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TLX1 Gene

protein-coding   GIFtS: 54

GC10P102880
T-cell leukemia homeobox 1
(Previous names: homeo box 11 (T-cell lymphoma 3-associated breakpoint), T-cell leukemia, homeobox 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: TCL3, HOX11)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
HOX11 2, 3, 5
MGC163402 2
OTTHUMP00000020816 2
TCL3 2, 3, 5
Descriptions
Homeo box-11 (T-cell leukemia-3 associated breakpoint,
homologous to Drosophila Notch) 2
Homeobox protein Hox-11 3
Proto-oncogene TCL-3 3
T-cell leukemia homeobox 1 2
T-cell leukemia, homeobox 1 1, 2
T-cell leukemia/lymphoma protein 3 3
homeo box 11 (T-cell lymphoma 3-associated breakpoint) 1, 2
External Ids
HGNC: 50561
Entrez Gene: 31952
UniProtKB: P313143
Ensembl: ENSG000001078077
Search outside databases for aliases for TLX1 gene

Previous GC identifers: GC10P101783 GC10P102123 GC10P103021 GC10P102554

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: TLX1_HUMAN, P31314
Function: Controls the genesis of the spleen. Binds to the DNA sequence 5'-GGCGGTAAGTGG-3'

Gene Wiki entry for TLX1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TLX1 gene  

Entrez Gene cytogenetic band: 10q24   Ensembl cytogenetic band:  10q24.31   HGNC cytogenetic band: 10q24.32

TLX1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P102880:     (about GC identifiers)

Start:
102,880,252 bp from pter
End:
102,887,536 bp from pter
Size:
7,285 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000010.9  NT_030059.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TLX1_HUMAN, P31314 (See protein sequence)
Recommended Name: T-cell leukemia homeobox protein 1  
Size: 330 amino acids; 34365 Da
Subcellular location: Nucleus (Probable)
Miscellaneous: TLX1 is oriented in a head-to-head manner with TDI. Both genes share the same
promoter with robust bidirectional activity
Secondary accessions: O75699 Q5VXP2 Q9HCA0

REFSEQ proteins: NP_005512.1  

ENSEMBL proteins: 
ENSP00000359215 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (HOX11)
Browse Abnova for Human Recombinant Proteins
              OriGene Purified Recombinant Human Protein: TLX1 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
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Antibodies for TLX1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (HOX11), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (TLX1)
Search Novus for antibodies for TLX1

Assays for TLX1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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3 InterPro domains/families:
 IPR001356 Homeobox
 IPR017970 Homeobox_CS
 IPR012287 Homeodomain-rel


   GeneDecks  TLX1 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P31314

ProtoNet protein and cluster: P31314

1 Blocks protein family: IPB000747 'Homeobox' engrailed-type protein

UniProtKB/Swiss-Prot: TLX1_HUMAN, P31314
Similarity: Contains 1 homeobox DNA-binding domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TLX1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TLX1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005521

              Applied Biosystems Silencer® siRNAs for TLX1

              Sigma-Aldrich siRNA for TLX1  
                     Sigma-Aldrich shRNA for TLX1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005521
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005521
                                 untagged cDNA clone in CMV expression vector: NM_005521 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_005521

UniProtKB/Swiss-Prot: TLX1_HUMAN, P31314
Function: Controls the genesis of the spleen. Binds to the DNA sequence 5'-GGCGGTAAGTGG-3'

4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Tlx1):

embryogenesishematopoietic systemimmune systemnervous system

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003700 transcription factor activity IEA--
GO:0043565 sequence-specific DNA binding IEA--
GO:0046982 protein heterodimerization activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  TLX1 


5/23 Interacting proteins for TLX1 (ENSP000003592153) via UniProtKB, MINT, and/or STRING (see all 23 )
InteractantInteraction Details
GeneCardExternal ID(s)
ALDH1A1ENSP000002977853STRING (score=.947)
PAX2ENSP000003593173STRING (score=.945)
EYA1ENSP000003733963STRING (score=.934)
TAL1ENSP000003609503STRING (score=.933)
LMO2ENSP000002578183STRING (score=.922)
About this table

5/9 Gene Ontology (GO) biological process terms (links to tree view) (see all 9 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0007275 multicellular organismal development IEA--
GO:0007417 central nervous system development IEA--
GO:0008284 positive regulation of cell proliferation IEA--
GO:0030182 neuron differentiation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for TLX1
5 Novoseek chemical compound relationships for TLX1 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
af 10 74.78 1 16383239 (1)
oligonucleotide 27.97 6 15384172 (1), 7596820 (1), 10869550 (1), 1352396 (1)
threonine 20.02 3 15897879 (2), 7909304 (1)
zinc 0.00 1 9004130 (1)
tyrosine 0.00 3 7866997 (3)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TLX1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TLX1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005521

              Sigma-Aldrich siRNA for TLX1  
                     Sigma-Aldrich shRNA for TLX1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_005521  

REFSEQ mRNAs for TLX1 gene: 

NM_005521.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_005521  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005521
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_005521
                                 untagged cDNA clone in CMV expression vector: NM_005521 

Additional cDNA sequence: 

BC130530.1 M62626.1 M75952.1 S38742.1 

2 DOTS entries:

DT.108199  DT.121300709 

16 AceView cDNA sequences:

BF514683 M75952 BI837519 CB185049 BX108942 AW149660 S38742 AW149389 
AA007444 D57678 AA007490 BF513502 BV197636 NM_005521 AI346822 AA922708 

highest scoring ESTs for TLX1:

S38742 AA007444 AA007490 AW814964 BF514683 BX108942 M75952 AA922708 AI346822 AW149660 

Unigene Cluster for TLX1:

T-cell leukemia homeobox 1
Hs.89583  [show with all ESTs]
Unigene Representative Sequence: M62626


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for TLX1

ExUns: 1a · 1b ^ 2 ^ 3a · 3b · 3c · 3d ^ 4
SP1:              -     -                           
SP2:              -                                 
SP3:                    -                           

About this scheme

ECgene alternative splicing isoforms for TLX1

1 Ensembl transcript including schematic representation:
ENST00000370196  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

TLX1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for TLX1

1 / 2 / 3

3 probe-sets matching TLX1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
559_s_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 S38742 1.00 1.00 1.00 1

207179_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005521 0.60 1.00 0.82 1

207179_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  TLX1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.89583

Expression variation in blood from EXPOLDB for TLX1

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for TLX1 gene from 5/10 species (see all 10 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TLX11   -- T-cell leukemia homeobox 1 91.31(n)
96.67(a)
608311  XM_845505.1  XP_850598.1 
chimpanzee
(Pan troglodytes)
TLX11   -- T-cell leukemia homeobox 1 99.09(n)
99.09(a)
744853  XM_001169592.1  XP_001169592.1 
cow
(Bos taurus)
TLX11   -- T-cell leukemia homeobox 1 91.72(n)
95.76(a)
505304  XM_581571.2  XP_581571.1 
rat
(Rattus norvegicus)
Tlx11   -- T-cell leukemia, homeobox 1 90.1(n)
97.88(a)
499361  XM_574674.2  XP_574674.2 
mouse
(Mus musculus)
Tlx11, 5 19 (43.00 cM)5
T-cell leukemia, homeobox 11, 5 90.2(n)1
97.27(a)1
219081  NM_021901.21  NP_068701.11 
 AB0006815  AJ2517875  (see all 13)
About this table        Species with no ortholog for TLX1

ENSEMBL Gene Tree for TLX1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for TLX1 gene
TLX32  TLX22  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/25 NCBI SNPs in TLX1 are shown (see all 25 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 92)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 10 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs70901851,2
H102883953(+) CCCAAG/AAAGAA 1 K/K syn1 ese3 trp34Minor allele frequency- A:0.00EU EA WA 388
rs9463281,2
C,F,H,O102883335(+) GGGCCC/TTAATA 1 -- int18Minor allele frequency- T:0.19MN EU EA WA NA 738
rs124156701,2
C,F,H102883830(+) CCTGGG/AACGCT 1 -- int14Minor allele frequency- A:0.13EU EA WA 408
rs111908421,2
C,F,H102885447(+) ACAGGC/AAGCCT 1 -- int18Minor allele frequency- A:0.14EU EA WA 838
rs14163611,2
A,F,H102885302(-) CTCCTC/TCCCTA 1 -- int1 trp33Minor allele frequency- T:0.27EU EA 252
rs27420331,2
F,H102885211(+) CCTCAG/CACCTT 1 -- int14Minor allele frequency- C:0.01EU EA WA 406
rs27420291,2
F,H102884576(+) GCAGAG/AGGTCT 1 -- int13Minor allele frequency- A:0.01EU EA 280
rs27420221,2
H102883270(+) ACCCAG/ACCGGG 1 -- int14Minor allele frequency- A:0.00EU EA WA 414
rs27420181,2
H102883094(+) GTGGAG/AGCTGG 1 -- int14Minor allele frequency- A:0.00EU EA WA 408
rs27420301,2
H102884661(+) TGGAAG/AGAGAA 1 -- int1 trp34Minor allele frequency- A:0.00EU EA WA 404
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HapMap Linkage Disequilibrium images for TLX1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
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OMIM: 186770

UniProtKB/Swiss-Prot: TLX1_HUMAN, P31314

  • A chromosomal aberration involving TLX1 may be a cause of a form of T-cell acute
    lymphoblastic leukemia (T-ALL). Translocation t(10;14)(q24;q11) with TCRD
  • 8 Novoseek disease relationships for TLX1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    t-all 92.47 47 15384172 (3), 1717256 (2), 12750702 (2), 7478554 (2) (see all 26)
    leukemogenesis 73.56 6 11587205 (1), 8559142 (1), 15384172 (1), 17303350 (1) (see all 6)
    leukemia t-cell 71.95 10 1352396 (2), 7698766 (1), 1717256 (1), 8559142 (1) (see all 7)
    leukemia 52.09 22 14659882 (3), 14975618 (3), 10393251 (2), 9778044 (2) (see all 12)
    lymphoblastic leukemia acute childhood 40.15 1 7478554 (1)
    cancer 0.00 4 12750702 (2), 14659882 (1), 7719228 (1)
    tumors 0.00 8 11069299 (3), 7478554 (2), 18082256 (1), 9811890 (1)
    lymphoma 0.00 4 11069299 (3)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/78 PubMed articles for TLX1 gene (see all 78 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 3195 HGNC: 5056 AceView: TLX1 Ensembl:ENSG00000107807 euGenes: HUgn3195
    ECgene: TLX1 H-InvDB: TLX1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for TLX1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TLX1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for TLX1 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for TLX1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Recombinant Proteins
    (HOX11)
     Antibodies (HOX11)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for TLX1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for TLX1

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