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Aliases for HPGDS Gene

Aliases for HPGDS Gene

  • Hematopoietic Prostaglandin D Synthase 2 3 5
  • Glutathione-Requiring Prostaglandin D Synthase 3 4
  • Glutathione-Dependent PGD Synthase 3 4
  • Glutathione S-Transferase Sigma 2 3
  • Prostaglandin-H2 D-Isomerase 3 4
  • GST Class-Sigma 3 4
  • PGDS 3 4
  • GSTS 3 4
  • Hematopoietic Prostaglandin D2 Synthase 3
  • Glutathione-Dependent PGD Synthetase 3
  • Glutathione S-Transferase 4
  • EC 5.3.99.2 4
  • EC 2.5.1.18 4
  • GSTS1-1 3
  • H-PGDS 4
  • PTGDS2 4
  • GSTS1 3
  • PGD2 3

External Ids for HPGDS Gene

Previous GeneCards Identifiers for HPGDS Gene

  • GC04M095439
  • GC04M095219
  • GC04M090957

Summaries for HPGDS Gene

Entrez Gene Summary for HPGDS Gene

  • Prostaglandin-D synthase is a sigma class glutathione-S-transferase family member. The enzyme catalyzes the conversion of PGH2 to PGD2 and plays a role in the production of prostanoids in the immune system and mast cells. The presence of this enzyme can be used to identify the differentiation stage of human megakaryocytes. [provided by RefSeq, Jul 2008]

GeneCards Summary for HPGDS Gene

HPGDS (Hematopoietic Prostaglandin D Synthase) is a Protein Coding gene. Diseases associated with HPGDS include Prostate Leiomyosarcoma and Baylisascariasis. Among its related pathways are Ibuprofen Pathway, Pharmacodynamics and Arachidonic acid metabolism. GO annotations related to this gene include calcium ion binding and magnesium ion binding. An important paralog of this gene is GSTA3.

UniProtKB/Swiss-Prot for HPGDS Gene

  • Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide.

Gene Wiki entry for HPGDS Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HPGDS Gene

Genomics for HPGDS Gene

Regulatory Elements for HPGDS Gene

Enhancers for HPGDS Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH04G094349 0.9 FANTOM5 dbSUPER 42.3 -6.5 -6497 0.2 SMARCE1 JUN FOSL1 JUND PRDM6 SMARCA4 ATF3 FOS TCF7L2 HPGDS PDLIM5 RPL35AP11
GH04G094338 0.9 FANTOM5 ENCODE 9.3 +3.7 3693 0.8 MAFG JUNB DPF2 RELA ZNF316 GATA2 HMBOX1 NFE2 MAFK EMSY HPGDS GC04M094330
GH04G094546 1.8 FANTOM5 Ensembl ENCODE dbSUPER 4.6 -206.8 -206780 5.8 PKNOX1 FOXA2 ATF1 ARNT E2F7 TCF12 GATA2 FOS SP5 NCOA1 PDLIM5 HPGDS PIR44106
GH04G094351 0.7 FANTOM5 Ensembl 8.2 -9.3 -9349 0.5 GATA2 POLR2A HPGDS RPL35AP11
GH04G094345 0.8 Ensembl dbSUPER 7.1 -2.8 -2825 1.0 JUND PKNOX1 FOS PRDM10 HPGDS RPL35AP11
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around HPGDS on UCSC Golden Path with GeneCards custom track

Genomic Location for HPGDS Gene

Chromosome:
4
Start:
94,298,535 bp from pter
End:
94,342,876 bp from pter
Size:
44,342 bases
Orientation:
Minus strand

Genomic View for HPGDS Gene

Genes around HPGDS on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
HPGDS Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for HPGDS Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for HPGDS Gene

Proteins for HPGDS Gene

  • Protein details for HPGDS Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O60760-HPGDS_HUMAN
    Recommended name:
    Hematopoietic prostaglandin D synthase
    Protein Accession:
    O60760
    Secondary Accessions:
    • Q6FHT9

    Protein attributes for HPGDS Gene

    Size:
    199 amino acids
    Molecular mass:
    23344 Da
    Cofactor:
    Name=glutathione; Xref=ChEBI:CHEBI:57925;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for HPGDS Gene

neXtProt entry for HPGDS Gene

Post-translational modifications for HPGDS Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for HPGDS Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • R&D Systems Antibodies for HPGDS (Hematopoietic Prostaglandin D Synthase/HPGDS)
  • Cloud-Clone Corp. Antibodies for HPGDS

Protein Products

No data available for DME Specific Peptides for HPGDS Gene

Domains & Families for HPGDS Gene

Gene Families for HPGDS Gene

Suggested Antigen Peptide Sequences for HPGDS Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O60760

UniProtKB/Swiss-Prot:

HPGDS_HUMAN :
  • Belongs to the GST superfamily. Sigma family.
Family:
  • Belongs to the GST superfamily. Sigma family.
genes like me logo Genes that share domains with HPGDS: view

Function for HPGDS Gene

Molecular function for HPGDS Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=8 mM for glutathione for the glutathione-conjugating activity {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; KM=0.6 mM for glutathione for the prostaglandin D synthase activity in the presence of EDTA {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; KM=0.14 mM for glutathione for the prostaglandin D synthase activity in the presence of magnesium ions {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=8.6 umol/min/mg enzyme with 1-bromo-2,4-dinitrobenzene as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=5.1 umol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=44.3 umol/min/mg enzyme with 1-fluoro-2,4-dinitrobenzene as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=10.7 umol/min/mg enzyme with 1-iodo-2,4-dinitrobenzene as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=6.8 umol/min/mg enzyme with allyl isothiocyanate as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=6.3 umol/min/mg enzyme with benzyl isothiocyanate as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825}; Vmax=0.05 umol/min/mg enzyme with cumene hydroperoxide as substrate {ECO:0000269 PubMed:11672424, ECO:0000269 PubMed:12627223, ECO:0000269 PubMed:15113825};
UniProtKB/Swiss-Prot CatalyticActivity:
(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.
UniProtKB/Swiss-Prot CatalyticActivity:
RX + glutathione = HX + R-S-glutathione.
UniProtKB/Swiss-Prot EnzymeRegulation:
Prostaglandin PGD2 synthesis is stimulated by calcium and magnesium ions. One calcium or magnesium ion is bound between the subunits of the homodimer. The interactions with the protein are for the most part mediated via water molecules. Magnesium increases the affinity for glutathione, while calcium has no effect on the affinity for glutathione.
UniProtKB/Swiss-Prot Function:
Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide.
UniProtKB/Swiss-Prot Induction:
By 12-O-tetradecanoylphorbol-13-acetate (TPA).

Enzyme Numbers (IUBMB) for HPGDS Gene

Gene Ontology (GO) - Molecular Function for HPGDS Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IDA 12627223
GO:0004364 glutathione transferase activity TAS --
GO:0004667 prostaglandin-D synthase activity IDA,TAS 12627223
GO:0005509 calcium ion binding IDA 12627223
GO:0005515 protein binding IPI 25416956
genes like me logo Genes that share ontologies with HPGDS: view
genes like me logo Genes that share phenotypes with HPGDS: view

Animal Models for HPGDS Gene

MGI Knock Outs for HPGDS:

Animal Model Products

  • Taconic Biosciences Mouse Models for HPGDS

Inhibitory RNA Products

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for HPGDS Gene

Localization for HPGDS Gene

Subcellular locations from UniProtKB/Swiss-Prot for HPGDS Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for HPGDS gene
Compartment Confidence
cytosol 5
mitochondrion 3
nucleus 3
plasma membrane 2
extracellular 2
cytoskeleton 2
peroxisome 2
endoplasmic reticulum 2
golgi apparatus 2
endosome 2
lysosome 1

Gene Ontology (GO) - Cellular Components for HPGDS Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm TAS 9353279
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with HPGDS: view

Pathways & Interactions for HPGDS Gene

genes like me logo Genes that share pathways with HPGDS: view

Gene Ontology (GO) - Biological Process for HPGDS Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001516 prostaglandin biosynthetic process IEA --
GO:0006629 lipid metabolic process IEA --
GO:0006631 fatty acid metabolic process IEA --
GO:0006633 fatty acid biosynthetic process IEA --
GO:0006693 prostaglandin metabolic process IDA,IEA 12627223
genes like me logo Genes that share ontologies with HPGDS: view

No data available for SIGNOR curated interactions for HPGDS Gene

Drugs & Compounds for HPGDS Gene

(14) Drugs for HPGDS Gene - From: DrugBank, ApexBio, DGIdb, and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Glycerol Approved, Experimental Pharma Target 238
Glutathione Approved Nutra Target 0
Tranilast Approved, Investigational Pharma Target Angiogenesis inhibitor 7
1-PHENYL-1H-PYRAZOLE-4-CARBOXYLIC ACID Experimental Pharma Target 0
2-(2f-Benzothiazolyl)-5-Styryl-3-(4f-Phthalhydrazidyl)Tetrazolium Chloride Experimental Pharma Target 0

(2) Additional Compounds for HPGDS Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Prostaglandin D2
  • (5Z,13E)-(15S)-9,15-dihydroxy-11-oxoprosta-5,13-dienoate
  • (5Z,13E)-(15S)-9,15-dihydroxy-11-oxoprosta-5,13-dienoic acid
  • (5z,13e)-(15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
  • (5z,13e)-(15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoic acid
  • (5Z,13E)-(15S)-9a,15-Dihydroxy-11-oxoprosta-5,13-dienoate
41598-07-6
Prostaglandin H2
  • (15S)Hydroxy-9alpha,11alpha-(epoxymethano)prosta-5,13-dienoate
  • (15S)Hydroxy-9alpha,11alpha-(epoxymethano)prosta-5,13-dienoic acid
  • (5Z)-7-{(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl}hept-5-enoate
  • (5Z)-7-{(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl}hept-5-enoic acid
  • (5Z,13E)-(15S)-9,11-epidioxy-15-hydroxyprosta-5,13-dienoate
42935-17-1

(2) ApexBio Compounds for HPGDS Gene

Compound Action Cas Number
HPGDS inhibitor 1 HPGDS inhibitor 1033836-12-2
HQL 79 162641-16-9
genes like me logo Genes that share compounds with HPGDS: view

Drug Products

Transcripts for HPGDS Gene

Unigene Clusters for HPGDS Gene

Hematopoietic prostaglandin D synthase:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for HPGDS Gene

No ASD Table

Relevant External Links for HPGDS Gene

GeneLoc Exon Structure for
HPGDS
ECgene alternative splicing isoforms for
HPGDS

Expression for HPGDS Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for HPGDS Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for HPGDS Gene

This gene is overexpressed in Oral epithelium (13.5), Pancreas (8.4), Stomach (7.6), and Lung (7.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for HPGDS Gene



Protein tissue co-expression partners for HPGDS Gene

NURSA nuclear receptor signaling pathways regulating expression of HPGDS Gene:

HPGDS

SOURCE GeneReport for Unigene cluster for HPGDS Gene:

Hs.128433

mRNA Expression by UniProt/SwissProt for HPGDS Gene:

O60760-HPGDS_HUMAN
Tissue specificity: Expressed in a number of megakaryocytic cell lines but not in platelets. Highly expressed in adipose tissue, macrophages and placenta. Also expressed at lower levels in lung, heart, lymph nodes, appendix, bone marrow and fetal liver.

Evidence on tissue expression from TISSUES for HPGDS Gene

  • Blood(4.7)
  • Nervous system(4.6)
  • Liver(3.3)
  • Lung(3)
  • Intestine(2.9)
  • Kidney(2.9)
  • Heart(2.5)
  • Muscle(2.5)
  • Skin(2.1)
  • Stomach(2)
genes like me logo Genes that share expression patterns with HPGDS: view

Primer Products

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for HPGDS Gene

Orthologs for HPGDS Gene

This gene was present in the common ancestor of animals.

Orthologs for HPGDS Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia HPGDS 34 35
  • 99.16 (n)
dog
(Canis familiaris)
Mammalia HPGDS 34 35
  • 89.28 (n)
cow
(Bos Taurus)
Mammalia HPGDS 34 35
  • 88.11 (n)
rat
(Rattus norvegicus)
Mammalia Hpgds 34
  • 83.25 (n)
mouse
(Mus musculus)
Mammalia Hpgds 34 16 35
  • 82.24 (n)
oppossum
(Monodelphis domestica)
Mammalia HPGDS 35
  • 73 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia HPGDS 35
  • 71 (a)
OneToOne
chicken
(Gallus gallus)
Aves HPGDS 34 35
  • 69.35 (n)
tropical clawed frog
(Silurana tropicalis)
Amphibia hpgds 34
  • 61.26 (n)
fruit fly
(Drosophila melanogaster)
Insecta GstS1 34 35
  • 48.97 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta GST_ANOGA 34
  • 45.9 (n)
worm
(Caenorhabditis elegans)
Secernentea gst-5 34
  • 45.96 (n)
gst-11 35
  • 37 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 40 (a)
OneToMany
-- 35
  • 37 (a)
OneToMany
-- 35
  • 36 (a)
OneToMany
-- 35
  • 32 (a)
OneToMany
-- 35
  • 32 (a)
OneToMany
CSA.3517 35
  • 32 (a)
OneToMany
CSA.4286 35
  • 29 (a)
OneToMany
Species where no ortholog for HPGDS was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for HPGDS Gene

ENSEMBL:
Gene Tree for HPGDS (if available)
TreeFam:
Gene Tree for HPGDS (if available)

Paralogs for HPGDS Gene

Paralogs for HPGDS Gene

genes like me logo Genes that share paralogs with HPGDS: view

Variants for HPGDS Gene

Sequence variations from dbSNP and Humsavar for HPGDS Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs1000055882 -- 94,314,992(+) AACGC(A/G)CAGTG intron-variant
rs1000253701 -- 94,319,719(+) TGGTG(A/G)TACAT intron-variant
rs1000281622 -- 94,319,914(+) CTCTA(A/C)CCCCA intron-variant
rs1000483679 -- 94,323,914(+) TTTCC(A/G)TGTTT intron-variant
rs1000533064 -- 94,315,183(+) CAATC(C/T)CCGGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for HPGDS Gene

Variant ID Type Subtype PubMed ID
esv3601448 CNV loss 21293372
nsv594901 CNV gain 21841781

Variation tolerance for HPGDS Gene

Residual Variation Intolerance Score: 76.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.11; 51.10% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for HPGDS Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
HPGDS

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HPGDS Gene

Disorders for HPGDS Gene

MalaCards: The human disease database

(16) MalaCards diseases for HPGDS Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
prostate leiomyosarcoma
  • leiomyosarcoma of the prostate
baylisascariasis
  • infection by baylisascaris
dracunculiasis
  • dracontiasis
scapuloperoneal myopathy
corneal dystrophy, subepithelial mucinous
  • subepithelial mucinous corneal dystrophy
- elite association - COSMIC cancer census association via MalaCards
Search HPGDS in MalaCards View complete list of genes associated with diseases

Relevant External Links for HPGDS

Genetic Association Database (GAD)
HPGDS
Human Genome Epidemiology (HuGE) Navigator
HPGDS
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
HPGDS
genes like me logo Genes that share disorders with HPGDS: view

No data available for UniProtKB/Swiss-Prot and Genatlas for HPGDS Gene

Publications for HPGDS Gene

  1. Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH- dependent prostaglandin D2 synthases. (PMID: 11672424) Jowsey I.R. … Hayes J.D. (Biochem. J. 2001) 2 3 4 22 64
  2. Novel prostaglandin D synthase inhibitors generated by fragment-based drug design. (PMID: 18341273) Hohwy M. … Edman K. (J. Med. Chem. 2008) 3 4 22 64
  3. First determination of the inhibitor complex structure of human hematopoietic prostaglandin D synthase. (PMID: 15113825) Inoue T. … Urade Y. (J. Biochem. 2004) 3 4 25 64
  4. Structure and chromosomal localization of human and mouse genes for hematopoietic prostaglandin D synthase. (PMID: 10824118) Kanaoka Y. … Hayaishi O. (Eur. J. Biochem. 2000) 3 4 22 64
  5. Prostaglandin D synthase in human megakaryoblastic cells. (PMID: 9353279) Mahmud I. … Hayaishi O. (J. Biol. Chem. 1997) 2 3 4 64

Products for HPGDS Gene

Sources for HPGDS Gene

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