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Aliases for HINT3 Gene

Aliases for HINT3 Gene

  • Histidine Triad Nucleotide Binding Protein 3 2 3
  • HINT-3 3 4
  • Histidine Triad Nucleotide-Binding Protein 3 3
  • HIT-Like Protein 3
  • EC 3.-.-.- 4
  • HINT4 3

External Ids for HINT3 Gene

Previous GeneCards Identifiers for HINT3 Gene

  • GC06P125937
  • GC06P126125
  • GC06P126258
  • GC06P126319
  • GC06P126277
  • GC06P123854

Summaries for HINT3 Gene

Entrez Gene Summary for HINT3 Gene

  • Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM, Mar 2008]

GeneCards Summary for HINT3 Gene

HINT3 (Histidine Triad Nucleotide Binding Protein 3) is a Protein Coding gene. GO annotations related to this gene include nucleotide binding and hydrolase activity.

UniProtKB/Swiss-Prot for HINT3 Gene

  • Hydrolyzes phosphoramidate and acyl-adenylate substrates.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HINT3 Gene

Genomics for HINT3 Gene

Regulatory Elements for HINT3 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for HINT3 Gene

125,956,715 bp from pter
125,980,244 bp from pter
23,530 bases
Plus strand

Genomic View for HINT3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for HINT3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for HINT3 Gene

Proteins for HINT3 Gene

  • Protein details for HINT3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Histidine triad nucleotide-binding protein 3
    Protein Accession:
    Secondary Accessions:
    • B3KQ91
    • Q8N0Y9

    Protein attributes for HINT3 Gene

    182 amino acids
    Molecular mass:
    20361 Da
    Quaternary structure:
    • Forms dimers to octamers and even larger oligomer

neXtProt entry for HINT3 Gene

Proteomics data for HINT3 Gene at MOPED

Post-translational modifications for HINT3 Gene

  • Ubiquitination at Lys78, Lys115, and Lys179
  • Modification sites at PhosphoSitePlus

Other Protein References for HINT3 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for HINT3 Gene

Domains for HINT3 Gene

Protein Domains for HINT3 Gene

Suggested Antigen Peptide Sequences for HINT3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 HIT domain.
  • Belongs to the HINT family.
  • Contains 1 HIT domain.
  • Belongs to the HINT family.
genes like me logo Genes that share domains with HINT3: view

No data available for Gene Families for HINT3 Gene

Function for HINT3 Gene

Molecular function for HINT3 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=1.5 uM for indolepropinoic acyl-adenylate {ECO:0000269 PubMed:17870088}; KM=22 uM for adenine {ECO:0000269 PubMed:17870088}; KM=29 uM for guanine {ECO:0000269 PubMed:17870088}; KM=32 uM for hypoxanthine {ECO:0000269 PubMed:17870088}; KM=121 uM for uracil {ECO:0000269 PubMed:17870088}; KM=181 uM for cytosine {ECO:0000269 PubMed:17870088}; Note=HINT3 prefers purine over pyrimidine-based substrates.;
UniProtKB/Swiss-Prot Function:
Hydrolyzes phosphoramidate and acyl-adenylate substrates.

Enzyme Numbers (IUBMB) for HINT3 Gene

Gene Ontology (GO) - Molecular Function for HINT3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003824 catalytic activity --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with HINT3: view

Phenotypes for HINT3 Gene

GenomeRNAi human phenotypes for HINT3:
genes like me logo Genes that share phenotypes with HINT3: view

Animal Model Products

miRNA for HINT3 Gene

miRTarBase miRNAs that target HINT3

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HINT3

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for HINT3 Gene

Localization for HINT3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for HINT3 Gene

Cytoplasm. Nucleus. Note=Localized as aggregates in the cytoplasm and the nucleus.

Subcellular locations from

Jensen Localization Image for HINT3 Gene COMPARTMENTS Subcellular localization image for HINT3 gene
Compartment Confidence
nucleus 5
mitochondrion 4
cytosol 3
extracellular 3

Gene Ontology (GO) - Cellular Components for HINT3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IDA --
GO:0005739 mitochondrion IDA --
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with HINT3: view

Pathways for HINT3 Gene

SuperPathways for HINT3 Gene

No Data Available

Interacting Proteins for HINT3 Gene

STRING Interaction Network Preview (showing 3 interactants - click image to see details)
Selected Interacting proteins: ENSP00000229633 for HINT3 Gene via STRING

Gene Ontology (GO) - Biological Process for HINT3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008152 metabolic process IEA --
genes like me logo Genes that share ontologies with HINT3: view

No data available for Pathways by source for HINT3 Gene

Transcripts for HINT3 Gene

mRNA/cDNA for HINT3 Gene

Unigene Clusters for HINT3 Gene

Histidine triad nucleotide binding protein 3:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HINT3

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for HINT3 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b
SP1: -
SP2: - -
SP3: -

Relevant External Links for HINT3 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for HINT3 Gene

mRNA expression in normal human tissues for HINT3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues for HINT3 Gene

This gene is overexpressed in Brain (10.5) and Frontal cortex (8.5).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for HINT3 Gene

SOURCE GeneReport for Unigene cluster for HINT3 Gene Hs.72325

genes like me logo Genes that share expressions with HINT3: view

Expression partners for HINT3 Gene

* - Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for HINT3 Gene

Orthologs for HINT3 Gene

This gene was present in the common ancestor of animals.

Orthologs for HINT3 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia HINT3 35
  • 80.26 (n)
  • 71.51 (a)
HINT3 36
  • 70 (a)
(Canis familiaris)
Mammalia HINT3 35
  • 89.86 (n)
  • 89.57 (a)
HINT3 36
  • 89 (a)
(Mus musculus)
Mammalia Hint3 35
  • 76.04 (n)
  • 70.62 (a)
Hint3 16
Hint3 36
  • 67 (a)
(Pan troglodytes)
Mammalia HINT3 35
  • 99.63 (n)
  • 99.45 (a)
HINT3 36
  • 99 (a)
(Rattus norvegicus)
Mammalia Hint3 35
  • 74.71 (n)
  • 69.54 (a)
(Monodelphis domestica)
Mammalia -- 36
  • 57 (a)
-- 36
  • 54 (a)
(Ornithorhynchus anatinus)
Mammalia HINT3 36
  • 74 (a)
(Gallus gallus)
Aves HINT3 35
  • 71.7 (n)
  • 66.19 (a)
HINT3 36
  • 56 (a)
(Anolis carolinensis)
Reptilia HINT3 36
  • 63 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia hint3 35
  • 65.78 (n)
  • 62.67 (a)
(Danio rerio)
Actinopterygii hint3 35
  • 60.44 (n)
  • 58 (a)
hint3 36
  • 54 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007577 35
  • 49.75 (n)
  • 37.12 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG34015 35
  • 52.49 (n)
  • 42.52 (a)
CG15362 36
  • 29 (a)
CG34015 36
  • 40 (a)
(Caenorhabditis elegans)
Secernentea hint-3 36
  • 26 (a)
Species with no ortholog for HINT3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for HINT3 Gene

Gene Tree for HINT3 (if available)
Gene Tree for HINT3 (if available)

Paralogs for HINT3 Gene

No data available for Paralogs for HINT3 Gene

Variants for HINT3 Gene

Sequence variations from dbSNP and Humsavar for HINT3 Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type MAF
rs941457 -- 125,971,041(-) AAAGA(C/G)GAAGA intron-variant
rs941459 -- 125,980,546(-) TGAAT(C/T)AACAA downstream-variant-500B
rs1883868 -- 125,976,698(-) CATAT(C/T)TTATA intron-variant
rs2024583 -- 125,974,599(-) CCTCA(A/G)TATAA intron-variant
rs2024584 -- 125,963,229(-) GGCAA(A/G)ACATC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for HINT3 Gene

Variant ID Type Subtype PubMed ID
nsv830796 CNV Loss 17160897
nsv886636 CNV Loss 21882294
esv2732681 CNV Deletion 23290073

Relevant External Links for HINT3 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HINT3 Gene

Disorders for HINT3 Gene

No disorders were found for HINT3 Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships , Genatlas and External Links for HINT3 Gene

Publications for HINT3 Gene

  1. Evidence that human histidine triad nucleotide binding protein 3 (Hint3) is a distinct branch of the histidine triad (HIT) superfamily. (PMID: 17870088) Chou T.-F. … Wagner C.R. (J. Mol. Biol. 2007) 3 4 23
  2. Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. (PMID: 11805111) Bieganowski P. … Brenner C. (J. Biol. Chem. 2002) 2 3
  3. Hint, Fhit, and GalT: function, structure, evolution, and mechanism of three branches of the histidine triad superfamily of nucleotide hydrolases and transferases. (PMID: 12119013) Brenner C. (Biochemistry 2002) 3 4
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  5. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PMID: 16344560) Kimura K. … Sugano S. (Genome Res. 2006) 3

Products for HINT3 Gene

Sources for HINT3 Gene

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