isoform 1 or isoform 3. Interacts with

TMEM173, AIM2, PYCARD and CASP1. Interacts with BRCA1, TP53, E2F1, RB1 and SP1

Subcellular location: Nucleus. Cytoplasm. Note=Cellular distribution is dependent on the acetylation status of the

multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization

Sequence caution: Sequence=AAF20997.1; Type=Frameshift; Positions=776; Sequence=AAF20997.1; Type=Miscellaneous

discrepancy; Note=Intron retention; Sequence=BAC04462.1; Type=Erroneous initiation; Note=Translation N-terminally

shortened; Sequence=BAD92226.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

Sequence=BAG58950.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

5 PDB 3D structures from and Proteopedia for IFI16:

2OQ0 (3D)

3B6Y (3D)

3RLN (3D)

3RLO (3D)

3RNU (3D)

Secondary accessions: B4DJT8 H3BLV7 Q59GX0 Q5T3W7 Q5T3W8 Q5T3X0 Q5T3X1 Q5T3X2 Q8N9E5 Q8NEQ7 Q96AJ5

Q9UH78

Alternative splicing: 5 isoforms: Q16666-1 Q16666-2 Q16666-3 Q16666-6 Q16666-7 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for IFI16: NX_Q16666

Post-translational modifications:

Lysine acetylation in the multipartite nuclear localization signal (NLS) regulates the subcellular location. In vitro

can be acetylated by p300/EP300 coupled to cytoplasmic localization¹

Phosphorylated on Ser and Thr¹

Isoform 3 seems to show a minor degree of complex carbohydrate addition¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q16666

IFI16 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001193496.1 NP_005522.2

ENSEMBL proteins:

ENSP00000458084 ENSP00000406406 ENSP00000407052 ENSP00000357113 ENSP00000357114

ENSP00000295809 ENSP00000352740 ENSP00000404325 ENSP00000456094 ENSP00000456129

ENSP00000394935 ENSP00000342741

Reactome Protein details: Q16666
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: IFI16 OriGene Protein Over-expression Lysate: IFI16 OriGene Custom Protein Services for IFI16
	GenScript Custom Purified and Recombinant Proteins Services for IFI16
	Novus Biologicals IFI16 Proteins Novus Biologicals IFI16 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for IFI16

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005654	nucleoplasm	IDA	14654789
GO:0005730	nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	--
GO:0016607	nuclear speck	IDA	19158679

IFI16 for ontologies About GeneDecksing

IFI16 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	Cell Signaling Technology (CST) Antibodies for IFI16
	Browse OriGene Antibodies OriGene Custom Antibody Services for IFI16
	GenScript Custom Superior Antibodies Services for IFI16
	Novus Biologicals IFI16 Antibodies
	Search for Antibodies for IFI16 at Abcam
	Uscn Antibodies for IFI16
	Search ThermoFisher Antibodies for IFI16

Assay Products for IFI16:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for IFI16
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for IFI16

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

IFI16 for domains About GeneDecksing

4 InterPro domains/families:

IPR011029 DEATH-like_dom

IPR004021 HIN200/IF120x

IPR012340 NA-bd_OB-fold

IPR004020 DAPIN

Graphical View of Domain Structure for InterPro Entry Q16666

ProtoNet protein and cluster: Q16666

2 Blocks protein families:

IPB004020 Pyrin domain

IPB004021 HIN-200/IF120x domain

UniProtKB/Swiss-Prot: IF16_HUMAN, Q16666

Domain: The HIN-20 domains mediates dsDNA binding via electrostatic interactions (PubMed:22483801)

Similarity: Belongs to the HIN-200 family

Similarity: Contains 1 DAPIN domain

Similarity: Contains 2 HIN-200 domains

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: