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Aliases & Descriptions for IDH3A
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| | | Descriptions |
|---|
| H-IDH alpha 2 | | Isocitric dehydrogenase 2, 3 | | NAD(+)-specific ICDH 3 | | NAD(H)-specific isocitrate dehydrogenase alpha subunit 2 | | NAD+-specific ICDH 2 | | isocitrate dehydrogenase (NAD+) alpha chain 2 | | isocitrate dehydrogenase 3 (NAD+) alpha 2 | | isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial 2 |
|
| | Search outside databases for aliases for IDH3A genePrevious GC identifers: GC15P074520 GC15P071531 GC15P076017 GC15P076157 |
Summaries for IDH3A(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for IDH3A: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq] Gene Wiki entry for IDH3A |
Genomic Location for IDH3A
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the IDH3A gene 
Entrez Gene cytogenetic band: 15q25.1-q25.2 Ensembl cytogenetic band: 15q25.1 HGNC cytogenetic band: 15q25.1-q25.2IDH3A Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 15 GeneLoc Exon Structure GeneLoc location for GC15P076228:
(about GC identifiers)
Start:
|
76,228,774 bp from pter |
End:
|
76,249,939 bp from pter |
Size:
|
21,166 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000015.8 NT_010194.16
| Proteins for IDH3A
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: IDH3A_HUMAN, P50213 (See
protein sequence)Recommended Name: Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor Size: 366 amino acids; 39592 Da
Cofactor: Binds 1 magnesium or manganese ion per subunit (By similarity)
Subunit: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity)
Subcellular location: Mitochondrion
Secondary accessions: Q9H3X0Alternative splicing: 2 isoforms: P50213-1 P50213-2 Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_005521.1
ENSEMBL proteins: ENSP00000299518 ENSP00000368895
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: IDH3A 
2 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for IDH3A: Assays for IDH3A: | Protein
Domains/ Families for IDH3A(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P50213
ProtoNet protein and cluster: P50213 1 Blocks protein family: IPB001804 Isocitrate/isopropylmalate dehydrogenase
UniProtKB/Swiss-Prot: IDH3A_HUMAN, P50213Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family | Gene Function for IDH3A
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005530
Applied Biosystems Silencer® siRNAs for IDH3A
Sigma-Aldrich siRNA for IDH3A  Sigma-Aldrich shRNA for IDH3A  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005530                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_005530                                  untagged cDNA clones in CMV expression vector (see all 2): NM_005530 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_005530
UniProtKB/Swiss-Prot: IDH3A_HUMAN, P50213Catalytic activity: Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADHEnzyme Number (IUBMB): EC 1.1.1.41 Genatlas biochemistry entry for IDH3A:isocitrate dehydrogenase 3,alpha subunit,NADH dependent,mitochondrial matrix,catalyzing the third step of citric acid cycle5 Gene Ontology (GO) molecular function terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0000287 | magnesium ion binding |
IEA | -- | | GO:0004449 | isocitrate dehydrogenase (NAD+) activity |
IEA | -- | | GO:0016491 | oxidoreductase activity |
IEA | -- | | GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
IEA | -- | | GO:0030145 | manganese ion binding |
IEA | -- | About this table | Pathways & Interactions for IDH3A
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| 2 Sigma-Aldrich "Your Favorite Gene" Pathways for IDH3A (Your Favorite Gene powered by Ingenuity) 
5/12 Interacting proteins for IDH3A (P502132 ENSP000002995183) via UniProtKB, MINT, and/or STRING (see all 12
)About this table
3 Gene Ontology (GO) biological process terms (links to tree view): About this table
|
Drugs & Compounds for IDH3A(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for IDH3A
|
Transcripts for IDH3A(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005530
Sigma-Aldrich siRNA for IDH3A  Sigma-Aldrich shRNA for IDH3A  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_005530 REFSEQ mRNAs for IDH3A gene: NM_005530.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_005530               OriGene GFP tagged cDNA clone in CMV expression vector: NM_005530                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_005530                                  untagged cDNA clones in CMV expression vector (see all 2): NM_005530  Additional cDNA sequence: AK093531.1 AK096721.1 AK123316.1 AK222973.1 AK296002.1 AK299970.1 AK315963.1 AK316051.1 AL110096.1 AL442090.1 BC021967.2 BC036227.1 CR599067.1 CR624257.1 CR749548.1 U07681.1 24/25 DOTS entries (see all 25
): DT.86855063 DT.99945419 DT.100824942 DT.100824943 DT.100824944 DT.95258957 DT.92443593 DT.100824933 DT.91830964 DT.100824945 DT.100027192 DT.95197969 DT.95363838 DT.121032158 DT.121032190 DT.121032180 DT.430952 DT.100027191 DT.121032125 DT.121032155 DT.121032166 DT.99931674 DT.100714103 DT.101983174 24/340 AceView cDNA sequences (see all 340
):AV707486 BP363836 CB131647 BM719480 AL043545 AA095501 BE273577 CB159510 AU160420 CB216934 CR599067 CR624257 AI424108 BE550193 BI261860 AA911181 BP352536 BF111986 BP358032 BQ643334 AK123316 BM838562 AA878128 BQ011986
highest scoring ESTs for IDH3A:U07681 AA127955 AA128045 AA130890 AA150840 AA151012 AA228279 AA229668 AA417572 AA417949 Unigene Cluster for IDH3A: Isocitrate dehydrogenase 3 (NAD+) alpha Hs.591110 [show with all ESTs]Unigene Representative Sequence: AK123316
GeneLoc Exon Structure
5/20 Alternative Splicing Database (ASD) splice patterns (SP) for IDH3A (see all 20
)
| ExUns: | 1a | · | 1b | · | 1c | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6a | · | 6b | ^ | 7a | · | 7b | ^ | 8 | ^ | 9a | · | 9b | ^ | 10a | · | 10b | · | 10c | ^ | 11a | · | 11b | · | 11c | ^ | 12a | · | 12b | · | 12c | · | 12d | · | 12e | ^ | |
| SP1: | |   | |   | |   | - |   | - |   | |   | |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | - |   | - |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | - |   | - |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | - |   | - |   | |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | - |   | |   | |   | - |   | |   | |   | |   | |   | |   | - |   | |   | |   | - |   | - |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | - |   | - |   | |   | |   | |   |
| ExUns: | 13 | ^ | 14 | ^ | 15a | · | 15b | ^ | 16a | · | 16b | |
| SP1: | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for IDH3A
2 Ensembl transcripts including schematic representations: ENST00000299518
ENST00000379576
|
Expression for IDH3A
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| IDH3A expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for IDH3A
1 / 2 / 3 5 probe-sets matching IDH3A gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: GCCTCCTTTG
SOURCE GeneReport for Unigene cluster: Hs.591110
Expression variation in blood from EXPOLDB for IDH3A |
Orthologs for IDH3A
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for IDH3A gene from 5/27 species (see all 27
)
About this table Species with no ortholog for IDH3A
ENSEMBL Gene Tree for IDH3A | Paralogs for IDH3A(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for IDH3A gene
- IDH3G2 IDH3B2
|
SNPs/Variants for IDH3A(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for IDH3A (up to first 250kb)
|
Disorders & Mutations for IDH3A
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 601149
|
Medical News for IDH3A(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for IDH3A (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/16 PubMed articles for IDH3A gene (see all 16
):- Characterization of a cDNA clone for human NAD(+)-specific isocitrate dehydrogenase alpha-subunit and structural comparison with its isoenzymes from different species. (PubMed id 7755589)1, 3, 4 Kim Y.O.... Huh T.L. (1995)
- Assignment of the human mitochondrial NAD+ -specific isocitrate dehydrogenase alpha subunit (IDH3A) gene to 15q25.1-->q25.2by in situ hybridization. (PubMed id 8833160)1, 2, 3 Huh T.L....Inazawa J. (1996)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase. (PubMed id 17432878)3 Bzymek K.P. and Colman R.F. (2007)
- The full-ORF clone resource of the German cDNA Consortium. (PubMed id 17974005)4 Bechtel S....Schupp I. (2007)
- Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase. (PubMed id 16737955)3 Soundar S....Colman R.F. (2006)
- Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress. (PubMed id 16236267)3 Guo D.... Wang C.-Y. (2005)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)3 Ota T....Sugano S. (2004)
- Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase. (PubMed id 14555658)3 Soundar S....Colman R.F. (2003)
- The human plasma proteome: history, character, and diagnostic prospects. (PubMed id 12488461)3 Anderson N.L. and Anderson N.G. (2002)
|
Search for IDH3A
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing IDH3A
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing IDH3A
(According to HUGE)
About This Section
| -- |
Specialized Databases showing IDH3A(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for IDH3A(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
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