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IARS2 Gene

protein-coding   GIFtS: 53

GC01P218334
isoleucyl-tRNA synthetase 2, mitochondrial
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.1.1.5 3
FLJ10326 1, 2
ILERS 2
IleRS 3
Descriptions
Isoleucine--tRNA ligase 3
isoleucine tRNA ligase 2, mitochondrial 2
isoleucine-tRNA synthetase 2, mitochondrial 2
isoleucyl-tRNA synthetase 2, mitochondrial 2
mitochondrial isoleucine tRNA synthetase 2
External Ids
HGNC: 296851
Entrez Gene: 556992
UniProtKB: Q9NSE43
Ensembl: ENSG000000677047
Search outside databases for aliases for IARS2 gene

Previous GC identifer: GC01P216656

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the IARS2 gene  

Entrez Gene cytogenetic band: 1q41   Ensembl cytogenetic band:  1q41   HGNC cytogenetic band: 1q41

IARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P218334:     (about GC identifiers)

Start:
218,334,067 bp from pter
End:
218,388,006 bp from pter
Size:
53,940 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_021877.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYIM_HUMAN, Q9NSE4 (See protein sequence)
Recommended Name: Isoleucyl-tRNA synthetase, mitochondrial precursor  
Size: 1012 amino acids; 113792 Da
Subcellular location: Mitochondrion matrix (By similarity)
Sequence caution: Sequence=BAA91544.1; Type=Frameshift; Positions=879;
Secondary accessions: Q1M2P9 Q6PI85 Q7L439 Q86WU9 Q96D91 Q9H9Q8 Q9NW42

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_060530.3  

    ENSEMBL proteins: 
    ENSP00000303279 ENSP00000355889 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (IARS2)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0005739 mitochondrion IEA--
    GO:0005759 mitochondrial matrix IEA--
    About this table

    Antibodies for IARS2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for IARS2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (IARS2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (IARS2)
    Search Novus for antibodies for IARS2

    Assays for IARS2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR001412 aa-tRNA-synth_I_CS
     IPR018353 Isoleucyl-tRNA_synthetase
     IPR002301 Ile-tRNA-synt_Ia
     IPR015905 Ile-tRNA-synt_Ia_N
     IPR014729 Rossmann-like_a/b/a_fold


       GeneDecks  IARS2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NSE4

    ProtoNet protein and cluster: Q9NSE4

    2 Blocks protein families:
    IPB002300 Aminoacyl-tRNA synthetase
    IPB002301 Isoleucyl-tRNA synthetase signature


    UniProtKB/Swiss-Prot: SYIM_HUMAN, Q9NSE4
    Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (IARS2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (IARS2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018060

                  Applied Biosystems Silencer® siRNAs for IARS2

                  Sigma-Aldrich siRNA for IARS2  
                         Sigma-Aldrich shRNA for IARS2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018060
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018060
                                     untagged cDNA clones in CMV expression vector (see all 2): D28500 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018060

    UniProtKB/Swiss-Prot: SYIM_HUMAN, Q9NSE4
    Catalytic activity: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
    Enzyme Number (IUBMB): EC 6.1.1.5 

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0004822 isoleucine-tRNA ligase activity IEA--
    GO:0005524 ATP binding IEA--
    GO:0016874 ligase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  IARS2  (Your Favorite Gene powered by Ingenuity) 
     Aminoacyl-tRNA Biosynthesis
     Valine, Leucine and Isoleucine Biosynthesis

       GeneDecks  IARS2 for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for IARS2):
     hsa00290 Valine, leucine and isoleucine biosynthesis
     hsa00970 Aminoacyl-tRNA biosynthesis

       GeneDecks  IARS2 for the pathways selected above  
    About GeneDecksing

    4 Interacting proteins for IARS2 (ENSP000003032793) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    BCAT1ENSP000002611923STRING (score=.899)
    BCAT2ENSP000003229913STRING (score=.899)
    IARSENSP000003648113STRING (score=.828)
    QRSL1ENSP000003580423STRING (score=.704)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006428 isoleucyl-tRNA aminoacylation IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for IARS2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (IARS2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (IARS2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018060

                  Sigma-Aldrich siRNA for IARS2  
                         Sigma-Aldrich shRNA for IARS2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018060  

    REFSEQ mRNAs for IARS2 gene: 

    NM_018060.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018060  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018060
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018060
                                     untagged cDNA clones in CMV expression vector (see all 2): D28500 

    Additional cDNA sequence: 

    AK001188.1 AK001726.1 AK022665.1 AK291432.1 AY267462.1 AY952883.1 BC010218.1 BC040376.1 
    BC047880.1 BC137438.1 CR606160.1 CR621413.1 CR622315.1 D28500.1 

    17 DOTS entries:

    DT.99934757  DT.121336558  DT.95214751  DT.453718  DT.92444800  DT.121336545  DT.75150403  DT.95214749 
    DT.97854650  DT.100822365  DT.121336583  DT.121336617  DT.92444799  DT.95214813  DT.95214815  DT.99966773 
    DT.91910585 

    24/291 AceView cDNA sequences (see all 291 ):

    BF981431 BM827798 AI636307 AK022665 AA962169 AI469501 AA437174 BG283854 
    AI915924 AU118659 AW268711 AK001726 AU132576 BE220678 AW188441 AA805660 
    BG620018 AU132991 BE440113 CR622315 CB306960 AI863816 AI220196 AW167114 

    highest scoring ESTs for IARS2:

    AA526812 AA042812 AA094477 AA232722 AA236516 AA426442 AA523339 AA523446 AA523691 AA548731 

    Unigene Cluster for IARS2:

    Isoleucyl-tRNA synthetase 2, mitochondrial
    Hs.262823  [show with all ESTs]
    Unigene Representative Sequence: AY267462


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000302637  ENST00000366922  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    IARS2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for IARS2

    1 / 2 / 3

    4 probe-sets matching IARS2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    36647_at2, 3 U95-A 1 1.00 1.00 0.75 1.49 AA526812 1.00 1.00 1.00 1

    63913_at2, 3 U95-C 1 0.31 1.00 0.91 0.54 AI681541 0.40 1.00 0.76 1

    217900_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018060 0.60 1.00 0.82 1

    217900_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  IARS2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CAGAAATATA

    SOURCE GeneReport for Unigene cluster: Hs.262823

    Expression variation in blood from EXPOLDB for IARS2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for IARS2 gene from 5/20 species (see all 20 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    IARS21   -- isoleucyl-tRNA synthetase 2, mitochondrial 89.61(n)
    90.82(a)
    478963  XM_536121.2  XP_536121.2 
    chimpanzee
    (Pan troglodytes)
    IARS21   -- isoleucyl-tRNA synthetase 2, mitochondrial 99.31(n)
    99.6(a)
    457749  XM_514209.2  XP_514209.2 
    cow
    (Bos taurus)
    IARS21   -- isoleucyl-tRNA synthetase 2, mitochondrial 88.79(n)
    89.35(a)
    533057  XM_612328.3  XP_612328.3 
    rat
    (Rattus norvegicus)
    Iars21   -- isoleucyl-tRNA synthetase 2, mitochondrial 74.69(n)
    74.33(a)
    364070  XM_344185.3  XP_344186.3 
    mouse
    (Mus musculus)
    Iars21, 5 15
    isoleucine-tRNA synthetase 2, mitochondrial1, 5 81.59(n)1
    83.4(a)1
    3813141  NM_198653.21  NP_941055.11 
     AK0080425  AK0361825  (see all 23)
    About this table        Species with no ortholog for IARS2

    ENSEMBL Gene Tree for IARS2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for IARS2 gene
    VARS2  IARS2  VARS22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/165 NCBI SNPs in IARS2 are shown (see all 165 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 52)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs110951,2
    A,C,F,H218387646(+) TGGCTA/GGAAGT 1 -- ut31 ese311Minor allele frequency- G:0.21MN EA EU WA NA 2634
    rs118003051,2
    C,F,H218354363(+) GGTGTA/GTATCA 1 I/V mis1 ese38Minor allele frequency- G:0.02EU EA WA 840
    --
    rs25771541,2
    A,C218334222(-) CCAGGA/GCGGCC 1 A/V mis1 ese30--------
    rs66815161,2
    H218345876(+) ATGGTA/TCTTGC 1 T/S mis14Minor allele frequency- T:0.00EU EA WA 418
    --
    rs75433171,2
    A218387957(+) TTGACC/TCTGTA 1 -- ut31 ese30--------
    rs14658161,2
    A,C,F,H,O218356426(+) AATTTC/TTTTTA 1 -- int16Minor allele frequency- G:0.06EA NA 390
    --
    rs27897881,2
    A,C,F,H,O218342001(+) TGTCTG/ATAGCA 1 -- int17Minor allele frequency- A:0.47NA EU EA WA 562
    rs27897891,2
    A,C,F,H,O218342956(+) GTTACG/AAAGTG 1 -- int17Minor allele frequency- A:0.47EU EA WA NA 558
    rs28080221,2
    A,C,F,H218368740(+) TTTCAG/ATTTCT 1 -- int112Minor allele frequency- A:0.37EU EA WA 1254
    rs10392061,2
    C,F,H,O218355055(-) AAAGAC/TTCAGA 1 -- int113Minor allele frequency- N:0.01MN EA NA EU WA 1236
    About this table

    HapMap Linkage Disequilibrium images for IARS2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 612801

    Human Genome Epidemiology Navigator: IARS2 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for IARS2 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55699 HGNC: 29685 AceView: FLJ10326 Ensembl:ENSG00000067704 euGenes: HUgn55699
    ECgene: IARS2 H-InvDB: IARS2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for IARS2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for IARS2 Antibodies for IARS2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for IARS2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search Novus for antibodies for IARS2

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