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Aliases for HIF1AN Gene

Aliases for HIF1AN Gene

  • Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor 2 3 5
  • Hypoxia-Inducible Factor Asparagine Hydroxylase 3 4
  • Peptide-Aspartate Beta-Dioxygenase 2 3
  • Factor Inhibiting HIF-1 3 4
  • FIH-1 3 4
  • FIH1 3 4
  • Hypoxia-Inducible Factor 1-Alpha Inhibitor 3
  • Factor Inhibiting HIF1 3
  • EC 1.14.11.30 4
  • EC 1.14.11.n4 4

External Ids for HIF1AN Gene

Previous GeneCards Identifiers for HIF1AN Gene

  • GC10P101188
  • GC10P101529
  • GC10P102420
  • GC10P101960
  • GC10P102285
  • GC10P095924

Summaries for HIF1AN Gene

GeneCards Summary for HIF1AN Gene

HIF1AN (Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) is a Protein Coding gene. Diseases associated with HIF1AN include Hypoxia and Anemia Of Prematurity. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and HIF-2-alpha transcription factor network. GO annotations related to this gene include protein homodimerization activity and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors. An important paralog of this gene is HSPBAP1.

UniProtKB/Swiss-Prot for HIF1AN Gene

  • Hydroxylates HIF-1 alpha at Asp-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Gene Wiki entry for HIF1AN Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HIF1AN Gene

Genomics for HIF1AN Gene

Regulatory Elements for HIF1AN Gene

Enhancers for HIF1AN Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH10G100369 1.9 FANTOM5 ENCODE dbSUPER 10.5 -156.6 -156629 6.8 CREB3L1 MLX AGO1 ZFP64 DMAP1 YBX1 FEZF1 YY1 SLC30A9 ZNF143 PHBP9 SEC31B EBAG9P1 SCD ENSG00000231188 ENSG00000272572 CHUK TWNK ENSG00000255339 NDUFB8
GH10G100481 1.3 Ensembl ENCODE 11.1 -46.6 -46648 2.0 PKNOX1 CREB3L1 ARID4B SIN3A ZNF2 ZNF143 FOS ZNF263 SP3 NFYC SEC31B ENSG00000272572 NDUFB8 ENSG00000255339 HIF1AN LOC105378452 GC10M100500
GH10G100339 1.2 Ensembl ENCODE dbSUPER 10.1 -189.8 -189794 1.0 SOX5 GATAD2A CTCF BHLHE40 TRIM22 DPF2 TBX21 RAD21 FOXA1 FOXK2 PKD2L1 SEC31B HIF1AN NDUFB8 ENSG00000255339 ENSG00000231188 BLOC1S2
GH10G100434 1 ENCODE 10.4 -95.1 -95114 0.2 HDGF ATF1 ARNT ARID4B SIN3A ZNF2 ZNF48 SLC30A9 ZNF143 DEK SEC31B SCD ENSG00000231188 WNT8B ENSG00000255339 NDUFB8 HIF1AN ENSG00000273030
GH10G100338 1 ENCODE dbSUPER 10.1 -190.4 -190444 0.2 CTCF FOXA2 ARNT ARID4B RAD21 RFX5 FOXK2 ZNF143 ZNF207 SMC3 SEC31B NDUFB8 HIF1AN ENSG00000255339 ENSG00000231188 BLOC1S2
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around HIF1AN on UCSC Golden Path with GeneCards custom track

Promoters for HIF1AN Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000032517 428 1401 PKNOX1 ATF1 ZFP64 WRNIP1 SIN3A DMAP1 YY1 ETS1 ZNF121 DEK

Genomic Location for HIF1AN Gene

Chromosome:
10
Start:
100,529,072 bp from pter
End:
100,559,998 bp from pter
Size:
30,927 bases
Orientation:
Plus strand

Genomic View for HIF1AN Gene

Genes around HIF1AN on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
HIF1AN Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for HIF1AN Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for HIF1AN Gene

Proteins for HIF1AN Gene

  • Protein details for HIF1AN Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NWT6-HIF1N_HUMAN
    Recommended name:
    Hypoxia-inducible factor 1-alpha inhibitor
    Protein Accession:
    Q9NWT6
    Secondary Accessions:
    • D3DR69
    • Q5W147
    • Q969Q7
    • Q9NPV5

    Protein attributes for HIF1AN Gene

    Size:
    349 amino acids
    Molecular mass:
    40285 Da
    Cofactor:
    Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
    Quaternary structure:
    • Homodimer; homodimerization is essential for catalytic activity. Interacts with VHL and HIF1A. Part of a complex with VHL, HIF1A and HDAC1 or HDAC2 or HDAC3. Interacts with NFKB1 and NFKBIA. Interacts with NOTCH1, NOTCH2 and NOTCH3 but not with NOTCH4. Interacts with APBA3; binding inhibits HIF1AN binding to HIF1A. Interacts with TNKS2. Interacts with PPP1R12A. Interacts with ASB4 (By similarity). Interacts with UBE3A. Interacts with ANKS3 (By similarity).

    Three dimensional structures from OCA and Proteopedia for HIF1AN Gene

neXtProt entry for HIF1AN Gene

Post-translational modifications for HIF1AN Gene

  • Ubiquitination at isoforms=115 and posLast=131131
  • Modification sites at PhosphoSitePlus

Other Protein References for HIF1AN Gene

Antibody Products

  • R&D Systems Antibodies for HIF1AN (FIH-1/HIF-1AN)
  • Cell Signaling Technology (CST) Antibodies for HIF1AN (HIF1AN)

No data available for DME Specific Peptides for HIF1AN Gene

Domains & Families for HIF1AN Gene

Protein Domains for HIF1AN Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for HIF1AN Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with HIF1AN: view

No data available for Gene Families and UniProtKB/Swiss-Prot for HIF1AN Gene

Function for HIF1AN Gene

Molecular function for HIF1AN Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=100 uM for HIF1A (788-822) peptide {ECO:0000269 PubMed:14701857}; KM=160 uM for HIF2A (832-866) peptide {ECO:0000269 PubMed:14701857}; KM=0.5 uM for Fe(2+) {ECO:0000269 PubMed:14701857}; KM=25 uM for 2-oxoglutarate {ECO:0000269 PubMed:14701857}; KM=260 uM for ascorbate {ECO:0000269 PubMed:14701857}; KM=90 uM for O(2) {ECO:0000269 PubMed:14701857}; Note=The kinetic constants are determined for the recombinant FLAG-His-tagged protein.;
UniProtKB/Swiss-Prot CatalyticActivity:
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2).
UniProtKB/Swiss-Prot CatalyticActivity:
Ankyrin-repeat-L-histidine + 2-oxoglutarate + O(2) = ankyrin-repeat-(3S)-3-hydroxy-L-histidine + succinate + CO(2).
UniProtKB/Swiss-Prot Function:
Hydroxylates HIF-1 alpha at Asp-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Enzyme Numbers (IUBMB) for HIF1AN Gene

Gene Ontology (GO) - Molecular Function for HIF1AN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005112 Notch binding IPI 17573339
GO:0005506 iron ion binding IDA 12446723
GO:0005515 protein binding IPI 11641274
GO:0008270 zinc ion binding IDA 12446723
GO:0016491 oxidoreductase activity IEA --
genes like me logo Genes that share ontologies with HIF1AN: view
genes like me logo Genes that share phenotypes with HIF1AN: view

Animal Models for HIF1AN Gene

MGI Knock Outs for HIF1AN:

Animal Model Products

  • Taconic Biosciences Mouse Models for HIF1AN

CRISPR Products

miRNA for HIF1AN Gene

miRTarBase miRNAs that target HIF1AN

Inhibitory RNA Products

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for HIF1AN Gene

Localization for HIF1AN Gene

Subcellular locations from UniProtKB/Swiss-Prot for HIF1AN Gene

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for HIF1AN gene
Compartment Confidence
nucleus 5
cytosol 5
mitochondrion 2

Gene Ontology (GO) - Cellular Components for HIF1AN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 colocalizes_with nucleus IEA,IDA 18299578
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA,IDA 14734545
GO:0005829 cytosol TAS --
GO:0048471 perinuclear region of cytoplasm IEA,IDA 19726677
genes like me logo Genes that share ontologies with HIF1AN: view

Pathways & Interactions for HIF1AN Gene

genes like me logo Genes that share pathways with HIF1AN: view

SIGNOR curated interactions for HIF1AN Gene

Inactivates:

Gene Ontology (GO) - Biological Process for HIF1AN Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0036138 peptidyl-histidine hydroxylation IDA 21251231
GO:0042264 peptidyl-aspartic acid hydroxylation IDA 21177872
GO:0042265 peptidyl-asparagine hydroxylation IMP 19245366
genes like me logo Genes that share ontologies with HIF1AN: view

Drugs & Compounds for HIF1AN Gene

(10) Drugs for HIF1AN Gene - From: DrugBank, ApexBio, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Oxygen Approved, Vet_approved Pharma 0
Tartaric acid Approved Pharma Enzyme, catalytic activity 0
Carbon dioxide Approved, Vet_approved Pharma 0
Succinic acid Approved Nutra Full agonist, Agonist 0
D-tartaric acid Experimental Pharma Target 0

(5) Additional Compounds for HIF1AN Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Fe2+
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
15438-31-0
Oxoglutaric acid
  • 2-Ketoglutarate
  • 2-Ketoglutaric acid
  • 2-Oxo-1,5-pentanedioate
  • 2-Oxo-1,5-pentanedioic acid
  • 2-Oxoglutarate
328-50-7

(1) ApexBio Compounds for HIF1AN Gene

Compound Action Cas Number
BAY 87-2243 HIF-1 inhibitor,potent and selective 1227158-85-1
genes like me logo Genes that share compounds with HIF1AN: view

Drug Products

Transcripts for HIF1AN Gene

Unigene Clusters for HIF1AN Gene

Hypoxia inducible factor 1, alpha subunit inhibitor:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for HIF1AN Gene

No ASD Table

Relevant External Links for HIF1AN Gene

GeneLoc Exon Structure for
HIF1AN
ECgene alternative splicing isoforms for
HIF1AN

Expression for HIF1AN Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for HIF1AN Gene

mRNA differential expression in normal tissues according to GTEx for HIF1AN Gene

This gene is overexpressed in Muscle - Skeletal (x4.3).

Protein differential expression in normal tissues from HIPED for HIF1AN Gene

This gene is overexpressed in Peripheral blood mononuclear cells (9.5) and Fetal Brain (7.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for HIF1AN Gene



Protein tissue co-expression partners for HIF1AN Gene

NURSA nuclear receptor signaling pathways regulating expression of HIF1AN Gene:

HIF1AN

SOURCE GeneReport for Unigene cluster for HIF1AN Gene:

Hs.500788

Evidence on tissue expression from TISSUES for HIF1AN Gene

  • Nervous system(4.6)
  • Muscle(4.3)
genes like me logo Genes that share expression patterns with HIF1AN: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for HIF1AN Gene

Orthologs for HIF1AN Gene

This gene was present in the common ancestor of chordates.

Orthologs for HIF1AN Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia HIF1AN 34 35
  • 99.81 (n)
dog
(Canis familiaris)
Mammalia HIF1AN 34 35
  • 94.46 (n)
oppossum
(Monodelphis domestica)
Mammalia HIF1AN 35
  • 94 (a)
OneToOne
cow
(Bos Taurus)
Mammalia HIF1AN 34 35
  • 93.87 (n)
mouse
(Mus musculus)
Mammalia Hif1an 34 16 35
  • 91.12 (n)
rat
(Rattus norvegicus)
Mammalia Hif1an 34
  • 91.12 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia HIF1AN 35
  • 90 (a)
OneToOne
chicken
(Gallus gallus)
Aves HIF1AN 34 35
  • 80.83 (n)
lizard
(Anolis carolinensis)
Reptilia HIF1AN 35
  • 86 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia hif1an 34
  • 77.44 (n)
MGC76135 34
zebrafish
(Danio rerio)
Actinopterygii hif1an 34 35
  • 75.08 (n)
Dr.13371 34
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 57 (a)
OneToOne
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.13324 34
Species where no ortholog for HIF1AN was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for HIF1AN Gene

ENSEMBL:
Gene Tree for HIF1AN (if available)
TreeFam:
Gene Tree for HIF1AN (if available)

Paralogs for HIF1AN Gene

Paralogs for HIF1AN Gene

genes like me logo Genes that share paralogs with HIF1AN: view

Variants for HIF1AN Gene

Sequence variations from dbSNP and Humsavar for HIF1AN Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type
rs1000149170 -- 100,551,526(+) AGTTG(A/C)CAGCC utr-variant-3-prime
rs1000184123 -- 100,534,006(+) CTGCA(C/T)CTCCC upstream-variant-2KB
rs1000369682 -- 100,551,909(+) CTACC(C/T)AGGCT utr-variant-3-prime
rs1000408468 -- 100,546,232(+) CCCTG(C/T)CTCTA intron-variant
rs1000593922 -- 100,535,759(+) GAGCC(A/T)AGGAT upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for HIF1AN Gene

Variant ID Type Subtype PubMed ID
dgv277e212 CNV loss 25503493
esv2759779 CNV gain+loss 17122850
nsv7519 CNV insertion 18451855
nsv831962 CNV gain 17160897
nsv831963 CNV loss 17160897

Variation tolerance for HIF1AN Gene

Residual Variation Intolerance Score: 43% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.97; 79.93% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for HIF1AN Gene

Human Gene Mutation Database (HGMD)
HIF1AN
SNPedia medical, phenotypic, and genealogical associations of SNPs for
HIF1AN

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HIF1AN Gene

Disorders for HIF1AN Gene

MalaCards: The human disease database

(5) MalaCards diseases for HIF1AN Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
hypoxia
anemia of prematurity
acute mountain sickness
  • altitude anoxia
severe pre-eclampsia
  • antepartum severe pre-eclampsia
pre-eclampsia
  • preeclampsia
- elite association - COSMIC cancer census association via MalaCards

Relevant External Links for HIF1AN

Genetic Association Database (GAD)
HIF1AN
Human Genome Epidemiology (HuGE) Navigator
HIF1AN
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
HIF1AN
genes like me logo Genes that share disorders with HIF1AN: view

No data available for UniProtKB/Swiss-Prot and Genatlas for HIF1AN Gene

Publications for HIF1AN Gene

  1. FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity. (PMID: 11641274) Mahon P.C. … Semenza G.L. (Genes Dev. 2001) 2 3 4 22 64
  2. Mint3 enhances the activity of hypoxia-inducible factor-1 (HIF-1) in macrophages by suppressing the activity of factor inhibiting HIF-1. (PMID: 19726677) Sakamoto T. … Seiki M. (J. Biol. Chem. 2009) 3 4 22 64
  3. Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways. (PMID: 18299578) Zheng X. … Poellinger L. (Proc. Natl. Acad. Sci. U.S.A. 2008) 3 4 22 64
  4. Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor. (PMID: 14734545) Linke S. … Peet D.J. (J. Biol. Chem. 2004) 3 4 22 64
  5. Structure of human FIH-1 reveals a unique active site pocket and interaction sites for HIF-1 and von Hippel-Lindau. (PMID: 12482756) Lee C. … Ryu S.E. (J. Biol. Chem. 2003) 3 4 22 64

Products for HIF1AN Gene

Sources for HIF1AN Gene

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