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Aliases &
Descriptions
for HUS1
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| | | Descriptions |
|---|
| HUS1 (S. pombe) checkpoint homolog 1 | | HUS1 checkpoint homolog (S. pombe) 2 | | HUS1 checkpoint protein 2 | | hus1+-like protein 2 |
|
| |
Search outside databases for aliases for HUS1 genePrevious GC identifers: GC07M047650 GC07M047744 GC07M047777 |
Summaries
for HUS1(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for HUS1:
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated
checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This
protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA
damage, the trimeric complex interacts with another protein complex consisting of checkpoint
protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined
complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on
the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling
event. [provided by RefSeq]
UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in
DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication
factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several
proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA
polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the
primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1
cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths;
and DNA ligase I (LIG1) on long-patch base excision repair substratesGene Wiki entry for HUS1 |
Genomic Location
for HUS1
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences)
About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
 Transcription factor binding sites upstream to the HUS1 gene 
Entrez Gene cytogenetic band: 7p13-p12 Ensembl cytogenetic band: 7p12.3 HGNC cytogenetic band: 7p13-p12HUS1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07M047969:
(about GC identifiers)
Start:
|
47,970,308 bp from pter |
End:
|
47,985,771 bp from pter |
Size:
|
15,464 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr7-,CRA_TCAG 48,043,728-48,059,198 |
RefSeq DNA sequence:- NC_000007.12 NT_007819.16 NT_079592.2
| Proteins
for HUS1
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921 (See
protein sequence)Recommended Name: Checkpoint protein HUS1 Size: 280 amino acids; 31691 Da
Subunit: Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and
HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1
complex associates with the RAD17-RFC complex. HUS1 interacts with POLB, HDAC1, FEN1, PCNA, RAD1,
RAD9A and RAD9B. HUS1 does not interact with RAD17
Subcellular location: Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According
to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation.
Shuttles between the nucleus and the cytoplasmREFSEQ proteins: NP_004498.1
ENSEMBL proteins: ENSP00000258774
Human Recombinant Proteins  Browse Origene for full length recombinant human proteins expressed in human HEK293 cells
2 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for HUS1:
Assays for HUS1: | Protein
Domains/
Families
for HUS1(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry O60921
ProtoNet protein and cluster: O60921 UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921Similarity: Belongs to the HUS1 family | Gene Function
for HUS1
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004507
 Applied Biosystems Silencer® siRNAs for HUS1
 Sigma-Aldrich siRNA for HUS1
Sigma-Aldrich shRNA Panels and shRNA for HUS1
Explore Sigma-Aldrich super-pooled esiRNAs
OriGene GFP tagged cDNA clone in CMV expression vector: NM_004507
Myc/DDK tagged cDNA clone in CMV expression vector: NM_004507
untagged cDNA clone in CMV expression vector: NM_004507
Primers:  Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
OriGene genome-wide validated SYBR primer pairs: NM_004507
UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in
DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication
factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several
proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA
polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the
primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1
cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths;
and DNA ligase I (LIG1) on long-patch base excision repair substratesGenatlas biochemistry entry for HUS1:cell cycle G2 checkpoint control gene,homologous to S pombe Hos18 MGI mutant phenotypes (inferred from 4 alleles ) (MGI details for Hus1):
2 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions
for HUS1
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
1 Sigma-Aldrich "Your Favorite Gene" Pathway for HUS1 (Your Favorite Gene powered by Ingenuity)
Gene Network CentralTM Interacting Genes and Proteins Network for HUS1
5/21 Interacting proteins for HUS1 (ENSP000002587743 O609211, 2) via UniProtKB, MINT, and/or STRING (see all 21
)About this table
5/9 Gene Ontology (GO) biological process terms (links to tree view) (see all 9
): About this table
|
Drugs & Compounds
for HUS1(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for HUS1
|
Transcripts
for HUS1(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene,
Expression Assays from Applied Biosystems)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004507
Sigma-Aldrich siRNA for HUS1
Sigma-Aldrich shRNA Panels and shRNA for HUS1
Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer® siRNAs: NM_004507 REFSEQ mRNAs for HUS1 gene: NM_004507.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_004507
OriGene GFP tagged cDNA clone in CMV expression vector: NM_004507
Myc/DDK tagged cDNA clone in CMV expression vector: NM_004507
untagged cDNA clone in CMV expression vector: NM_004507
Additional cDNA sequence: AJ227901.1 AK097182.1 AK290459.1 AK294117.1 BC007013.1 BT019481.1 BT019482.1 CR536552.1 CR619988.1 Y16893.1 8 DOTS entries: DT.310408 DT.70103554 DT.92424430 DT.95259816 DT.102823648 DT.92424429 DT.91652334 DT.95362322 24/87 AceView cDNA sequences (see all 87
):BX500811 AW132130 AI538328 AI167268 CR619988 CR536552 NM_004507 R95460 BU193379 BF222727 AA828114 CA418558 AA854956 BQ646867 BC007013 AL554895 BU932644 CA438762 AA353895 BG330719 AA693873 AA773515 BU171912 CD104810
 highest scoring ESTs for HUS1:AF076844 AA280710 AA353895 AI968626 BC007013 BE543378 BE568470 BE874516 BE892098 BF185772 Unigene Cluster for HUS1: HUS1 checkpoint homolog (S. pombe) Hs.152983 [show with all ESTs]Unigene Representative Sequence: CR619988
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for HUS1
| ExUns: | 1a | · | 1b | ^ | 2a | · | 2b | ^ | 3a | · | 3b | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8a | · | 8b | · | 8c | ^ | 9 | ^ | 10 | ^ | 11 |
|---|
|
| SP1: | | | | | - | | | | | | - | | | | - | | | | - | | | | | | - | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | |
| SP3: | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for HUS1
1 Ensembl transcript including schematic representation: ENST00000258774
|
Expression
for HUS1
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| HUS1 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for HUS1
1 / 2 / 3 6 probe-sets matching HUS1 gene Data from
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: --
SOURCE GeneReport for Unigene cluster: Hs.152983
Expression variation in blood from EXPOLDB for HUS1 UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921Tissue specificity: Ubiquitous |
Orthologs
for HUS1
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for HUS1 gene from 5/14 species (see all 14
)
About this table Species with no ortholog for HUS1
ENSEMBL Gene Tree for HUS1 | Paralogs
for HUS1(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for HUS1 gene
- HUS1B2
|
SNPs/Variants
for HUS1(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for HUS1 (up to first 250kb)
|
Disorders & Mutations
for HUS1
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 603760
2  Novoseek disease relationships for HUS1 gene
About this table
Human Genome Epidemiology Navigator: HUS1 (3 documents)
|
Medical News
for HUS1(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for HUS1 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/56 PubMed articles for HUS1 gene (see all 56
):- cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster. (PubMed id 9878245)1, 2, 3, 4 Dean F.B.... O'Donnell M. (1998)
- Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage. (PubMed id 9524127)2, 3, 4 Kostrub C.F....Enoch T. (1998)
- The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9. (PubMed id 10884395)1, 3, 4 Rauen M.... Karnitz L.M. (2000)
- HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. (PubMed id 10846170)1, 3, 4 Cai R.L.... Cohen D. (2000)
- PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. (PubMed id 11077446)1, 3, 4 Komatsu K.... Wang H.-G. (2000)
- The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1. (PubMed id 10359610)1, 3, 4 St Onge R.P.... Davey S. (1999)
- Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. (PubMed id 15897895)1, 3, 4 Wu X.... Zou Y. (2005)
- The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair. (PubMed id 15314187)1, 3, 4 Toueille M....Huebscher U. (2004)
- The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery. (PubMed id 15871698)3, 4 Smirnova E.... Huebscher U. (2005)
- The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity. (PubMed id 16216273)3, 4 Friedrich-Heineken E.... Huebscher U. (2005)
|
Search for HUS1
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing HUS1
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing HUS1
(According to HUGE)
About This Section
| -- |
Specialized Databases showing HUS1(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| About This Section
| --
| Services
for HUS1(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section
| 
Products for HUS1:
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