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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

HINT2 Gene

protein-coding   GIFtS: 47
GCID: GC09M035802

histidine triad nucleotide binding protein 2

 Explore 6 diseases affiliated with
HINT2 via our new
 Human Malady Compendium 
Biological research products
for HINT2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Histidine Triad Nucleotide Binding Protein 21 2     HIT-172
HINT-22 3     Histidine Triad Nucleotide-Binding Protein 2, Mitochondrial2
HINT-32 3     Protein Kinase C Inhibitor-22
HIT-17kDa2 3     EC 3.-.-.-3
PKCI-1-Related HIT Protein2 3     

External Ids:    HGNC: 183441   Entrez Gene: 846812   Ensembl: ENSG000001371337   OMIM: 6099975   UniProtKB: Q9BX683   

Export aliases for HINT2 gene to outside databases

Previous GC identifers: GC09M036125 GC09M035982


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for HINT2:
Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of
ribonucleotides (Brenner, 2002 (PubMed 12119013)).(supplied by OMIM, Mar 2008)

UniProtKB/Swiss-Prot: HINT2_HUMAN, Q9BX68
Function: Hydrolase probably involved in steroid biosynthesis. May play a role in apoptosis. Has adenosine
phosphoramidase activity




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000009.11  NC_018920.1  NT_008413.18  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the HINT2 gene promoter:
         Oct-B1   Egr-3   oct-B3   oct-B2   Lmo2   Evi-1   HNF-1A   AREB6   HNF-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidHINT2 promoter sequence
   Search SABiosciences Chromatin IP Primers for HINT2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HINT2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9p13.3   Ensembl cytogenetic band:  9p13.3   HGNC cytogenetic band: 9p11.2

HINT2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
HINT2 gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09M035802:  view genomic region     (about GC identifiers)

Start:
35,812,957 bp from pter      End:
35,815,351 bp from pter
Size:
2,395 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: HINT2_HUMAN, Q9BX68 (See protein sequence)
Recommended Name: Histidine triad nucleotide-binding protein 2, mitochondrial precursor  
Size: 163 amino acids; 17162 Da
Subcellular location: Mitochondrion
Secondary accessions: Q5TCW3

Explore the universe of human proteins at neXtProt for HINT2: NX_Q9BX68

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BX68

  • HINT2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_115982.1  
    ENSEMBL proteins: 
     ENSP00000259667  

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    Uscn Proteins for HINT2

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005739mitochondrion ISS--


    HINT2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    HINT2 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR001310 Histidine_triad_HIT
     IPR019808 Histidine_triad_CS
     IPR011146 HIT-like

    Graphical View of Domain Structure for InterPro Entry Q9BX68

    ProtoNet protein and cluster: Q9BX68

    1 Blocks protein family: IPB001310 Histidine triad (HIT) protein

    UniProtKB/Swiss-Prot: HINT2_HUMAN, Q9BX68
    Similarity: Belongs to the HINT family
    Similarity: Contains 1 HIT domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: HINT2_HUMAN, Q9BX68
    Function: Hydrolase probably involved in steroid biosynthesis. May play a role in apoptosis. Has adenosine
    phosphoramidase activity
    Biophysicochemical properties: Kinetic parameters: KM=128 uM for adenosine 5'-O-p-nitrophenylphosphoramidate;

    Enzyme Number (IUBMB): EC 3.-.-.-1

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016787hydrolase activity IEA--


    HINT2 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for HINT2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 7)

    5/7 Interacting proteins for HINT2 (ENSP000002596674) via UniProtKB, MINT, STRING, and/or I2D (see all 7)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PGDENSP000002707764STRING: ENSP00000270776
    PA2G4ENSP000003028864STRING: ENSP00000302886
    CACYBPENSP000003566524STRING: ENSP00000356652
    MNAT1ENSP000002612454STRING: ENSP00000261245
    SOD1ENSP000002701424STRING: ENSP00000270142
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006694steroid biosynthetic process IEA--
    GO:0006915apoptotic process IEA--


    HINT2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for HINT2
    Search CenterWatch for drugs/clinical trials and news about HINT2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for HINT2 gene: 
    NM_032593.2  

    Unigene Cluster for HINT2:

    Histidine triad nucleotide binding protein 2
    Hs.70573  [show with all ESTs]
    Unigene Representative Sequence: BC033538
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000490578 ENST00000472085 ENST00000259667 ENST00000474908(uc003zyh.3)
    ENST00000471774 ENST00000461169 ENST00000474848

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    OriGene shRNA RFP: HINT2
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HINT2
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    Additional cDNA sequence: 

    AF085236.1 AF356515.1 AF356875.1 AK222582.1 AK309795.1 AY033094.1 BC033538.1 BC047737.2 

    9 DOTS entries:

    DT.100779858  DT.114875  DT.101981509  DT.100779857  DT.100779855  DT.121165159  DT.100779856  DT.100010162 
    DT.95167360 

    24/112 AceView cDNA sequences (see all 112):

    NM_032593 BQ004789 AI800955 BC033538 BM764627 BE905154 AA599349 CD722582 
    AF356875 BU623621 AI083659 CD673269 CA391314 CD675786 BQ050545 AA563798 
    AF356515 AW103005 CA312502 AI817457 AI800967 BC047737 AI591234 BM987450 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for HINT2 (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c · 6d · 6e
    SP1:                          -     -                             -                           
    SP2:                                                              -                           
    SP3:                                                                                          
    SP4:                                            -                 -                           
    SP5:                                                                                          


    ECgene alternative splicing isoforms for HINT2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    HINT2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TACTTGGGGG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See HINT2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for HINT2

    SOURCE GeneReport for Unigene cluster: Hs.70573

    UniProtKB/Swiss-Prot: HINT2_HUMAN, Q9BX68
    Tissue specificity: High expression in liver and pancreas. Expression is significantly down-regulated in hepatocellular
    carcinoma (HCC) patients

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    In Situ
    Assay Products:
     

     
    Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for HINT2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the last universal common ancestor (LUCA).

    Orthologs for HINT2 gene from 6/27 species (see all 27)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves LOC1008591121 histidine triad nucleotide-binding protein 2, mitochondrial-like 66.67(n)
    70.51(a)
      100859112  XM_003642973.1  XP_003643021.1 
    lizard
    (Anolis carolinensis)
    Reptilia HINT26
    --
    80(a)
    1 ↔ 1
    AAWZ02039281(194-2120)
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.165482 Transcribed sequence with moderate similarity to protein more 71.69(n)    BI709975.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons HINT 21 histidine triad nucleotide-binding 2 protein 57.72(n)
    53.66(a)
      840001  NM_102854.4  NP_174401.1 
    rice
    (Oryza sativa)
    Liliopsida Os06g06675001 hypothetical protein 57.2(n)
    57.95(a)
      4341789  NM_001064849.1  NP_001058314.1 
    E. coli
    (Escherichia coli)
    Gamma proteobacteria hinT6
    purine nucleoside phosphoramidase, dadA activator ...
    44(a)
    1 → many
    Chromosome(1161108-1161467)


    ENSEMBL Gene Tree for HINT2 (if available)
    TreeFam Gene Tree for HINT2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for HINT2 gene
    HINT12  
    2 SIMAP similar genes for HINT2 using alignment to 1 protein entry:     HINT2_HUMAN:
    HINT1    LOC346521

    HINT2 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for HINT2
    PGOHUM00000237924


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/39 NCBI SNPs in HINT2 are shown (see all 39    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 9 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs455579331,2
    C,--35814270(+) TTTAAA/GTTTTA 1 -- int10--------
    rs1471023171,2
    --35814297(+) TGACAC/GTAGTT 1 -- int10--------
    rs1152210661,2
    C,F,--35814488(+) TTCTCT/CGACCT 1 -- int11Minor allele frequency- C:0.06WA 118
    rs577391961,2
    C,--35814520(+) CAGAAG/AAGAGG 1 -- int11Minor allele frequency- A:0.50WA 2
    rs1423260351,2
    --35814583(+) GCTCC-/AAGAAGAG 1 -- int10--------
    rs1869102741,2
    --35814649(+) AGAAGA/GCGGCA 1 -- int10--------
    rs1439624191,2
    --35814815(+) CGGCTC/TTGCGC 1 -- int10--------
    rs1929487631,2
    --35815042(+) GGTGGA/GGACTC 1 -- ut510--------
    rs1506742521,2
    --35815222(+) TTCGTC/GTTCTA 1 -- us2k10--------
    rs1397593191,2
    --35815286(+) AGAAAC/TTTCCA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for HINT2 (35812957 - 35815351 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for HINT2: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    HINT2 for disorders           About GeneDecksing

    OMIM gene information: 609997    OMIM disorders: --

    6 diseases for HINT2:    About MalaCards
    hepatocellular carcinoma    pneumonia    tuberculosis    carcinoma
    malaria    mycobacterium tuberculosis

    Human Genome Epidemiology (HuGE) Navigator: HINT2 (2 documents)

    Export disorders for HINT2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for HINT2 gene, integrated from 9 sources (see all 15):
    (articles sorted by number of sources associating them with HINT2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis. (PubMed id 18653718)1, 2 Lenglet S.... Rossier M.F. (2008)
    2. Hint2, a mitochondrial apoptotic sensitizer down-regulated in hepatocellular carcinoma. (PubMed id 16762638)1, 2 Martin J.... Dufour J.-F. (2006)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    5. Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
    6. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    7. Genetic variants in nuclear-encoded mitochondrial gen es influence AIDS progression. (PubMed id 20877624)1 Hendrickson S.L....O'Brien S.J. (2010)
    8. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (2007)
    9. Transcriptome analysis of human gastric cancer. (PubMed id 16341674)1 Oh J.H....Kim N.S. (2005)
    10. DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)2 Humphray S.J.... Dunham I. (2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 84681 HGNC: 18344 AceView: HINT2 Ensembl:ENSG00000137133 euGenes: HUgn84681
    ECgene: HINT2 H-InvDB: HINT2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for HINT2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for HINT2 gene:
    Search GeneIP for patents involving HINT2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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