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ICT1 Gene

protein-coding GIFtS: 53

GC17P070520

immature colon carcinoma transcript 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
DS-1 ^¹, ², ³
DS1 ^³

Descriptions
Digestion substraction 1 ^³
immature colon carcinoma transcript 1 ^²

External Ids
HGNC: 5359¹
Entrez Gene: 3396²
UniProtKB: Q14197³
Ensembl: ENSG00000167862⁷

Search outside databases for aliases for ICT1 gene

Previous GC identifers: GC17M072980 GC17P076067 GC17P073472 GC17P073605

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ICT1:

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem

cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to

initiate in stem cells or their early descendants. One potential marker is ICT1 whose mRNA and

protein were more highly expressed in undifferentiated than in differentiated cells. [provided by

RefSeq]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the ICT1 gene

Entrez Gene cytogenetic band: 17q25.1 Ensembl cytogenetic band: 17q25.1 HGNC cytogenetic band: 17q25

ICT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17 GeneLoc Exon Structure

GeneLoc location for GC17P070520: (about GC identifiers)

Start:	70,520,371 bp from pter
End:	70,528,951 bp from pter
Size:	8,581 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000017.9 NT_010641.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ICT1_HUMAN, Q14197 (See protein sequence)

Recommended Name: Immature colon carcinoma transcript 1 protein precursor

Size: 206 amino acids; 23630 Da

Secondary accessions: Q53HM7 Q53Y11

REFSEQ proteins: NP_001536.1

ENSEMBL proteins:

ENSP00000301585

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (ICT1)
	Human Recombinant Proteins from Abnova (ICT1)

OriGene Purified Recombinant Human Protein: ICT1

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005575	cellular_component	ND	--

About this table

Antibodies for ICT1:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for ICT1
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (ICT1), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (ICT1)
	Novus Biologicals Antibodies for ICT1

Assays for ICT1:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q14197

ProtoNet protein and cluster: Q14197

UniProtKB/Swiss-Prot: ICT1_HUMAN, Q14197

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ICT1)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (ICT1)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001545

Applied Biosystems Silencer® siRNAs for ICT1

Sigma-Aldrich siRNA for ICT1

Sigma-Aldrich shRNA for ICT1

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for ICT1
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_001545
Myc/DDK tagged cDNA clone in CMV expression vector: NM_001545
untagged cDNA clones in CMV expression vector (see all 2): NM_001545

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_001545

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--
GO:0003747	translation release factor activity	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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3 Interacting proteins for ICT1 (ENSP00000301585³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ADSL	ENSP00000216194³	STRING (score=.816)
MMP11	ENSP00000215743³	STRING (score=.748)
SH2D3A	ENSP00000245908³	STRING (score=.74)

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2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006415	translational termination	IEA	--
GO:0008150	biological_process	ND	--

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ICT1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ICT1)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (ICT1)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001545

Sigma-Aldrich siRNA for ICT1

Sigma-Aldrich shRNA for ICT1

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_001545

REFSEQ mRNAs for ICT1 gene:

NM_001545.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_001545

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001545
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001545
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001545

Additional cDNA sequence:

AK222553.1 AK314138.1 BC015335.1 BT007111.1 X81788.1

4 DOTS entries:

DT.312338 DT.97843678 DT.97843679 DT.95287871

24/94 AceView cDNA sequences (see all 94 ):

AI688512 AA988569 BM744821 BM781675 BG178129 BM790637 BU161221 BM850252

BM773354 BM821741 T49953 BF724521 AA642525 BM785123 BM726088 AI692751

CB142973 CA337908 BC015335 BM764915 BM831332 AA524079 BM831439 BM832348

highest scoring ESTs for ICT1:

X81788 AA169383 AA207203 AA524079 AA575831 AA642525 AA665379 AI014780 AI349224 AI494072

Unigene Cluster for ICT1:

Immature colon carcinoma transcript 1

Hs.407955 [show with all ESTs]

Unigene Representative Sequence: BG177089

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for ICT1

ExUns:	1	^	2	^	3	^	4	^	5a	·	5b	·	5c	·	5d	^	6
SP1:			-						-
SP2:									-
SP3:			-

About this scheme

ECgene alternative splicing isoforms for ICT1

1 Ensembl transcript including schematic representation:

ENST00000301585

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ICT1 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for ICT1

¹ / ² / ³

3 probe-sets matching ICT1 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
40758_at²^{, 3}	U95-A	1	1.00	1.00	1.00	1.00	X81788	1.00	1.00	1.00	1
204868_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_001545	0.60	1.00	0.82	1
204868_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks ICT1 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: TATTAGTCAC

SOURCE GeneReport for Unigene cluster: Hs.407955
Expression variation in blood from EXPOLDB for ICT1
UniProtKB/Swiss-Prot: ICT1_HUMAN, Q14197
Tissue specificity: Down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma
cells

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for ICT1 gene from 5/14 species (see all 14 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	ICT1¹	--	immature colon carcinoma transcript 1	99.19(n) 99.03(a)	736364 XM_001138419.1 XP_001138419.1
cow (Bos taurus)	ICT1¹	--	immature colon carcinoma transcript 1	83.17(n) 80.1(a)	515465 NM_001034493.1 NP_001029665.1
rat (Rattus norvegicus)	Ict1¹	--	immature colon carcinoma transcript 1	81.72(n) 80.1(a)	303673 XM_221110.4 XP_221110.3
mouse (Mus musculus)	Ict1^{1, 5}	11⁵	immature colon carcinoma transcript 1^{1, 5}	81.73(n)¹ 79.66(a)¹	68572¹ NM_026729.1¹ NP_081005.1¹ AI844357⁵ AK003192⁵ (see all 13)
chicken (Gallus gallus)	ICT1¹	--	immature colon carcinoma transcript 1	63.35(n) 62.3(a)	422114 XM_420117.2 XP_420117.2

About this table Species with no ortholog for ICT1

ENSEMBL Gene Tree for ICT1

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/81 NCBI SNPs in ICT1 are shown (see all 81 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 42)

AB	SNP ID	Valid	Chr 17 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs11077771^1,2	C,F,H	70519449(+)	`CACTGC/ATTGCT`	1	--	ng5¹		4	EU EA WA	420
	rs9907177^1,2	C,F,H	70518591(+)	`CATCTC/TGGCCT`	1	--	ng5¹		4	EU EA WA	420
	rs10512599^1,2	C,F,H	70527397(-)	`ATTGTC/GAAGCG`	1	L/F	mis¹		17	NS EU EA WA NA	2486
--	rs3744206^1,2	C,F	70520399(+)	`CCTGCC/G/TCTGGG`	2	R/P R/L	mis¹ ese³		4	EA MN NA	1716
	rs16967525^1,2	F,H	70528953(+)	`CTCATT/CAGGCT`	1	--	ng3¹		7	NA EU EA WA	562
	rs8068910^1,2	H	70528319(+)	`CAACAA/TAAGAA`	1	K/X	non¹ ese³		4	EU EA WA	418
	rs1044228^1,2	C,F,H,O	70528216(+)	`ATCCTC/TACCTC`	1	L/L	syn¹ ese³		16	EA MN NA EU WA	4188
	rs902726^1,2	A,C,F,H,O	70527851(-)	`AAGCCC/TGCAAA`	1	--	int¹		10	EU EA WA NA	3526
--	rs7217394^1,2	A,C,F,H	70522107(+)	`tgatcA/Gcgggc`	1	--	int¹		4	EU EA WA	420
	rs9890550^1,2	A,C,F,H	70526535(+)	`CAAAGA/GTGCTG`	1	--	int¹		8	EU EA WA	838

About this table

HapMap Linkage Disequilibrium images for ICT1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 603000

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

8 PubMed articles for ICT1 gene:

Identification of mRNAs that show modulated expression during colon carcinoma cell differentiation. (PubMed id 8575443)^{1, 2, 3, 4} van Belzen N.... Bosman F.T. (1995)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross- validation of MS/MS and MS/MS/MS Spectra. (PubMed id 17924679)⁴ Yu L.-R.... Veenstra T.D. (2007)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)³ Suzuki Y.... Sugano S. (1997)
A novel T-cell differentiation antigen expressed in immature human thymocytes and neuroglial cells. (PubMed id 7545548)³ Bae Y.M....Ahn H.S. (1995)
Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)³ Maruyama K. and Sugano S. (1994)
Expression of differentiation-related genes in colorectal cancer: possible implications for prognosis. (PubMed id 9810514)¹ van Belzen N....Bosman F.T. (1998)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 3396	HGNC: 5359	AceView: ICT1	Ensembl:ENSG00000167862	euGenes: HUgn3396
ECgene: ICT1	H-InvDB: ICT1

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
ATLAS Chromosomes in Cancer entry for ICT1	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for ICT1
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for ICT1:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for ICT1-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for ICT1		Antibodies for ICT1
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for ICT1		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (ICT1)
	Antibodies (ICT1)
	Chimera RNAi (ICT1)
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

Search for Antibodies & Assays

	Recombinant Proteins (ICT1)
	Antibodies (ICT1)

	Tagged/untagged cDNA clones
	Validated SYBR primer pairs
	Purified proteins from HEK293T cells
	Western blot validated antibodies
	shRNA in GFP-retroviral vector

Search Tocris compounds for ICT1

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for ICT1

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ICT1 Gene at Home site.
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ICT1 Gene

protein-coding GIFtS: 53

Aliases &
Descriptions
for ICT1

Summaries
for ICT1

Genomic Location
for ICT1

Proteins
for ICT1

Protein Domains/
Families
for ICT1

Gene Function
for ICT1

Pathways & Interactions
for ICT1

Drugs & Compounds
for ICT1

Transcripts
for ICT1

Expression
for ICT1

Orthologs
for ICT1

Paralogs
for ICT1

SNPs/Variants
for ICT1

Disorders & Mutations
for ICT1

Medical News
for ICT1

Publications
for ICT1

Search for ICT1

Genome Databases showing ICT1

Other Databases showing ICT1

Specialized Databases showing ICT1

Licensable Technologies
for ICT1

Services
for ICT1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

ICT1 Gene

protein-coding GIFtS: 53

Aliases & Descriptionsfor ICT1

Summariesfor ICT1

Genomic Locationfor ICT1

Proteinsfor ICT1

Protein Domains/Familiesfor ICT1

Gene Functionfor ICT1

Pathways & Interactionsfor ICT1

Drugs & Compoundsfor ICT1

Transcriptsfor ICT1

Expression for ICT1

Orthologsfor ICT1

Paralogsfor ICT1

SNPs/Variantsfor ICT1

Disorders & Mutationsfor ICT1

Medical Newsfor ICT1

Publicationsfor ICT1

Search for ICT1

Genome Databases showing ICT1

Other Databases showing ICT1

Specialized Databases showing ICT1

Licensable Technologiesfor ICT1

Servicesfor ICT1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for ICT1

Summaries
for ICT1

Genomic Location
for ICT1

Proteins
for ICT1

Protein Domains/
Families
for ICT1

Gene Function
for ICT1

Pathways & Interactions
for ICT1

Drugs & Compounds
for ICT1

Transcripts
for ICT1

Expression
for ICT1

Orthologs
for ICT1

Paralogs
for ICT1

SNPs/Variants
for ICT1

Disorders & Mutations
for ICT1

Medical News
for ICT1

Publications
for ICT1

Licensable Technologies
for ICT1

Services
for ICT1