Free for academic non-profit institutions. Other users need a Commercial license

HERC5 Gene(Protein Coding)

HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5

GCID:
GC04P088457
GIFtS:
54
Genes Participants
UDN Logo

Aliases for HERC5 Gene

Aliases for HERC5 Gene

  • HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5 2 3 5
  • HECT Domain And RCC1-Like Domain-Containing Protein 5 3 4
  • Cyclin-E-Binding Protein 1 3 4
  • Hect Domain And RLD 5 2 3
  • CEBP1 3 4
  • CEB1 3 4
  • Probable E3 Ubiquitin-Protein Ligase HERC5 3
  • E3 ISG15--Protein Ligase HERC5 3
  • EC 2.3.2.- 4
  • EC 6.3.2 61

External Ids for HERC5 Gene

Previous GeneCards Identifiers for HERC5 Gene

  • GC04P089837
  • GC04P089735
  • GC04P089597
  • GC04P089378
  • GC04P085130

Summaries for HERC5 Gene

Entrez Gene Summary for HERC5 Gene

  • This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

GeneCards Summary for HERC5 Gene

HERC5 (HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5) is a Protein Coding gene. Among its related pathways are Interferon gamma signaling and Innate Immune System. GO annotations related to this gene include poly(A) RNA binding and ubiquitin-protein transferase activity. An important paralog of this gene is HERC6.

UniProtKB/Swiss-Prot for HERC5 Gene

  • Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.

Gene Wiki entry for HERC5 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for HERC5 Gene

Genomics for HERC5 Gene

Products:

  1. Regulatory Element

Regulatory Elements for HERC5 Gene

Enhancers for HERC5 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH04G088381 1.4 FANTOM5 Ensembl ENCODE 13.2 -74.9 -74874 2.3 TBP PKNOX1 SIN3A FEZF1 ZNF2 ZNF366 CBX5 RELB IKZF2 ZEB2 HERC6 HERC5 GC04M088341
GH04G088455 1.7 FANTOM5 Ensembl ENCODE 2.6 -0.4 -384 2.8 HDGF PKNOX1 FOXA2 WRNIP1 ARID4B SIN3A FEZF1 ZNF2 YY1 SP3 HERC6 HERC5 GC04M088454
GH04G088791 1.5 FANTOM5 Ensembl ENCODE dbSUPER 1.8 +335.6 335559 2.4 CTCF PKNOX1 MTA2 L3MBTL2 NR2F1 HLF DPF2 RAD21 IKZF1 STAT3 FAM13A-AS1 NAP1L5 FAM13A HERC6 HERC5 PIR43353 LOC105377327
GH04G088177 1.3 FANTOM5 Ensembl ENCODE 1.9 -278.6 -278601 1.7 TEAD4 MAX BACH1 CEBPG RBBP5 TEAD3 ZNF316 FOS NFE2 MAFK LOC105369192 HERC6 HERC5 RPL31P24 RNU6-818P
GH04G088512 0.3 FANTOM5 1.6 +55.6 55612 0.4 HERC5 GC04M088495 NCOA4P2
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around HERC5 on UCSC Golden Path with GeneCards custom track

Promoters for HERC5 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000170578 -17 1801 HDGF PKNOX1 FOXA2 WRNIP1 ARID4B SIN3A FEZF1 ZNF2 YY1 FOS

Transcription factor binding sites by QIAGEN in the HERC5 gene promoter:

Genomic Location for HERC5 Gene

Chromosome:
4
Start:
88,457,117 bp from pter
End:
88,506,170 bp from pter
Size:
49,054 bases
Orientation:
Plus strand

Genomic View for HERC5 Gene

Genes around HERC5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
HERC5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for HERC5 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for HERC5 Gene

Proteins for HERC5 Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for HERC5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UII4-HERC5_HUMAN
    Recommended name:
    E3 ISG15--protein ligase HERC5
    Protein Accession:
    Q9UII4
    Secondary Accessions:
    • B2RTQ1
    • Q69G20

    Protein attributes for HERC5 Gene

    Size:
    1024 amino acids
    Molecular mass:
    116852 Da
    Quaternary structure:
    • Binds to CCNA1, CCNB1, CCND1 and CCNE1. Interacts with UBE2L6. Interacts with IRF3, this interaction is marginal in resting cells but enhanced upon viral infection. Interacts with influenza A virus NS1.

neXtProt entry for HERC5 Gene

Protein Expression for HERC5 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for HERC5 Gene

Q9UII4:
  • GQLGHNST
  • YHSLALSK

Post-translational modifications for HERC5 Gene

Other Protein References for HERC5 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for HERC5 Gene

Protein Domains for HERC5 Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for HERC5 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with HERC5: view

No data available for Gene Families and UniProtKB/Swiss-Prot for HERC5 Gene

Function for HERC5 Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line
  7. Flow Cytometry

Molecular function for HERC5 Gene

UniProtKB/Swiss-Prot Function:
Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
UniProtKB/Swiss-Prot Induction:
By IFNB1/IFN-beta. In endothelial cells, by TNF, IL1B/interleukin-1B and by bacterial lipopolysaccharides (LPS), hardly induced in other cells of the vascular wall such as primary smooth muscle cells and fibroblasts. By viral infection.

Enzyme Numbers (IUBMB) for HERC5 Gene

Gene Ontology (GO) - Molecular Function for HERC5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IDA 22658674
GO:0004842 ubiquitin-protein transferase activity IEA,TAS --
GO:0005515 protein binding IPI 20308324
GO:0016740 transferase activity IEA --
GO:0042296 ISG15 transferase activity IDA 20308324
genes like me logo Genes that share ontologies with HERC5: view
genes like me logo Genes that share phenotypes with HERC5: view

Animal Model Products

CRISPR Products

miRNA for HERC5 Gene

miRTarBase miRNAs that target HERC5

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for HERC5 Gene

Localization for HERC5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for HERC5 Gene

Cytoplasm, perinuclear region. Note=Associated with the polyribosomes, probably via the 60S subunit.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for HERC5 gene
Compartment Confidence
cytosol 5
nucleus 3

Gene Ontology (GO) - Cellular Components for HERC5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
GO:0048471 perinuclear region of cytoplasm IEA --
genes like me logo Genes that share ontologies with HERC5: view

Pathways & Interactions for HERC5 Gene

genes like me logo Genes that share pathways with HERC5: view

Gene Ontology (GO) - Biological Process for HERC5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity TAS 10581175
GO:0000209 protein polyubiquitination TAS --
GO:0002376 immune system process IEA --
GO:0016567 protein ubiquitination IEA --
GO:0032020 ISG15-protein conjugation IDA 20308324
genes like me logo Genes that share ontologies with HERC5: view

No data available for SIGNOR curated interactions for HERC5 Gene

Drugs & Compounds for HERC5 Gene

Products:

  1. Drug
No Compound Related Data Available

Transcripts for HERC5 Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
  5. Flow Cytometry

mRNA/cDNA for HERC5 Gene

(2) REFSEQ mRNAs :
(4) Additional mRNA sequences :
(54) Selected AceView cDNA sequences:
(5) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for HERC5 Gene

HECT and RLD domain containing E3 ubiquitin protein ligase 5:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for HERC5 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24a ·
SP1: - - -
SP2:
SP3: - -
SP4: -
SP5:

ExUns: 24b ^ 25 ^ 26
SP1:
SP2:
SP3:
SP4:
SP5:

Relevant External Links for HERC5 Gene

GeneLoc Exon Structure for
HERC5
ECgene alternative splicing isoforms for
HERC5

Expression for HERC5 Gene

Products:

  1. Primer

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for HERC5 Gene

mRNA expression in normal human tissues for HERC5 Gene
mRNA expression by BioGPS for HERC5 GenemRNA expression by GTEx for HERC5 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for HERC5 Gene

This gene is overexpressed in Testis (x7.8).

Protein differential expression in normal tissues from HIPED for HERC5 Gene

This gene is overexpressed in Fetal ovary (28.0), Ovary (20.2), and Testis (18.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for HERC5 Gene



Protein tissue co-expression partners for HERC5 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of HERC5 Gene:

HERC5

SOURCE GeneReport for Unigene cluster for HERC5 Gene:

Hs.26663

mRNA Expression by UniProt/SwissProt for HERC5 Gene:

Q9UII4-HERC5_HUMAN
Tissue specificity: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Evidence on tissue expression from TISSUES for HERC5 Gene

  • Nervous system(4.1)
genes like me logo Genes that share expression patterns with HERC5: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for HERC5 Gene

Orthologs for HERC5 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for HERC5 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia HERC5 34 35
  • 96.21 (n)
dog
(Canis familiaris)
 Mammalia HERC5 34 35
  • 84.79 (n)
cow
(Bos Taurus)
 Mammalia HERC5 34 35
  • 80.8 (n)
lizard
(Anolis carolinensis)
 Reptilia HERC5 35
  • 32 (a)
 OneToOne
zebrafish
(Danio rerio)
 Actinopterygii HERC5 (3 of 4) 35
  • 32 (a)
 OneToMany
HERC5 (4 of 4) 35
  • 32 (a)
 OneToMany
HERC5 (1 of 4) 35
  • 31 (a)
 OneToMany
HERC5 (2 of 4) 35
  • 30 (a)
 OneToMany
fruit fly
(Drosophila melanogaster)
 Insecta CG6678 35
  • 19 (a)
 OneToMany
worm
(Caenorhabditis elegans)
 Secernentea K11D2.1 35
  • 21 (a)
 OneToMany
baker's yeast
(Saccharomyces cerevisiae)
 Saccharomycetes ATS1 37
sea squirt
(Ciona savignyi)
 Ascidiacea -- 35
  • 25 (a)
 OneToMany
Species where no ortholog for HERC5 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • mouse (Mus musculus)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for HERC5 Gene

ENSEMBL:
Gene Tree for HERC5 (if available)
TreeFam:
Gene Tree for HERC5 (if available)

Paralogs for HERC5 Gene

Paralogs for HERC5 Gene

(6) SIMAP similar genes for HERC5 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with HERC5: view

Variants for HERC5 Gene

Sequence variations from dbSNP and Humsavar for HERC5 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs1000032832 -- 88,479,608(+) CATAC(A/G)AAGTT intron-variant
rs1000046443 -- 88,486,036(+) TTTAA(A/T)CTGAT intron-variant
rs1000115332 -- 88,478,478(+) ACACA(A/G)AGAAG intron-variant
rs1000176425 -- 88,479,647(+) AAAAT(C/T)ACATC intron-variant
rs1000189812 -- 88,499,247(+) TTACA(A/T)CCCCT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for HERC5 Gene

Variant ID Type Subtype PubMed ID
nsv1112857 CNV deletion 24896259

Variation tolerance for HERC5 Gene

Residual Variation Intolerance Score: 20.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.31; 62.96% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for HERC5 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
HERC5

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for HERC5 Gene

Disorders for HERC5 Gene

Relevant External Links for HERC5

Genetic Association Database (GAD)
HERC5
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
HERC5

No disorders were found for HERC5 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for HERC5 Gene

Publications for HERC5 Gene

  1. HERC5 is an IFN-induced HECT-type E3 protein ligase that mediates type I IFN-induced ISGylation of protein targets. (PMID: 16815975) Wong J.J. … Chin K.C. (Proc. Natl. Acad. Sci. U.S.A. 2006) 3 4 22 64
  2. HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS activated endothelial cells. (PMID: 15331633) Kroismayr R. … Lipp J. (J. Cell Sci. 2004) 3 4 22 64
  3. A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins. (PMID: 10581175) Mitsui K. … Ohtsubo M. (Biochem. Biophys. Res. Commun. 1999) 2 3 4 64
  4. Genome-wide association study of lung function decline in adults with and without asthma. (PMID: 22424883) Imboden M. … Probst-Hensch N.M. (J. Allergy Clin. Immunol. 2012) 3 46 64
  5. Positive regulation of interferon regulatory factor 3 activation by Herc5 via ISG15 modification. (PMID: 20308324) Shi H.X. … Wang C. (Mol. Cell. Biol. 2010) 3 4 64

Products for HERC5 Gene

Sources for HERC5 Gene

Content
Loading form....