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IDH3G Gene

protein-coding   GIFtS: 63

GC0XM152704
isocitrate dehydrogenase 3 (NAD+) gamma
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 1.1.1.41 3
H-IDHG 2
IDH-gamma 2
OTTHUMP00000025985 2
Descriptions
Isocitric dehydrogenase 2, 3
NAD (H)-specific isocitrate dehydrogenase gamma subunit 2
NAD(+)-specific ICDH 3
NAD+-specific ICDH 2
isocitrate dehydrogenase 3 (NAD+) gamma 2
isocitrate dehydrogenase, NAD(+)-specific, mitochondrial,
gamma subunit 2
External Ids
HGNC: 53861
Entrez Gene: 34212
UniProtKB: P515533
Ensembl: ENSG000000678297
Search outside databases for aliases for IDH3G gene

Previous GC identifers: GC0XM147188 GC0XM149506 GC0XM150636 GC0XM151519 GC0XM152572

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for IDH3G:
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate.
These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron
acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three
NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two
NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other
predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically
regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer
that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein
encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate
dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several
alternatively spliced transcript variants of this gene have been described, but only some of their
full length natures have been determined. [provided by RefSeq]

Gene Wiki entry for IDH3G
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the IDH3G gene  

Entrez Gene cytogenetic band: Xq28   Ensembl cytogenetic band:  Xq28   HGNC cytogenetic band: Xq28

IDH3G Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM152704:     (about GC identifiers)

Start:
152,704,415 bp from pter
End:
152,713,161 bp from pter
Size:
8,747 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000023.9  NT_011726.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: IDH3G_HUMAN, P51553 (See protein sequence)
Recommended Name: Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor  
Size: 393 amino acids; 42794 Da
Cofactor: Binds 1 magnesium or manganese ion per subunit (By similarity)
Subunit: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By
similarity)
Subcellular location: Mitochondrion
Secondary accessions: Q9BUU5

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_004126.1  NP_777358.1  


    ENSEMBL proteins: 
    ENSP00000217901 ENSP00000377366 ENSP00000359110 ENSP00000377363 ENSP00000359111 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (IDH3G)
    Human Recombinant Proteins from Abnova (IDH3G)
                  OriGene Purified Recombinant Human Protein: IDH3G 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA18029348
    GO:0005730 nucleolus IDA18029348
    GO:0005739 mitochondrion ISS--
    GO:0005759 mitochondrial matrix EXP14555658
    About this table

    Antibodies for IDH3G: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for IDH3G
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (IDH3G), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (IDH3G)
    Novus Biologicals Antibodies for IDH3G

    Assays for IDH3G: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR001804 Isocitrate/isopropylmalate_DH
     IPR004434 Isocitrate_DH_NAD_mit
     IPR019818 IsoCit/isopropylmalate_DH_CS


       GeneDecks  IDH3G for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P51553

    ProtoNet protein and cluster: P51553

    1 Blocks protein family: IPB001804 Isocitrate/isopropylmalate dehydrogenase

    UniProtKB/Swiss-Prot: IDH3G_HUMAN, P51553
    Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (IDH3G)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (IDH3G)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_174869

                  Applied Biosystems Silencer® siRNAs for IDH3G

                  Sigma-Aldrich siRNA for IDH3G  
                         Sigma-Aldrich shRNA for IDH3G  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for IDH3G
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_004135
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_004135
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_004135 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004135

    UniProtKB/Swiss-Prot: IDH3G_HUMAN, P51553
    Catalytic activity: Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH
    Enzyme regulation: Activated by increasing ADP/ATP ratios and by Ca(2+) (By similarity)
    Enzyme Number (IUBMB): EC 1.1.1.41 

    Genatlas biochemistry entry for IDH3G:
    isocitrate dehydrogenase 3,gamma subunit,NADH dependent,mitochondrial matrix,expressed in many
    tissues,catalyzing the third step of citric acid cycle,catalyzing the oxidative decarboxylation of
    isocitrate into alpha-ketoglutarate

    5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0000287 magnesium ion binding IEA--
    GO:0004449 isocitrate dehydrogenase (NAD+) activity ISS--
    GO:0005524 ATP binding IEA--
    GO:0016491 oxidoreductase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    1 Invitrogen iPath™ Online BioAtlas - Pathway for IDH3G (Maps provided by GeneGo):
     Citric acid cycle (citrate cycle

       GeneDecks  IDH3G for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  IDH3G  (Your Favorite Gene powered by Ingenuity) 
     Citrate Cycle

       GeneDecks  IDH3G for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for IDH3G):
     hsa00020 Citrate cycle (TCA cycle)

       GeneDecks  IDH3G for the pathways selected above  
    About GeneDecksing

    5/13 Interacting proteins for IDH3G (ENSP000002179013) via UniProtKB, MINT, and/or STRING (see all 13 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IDH2ENSP000003318973STRING (score=.993)
    IDH3AENSP000002995183STRING (score=.992)
    ACO2ENSP000002162543STRING (score=.953)
    ACO1ENSP000003094773STRING (score=.918)
    IDH1ENSP000002609853STRING (score=.908)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005975 carbohydrate metabolic process NAS9286695
    GO:0006099 tricarboxylic acid cycle IEA--
    GO:0006102 isocitrate metabolic process ISS--
    GO:0055114 oxidation reduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for IDH3G

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (IDH3G)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (IDH3G)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_174869

                  Sigma-Aldrich siRNA for IDH3G  
                         Sigma-Aldrich shRNA for IDH3G  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004135  NM_174869  

    REFSEQ mRNAs for IDH3G gene (2 alternative transcripts): 

    NM_004135.2   NM_174869.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004135  NM_174869  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_004135
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_004135
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_004135 

    Additional cDNA sequence: 

    AB209206.1 BC000933.2 BC001902.1 CR590006.1 CR595957.1 CR595975.1 CR599559.1 CR605410.1 
    CR606786.1 CR608068.1 CR612798.1 CR614068.1 CR615747.1 CR616395.1 CR616620.1 CR626830.1 
    U40272.1 U69268.1 U69269.1 Z68907.1 

    18 DOTS entries:

    DT.100854825  DT.97864675  DT.100854834  DT.454344  DT.100637890  DT.121309385  DT.91829470  DT.80100496 
    DT.100039177  DT.100854820  DT.100854829  DT.95105543  DT.100039176  DT.100854828  DT.100854836  DT.75151402 
    DT.95374710  DT.95142095 

    24/426 AceView cDNA sequences (see all 426 ):

    AA641973 CR606786 BQ007099 BU153610 U69268 BM680116 BQ447581 AA307345 
    AW005126 BQ448682 BM970150 CD364673 BM013648 BQ021293 BM014973 BQ301885 
    U69269 BQ008190 AW328239 CR616395 BQ446140 BM794116 CR612798 BM704640 

    highest scoring ESTs for IDH3G:

    Z68907 AL526481 AL560789 BC000933 BC001902 BE047641 BE276963 BE300119 BE389822 BE391040 

    Unigene Cluster for IDH3G:

    Isocitrate dehydrogenase 3 (NAD+) gamma
    Hs.410197  [show with all ESTs]
    Unigene Representative Sequence: AB209206


    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for IDH3G (see all 8 )

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13a · 13b · 13c · 13d
    SP1:              -     -                             -                                                                                 
    SP2:                                                  -                                                                                 
    SP3:                                                  -                                                                                 
    SP4:                                                                                                              -                     
    SP5:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for IDH3G

    5 Ensembl transcripts including schematic representations:
    ENST00000217901  ENST00000393771  ENST00000370092  ENST00000393768  ENST00000370093  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    IDH3G expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for IDH3G

    1 / 2 / 3

    5 probe-sets matching IDH3G gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    36574_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 Z68907 1.00 1.00 1.00 1

    214333_x_at2, 3 U133-A 1 1.00 1.00 -- -- U69268 0.60 1.00 0.82 1

    202471_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004135 0.60 1.00 0.82 1

    214333_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    202471_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GACAATGAGA

    SOURCE GeneReport for Unigene cluster: Hs.410197

    Expression variation in blood from EXPOLDB for IDH3G

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for IDH3G gene from 5/18 species (see all 18 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    IDH3G1   -- isocitrate dehydrogenase 3 (NAD+) gamma 90.73(n)
    96.43(a)
    481081  XM_538201.2  XP_538201.2 
    cow
    (Bos taurus)
    IDH3G1   -- isocitrate dehydrogenase 3 (NAD+) gamma 90.73(n)
    96.17(a)
    614145  NM_001076313.1  NP_001069781.1 
    rat
    (Rattus norvegicus)
    Idh3g1   -- isocitrate dehydrogenase 3 (NAD), gamma 87.62(n)
    95.93(a)
    25179  NM_031551.1  NP_113739.1 
    mouse
    (Mus musculus)
    Idh3g1, 5 X (29.50 cM)5
    isocitrate dehydrogenase 3 (NAD+), gamma1, 5 87.7(n)1
    95.17(a)1
    159291  NM_008323.11  NP_032349.11 
     AF1330935  AI3270635  (see all 19)
    zebrafish
    (Danio rerio)
    wufa01b022   -- Transcribed sequence with weak similarity to protein more 76.69(n)  57052367 
    About this table        Species with no ortholog for IDH3G

    ENSEMBL Gene Tree for IDH3G
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for IDH3G gene
    IDH3B2  IDH3A2  

    Pseudogenes
    2 related pseudogenes


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/28 NCBI SNPs in IDH3G are shown (see all 28 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 50)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr X posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20711241,2
    A,C,F,H152708802(+) CAGGGC/TCCCCT 2 -- int14Minor allele frequency- T:0.12EU EA WA 406
    rs20711231,2
    A,C,F,H152705843(+) CATCGC/AAACAG 2 -- int18Minor allele frequency- A:0.46EU EA WA NA 596
    rs22694821,2
    A,C,H152705855(+) CAGCCG/AGAGGG 2 -- int11Minor allele frequency- A:0.00EU 120
    rs174311,2
    C,F,H152709726(+) CTTCCG/ACTCTT 2 -- int115Minor allele frequency- A:0.03EU EA WA NA 962
    rs20711221,2
    C,F,H152705101(+) AGCCTG/AGGGAG 2 -- int14Minor allele frequency- A:0.45EU EA WA 416
    --
    rs37888031,2
    A,C152708185(-) GATGGA/GGTCTC 2 -- int10--------
    rs174291,2
    F,H152709366(+) GACCAC/TGTCTC 2 -- int14Minor allele frequency- T:0.08EU EA WA 418
    rs125584261,2
    C152706616(+) GGGGAC/TGGAGA 2 -- int10--------
    --
    rs174301,2
    F152709505(+) CTGGCAG/-AGAGA 2 -- int14Minor allele frequency- -:0.07NA MN 170
    rs59453511,2
    H152708520(+) CGCACC/GTCCTC 2 -- int14Minor allele frequency- G:0.00EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for IDH3G (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 300089

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for IDH3G gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 3421 HGNC: 5386 AceView: IDH3G Ensembl:ENSG00000067829 euGenes: HUgn3421
    ECgene: IDH3G H-InvDB: IDH3G
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for IDH3G:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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