Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

HUS1 Gene

protein-coding   GIFtS: 60
GCID: GC07M047735

HUS1 checkpoint homolog (S. pombe)

(Previous name: HUS1 (S. pombe) checkpoint homolog )
 Explore 11 diseases affiliated with
HUS1 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for HUS1    

Aliases
for HUS1 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
HUS1 Checkpoint Homolog (S. Pombe)1 2
HHUS11
HUS1 (S. Pombe) Checkpoint Homolog1
Checkpoint Protein HUS12
Hus1+-Like Protein2

External Ids:    HGNC: 53091   Entrez Gene: 33642   Ensembl: ENSG000001362737   OMIM: 6037605   UniProtKB: O609213   

Export aliases for HUS1 gene to outside databases

Previous GC identifers: GC07M047650 GC07M047744 GC07M047777 GC07M047969


Summaries
for HUS1 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for HUS1:
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex
that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with
checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein
complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads
the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the
activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative
splicing results in multiple transcript variants. (provided by RefSeq, Feb 2011)

UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921
Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The
9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex.
Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair
(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH
end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage
activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on
long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of
double-stranded breaks (DSB) occurring during the S phase

Gene Wiki entry for HUS1


Genomic Views
for HUS1 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000007.13  NC_018918.1  NT_007819.17  NT_079592.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the HUS1 gene promoter:
         Max1   Spz1   C/EBPbeta   AML1a   POU6F1 (c2)   STAT5A   Evi-1   FAC1   RSRFC4   c-Myc   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): HUS1 promoter sequence
   Search SABiosciences Chromatin IP Primers for HUS1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HUS1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7p13-p12   Ensembl cytogenetic band:  7p12.3   HGNC cytogenetic band: 7p13-p12

HUS1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
HUS1 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M047735:  view genomic region     (about GC identifiers)

Start:
 47,735,328 bp from pter      End:
 48,019,246 bp from pter
Size:
 283,919 bases      Orientation:
 minus strand

1 alternative location:
Chr7-,CRA_TCAG 48,043,728-48,059,198     

Proteins
for HUS1 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921 (See protein sequence)
Recommended Name: Checkpoint protein HUS1  
Size: 280 amino acids; 31691 Da
Subunit: Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex
associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC
complex. HUS1 interacts with POLB, HDAC1, FEN1, PCNA, RAD1, RAD9A and RAD9B. HUS1 does not interact with RAD17.
Interacts with DNAJC7
Subcellular location: Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According to PubMed:14500360,
localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the
cytoplasm
3 PDB 3D structures from and Proteopedia for HUS1:
3A1J (3D)        3G65 (3D)        3GGR (3D)    

Explore the universe of human proteins at neXtProt for HUS1: NX_O60921

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O60921

    • HUS1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_004498.1  
    ENSEMBL proteins: 
     ENSP00000403844   ENSP00000258774   ENSP00000400727   ENSP00000416588   ENSP00000404348  
     ENSP00000404855   ENSP00000405175   ENSP00000398806  
    Reactome Protein details: O60921
    Human Recombinant Protein Products: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for HUS1
    OriGene Purified Protein: HUS1
    OriGene Protein Over-expression Lysate: HUS1
    OriGene Custom Protein Services for HUS1 
    GenScript Custom Purified and Recombinant Proteins Services for HUS1
    Novus Biologicals HUS1 Proteins
    Novus Biologicals HUS1 Lysates
    Browse Sino Biological Recombinant Proteins
    ProSpec Recombinant Protein for HUS1
    Uscn Proteins for HUS1

    Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA10846170
    GO:0005654nucleoplasm TAS--
    GO:0005730nucleolus IDA--
    GO:0005794Golgi apparatus IDA--
    GO:0030896checkpoint clamp complex IEA--


    HUS1 for ontologies           About GeneDecksing



    HUS1 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies (see all 2): HUS1
    OriGene Custom Antibody Services for HUS1 
    GenScript Custom Superior Antibodies Services for HUS1
    Novus Biologicals HUS1 Antibodies
    Abcam antibodies for HUS1 
    Uscn Antibodies for HUS1
    ThermoFisher Antibodies for HUS1

    Assay Products for HUS1: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Immunoassay Development
    Browse OriGene Fluorogenic Cell Assay Kits
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for HUS1
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for HUS1

    Protein Domains /
    Families
    for HUS1 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HUS1 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR007150 Hus1/Mec3
     IPR016580 Cell_cycle_HUS1

    Graphical View of Domain Structure for InterPro Entry O60921

    ProtoNet protein and cluster: O60921

    UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921
    Similarity: Belongs to the HUS1 family


    Function
    for HUS1 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921
    Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The
    9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex.
    Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair
    (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH
    end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage
    activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on
    long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of
    double-stranded breaks (DSB) occurring during the S phase

         Genatlas biochemistry entry for HUS1:
    cell cycle G2 checkpoint control gene,homologous to S pombe Hos1

    miRNA
    Products:            		 OriGene 3'-UTR Clone: HUS1
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat HUS1
    2 QIAGEN miScript miRNA Assays for microRNAs that regulate HUS1:
    hsa-miR-200c* hsa-miR-550a*
    SwitchGear 3'UTR luciferase reporter plasmid: HUS1 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HUS1 (see all 7)
    OriGene shRNA RFP: HUS1
    OriGene siRNA: HUS1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HUS1

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for HUS1

    Clone
    Products:             		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HUS1 (see all 4)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HUS1
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: HUS1 (NM_004507)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for HUS1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HUS1 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for HUS1
    Search LifeMap BioReagents cell lines for HUS1

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HUS1

    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI19464297


    HUS1 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-outs for HUS1: Hus1tm1Led Hus1tm1.1Rsw
         9 MGI mutant phenotypes (inferred from 4 alleles) (MGI details for Hus1):
     cardiovascular system  cellular  embryogenesis  growth/size  hematopoietic system 
     limbs/digits/tail  mortality/aging  nervous system  tumorigenesis 

    HUS1 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for HUS1 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/7 super-pathways (see all 7) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Cell cycle_Role of 14-3-3 proteins in cell cycle regulation
    		Cell cycle_Role of 14-3-3 proteins in cell cycle regulation1.00
    		Cell cycle Role of 14-3-3 proteins in cell cycle regulation1.00
    2Activation of ATR in response to replication stress
    		Activation of ATR in response to replication stress1.00
    		G2/M Checkpoints0.86
    3Chks in Checkpoint Regulation
    		Chks in Checkpoint Regulation1.00
    		DNA Repair Mechanisms0.32
    4Cell Cycle
    		Cell Cycle1.00
    5Regulation of Telomerase
    		Regulation of Telomerase1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    1 EMD Millipore Pathway for HUS1
        Cell cycle Role of 14-3-3 proteins in cell cycle regulation

    3 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for HUS1
        DNA Repair Mechanisms
    Chks in Checkpoint Regulation
    DNA Damage Induced 14-3-3Sigma Signaling

    1 GeneGo (Thomson Reuters) Pathway for HUS1
        Cell cycle Role of 14-3-3 proteins in cell cycle regulation

    1 BioSystems Pathway for HUS1 
        Regulation of Telomerase

    4        Reactome Pathways for HUS1
        Activation of ATR in response to replication stress
    Cell Cycle
    G2/M Checkpoints
    Cell Cycle Checkpoints



    HUS1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for HUS1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/59 Interacting proteins for HUS1 (O609211, 2, 3 ENSP000002587744) via UniProtKB, MINT, STRING, and/or I2D (see all 59)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RAD9AQ996381, 3, ENSP000003113604EBI-1056174,EBI-2606224 I2D: score=4 STRING: ENSP00000311360
    RAD1O606711, 3, ENSP000003408794EBI-1056174,EBI-721835 I2D: score=3 STRING: ENSP00000340879
    YWHAZP631042, 3, ENSP000003095034MINT-3318251 I2D: score=1 STRING: ENSP00000309503
    PCNAP120043, ENSP000003684384I2D: score=2 STRING: ENSP00000368438
    ALDOBP050623, ENSP000003639884I2D: score=1 STRING: ENSP00000363988
    About this table

    Gene Ontology (GO): 5/13 biological process terms (GO ID links to tree view) (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000075cell cycle checkpoint TAS--
    GO:0000077DNA damage checkpoint IMP--
    GO:0000724double-strand break repair via homologous recombination IEA--
    GO:0001932regulation of protein phosphorylation IEA--
    GO:0006260DNA replication TAS--


    HUS1 for ontologies           About GeneDecksing



    Drugs & Compounds
    for HUS1 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    HUS1 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for HUS1
    1 Novoseek chemical compound relationship for HUS1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    hydroxyurea 47.7 3 11077446 (1), 17327271 (1)

    Search CenterWatch for drugs/clinical trials and news about HUS1 

    Transcripts
    for HUS1 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for HUS1 gene: 
    NM_004507.3  

    Unigene Cluster for HUS1:

    HUS1 checkpoint homolog (S. pombe)
    Hs.152983  [show with all ESTs]
    Unigene Representative Sequence: NM_004507
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000436444 ENST00000258774(uc003tod.2) ENST00000458191 ENST00000432325
    ENST00000442024 ENST00000432627 ENST00000433977 ENST00000446009 ENST00000468868


    miRNA
    Products:             		 OriGene 3'-UTR Clone: HUS1
    Browse OriGene MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat HUS1
    2 QIAGEN miScript miRNA Assays for microRNAs that regulate HUS1:
    hsa-miR-200c* hsa-miR-550a*
    SwitchGear 3'UTR luciferase reporter plasmid: HUS1 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HUS1 (see all 7)
    OriGene shRNA RFP: HUS1
    OriGene siRNA: HUS1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HUS1
    Clone
    Products:             		 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HUS1 (see all 4)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HUS1
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector: HUS1 (NM_004507)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for HUS1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HUS1 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HUS1
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat HUS1
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HUS1
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HUS1

    Additional cDNA sequence: 

    AJ227901.1 AK097182.1 AK290459.1 AK294117.1 BC007013.1 BT019481.1 BT019482.1 CR536552.1 
    NR_037917.1 Y16893.1 

    8 DOTS entries:

    DT.310408  DT.70103554  DT.92424430  DT.102823648  DT.92424429  DT.95259816  DT.91652334  DT.95362322 

    24/87 AceView cDNA sequences (see all 87):

    AW132130 R95460 BX500811 AL554895 CR619988 NM_004507 AA828114 BQ646867 
    CR536552 BF222727 AI538328 AA854956 CA418558 BU193379 AI167268 BC007013 
    AA897773 AI968159 AI675254 Y16893 BU171912 BF510091 BX115181 AA353895 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for HUS1    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10 ^ 11
    SP1:              -                 -           -           -                 -                     
    SP2:                                                                          -                     
    SP3:                                -                                                               
    SP4:              -                                                                                 
    SP5:                                                                                                


    ECgene alternative splicing isoforms for HUS1

    Expression
    for HUS1 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    HUS1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image
    See HUS1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for HUS1

    SOURCE GeneReport for Unigene cluster: Hs.152983

    UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921
    Tissue specificity: Ubiquitous

        SABiosciences Expression via Pathway-Focused PCR Arrays including HUS1: 
              Cell Cycle in human mouse rat
              DNA Damage Signaling Pathway in human mouse rat
              Stress & Toxicity PathwayFinder in human mouse rat

    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HUS1
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat HUS1
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HUS1
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HUS1
    In Situ
    Assay Products:     
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HUS1

    Orthologs
    for HUS1 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of animals.

    Orthologs for HUS1 gene from 6/24 species (see all 24)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    chicken
    (Gallus gallus)    		 Aves HUS11 HUS1 checkpoint homolog (S. pombe) 74.64(n)
    76.07(a)    		   428471  XM_426029.3  XP_426029.3 
    lizard
    (Anolis carolinensis)    		 Reptilia --
    		 --
    		 72(a)
    		 1 → many
    		 6(53964778-53984555)
    African clawed frog
    (Xenopus laevis)    		 Amphibia LOC3985712 Hus1 protein 76.55(n)    AY169966.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii zgc:1628951 zgc:162895 65.35(n)
    65.95(a)    		   100037341  NM_001089496.1  NP_001082965.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta Hus1-like1 CG2525-PA 43.79(n)
    31.73(a)    		   40598  NM_058078.3  NP_477426.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea hus-11 Protein HUS-1 43.28(n)
    32.46(a)    		   171939  NM_058802.5  NP_491203.1 


    ENSEMBL Gene Tree for HUS1 (if available)
    TreeFam Gene Tree for HUS1 (if available) 

    Paralogs
    for HUS1 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for HUS1 gene
    HUS1B2  
    1 SIMAP similar gene for HUS1 using alignment to 7 protein entries:     HUS1_HUMAN (see all proteins):
    HUS1B

    HUS1 for paralogs           About GeneDecksing



    Genomic Variants
    for HUS1 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/394 NCBI SNPs in HUS1 are shown (see all 394)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 7 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1818277331,2
    		--48002393(+) AATTGC/TCTGGG 2 -- int10--------
    rs1852270521,2
    		--48002454(+) TGTGCC/GTACCA 2 -- int10--------
    rs1896988321,2
    		--48002661(+) CAAATC/TATATT 2 -- int10--------
    rs1392118151,2
    		--48002704(+) TGACCC/TGGCAA 2 -- int10--------
    rs1806823661,2
    		--48002729(+) AGATCA/TTTCAA 2 -- int10--------
    rs31766211,2
    		C,--48002895(-) CTTAGT/ATGAGA 2 -- nc-transcript-variantut311Minor allele frequency- A:0.01NS 170
    rs1865807041,2
    		--48002970(+) ATATGC/TATTTG 2 -- nc-transcript-variantut310--------
    rs31766201,2
    		C,--48003065(-) TGAAACA/-ATATC 2 -- ut31 nc-transcript-variant1Minor allele frequency- -:0.01NS 180
    rs31766191,2
    		C,F,H--48003197(-) GATGGA/GATATT 2 -- nc-transcript-variantut316Minor allele frequency- G:0.07NS EA 1024
    rs31766181,2
    		F--48003214(-) TAATAA/GTGTTG 2 -- ut31 nc-transcript-variant1Minor allele frequency- G:0.01NS 174

    HapMap Linkage Disequilibrium report for HUS1 (47735328 - 47985328 bp, first 250kb of HUS1)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for HUS1: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HUS1
    DNA2.0 Custom Variant and Variant Library Synthesis for HUS1

    Disorders / Diseases
    for HUS1 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    HUS1 for disorders           About GeneDecksing

    OMIM gene information: 603760    OMIM disorders: --

    11 diseases for HUS1:    About MalaCards
    amyotrophic lateral sclerosis    lateral sclerosis    parathyroid adenoma    sporadic breast cancer
    breast cancer    pulmonary disease    lung carcinoma    adenoma
    esophagitis    adenocarcinoma    carcinoma

    2 Novoseek disease relationships for HUS1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 0 2 18156970 (2)
    cancer 0 1 18156970 (1)

    Human Genome Epidemiology (HuGE) Navigator: HUS1 (9 documents)

    Export disorders for HUS1 gene to outside databases

    Publications
    for HUS1 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for HUS1 gene, integrated from 9 sources (see all 76):
    (articles sorted by number of sources associating them with HUS1)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster. (PubMed id 9878245)1, 2, 3, 9 Dean F.B.... O'Donnell M. (1998)
    2. Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage. (PubMed id 9524127)1, 2, 3 Kostrub C.F.... Enoch T. (1998)
    3. The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9. (PubMed id 10884395)1, 2, 9 Rauen M.... Karnitz L.M. (2000)
    4. HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. (PubMed id 10846170)1, 2, 9 Cai R.L.... Cohen D. (2000)
    5. PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. (PubMed id 11077446)1, 2, 9 Komatsu K.... Wang H.-G. (2000)
    6. The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1. (PubMed id 10359610)1, 2, 9 St Onge R.P.... Davey S. (1999)
    7. The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9. (PubMed id 11573955)1, 2, 9 Xiang S.L....Yamamoto K.C. (2001)
    8. Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. (PubMed id 15897895)1, 2, 9 Wu X.... Zou Y. (2005)
    9. The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair. (PubMed id 15314187)1, 2, 9 Toueille M....Huebscher U. (2004)
    10. The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery. (PubMed id 15871698)1, 2 Smirnova E.... Huebscher U. (2005)

    External Searches for HUS1 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing HUS1 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 3364 HGNC: 5309 AceView: HUS1 Ensembl:ENSG00000136273 euGenes: HUgn3364
    ECgene: HUS1 H-InvDB: HUS1

    Other Databases showing HUS1 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing HUS1 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for HUS1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for HUS1 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/hus1/

    Intellectual Property
    for HUS1 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for HUS1 gene:
    Search GeneIP for patents involving HUS1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
    for HUS1 gene

    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Phosphatase Activity Assays/Reagents   Browse Recombinant/Natural Proteins  
     OriGene Antibodies for HUS1   OriGene shRNA RFP for HUS1  
     OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HUS1   OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HUS1  
     OriGene Protein Over-expression Lysate for HUS1   Browse OriGene Fluorogenic Cell Assay Kits  
     OriGene siRNA for HUS1   OriGene 3'-UTR Clone for HUS1  
     OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HUS1   OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HUS1  
     Browse OriGene GFP tagged cDNA clones in CMV expression vector   Browse OriGene MicroRNA Expression Plasmids  
     Browse OriGene basic RS shRNAs   Browse OriGene validated miRNA SYBR primer pairs  
     OriGene Purified Protein for HUS1   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for HUS1   OriGene Custom Protein Services for HUS1  
     OriGene Custom Immunoassay Development  

         		 
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat HUS1
     QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HUS1
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HUS1
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HUS1
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HUS1
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HUS1
     GenScript Custom Purified and Recombinant Proteins Services for HUS1 GenScript cDNA clones with any tag delivered in your preferred vector for HUS1
     GenScript Custom Assay Services for HUS1 GenScript Custom Superior Antibodies Services for HUS1
     GenScript Custom overexpressing Cell Line Services for HUS1 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in HUS1 promoter
     Search Chromatin IP Primers for HUS1
     RT2 qPCR Primer Assay in human, mouse, rat HUS1
     GNC Network for HUS1
     SABiosciences PCR Arrays including human, mouse, rat HUS1
     Search Tocris compounds for HUS1
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological cDNA Clones
     Antibodies/Proteins Production Services
     Rabbit Monoclonal Antibody Platform
     Bulk Purchasing
     Proteins for HUS1
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     HUS1 antibodies
     HUS1 proteins
     HUS1 lysates
     Antibodies for HUS1
     See all of Abcam's Antibodies, Kits and Proteins for HUS1
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Recombinant Protein for HUS1




     HUS1 Proteins, Antibodies, CLIAs, and ELISAs
     Search LifeMap BioReagents cell lines for HUS1
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HUS1
     SwitchGear 3'UTR luciferase reporter plasmids for HUS1
     SwitchGear Promoter luciferase reporter plasmids for HUS1
     ThermoFisher Antibodies for HUS1
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HUS1
           
    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

    View Random Gene

    Category
    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 

    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Copyright © 1996-2013 , Weizmann Institute of Science. All Rights Reserved.
    Hot genes      Disease genes      HUS1 gene at Home site.
    hostname: 356977-web1.xennexinc.com index build: 100 solr: 1.4