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Aliases for HIF1AN Gene

Aliases for HIF1AN Gene

  • Hypoxia Inducible Factor 1, Alpha Subunit Inhibitor 2 3
  • Hypoxia-Inducible Factor Asparagine Hydroxylase 3 4
  • Peptide-Aspartate Beta-Dioxygenase 2 3
  • Factor Inhibiting HIF-1 3 4
  • FIH-1 3 4
  • FIH1 3 4
  • Factor Inhibiting HIF1 3
  • EC 1.14.11.30 4
  • EC 1.14.11.n4 4

External Ids for HIF1AN Gene

Previous GeneCards Identifiers for HIF1AN Gene

  • GC10P101188
  • GC10P101529
  • GC10P102420
  • GC10P101960
  • GC10P102285
  • GC10P095924

Summaries for HIF1AN Gene

GeneCards Summary for HIF1AN Gene

HIF1AN (Hypoxia Inducible Factor 1, Alpha Subunit Inhibitor) is a Protein Coding gene. Diseases associated with HIF1AN include hypoxia and severe pre-eclampsia. Among its related pathways are Angiogenesis (CST) and Cellular Senescence. GO annotations related to this gene include protein homodimerization activity and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors. An important paralog of this gene is HSPBAP1.

UniProtKB/Swiss-Prot for HIF1AN Gene

  • Hydroxylates HIF-1 alpha at Asp-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Gene Wiki entry for HIF1AN Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HIF1AN Gene

Genomics for HIF1AN Gene

Regulatory Elements for HIF1AN Gene

Genomic Location for HIF1AN Gene

Chromosome:
10
Start:
100,529,072 bp from pter
End:
100,559,998 bp from pter
Size:
30,927 bases
Orientation:
Plus strand

Genomic View for HIF1AN Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
GeneLoc Logo Gene Density

RefSeq DNA sequence for HIF1AN Gene

Proteins for HIF1AN Gene

  • Protein details for HIF1AN Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NWT6-HIF1N_HUMAN
    Recommended name:
    Hypoxia-inducible factor 1-alpha inhibitor
    Protein Accession:
    Q9NWT6
    Secondary Accessions:
    • D3DR69
    • Q5W147
    • Q969Q7
    • Q9NPV5

    Protein attributes for HIF1AN Gene

    Size:
    349 amino acids
    Molecular mass:
    40285 Da
    Cofactor:
    Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
    Quaternary structure:
    • Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4 (By similarity). Interacts with UBE3A. Interacts with ANKS3 (By similarity).

    Three dimensional structures from OCA and Proteopedia for HIF1AN Gene

neXtProt entry for HIF1AN Gene

Proteomics data for HIF1AN Gene at MOPED

Post-translational modifications for HIF1AN Gene

  • Ubiquitination at Lys 115 and Lys 131
  • Modification sites at PhosphoSitePlus

Other Protein References for HIF1AN Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • R&D Systems Antibodies for HIF1AN (FIH-1/HIF-1AN)
  • Cell Signaling Technology (CST) Antibodies for HIF1AN (HIF1AN)

No data available for DME Specific Peptides for HIF1AN Gene

Domains & Families for HIF1AN Gene

Protein Domains for HIF1AN Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for HIF1AN Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NWT6

UniProtKB/Swiss-Prot:

HIF1N_HUMAN :
  • Contains 1 JmjC domain.
Domain:
  • Contains 1 JmjC domain.
genes like me logo Genes that share domains with HIF1AN: view

No data available for Gene Families for HIF1AN Gene

Function for HIF1AN Gene

Molecular function for HIF1AN Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=100 uM for HIF1A (788-822) peptide {ECO:0000269 PubMed:14701857}; KM=160 uM for HIF2A (832-866) peptide {ECO:0000269 PubMed:14701857}; KM=0.5 uM for Fe(2+) {ECO:0000269 PubMed:14701857}; KM=25 uM for 2-oxoglutarate {ECO:0000269 PubMed:14701857}; KM=260 uM for ascorbate {ECO:0000269 PubMed:14701857}; KM=90 uM for O(2) {ECO:0000269 PubMed:14701857}; Note=The kinetic constants are determined for the recombinant FLAG-His-tagged protein.;
UniProtKB/Swiss-Prot CatalyticActivity:
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
UniProtKB/Swiss-Prot CatalyticActivity:
Ankyrin-repeat-L-histidine + 2-oxoglutarate + O(2) = ankyrin-repeat-(3S)-3-hydroxy-L-histidine + succinate + CO(2)
UniProtKB/Swiss-Prot Function:
Hydroxylates HIF-1 alpha at Asp-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Enzyme Numbers (IUBMB) for HIF1AN Gene

Gene Ontology (GO) - Molecular Function for HIF1AN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005112 Notch binding IPI 17573339
GO:0005506 iron ion binding IDA 12446723
GO:0005515 protein binding IPI 11641274
GO:0008270 zinc ion binding IDA 12446723
GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors EXP --
genes like me logo Genes that share ontologies with HIF1AN: view
genes like me logo Genes that share phenotypes with HIF1AN: view

Animal Models for HIF1AN Gene

MGI Knock Outs for HIF1AN:

Animal Model Products

CRISPR Products

miRNA for HIF1AN Gene

miRTarBase miRNAs that target HIF1AN

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HIF1AN

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for HIF1AN Gene

Localization for HIF1AN Gene

Subcellular locations from UniProtKB/Swiss-Prot for HIF1AN Gene

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for HIF1AN Gene COMPARTMENTS Subcellular localization image for HIF1AN gene
Compartment Confidence
nucleus 5
cytosol 4
mitochondrion 1

Gene Ontology (GO) - Cellular Components for HIF1AN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 colocalizes_with nucleus IDA 18299578
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IDA 14734545
GO:0005829 cytosol TAS --
GO:0048471 perinuclear region of cytoplasm IDA 19726677
genes like me logo Genes that share ontologies with HIF1AN: view

Pathways & Interactions for HIF1AN Gene

genes like me logo Genes that share pathways with HIF1AN: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

SIGNOR curated interactions for HIF1AN Gene

Inactivates:

Gene Ontology (GO) - Biological Process for HIF1AN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0036138 peptidyl-histidine hydroxylation IDA 21251231
GO:0042264 peptidyl-aspartic acid hydroxylation IDA 21177872
GO:0042265 peptidyl-asparagine hydroxylation IMP 19245366
GO:0045663 positive regulation of myoblast differentiation IDA 18299578
genes like me logo Genes that share ontologies with HIF1AN: view

Drugs & Compounds for HIF1AN Gene

(7) Drugs for HIF1AN Gene - From: HMDB, DrugBank, NovoSeek, and ApexBio

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Succinic acid Approved Nutra Full agonist, Agonist 0
D-tartaric acid Experimental Pharma Target 0
N-(carboxycarbonyl)-D-phenylalanine Experimental Pharma Target 0
BAY 87-2243 Pharma HIF-1 inhibitor,potent and selective 0
Cobalt Pharma 147

(7) Additional Compounds for HIF1AN Gene - From: NovoSeek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
oxygen
  • Dioxygen
  • Molecular oxygen
  • O2
  • Oxygen
  • Oxygen molecule
7782-44-7
carbon dioxide
  • Carbon oxide
  • Carbon-12 dioxide
  • Carbonic acid anhydride
  • Carbonic acid gas
  • Carbonic anhydride
124-38-9
Fe2+
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
15438-31-0
Oxoglutaric acid
  • 2-Ketoglutarate
  • 2-Ketoglutaric acid
  • 2-Oxo-1,5-pentanedioate
  • 2-Oxo-1,5-pentanedioic acid
  • 2-Oxoglutarate
328-50-7
alpha-ketoglutarate
genes like me logo Genes that share compounds with HIF1AN: view

Drug Products

Transcripts for HIF1AN Gene

Unigene Clusters for HIF1AN Gene

Hypoxia inducible factor 1, alpha subunit inhibitor:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for HIF1AN

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for HIF1AN Gene

No ASD Table

Relevant External Links for HIF1AN Gene

GeneLoc Exon Structure for
HIF1AN
ECgene alternative splicing isoforms for
HIF1AN

Expression for HIF1AN Gene

mRNA expression in normal human tissues for HIF1AN Gene

mRNA differential expression in normal tissues according to GTEx for HIF1AN Gene

This gene is overexpressed in Muscle - Skeletal (x4.3).

Protein differential expression in normal tissues from HIPED for HIF1AN Gene

This gene is overexpressed in Peripheral blood mononuclear cells (9.5) and Fetal Brain (7.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for HIF1AN Gene



SOURCE GeneReport for Unigene cluster for HIF1AN Gene Hs.500788

genes like me logo Genes that share expression patterns with HIF1AN: view

Protein tissue co-expression partners for HIF1AN Gene

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for HIF1AN Gene

Orthologs for HIF1AN Gene

This gene was present in the common ancestor of chordates.

Orthologs for HIF1AN Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia HIF1AN 35
  • 93.87 (n)
  • 98.22 (a)
HIF1AN 36
  • 97 (a)
OneToOne
dog
(Canis familiaris)
Mammalia HIF1AN 35
  • 94.46 (n)
  • 97.42 (a)
HIF1AN 36
  • 97 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Hif1an 35
  • 91.12 (n)
  • 97.13 (a)
Hif1an 16
Hif1an 36
  • 97 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia HIF1AN 35
  • 99.81 (n)
  • 99.71 (a)
HIF1AN 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Hif1an 35
  • 91.12 (n)
  • 97.13 (a)
oppossum
(Monodelphis domestica)
Mammalia HIF1AN 36
  • 94 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia HIF1AN 36
  • 90 (a)
OneToOne
chicken
(Gallus gallus)
Aves HIF1AN 35
  • 80.83 (n)
  • 89.68 (a)
HIF1AN 36
  • 89 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia HIF1AN 36
  • 86 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia hif1an 35
  • 77.44 (n)
  • 87.01 (a)
MGC76135 35
zebrafish
(Danio rerio)
Actinopterygii Dr.13371 35
hif1an 35
  • 75.08 (n)
  • 84.31 (a)
hif1an 36
  • 80 (a)
OneToOne
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.13324 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 57 (a)
OneToOne
Species with no ortholog for HIF1AN:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for HIF1AN Gene

ENSEMBL:
Gene Tree for HIF1AN (if available)
TreeFam:
Gene Tree for HIF1AN (if available)

Paralogs for HIF1AN Gene

Paralogs for HIF1AN Gene

genes like me logo Genes that share paralogs with HIF1AN: view

Variants for HIF1AN Gene

Sequence variations from dbSNP and Humsavar for HIF1AN Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type MAF
rs11292 -- 100,553,850(-) CATTG(C/T)TTCAG utr-variant-3-prime
rs1044906 -- 100,549,926(+) GGGTT(C/T)TCTGA utr-variant-3-prime
rs1044908 -- 100,549,928(+) GTTCT(C/T)TGATG utr-variant-3-prime
rs1054399 -- 100,552,808(+) TTTTG(C/T)CCATG utr-variant-3-prime
rs1057806 -- 100,548,353(+) GCCCC(A/T)GCACC utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for HIF1AN Gene

Variant ID Type Subtype PubMed ID
essv24916 CNV CNV 17122850
nsv831962 CNV Gain 17160897
dgv811n71 CNV Loss 21882294
nsv831963 CNV Loss 17160897
nsv895957 CNV Gain 21882294
nsv7519 CNV Insertion 18451855

Variation tolerance for HIF1AN Gene

Residual Variation Intolerance Score: 43.02% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.97; 79.93% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for HIF1AN Gene

HapMap Linkage Disequilibrium report
HIF1AN
Human Gene Mutation Database (HGMD)
HIF1AN

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HIF1AN Gene

Disorders for HIF1AN Gene

MalaCards: The human disease database

(3) MalaCards diseases for HIF1AN Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
hypoxia
severe pre-eclampsia
  • severe pre-eclampsia, with delivery
acute mountain sickness
  • altitude anoxia
- elite association

Relevant External Links for HIF1AN

Genetic Association Database (GAD)
HIF1AN
Human Genome Epidemiology (HuGE) Navigator
HIF1AN
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
HIF1AN
genes like me logo Genes that share disorders with HIF1AN: view

No data available for UniProtKB/Swiss-Prot and Genatlas for HIF1AN Gene

Publications for HIF1AN Gene

  1. FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity. (PMID: 11641274) Mahon P.C. … Semenza G.L. (Genes Dev. 2001) 2 23 67
  2. Identification and characterization of the hypoxia-responsive element in human stanniocalcin-1 gene. (PMID: 19628018) Law A.Y. … Wong C.K. (Mol. Cell. Endocrinol. 2010) 23 67
  3. Crystal structures of human FIH-1 in complex with quinol family inhibitors. (PMID: 20396966) Moon H. … Choe J. (Mol. Cells 2010) 23 67
  4. Hinokitiol activates the hypoxia-inducible factor (HIF) pathway through inhibition of HIF hydroxylases. (PMID: 20416277) Lee M.J. … Yang E.G. (Biochem. Biophys. Res. Commun. 2010) 23 67
  5. Transcriptional activation of hypoxia-inducible factor-1alpha by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1. (PMID: 19071119) Seo H.W. … Lee M.O. (FEBS Lett. 2009) 23 67

Products for HIF1AN Gene

Sources for HIF1AN Gene

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