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IDI2 Gene

protein-coding   GIFtS: 51

GC10M001054
isopentenyl-diphosphate delta isomerase 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 5.3.3.2 3
IPPI2 1, 2, 3
OTTHUMP00000018952 2
Descriptions
IPP isomerase 2 3
Isopentenyl pyrophosphate isomerase 2 3
diphosphate dimethylallyl diphosphate isomerase 2 2
isopentenyl-diphosphate delta isomerase 2 2
External Ids
HGNC: 234871
Entrez Gene: 917342
UniProtKB: Q9BXS13
Ensembl: ENSG000001483777
Search outside databases for aliases for IDI2 gene

Previous GC identifers: GC10M001035 GC10M001018

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: IDI2_HUMAN, Q9BXS1
Function: Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to
its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the IDI2 gene  

Entrez Gene cytogenetic band: 10p15.3   Ensembl cytogenetic band:  10p15.3   HGNC cytogenetic band: 10p15.3

IDI2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M001054:     (about GC identifiers)

Start:
1,054,847 bp from pter
End:
1,061,799 bp from pter
Size:
6,953 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000010.9  NT_077567.3  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: IDI2_HUMAN, Q9BXS1 (See protein sequence)
Recommended Name: Isopentenyl-diphosphate Delta-isomerase 2  
Size: 227 amino acids; 26753 Da
Cofactor: Binds 1 magnesium ion per subunit (By similarity)
Subcellular location: Peroxisome
PDB structures from and Proteopedia :
2PNY (3D)    

REFSEQ proteins: NP_150286.1  

ENSEMBL proteins: 
ENSP00000277517 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (IDI2)
Human Recombinant Proteins from Abnova (IDI2)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005777 peroxisome IEA--
GO:0005829 cytosol EXP8806705
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Antibodies for IDI2: 
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Antibodies from Abcam (IDI2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (IDI2)
Novus Biologicals Antibodies for IDI2

Assays for IDI2: 
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Browse Kits and Assays available from Millipore
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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR011876 IsopentenylPP_isomerase_typ1
 IPR000086 NUDIX_hydrolase_core


   GeneDecks  IDI2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9BXS1

ProtoNet protein and cluster: Q9BXS1

2 Blocks protein families:
IPB000086 NUDIX hydrolase
IPB002667 Isopentenyl-diphosphate delta-isomerase


UniProtKB/Swiss-Prot: IDI2_HUMAN, Q9BXS1
Similarity: Belongs to the IPP isomerase type 1 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (IDI2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (IDI2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_033261

              Applied Biosystems Silencer® siRNAs for IDI2

              Sigma-Aldrich siRNA for IDI2  
                     Sigma-Aldrich shRNA for IDI2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for IDI2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_033261
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_033261
                                 untagged cDNA clone in CMV expression vector: NM_033261 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_033261

UniProtKB/Swiss-Prot: IDI2_HUMAN, Q9BXS1
Function: Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to
its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP)
Catalytic activity: Isopentenyl diphosphate = dimethylallyl diphosphate
Enzyme Number (IUBMB): EC 5.3.3.2 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000287 magnesium ion binding IEA--
GO:0004452 isopentenyl-diphosphate delta-isomerase activity IEA--
GO:0016787 hydrolase activity IEA--
GO:0016853 isomerase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for IDI2 (Maps provided by GeneGo):
 Cholesterol metabolism I Cholesterol Biosynthesis

   GeneDecks  IDI2 for the pathways selected above  
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  IDI2  (Your Favorite Gene powered by Ingenuity) 
 Biosynthesis of Steroids

   GeneDecks  IDI2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for IDI2):
 hsa00100 Biosynthesis of steroids
 hsa00900 Terpenoid biosynthesis

   GeneDecks  IDI2 for the pathways selected above  
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UniProtKB/Swiss-Prot: IDI2_HUMAN, Q9BXS1
Pathway: Isoprenoid biosynthesis; dimethylallyl-PP biosynthesis; dimethylallyl-PP from
isopentenyl-PP: step 1/1


5/6 Interacting proteins for IDI2 (ENSP000002775173) via UniProtKB, MINT, and/or STRING (see all 6 )

InteractantInteraction Details
GeneCardExternal ID(s)
IDI1ENSP000003707483STRING (score=.904)
FDPSENSP000003573403STRING (score=.899)
GGPS1ENSP000002828413STRING (score=.899)
MVDENSP000003010123STRING (score=.899)
SQLEENSP000002658963STRING (score=.8)
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3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006695 cholesterol biosynthetic process IEA--
GO:0008299 isoprenoid biosynthetic process IEA--
GO:0016117 carotenoid biosynthetic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for IDI2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (IDI2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (IDI2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_033261

              Sigma-Aldrich siRNA for IDI2  
                     Sigma-Aldrich shRNA for IDI2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_033261  

REFSEQ mRNAs for IDI2 gene: 

NM_033261.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_033261  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_033261
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_033261
                                 untagged cDNA clone in CMV expression vector: NM_033261 

Additional cDNA sequence: 

AF271725.1 AK056950.1 BC017778.1 

5 DOTS entries:

DT.100751051  DT.110855  DT.95272315  DT.100702798  DT.92033109 

17 AceView cDNA sequences:

AK056950 NM_033261 AA197141 BX500308 BF670552 BG184320 BC017778 BM751331 
BG183145 F00114 AF271725 AA197116 BG217172 CA313071 F00607 BG187975 
BG211295 

highest scoring ESTs for IDI2:

AK056950 BC017778 BF670552 AA197116 AF271725 BX500308 F00114 F00607 NM_033261 BF751754 

Unigene Clusters for IDI2:

Isopentenyl-diphosphate delta isomerase 2
Hs.9270  [show with all ESTs], Hs.591325  [show with all ESTs]
Unigene Representative Sequences: BC040651, BC017778


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000277517  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

IDI2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for IDI2

1 / 2 / 3

5 probe-sets matching IDI2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
60966_at2, 3 U95-C 1 1.00 1.00 0.96 1.08 AA197141 0.80 0.70 0.75 1

47538_at2, 3 U95-B 1 1.00 1.00 0.51 0.92 AI923217 0.60 0.50 0.55 1

228002_at2, 3 U133-B 1 1.00 1.00 -- -- AI814569 0.40 0.50 0.45 1

228002_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1552491_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  IDI2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTTTTCTTTT

SOURCE GeneReport for Unigene clusters: Hs.9270 Hs.591325

UniProtKB/Swiss-Prot: IDI2_HUMAN, Q9BXS1
Tissue specificity: Detected in skeletal muscle

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for IDI2 gene from 4 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
IDI21   -- isopentenyl-diphosphate delta isomerase 2 99.27(n)
98.24(a)
450262  XM_507621.2  XP_507621.2 
rat
(Rattus norvegicus)
Idi2l1   -- isopentenyl-diphosphate delta isomerase 2-like 75.33(n)
72.69(a)
689815  XM_001072122.1  XP_001072122.1 
mouse
(Mus musculus)
Idi25 13 isopentenyl-diphosphate delta isomerase 2   -- BC138100 
fruit fly
(Drosophila melanogaster)
CG59193 93D2 isopentenyl-diphosphate
delta-isomerase
42(a)   --
About this table        Species with no ortholog for IDI2

ENSEMBL Gene Tree for IDI2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for IDI2 gene
IDI12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/94 NCBI SNPs in IDI2 are shown (see all 94 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 74)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 10 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs21701421,2
A,C,F,H1054940(+) TGAGAT/CCATAC 1 -- ut31 ese37Minor allele frequency- C:0.29EU EA WA NA 532
rs107517891,2
A,C,F,H1063684(+) TATCAG/ATGAAA 1 -- ng31 tfbs36Minor allele frequency- A:0.21EU EA WA NA 600
rs172214911,2
C,F,H1055191(+) TAATCA/GAACAA 1 -- ut317Minor allele frequency- G:0.05NA EU EA WA 554
rs10442611,2
C,F,H1055710(+) CTCCCC/TAAATT 1 W/X non113Minor allele frequency- T:0.05MN NA EU EA WA 1236
rs79156781,2
C,H1063166(+) ATTTGG/ATCCTC 1 -- ng314Minor allele frequency- A:0.00EU EA WA 414
--
rs115945901,2
A,C1054792(+) TTCTCC/TGTATT 1 -- ng510--------
rs21276071,2
A,C1055132(+) TAGTCC/TGCCTG 1 -- ut31 ese30--------
--
rs125696761,2
A,C1062145(+) cccggA/Gttcgg 1 -- ng310--------
--
rs32149411,2
C1055208(+) TAAAA-/ATGGAA 1 -- ut310--------
--
rs73582161,2
C1054616(+) ggtggC/Tgggcg 1 -- ng510--------
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HapMap Linkage Disequilibrium images for IDI2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for IDI2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 91734 HGNC: 23487 AceView: IDI2 Ensembl:ENSG00000148377 euGenes: HUgn91734
ECgene: IDI2 H-InvDB: IDI2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for IDI2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 "Your Favorite Gene" Pathwaysfeedback


  
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for IDI2

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