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ID4 Gene

protein-coding   GIFtS: 59

GC06P019945
inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
IDB4 2
OTTHUMP00000016081 2
bHLHb27 1, 2
Descriptions
Inhibitor of DNA binding 4 3
inhibitor of DNA binding 4, dominant negative
helix-loop-helix protein 2
External Ids
HGNC: 53631
Entrez Gene: 34002
UniProtKB: P479283
Ensembl: ENSG000001722017
Search outside databases for aliases for ID4 gene

Previous GC identifer: GC06P019895

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for ID4:
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of
tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the
bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH
proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but
lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and
transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995
[PubMed 7665172]).[supplied by OMIM]

UniProtKB/Swiss-Prot: ID4_HUMAN, P47928
Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able
to form heterodimers with other HLH proteins, thereby inhibiting DNA binding

Gene Wiki entry for ID4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ID4 gene  

Entrez Gene cytogenetic band: 6p22-p21   Ensembl cytogenetic band:  6p22.3   HGNC cytogenetic band: 6p22.3

ID4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P019945:     (about GC identifiers)

Start:
19,945,596 bp from pter
End:
19,948,894 bp from pter
Size:
3,299 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000006.10  NT_007592.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ID4_HUMAN, P47928 (See protein sequence)
Recommended Name: DNA-binding protein inhibitor ID-4  
Size: 161 amino acids; 16622 Da
Subunit: Heterodimer with other HLH proteins
Subcellular location: Nucleus
Secondary accessions: Q13005

REFSEQ proteins: NP_001537.1  

ENSEMBL proteins: 
ENSP00000367972 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (ID4)
Human Recombinant Proteins from Abnova (ID4)
              OriGene Purified Recombinant Human Protein: ID4 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
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Antibodies for ID4: 
Browse Antibodies Central at Invitrogen
Millipore Mono- and Polyclonal Antibodies for the study of ID4
Sigma-Aldrich Antibodies for ID4
Browse R&D Systems for Antibodies
Antibodies from Abcam (ID4), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (ID4)
Novus Biologicals Antibodies for ID4

Assays for ID4: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR001092 HLH_basic


   GeneDecks  ID4 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P47928

ProtoNet protein and cluster: P47928

1 Blocks protein family: IPB001092 Basic helix-loop-helix dimerization domain bHLH

UniProtKB/Swiss-Prot: ID4_HUMAN, P47928
Similarity: Contains 1 basic helix-loop-helix (bHLH) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ID4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ID4)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001546

              Applied Biosystems Silencer® siRNAs for ID4

              Sigma-Aldrich siRNA for ID4  
                     Sigma-Aldrich shRNA for ID4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for ID4
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001546
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001546
                                 untagged cDNA clone in CMV expression vector: NM_001546 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001546

UniProtKB/Swiss-Prot: ID4_HUMAN, P47928
Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able
to form heterodimers with other HLH proteins, thereby inhibiting DNA binding

Genatlas biochemistry entry for ID4:
inhibitor of DNA binding 4,mouse Id-4 related,antagonist of MyoD activity or creatine kinase
enhancer,mainly expressed in thyroid,brain and fetal tissue and in some nervous system tumor cell
lines

5 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Id4):

behavior/neurologicalgrowth/sizelethality-postnatallife span-post-weaning/agingnervous system

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003714 transcription corepressor activity TAS7665172
GO:0005515 protein binding IPI17474147
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Kegg Pathway  (Kegg details for ID4):
 hsa04350 TGF-beta signaling pathway

   GeneDecks  ID4 for the pathways selected above  
About GeneDecksing

5/16 Interacting proteins for ID4 (P479281 ENSP000003679723) via UniProtKB, MINT, and/or STRING (see all 16 )
InteractantInteraction Details
GeneCardExternal ID(s)
CRKP461081EBI-1754719, EBI-886
GRB2P629931EBI-1754719, EBI-401755
NCK1P163331EBI-1754719, EBI-389883
PIK3R1P279861EBI-1754719, EBI-79464
PLCG1P191741EBI-1754719, EBI-79387
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5/10 Gene Ontology (GO) biological process terms (links to tree view) (see all 10 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle IEA--
GO:0006357 regulation of transcription from RNA polymerase II promoter TAS7665172
GO:0007405 neuroblast proliferation IEA--
GO:0007420 brain development IEA--
GO:0008284 positive regulation of cell proliferation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ID4

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ID4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ID4)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001546

              Sigma-Aldrich siRNA for ID4  
                     Sigma-Aldrich shRNA for ID4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001546  

REFSEQ mRNAs for ID4 gene: 

NM_001546.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001546  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001546
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001546
                                 untagged cDNA clone in CMV expression vector: NM_001546 

Additional cDNA sequence: 

AF085889.1 AJ420553.1 AK130851.1 BC014941.1 U16153.1 U28368.1 Y07958.1 

5 DOTS entries:

DT.115783  DT.75101421  DT.121344012  DT.87002090  DT.443817 

24/358 AceView cDNA sequences (see all 358 ):

BU743053 AA371177 BF448042 BF058198 F13013 BQ721197 BM703639 AA384132 
AA972772 BE467572 AI951466 AA405766 AI887913 AI668810 BM473615 AI671882 
BE219449 AW119000 AW297589 BE669883 AW451803 AA454080 BF941891 BU736386 

highest scoring ESTs for ID4:

AA001053 AA228265 AA230021 AA283186 AA292346 AA332060 AA360044 AA371177 AA384132 AA394164 

Unigene Cluster for ID4:

Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Hs.519601  [show with all ESTs]
Unigene Representative Sequence: NM_001546


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000378700  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ID4 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for ID4

1 / 2 / 3

10 probe-sets matching ID4 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
41536_at*2, 3 U95-A 1 1.00 1.00 0.99 0.78 AL022726 0.20 1.00 0.72 1

54939_at*2, 3 U95-C 1 1.00 1.00 0.81 1.27 AI668810 0.80 1.00 0.91 1

209293_x_at2, 3 U133-A 1 1.00 1.00 -- -- U16153 0.60 1.00 0.82 1

209291_at2, 3 U133-A 1 1.00 1.00 -- -- AW157094 0.80 1.00 0.91 1

229386_at*2, 3 U133-B 1 1.00 1.00 -- -- AW131801 0.60 1.00 0.82 1

209292_at*2, 3 U133-A 1 1.00 1.00 -- -- AL022726 0.20 1.00 0.72 1

209293_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209291_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

229386_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209292_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  ID4 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGAATATTAA

SOURCE GeneReport for Unigene cluster: Hs.519601

Expression variation in blood from EXPOLDB for ID4

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for ID4 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
ID41   -- inhibitor of DNA binding 4, dominant negative helix-loop-helix more 98.19(n)
96.6(a)
747716  XM_001170946.1  XP_001170946.1 
rat
(Rattus norvegicus)
Id41   -- inhibitor of DNA binding 4 92.96(n)
95.65(a)
291023  NM_175582.1  NP_783172.1 
mouse
(Mus musculus)
Id41, 5 13 (31.00 cM)5
inhibitor of DNA binding 41, 5 93.17(n)1
98.14(a)1
159041  NM_031166.21  NP_112443.11 
 AF0778595  AJ0019695  (see all 14)
chicken
(Gallus gallus)
ID41   -- inhibitor of DNA binding 4, dominant negative helix-loop-helix more 80.91(n)
83.87(a)
374150  NM_204282.1  NP_989613.1 
zebrafish
(Danio rerio)
zgc:1232141   -- zgc:123214 66.13(n)
69.6(a)
664761  NM_001039990.1  NP_001035079.1 
About this table        Species with no ortholog for ID4

ENSEMBL Gene Tree for ID4
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for ID4 gene
ID12  ID22  ID32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/54 NCBI SNPs in ID4 are shown (see all 54 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 6 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs77658931,2
A,C,F,H19949367(+) TGTTAT/CTTCCT 1 -- ng314Minor allele frequency- C:0.41EU EA WA 410
rs10470331,2
C,F,H19948471(-) AGATTT/CCGTAA 1 -- ut3119Minor allele frequency- C:0.19EA NS MN EU WA 3032
--
rs10473161,2
A,C19945945(-) GCTCTA/GCGCGA 1 -- ut51 ese30--------
rs69198581,2
A,C19943994(+) CAGGAA/GAAAGG 1 -- ng51 trp30--------
--
rs126622341,2
A,C19945948(+) CGTAGA/GGCGCA 1 -- ut510--------
rs9263691,2
C19945090(-) TGCCAC/TTCCTA 1 -- ng510--------
--
rs117591021,2
C19945753(+) GGGCTC/TGGGCT 1 -- ut51 ese30--------
rs82141,2
F19947758(+) AGAAGC/TCTACT 1 -- ut312Minor allele frequency- T:0.18MN 372
rs74528071,2
H19944956(+) GGCGAA/TTAATG 1 -- ng514Minor allele frequency- T:0.00EU EA WA 406
rs37983391,2
C19948974(-) AAATAC/TTACCT 1 -- ng310--------
About this table

HapMap Linkage Disequilibrium images for ID4 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 600581

8 Novoseek disease relationships for ID4 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
lymphoblastic leukemia acute 34.21 3 12969807 (1), 17074115 (1), 18928580 (1)
leukemia 18.97 10 17074115 (3), 19379587 (2), 12969807 (1), 19099656 (1)
primary tumor 11.34 1 16867866 (1)
cancer 0.00 1 16106424 (1)
carcinoma 0.00 12 16867866 (7), 14633621 (4)
tumors 0.00 9 16867866 (2), 14633621 (2), 16018821 (2), 16541417 (1)
metastasis 0.00 1 16867866 (1)
prostate cancer 0.00 1 16106424 (1)
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(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/55 PubMed articles for ID4 gene (see all 55 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3400 HGNC: 5363 AceView: ID4 Ensembl:ENSG00000172201 euGenes: HUgn3400
ECgene: ID4 H-InvDB: ID4
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for ID4 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for ID4:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for ID4-related products

 Millipore Custom Antibody & Bulk Services
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 Predesigned and custom siRNAs for ID4 Antibodies for ID4
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for ID4 Browse small molecules at Sigma-Aldrich
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 shRNA in GFP-retroviral vector
 Search Tocris compounds for ID4




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for ID4

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