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IDE Gene

protein-coding   GIFtS: 72
GCID: GC10M094211

Insulin-Degrading Enzyme

  See IDE-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Insulin-Degrading Enzyme1 2     INSULYSIN2
Insulin Protease2 3     insulinase2
Abeta-Degrading Protease2 3     Insulinase3
EC 3.4.24.563 8     Insulysin3
insulysin1     EC 3.4.248

External Ids:    HGNC: 53811   Entrez Gene: 34162   Ensembl: ENSG000001199127   OMIM: 1466805   UniProtKB: P147353   

Export aliases for IDE gene to outside databases

Previous GC identifers: GC10M093095 GC10M093435 GC10M094342 GC10M093878 GC10M094201 GC10M087837


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for IDE Gene:
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins
activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as
glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in
insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this
protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this
gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm
but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion.
Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript
variants have been described but have not been experimentally verified.(provided by RefSeq, Sep 2009)

GeneCards Summary for IDE Gene:
IDE (insulin-degrading enzyme) is a protein-coding gene. Diseases associated with IDE include type 2 diabetes mellitus, and alzheimer's disease. GO annotations related to this gene include ATPase activity and protein homodimerization activity. An important paralog of this gene is NRD1.

UniProtKB/Swiss-Prot: IDE_HUMAN, P14735
Function: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other
peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP.
May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and
microglia

Gene Wiki entry for IDE (Insulin-degrading enzyme) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000010.10  NT_030059.14  NC_018921.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the IDE gene promoter:
         E2F-4   E2F-5   NF-YC   CBF-C   CBF-A   CBF-B   CP1A   CP1C   NF-Y   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidIDE promoter sequence
   Search Chromatin IP Primers for IDE

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat IDE


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q23-q25   Ensembl cytogenetic band:  10q23.33   HGNC cytogenetic band: 10q23-q25

IDE Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
IDE gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M094211:  view genomic region     (about GC identifiers)

Start:
94,211,441 bp from pter      End:
94,333,852 bp from pter
Size:
122,412 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: IDE_HUMAN, P14735 (See protein sequence)
Recommended Name: Insulin-degrading enzyme  
Size: 1019 amino acids; 117968 Da
Cofactor: Binds 1 zinc ion per subunit
Subunit: Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV)
envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for
this virus
Miscellaneous: ATP-binding induces a conformation change
Selected PDB 3D structures from and Proteopedia for IDE (see all 28):
2G47 (3D)        2G48 (3D)        2G49 (3D)        2G54 (3D)        2G56 (3D)        2JBU (3D)    
Secondary accessions: B2R721 B7ZAU2 D3DR35 Q5T5N2
Alternative splicing: 2 isoforms:  P14735-1   P14735-2   

Explore the universe of human proteins at neXtProt for IDE: NX_P14735

Explore proteomics data for IDE at MOPED

Post-translational modifications: 

  • The N-terminus is blocked1
  • Ubiquitination2 at Lys192, Lys308, Lys884, Lys929, Lys938, Lys1018
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for IDE (P14735) (see all 7)
     YYSSNLM  EHMLFLGT  KVLLISDP  KIVPIKDIR 


    See IDE Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001159418.1  NP_004960.2  

    ENSEMBL proteins: 
     ENSP00000265986   ENSP00000473506   ENSP00000408850   ENSP00000360637  

    IDE Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for IDE
    OriGene Protein Over-expression Lysate for IDE
    OriGene MassSpec for IDE
    OriGene Custom Protein Services for IDE
    GenScript Custom Purified and Recombinant Proteins Services for IDE
    Novus Biologicals IDE Proteins
    Novus Biologicals IDE Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for IDE

     
    Search eBioscience for Proteins for IDE 

     
    antibodies-online proteins for IDE (7 products) 

     
    antibodies-online peptides for IDE

    IDE Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of IDE
    R&D Systems Antibodies for IDE (Insulysin/IDE)
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    Abcam antibodies for IDE
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    ThermoFisher Antibodies for IDE
    antibodies-online antibodies for IDE (72 products) 

    IDE Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for IDE
    Browse R&D Systems for biochemical assays
    GenScript IDE-Activity-based Protease?src=genec Assay for Compound Screening
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for IDE
    Cloud-Clone Corp. CLIAs for IDE
    Search eBioscience for ELISAs for IDE 
    antibodies-online kits for IDE (38 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    5 InterPro protein domains:
     IPR001431 Pept_M16_Zn_BS
     IPR011765 Pept_M16_N
     IPR011237 Pept_M16_dom
     IPR007863 Peptidase_M16_C
     IPR011249 Metalloenz_LuxS/M16

    Graphical View of Domain Structure for InterPro Entry P14735

    ProtoNet protein and cluster: P14735

    1 Blocks protein domain: IPB001431 Insulinase-like peptidase

    UniProtKB/Swiss-Prot: IDE_HUMAN, P14735
    Domain: The SlyX motif may be involved in the non-conventional secretion of the protein (By similarity)
    Similarity: Belongs to the peptidase M16 family


    Find genes that share domains with IDE           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: IDE_HUMAN, P14735
    Function: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other
    peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP.
    May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and
    microglia
    Catalytic activity: Degradation of insulin, glucagon and other polypeptides. No action on proteins
    Enzyme regulation: Activated by small peptides (By similarity). Activated by ATP and GTP, and to a lesser extent
    by CTP, TTP and PPPi. Inhibited by bacitracin. Inhibited by S-nitrosylation and oxidation agents

         Genatlas biochemistry entry for IDE:
    insulin degrading enzyme,cytosolic metalloproteinase (110kDa)

         Enzyme Numbers (IUBMB): EC 3.4.24.561 2 EC 3.4.242

         Gene Ontology (GO): Selected molecular function terms (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001540beta-amyloid binding IEA--
    GO:0001948glycoprotein binding IPI17055432
    GO:0003824catalytic activity ----
    GO:0004222metalloendopeptidase activity IDA17051221
    GO:0005102receptor binding IPI--
         
    Find genes that share ontologies with IDE           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for IDE:
     Increased resistance to Trastu 

         7 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Ide):
     behavior/neurological  hematopoietic system  homeostasis/metabolism  immune system  nervous system 
     other  renal/urinary system 

    Find genes that share phenotypes with IDE           About GenesLikeMe

    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for IDE

    miRNA
    Products:
        
    miRTarBase miRNAs that target IDE:
    hsa-mir-7-5p (MIRT025720)

    Block miRNA regulation of human, mouse, rat IDE using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate IDE (see all 59):
    hsa-miR-576-3p hsa-miR-193a-3p hsa-miR-29a hsa-miR-200a hsa-miR-30d hsa-miR-141 hsa-miR-30a hsa-miR-1184
    SwitchGear 3'UTR luciferase reporter plasmidIDE 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for IDE
    Predesigned siRNA for gene silencing in human, mouse, rat IDE

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for IDE

    Clone
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    OriGene clones in human, mouse for IDE (see all 12)
    OriGene ORF clones in mouse, rat for IDE
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: IDE (NM_001279)
    Sino Biological Human cDNA Clone for IDE
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for IDE
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat IDE

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for IDE 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for IDE


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    IDE_HUMAN, P14735: Cytoplasm. Cell membrane. Secreted (By similarity). Note=Present at the cell surface of
    neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol5
    extracellular5
    mitochondrion5
    nucleus5
    peroxisome5
    plasma membrane5
    endoplasmic reticulum1
    endosome1

    Gene Ontology (GO): Selected cellular component terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005615extracellular space IDA9830016
    GO:0005634NOT nucleus IDA18226493
    GO:0005737cytoplasm IDA18226493
    GO:0005739mitochondrion IDA15285718
    GO:0005777peroxisome IDA9231799

    Find genes that share ontologies with IDE           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for IDE About    
    See pathways by source

    SuperPathContained pathways About
    1Alzheimer's disease
    Alzheimers Disease0.44
    Alzheimer's disease0.44
    2Apoptotic Pathways in Synovial Fibroblasts
    DHA Signaling0.74
    3A-beta Uptake and Degradation
    A-beta Uptake and Degradation
    4A-beta Plaque Formation and APP Metabolism
    A-beta Plaque Formation and APP Metabolism


    Find genes that share SuperPaths with IDE           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 R&D Systems Pathways for IDE
        A-beta Uptake & Degradation
    A-beta Plaque Formation & APP Metabolism

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for IDE
        DHA Signaling

    1 BioSystems Pathway for IDE
        Alzheimers Disease



    1 Kegg Pathway  (Kegg details for IDE):
        Alzheimer's disease

        Pathway & Disease-focused RT2 Profiler PCR Arrays including IDE: 
              Alzheimer's Disease in human mouse rat
              Diabetes in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for IDE

    STRING Interaction Network Preview (showing 5 interactants - click image to see 24)

    Selected Interacting proteins for IDE (P147351, 2, 3 ENSP000002659864) via UniProtKB, MINT, STRING, and/or I2D (see all 45)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CCL3P101471, 2, 3, ENSP000002252454EBI-2556886,EBI-8459634 MINT-8087623 MINT-8087747 MINT-8087595 I2D: score=1 STRING: ENSP00000225245
    ENSG00000204221O152133I2D: score=1 
    ENSG00000206284O152133I2D: score=1 
    ENSG00000226916O152133I2D: score=1 
    ENSG00000236222O152133I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 19):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006200ATP catabolic process ----
    GO:0006508proteolysis IDA--
    GO:0008286insulin receptor signaling pathway NAS3059494
    GO:0008340determination of adult lifespan IDA18448515
    GO:0010815bradykinin catabolic process IDA17613531

    Find genes that share ontologies with IDE           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for IDE

    1 HMDB Compound for IDE    About this table
    CompoundSynonyms CAS #PubMed Ids
    ZincZinc (see all 2)7440-66-6--

    3 DrugBank Compounds for IDE    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Insulin, porcine-- 9004-14-2target--13560210 17016423 17139284 8504733 9643444
    Bacitracin-- 1405-87-4targetinhibitor8484964 11752352
    Insulin Regular -- 11061-68-0target--17139284 17016423

    Selected Novoseek inferred chemical compound relationships for IDE gene (see all 25)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    thermolysin 59.2 4 7738784 (2), 8104941 (1), 7806544 (1)
    chymostatin 53.7 2 7494830 (1), 7596985 (1)
    1,10-phenanthroline 48.2 5 7789307 (1), 7494830 (1), 8024692 (1), 7738784 (1) (see all 5)
    zinc 46.7 25 7806544 (4), 18621727 (2), 8104941 (1), 9128138 (1) (see all 13)
    thimet 46.5 3 11809755 (1)
    n-ethylmaleimide 46.3 5 7494830 (1), 8300632 (1), 7596985 (1), 18986166 (1)
    triphosphate 40.3 5 15749695 (2), 15494400 (1), 16723115 (1)
    p-chloromercuribenzoate 40.2 1 7494830 (1)
    leupeptin 37.1 2 7494830 (1), 7596985 (1)
    diisopropylfluorophosphate 28.1 1 7494830 (1)



    Find genes that share compounds with IDE           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for IDE gene (2 alternative transcripts): 
    NM_001165946.1  NM_004969.3  

    Unigene Cluster for IDE:

    Insulin-degrading enzyme
    Hs.500546  [show with all ESTs]
    Unigene Representative Sequence: NM_004969
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000265986(uc001kia.3) ENST00000496903 ENST00000478361 ENST00000463640
    ENST00000462988 ENST00000492362 ENST00000436178 ENST00000371581(uc010qnp.2 uc001khz.3)

    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate IDE (see all 59):
    hsa-miR-576-3p hsa-miR-193a-3p hsa-miR-29a hsa-miR-200a hsa-miR-30d hsa-miR-141 hsa-miR-30a hsa-miR-1184
    SwitchGear 3'UTR luciferase reporter plasmidIDE 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat IDE
    Clone
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: IDE (NM_001279)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for IDE
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat IDE
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for IDE
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat IDE
      QuantiTect SYBR Green Assays in human, mouse, rat IDE
      QuantiFast Probe-based Assays in human, mouse, rat IDE

    Additional mRNA sequence: 

    AB209204.1 AK093287.1 AK302578.1 AK312810.1 AK316407.1 BC036188.1 BC096336.1 BC096337.1 
    BC096338.1 BC096339.3 BX648462.1 M21188.1 

    10 DOTS entries:

    DT.101980937  DT.99964461  DT.454997  DT.92434686  DT.121290466  DT.121290448  DT.101980938  DT.121290477 
    DT.313438  DT.95278073 

    Selected AceView cDNA sequences (see all 208):

    AW612893 CB112462 BG675022 BM680093 CF529435 BM797559 BM701269 BM716781 
    AI624294 AA057839 BP352517 AU130876 AA458827 CD655769 AA732354 BM996916 
    AI796011 AA862928 AI624096 AI141765 BU618422 AA609313 AW169670 CA945025 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for IDE    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25 ^ 26 ^
    SP1:                                                                                -                             -                                             
    SP2:                                                                                                        -                                                   
    SP3:                                                                                                                                                            

    ExUns: 27 ^ 28 ^ 29a · 29b
    SP1:                        
    SP2:                        
    SP3:                        


    ECgene alternative splicing isoforms for IDE

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    IDE expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGACACAGCC
    IDE Expression
    About this image


    IDE expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 11) fully expand
     
     Brain (Nervous System)    fully expand to see all 6 entries
             Cerebral Cortex
     
     Neural Tube (Nervous System)    fully expand to see all 3 entries
             Metencephalon
     
     Gut Tube (Gastrointestinal Tract)    fully expand to see all 2 entries
             Hindgut
     
     Kidney (Urinary System)    fully expand to see all 2 entries
             Metanephros
     
     Lung (Respiratory System)    fully expand to see all 2 entries
             Stem Bronchi
    IDE Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    IDE Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.500546
        Pathway & Disease-focused RT2 Profiler PCR Arrays including IDE: 
              Alzheimer's Disease in human mouse rat
              Diabetes in human mouse rat

    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for IDE
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat IDE
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    QuantiFast Probe-based Assays in human, mouse, rat IDE
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for IDE

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for IDE gene from Selected species (see all 25)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ide1 , 5 insulin degrading enzyme1, 5 89.01(n)1
    95.09(a)1
      19 (32.24 cM)5
    159251  NM_031156.31  NP_112419.31 
     372687435 
    chicken
    (Gallus gallus)
    Aves IDE1 insulin-degrading enzyme 82.7(n)
    92.64(a)
      423814  XM_421686.4  XP_421686.4 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    Uncharacterized protein
    88(a)
    87(a)
    many → 1
    many → 1
    GL343262.1(1888820-1946765)
    GL344649.1(794-25227)
    African clawed frog
    (Xenopus laevis)
    Amphibia BJ098956.12   -- 77.72(n)    BJ098956.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.55542 Transcribed sequence with moderate similarity to protein more 77.81(n)    CF348565.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Ide1 , 3 proteolysis and peptidolysis insulysin3
    Insulin degrading metalloproteinase1
    46(a)3
    52.85(n)1
    47.08(a)1
      77B83
    402481  NM_079458.41  NP_524182.31 
    worm
    (Caenorhabditis elegans)
    Secernentea C28F5.43
    F44E7.41
    insulinase3
    F44E7.41
    47(a)
    (best of 4)3
    49.98(n)1
    43.5(a)1
      II(7520107-7523295)3
    1789661  NM_171461.31  NP_741542.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes STE23(YLR389C)4
    STE231
    Metalloprotease involved, with homolog Axl1p, in N-terminal more4
    STE231
    49.2(n)1
    39.98(a)1
      12(902660-899577)4
    8511051, 4  NP_013493.21, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT2G417901 AT2G41790 49.34(n)
    39.32(a)
      818778  NM_129743.3  NP_181710.1 
    rice
    (Oryza sativa)
    Liliopsida Os07g05703001 Os07g0570300 49.48(n)
    40.58(a)
      4343659  NM_001066579.1  NP_001060044.1 


    ENSEMBL Gene Tree for IDE (if available)
    TreeFam Gene Tree for IDE (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for IDE gene
    NRD12  
    1 SIMAP similar gene for IDE using alignment to 3 protein entries:     IDE_HUMAN (see all proteins):
    NRD1

    Find genes that share paralogs with IDE           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for IDE
    PGOHUM00000258502


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for IDE (see all 2441)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 10 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1859845061,2
    C--87858104(+) GAATTC/TTTTTT 2 -- int10--------
    rs38312741,2
    C--87865074(-) CACAC-/AC/CA 
            
    TCACA
    2 -- int10--------
    rs784768931,2
    C--87865091(+) GTGTGC/TGCATG 2 -- int10--------
    rs747139511,2
    C--87865092(+) TGTGTA/GCATGC 2 -- int10--------
    rs339175541,2
    C--87894019(+) AAAGAA/-AAAAA 1 -- int11Minor allele frequency- -:0.00NA 2
    rs1881574231,2
    --87896222(+) CACATA/GTATGA 1 -- int10--------
    rs1384008291,2
    C--87896248(+) CTCAC-/ATAT  
            
    ATGAT
    1 -- int10--------
    rs1471705851,2
    C--87896249(+) TCACA-/ATAT  
            
    TGATA
    1 -- int10--------
    rs1838310911,2
    C--87897160(+) CTCAAA/TTTTTT 1 -- int10--------
    rs723461011,2
    C--87898408(+) AACCT-/AC    
       A
    /CAC
    AACAG
    3 -- int1 cds10--------

    HapMap Linkage Disequilibrium report for IDE (94211441 - 94333852 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for IDE:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv508600CNV Loss20534489
    nsv895894CNV Loss21882294
    nsv895895CNV Gain21882294

    Human Gene Mutation Database (HGMD): IDE
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing IDE
    DNA2.0 Custom Variant and Variant Library Synthesis for IDE

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 146680    OMIM disorders: --

    2 diseases for IDE:    
    About MalaCards
    type 2 diabetes mellitus    alzheimer's disease

    1 disease from the University of Copenhagen DISEASES database for IDE:
    Alzheimer's disease

    Find genes that share disorders with IDE           About GenesLikeMe

    Selected Novoseek inferred disease relationships for IDE gene (see all 18)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    alzheimers disease 78.8 100 10213160 (2), 15277615 (2), 16894402 (2), 16876916 (2) (see all 51)
    senile plaques 67.5 9 10213160 (1), 16876916 (1), 17244626 (1), 19770104 (1) (see all 6)
    late onset alzheimer disease 61.9 1 20098734 (1)
    amyloid deposition 49.7 6 12941771 (1), 17047308 (1), 19073193 (1), 15750394 (1)
    neurodegeneration 40.1 1 19606063 (1)
    hyperinsulinemia 37.9 7 12634421 (3), 19396426 (1), 11306609 (1), 15850385 (1)
    insulin resistance 37.6 10 16380485 (2), 16930758 (1), 16894402 (1), 17026490 (1) (see all 7)
    niddm 34.8 17 18621727 (1), 12634421 (1), 2246195 (1), 15850385 (1) (see all 8)
    hypofunction 33.3 6 12634421 (2), 15850385 (1)
    dementia 28.4 12 17192720 (5), 19415148 (3), 18644361 (1)

    Genetic Association Database (GAD): IDE
    Human Genome Epidemiology (HuGE) Navigator: IDE (102 documents)

    Export disorders for IDE gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for IDE gene, integrated from 10 sources (see all 321):
    (articles sorted by number of sources associating them with IDE)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes. (PubMed id 2293021)1, 2, 3, 9 Affholter J.A.... Roth R.A. (Mol. Endocrinol. 1990)
    2. Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE. (PubMed id 17613531)1, 2, 9 Im H.... Tang W.-J. (J. Biol. Chem. 2007)
    3. Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism. (PubMed id 17051221)1, 2, 9 Shen Y.... Tang W.-J. (Nature 2006)
    4. Quantitative trait loci near the insulin-degrading enzyme (IDE) gene contribute to variation in plasma insulin levels. (PubMed id 15277398)1, 4, 9 Gu H.F....Prince J.A. (Diabetes 2004)
    5. Molecular bases for the recognition of short peptide substrates and cysteine-directed modifications of human insulin-degrading enzyme. (PubMed id 18986166)1, 2, 9 Malito E....Tang W.-J. (Biochemistry 2008)
    6. Concordant association of insulin degrading enzyme gene (IDE) variants with IDE mRNA, Abeta, and Alzheimer's disease. (PubMed id 20098734)1, 4, 9 Carrasquillo M.M....Morgan K. (PLoS ONE 2010)
    7. Polymorphisms within insulin-degrading enzyme (IDE) gene determine insulin metabolism and risk of type 2 diabetes. (PubMed id 19809796)1, 4, 9 Rudovich N....Pfeiffer A.F. (J. Mol. Med. 2009)
    8. High-density haplotype structure and association testing of the insulin-degrading enzyme (IDE) gene with type 2 diabetes in 4,206 people. (PubMed id 16380485)1, 4, 9 Florez J.C....Groop L. (Diabetes 2006)
    9. Insulin degrading enzyme is a cellular receptor mediating varicella- zoster virus infection and cell-to-cell spread. (PubMed id 17055432)1, 2, 9 Li Q.... Cohen J.I. (Cell 2006)
    10. Insulin-degrading enzyme and Alzheimer disease: a genetic association study in the Han Chinese. (PubMed id 15277615)1, 4, 9 Bian L....He L. (Neurology 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 3416 HGNC: 5381 AceView: IDE Ensembl:ENSG00000119912 euGenes: HUgn3416
    ECgene: IDE Kegg: 3416 H-InvDB: IDE

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for IDE Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for IDE gene:
    Search GeneIP for patents involving IDE

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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