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Aliases for HPSE Gene

Aliases for HPSE Gene

  • Heparanase 2 3
  • HSE1 3 4 6
  • HPA 3 4 6
  • Endo-Glucoronidase 3 4
  • Heparanase-1 3 4
  • HPSE1 3 4
  • HPA1 3 4
  • HPR1 3 4
  • EC 3.2.1.166 4
  • HEP 4

External Ids for HPSE Gene

Previous GeneCards Identifiers for HPSE Gene

  • GC04M084138
  • GC04M084507
  • GC04M084672
  • GC04M084570
  • GC04M084435
  • GC04M084213
  • GC04M079959

Summaries for HPSE Gene

Entrez Gene Summary for HPSE Gene

  • Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

GeneCards Summary for HPSE Gene

HPSE (Heparanase) is a Protein Coding gene. Diseases associated with HPSE include gallbladder cancer and mucosal melanoma. Among its related pathways are Proteoglycans in cancer and Disease. GO annotations related to this gene include protein dimerization activity and beta-glucuronidase activity. An important paralog of this gene is HPSE2.

UniProtKB/Swiss-Prot for HPSE Gene

  • Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

Tocris Summary for HPSE Gene

  • Heparanases are endo beta-D-glucuronidase enzymes that cleave heparan sulfate side chains of heparan sulfate proteoglycans on cell surfaces and the extracellular matrix. Heparanases are involved in the clotting pathway and interact with tissue factor (TF) and TF pathway inhibitor (TFPI) to promote coagulation. Additional physiological roles include heparan sulfate turnover, embryo development, hair growth, and wound healing. Overexpression of heparanases is strongly implicated in tumor metastasis and angiogenesis

Gene Wiki entry for HPSE Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HPSE Gene

Genomics for HPSE Gene

Regulatory Elements for HPSE Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for HPSE Gene

Start:
83,292,461 bp from pter
End:
83,335,153 bp from pter
Size:
42,693 bases
Orientation:
Minus strand

Genomic View for HPSE Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for HPSE Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for HPSE Gene

Proteins for HPSE Gene

  • Protein details for HPSE Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y251-HPSE_HUMAN
    Recommended name:
    Heparanase
    Protein Accession:
    Q9Y251
    Secondary Accessions:
    • A9JIG7
    • C7F7I3
    • C7F7I4
    • E9PCA9
    • E9PGR1
    • Q53GE5
    • Q9UL39

    Protein attributes for HPSE Gene

    Size:
    543 amino acids
    Molecular mass:
    61149 Da
    Quaternary structure:
    • Heterodimer; heterodimer formation between the 8 kDa and the 50 kDa subunits is required for enzyme activity. Interacts with TF; the interaction, inhibited by heparin, enhances the generation of activated factor X and activates coagulation. Interacts with HRG; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity. Interacts with SDC1; the interaction enhances the shedding of SDC1. Interacts with HPSE2.

    Alternative splice isoforms for HPSE Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for HPSE Gene

Proteomics data for HPSE Gene at MOPED

Post-translational modifications for HPSE Gene

  • N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
  • Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
  • Glycosylation at Asn162, Asn178, Asn200, Asn217, Asn238, and Asn459
  • Modification sites at PhosphoSitePlus

Other Protein References for HPSE Gene

No data available for DME Specific Peptides for HPSE Gene

Domains for HPSE Gene

Protein Domains for HPSE Gene

Suggested Antigen Peptide Sequences for HPSE Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9Y251

UniProtKB/Swiss-Prot:

HPSE_HUMAN :
  • Q9Y251
Family:
  • Belongs to the glycosyl hydrolase 79 family.
genes like me logo Genes that share domains with HPSE: view

No data available for Gene Families for HPSE Gene

Function for HPSE Gene

Molecular function for HPSE Gene

GENATLAS Biochemistry: heparanase,widely expressed,noteworthy in placenta,platelet,spleen,smooth muscle cells,endoglyosidase cleaving heparan sulfate proteoglycan at a limited mumber of sites along the polysaccharide chain,essential in the disassembly of the extracellular matrix (ECM) by invading cells,involved in tumor invasion and metastasis
UniProtKB/Swiss-Prot BiophysicochemicalProperties: pH dependence: Optimum pH is 4-6. {ECO:0000269 PubMed:10446189, ECO:0000269 PubMed:10764835, ECO:0000269 PubMed:12713442, ECO:0000269 PubMed:15848168};
UniProtKB/Swiss-Prot CatalyticActivity: Endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan.
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by EDTA, laminarin sulfate and, to a lower extent, by heparin and sulfamin and activated by calcium and magnesium.
UniProtKB/Swiss-Prot Function: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

Enzyme Numbers (IUBMB) for HPSE Gene

Gene Ontology (GO) - Molecular Function for HPSE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004566 beta-glucuronidase activity TAS 10395325
GO:0005515 protein binding IPI 15292202
GO:0016798 hydrolase activity, acting on glycosyl bonds --
GO:0030305 heparanase activity TAS --
GO:0045545 syndecan binding IDA 15292202
genes like me logo Genes that share ontologies with HPSE: view
genes like me logo Genes that share phenotypes with HPSE: view

Animal Models for HPSE Gene

MGI Knock Outs for HPSE:

Animal Model Products

CRISPR Products

miRNA for HPSE Gene

miRTarBase miRNAs that target HPSE

No data available for Transcription Factor Targeting and HOMER Transcription for HPSE Gene

Localization for HPSE Gene

Subcellular locations from UniProtKB/Swiss-Prot for HPSE Gene

Lysosome membrane; Peripheral membrane protein. Secreted. Nucleus. Note=Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Associates with lipid rafts. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for HPSE Gene COMPARTMENTS Subcellular localization image for HPSE gene
Compartment Confidence
extracellular 5
lysosome 5
nucleus 5
vacuole 5
golgi apparatus 2
plasma membrane 2
endosome 1

Gene Ontology (GO) - Cellular Components for HPSE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region IEA --
GO:0005634 nucleus IDA 20309870
GO:0005654 nucleoplasm IDA --
GO:0005764 lysosome IDA 15126626
GO:0005765 lysosomal membrane IEA --
genes like me logo Genes that share ontologies with HPSE: view

Pathways for HPSE Gene

genes like me logo Genes that share pathways with HPSE: view

Pathways by source for HPSE Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Interacting Proteins for HPSE Gene

Gene Ontology (GO) - Biological Process for HPSE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process TAS --
GO:0006027 glycosaminoglycan catabolic process TAS --
GO:0006029 proteoglycan metabolic process TAS 10446189
GO:0007160 cell-matrix adhesion IDA 12773484
GO:0008152 metabolic process --
genes like me logo Genes that share ontologies with HPSE: view

Compounds for HPSE Gene

(5) Tocris Compounds for HPSE Gene

Compound Action Cas Number
2-Methoxyestradiol Apoptotic and antiangiogenic agent [362-07-2]
CHC Monocarboxylic acid transport (MCT) inhibitor [28166-41-8]
Herbimycin A Src family kinase inhibitor. Also Hsp90 inhibitor [70563-58-5]
Linomide Immunomodulator with antiangiogenic properties [84088-42-6]
P005091 USP7 inhibitor [882257-11-6]

(3) HMDB Compounds for HPSE Gene

Compound Synonyms Cas Number PubMed IDs
Calcium
  • Ca
7440-70-2
Heparin
  • Arteven
9005-49-6
Magnesium
  • Magnesium
7439-95-4

(2) Drugbank Compounds for HPSE Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
Dalteparin
  • Daltepariininatrium
9041-08-1 enzyme substrate
Heparin
  • Alpha-Heparin
9005-49-6 enzyme substrate

(36) Novoseek inferred chemical compound relationships for HPSE Gene

Compound -log(P) Hits PubMed IDs
heparan sulfate 94.9 222
matrigel 67.5 6
heparin 64.5 66
lysolecithin 49 2
vegf 47.6 45
genes like me logo Genes that share compounds with HPSE: view

Transcripts for HPSE Gene

Unigene Clusters for HPSE Gene

Heparanase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HPSE

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for HPSE Gene

No ASD Table

Relevant External Links for HPSE Gene

GeneLoc Exon Structure for
HPSE
ECgene alternative splicing isoforms for
HPSE

Expression for HPSE Gene

mRNA expression in normal human tissues for HPSE Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for HPSE Gene

This gene is overexpressed in Whole Blood (19.5).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for HPSE Gene

SOURCE GeneReport for Unigene cluster for HPSE Gene Hs.44227

mRNA Expression by UniProt/SwissProt for HPSE Gene

Q9Y251-HPSE_HUMAN
Tissue specificity: Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Also expressed in hair follicles, specifically in both Henles and Huxleys layers of inner the root sheath (IRS) at anagen phase.
genes like me logo Genes that share expressions with HPSE: view

In Situ Assay Products

Orthologs for HPSE Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for HPSE Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia HPSE 35
  • 99.32 (n)
  • 98.9 (a)
HPSE 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia HPSE 35
  • 86.32 (n)
  • 80.92 (a)
HPSE 36
  • 79 (a)
OneToOne
dog
(Canis familiaris)
Mammalia HPSE 35
  • 86.54 (n)
  • 83.08 (a)
HPSE 36
  • 81 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Hpse 35
  • 80.37 (n)
  • 78.08 (a)
Hpse 16
Hpse 36
  • 76 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia HPSE 36
  • 61 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Hpse 35
  • 79.91 (n)
  • 77.89 (a)
chicken
(Gallus gallus)
Aves HPSE 35
  • 62.57 (n)
  • 61.98 (a)
HPSE 36
  • 62 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia HPSE 36
  • 62 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia hpse 35
  • 61.96 (n)
  • 57.46 (a)
zebrafish
(Danio rerio)
Actinopterygii hpse 35
  • 57.03 (n)
  • 54.38 (a)
hpse 36
  • 50 (a)
OneToOne
thale cress
(Arabidopsis thaliana)
eudicotyledons GUS3 35
  • 44.35 (n)
  • 31.67 (a)
rice
(Oryza sativa)
Liliopsida Os06g0179000 35
  • 44.35 (n)
  • 35.04 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 40 (a)
OneToMany
Species with no ortholog for HPSE:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for HPSE Gene

ENSEMBL:
Gene Tree for HPSE (if available)
TreeFam:
Gene Tree for HPSE (if available)

Paralogs for HPSE Gene

Paralogs for HPSE Gene

Selected SIMAP similar genes for HPSE Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with HPSE: view

Variants for HPSE Gene

Sequence variations from dbSNP and Humsavar for HPSE Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs11031 -- 83,301,055(+) GTGAC(A/G)TTATG synonymous-codon, reference
rs4266284 -- 83,298,490(+) AAAAA(A/C)CAGAT intron-variant
rs4285078 -- 83,302,308(+) TAGAT(C/G)TTTAC intron-variant
rs4285079 -- 83,302,450(+) TCTGA(A/G)AGCAG intron-variant
rs4320127 -- 83,302,998(+) atctt(A/T)aaaaa intron-variant

Structural Variations from Database of Genomic Variants (DGV) for HPSE Gene

Variant ID Type Subtype PubMed ID
nsv526041 CNV Loss 19592680
nsv829992 CNV Loss 17160897
esv2600138 CNV Deletion 19546169
esv2727902 CNV Deletion 23290073
esv2180695 CNV Deletion 18987734
esv2727903 CNV Deletion 23290073
esv4639 CNV Deletion 18987735
esv6001 CNV Loss 19470904
esv2667959 CNV Deletion 23128226
dgv156n6 CNV Loss 16902084
esv1009126 CNV Deletion 20482838
esv2448972 CNV Deletion 19546169
esv1199239 CNV Deletion 17803354

Relevant External Links for HPSE Gene

HapMap Linkage Disequilibrium report
HPSE
Human Gene Mutation Database (HGMD)
HPSE

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HPSE Gene

Disorders for HPSE Gene

MalaCards: The human disease database

MalaCards: The human disease database. (8) Diseases for HPSE Gene including...

Search for HPSE Gene in MalaCards »

(1) University of Copenhagen DISEASES for HPSE Gene

(71) Novoseek inferred disease relationships for HPSE Gene

Disease -log(P) Hits PubMed IDs
metastasis 76.6 172
tumors 64.2 263
cancer 59.6 133
metastatic cancer 56.2 11
melanoma 53.3 70

Relevant External Links for HPSE

Genetic Association Database (GAD)
HPSE
Human Genome Epidemiology (HuGE) Navigator
HPSE
genes like me logo Genes that share disorders with HPSE: view

No data available for UniProtKB/Swiss-Prot for HPSE Gene

Publications for HPSE Gene

  1. Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis. (PMID: 10395325) Vlodavsky I. … Pecker I. (Nat. Med. 1999) 2 3 4 23
  2. Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis. (PMID: 10395326) Hulett M.D. … Parish C.R. (Nat. Med. 1999) 2 3 4 23
  3. Human heparanase. Purification, characterization, cloning, and expression. (PMID: 10446189) Toyoshima M. … Nakajima M. (J. Biol. Chem. 1999) 3 4 23
  4. Heparanase expression in invasive trophoblasts and acute vascular damage. (PMID: 10764835) Dempsey L.A. … Platt J.L. (Glycobiology 2000) 3 4 23
  5. Identification of active-site residues of the pro-metastatic endoglycosidase heparanase. (PMID: 11123890) Hulett M.D. … Parish C.R. (Biochemistry 2000) 3 4 23

Products for HPSE Gene

  • Addgene plasmids for HPSE

Sources for HPSE Gene

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