HELB Gene
protein-coding GIFtS: 45
GCID: GC12P066696
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|
helicase (DNA) B
| |
Aliases
for HELB gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Helicase (DNA) B1 2 | | HDHB1 | | DNA Helicase B2 | | Helicase B2 | | EC 3.6.4.123 |
Export aliases for HELB gene to outside databasesPrevious GC identifers: GC12P066087 GC00U907484 GC12P066413 GC12P064982 GC12P063747 |
Summaries
for HELB gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for HELB:
This gene encodes a DNA-dependent ATPase which catalyzes the unwinding of DNA necessary for DNA replication, repair,recombination, and transcription. This gene is thought to function specifically during the S phase entry of the cellcycle. (provided by RefSeq, Mar 2012)
UniProtKB/Swiss-Prot: HELB_HUMAN, Q8NG08Function: Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities.Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress
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Genomic Views
for HELB gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000012.11 NC_018923.1 NT_029419.12
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the HELB gene promoter:
Max1 Lmo2 Ik-3 Arnt GATA-6 POU2F1 HFH-1 POU2F1a FOXO1a FOXO1
Other transcription factors
Search SABiosciences Chromatin IP Primers for HELB
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HELB |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 12q14.3|12q Ensembl cytogenetic band: 12q14.3 HGNC cytogenetic band: 12q14.2HELB Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 12 GeneLoc Exon Structure GeneLoc location for GC12P066696: view genomic region
(about GC identifiers)
Start:
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66,696,325 bp from pter |
End:
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66,737,423 bp from pter |
Size:
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41,099 bases |
Orientation:
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plus strand |
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Proteins
for HELB gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: HELB_HUMAN, Q8NG08 (See
protein sequence)Recommended Name: DNA helicase B Size: 1087 amino acids; 123252 Da
Subunit: Binds to RPA1; this interaction promotes HELB recruitment to chromatin following DNA damage. Interacts with atleast two subunits of pol-prim
Subcellular location: Note=Preferentially during S phase, recruited to chromatin following DNA damage induced by UVirradiation, or camptothecin or hydroxyurea treatment
Sequence caution: Sequence=BAB15754.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: A8K4C9 Q4G0T2 Q9H7L5Alternative splicing: 2 isoforms: Q8NG08-1 Q8NG08-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for HELB: NX_Q8NG08
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8NG08
HELB Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_387467.2
ENSEMBL proteins: ENSP00000247815 ENSP00000396955 ENSP00000439617 ENSP00000443287
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005658 | colocalizes with alpha DNA polymerase:primase complex |
IDA | 12181327 |
HELB for ontologies About GeneDecksing
HELB Antibody Products:
Assay Products for HELB: |
Protein
Domains /
Families
for HELB gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
HELB for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q8NG08ProtoNet protein and cluster: Q8NG08 |
Function
for HELB gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: HELB_HUMAN, Q8NG08Function: Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities.Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stressCatalytic activity: ATP + H(2)O = ADP + phosphateEnzyme regulation: Inhibited by salt concentration greater than 100 mM. Uses either magnesium or manganese ions tosupport helicase activity. Binds strongly to single-stranded DNA in the absence of ATP but dissociates readily in thepresence of 1 mM ATPEnzyme Number (IUBMB): EC 3.6.4.121
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HELB (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HELB
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for HELB | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HELB |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HELB |
Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table
HELB for ontologies About GeneDecksing
|
Pathways & Interactions
for HELB gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for HELB
STRING Interaction
Network Preview (showing 4 interactants - click image to see more details)
4 Interacting proteins for HELB (Q8NG083 ENSP000002478154) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
HELB for ontologies About GeneDecksing
|
Drugs & Compounds
for HELB gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for HELB
2 HMDB Compounds for HELB About this table
Search CenterWatch for drugs/clinical trials and news about HELB
|
Transcripts
for HELB gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for HELB gene: NM_033647.3 Unigene Cluster for HELB: Helicase (DNA) B Hs.505941 [show with all ESTs]Unigene Representative Sequence: NM_0336476 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000247815 ENST00000440906 ENST00000542394(uc010ssz.1) ENST00000545134(uc001sti.2 uc009zqt.1)
ENST00000545455 ENST00000536862
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HELB (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HELB
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: HELB (NM_033647) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for HELB | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HELB |
Additional cDNA sequence: AF319995.1 AK290894.1 BC143409.1 BC143411.1 4 DOTS entries: DT.100017201 DT.100657040 DT.40308642 DT.75135008 18 AceView cDNA sequences: AI224503 AA256278 AA256818 NM_033647 BU195661 AL602198 BI596828 BX510230 BX096922 BX641439 AW003565 BX510066 BE778166 BC037946 AA070301 BF380938 AA070216 AK024464
GeneLoc Exon Structure
|
Expression
for HELB gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| HELB expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GAAATTTTCT
About this image
See HELB Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for HELB
SOURCE GeneReport for Unigene cluster: Hs.505941
UniProtKB/Swiss-Prot: HELB_HUMAN, Q8NG08Tissue specificity: Highly expressed in testis and thymus and weakly in liver, spleen, kidney and brain
SABiosciences Custom PCR Arrays for HELB
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HELB
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat HELB | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HELB | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HELB | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HELB |
Orthologs
for HELB gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for HELB gene from 4/12 species (see all 12) About this table
ENSEMBL Gene Tree for HELB (if available)
TreeFam Gene Tree for HELB (if available) |
Paralogs
for HELB gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for HELB gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 12 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for HELB (66696325 - 66737423 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for HELB: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HELB |
|
Disorders
/ Diseases
for HELB gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
HELB for disorders About GeneDecksing
OMIM gene information: 614539
OMIM disorders: --
2  diseases for HELB: About MalaCardsxeroderma pigmentosum liposarcoma
1 disease from the University of Copenhagen DISEASES database for HELB:Atypical lipomatous tumor Export disorders for HELB gene to outside databases
|
Publications
for HELB gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for HELB gene, integrated from 9 sources (see all 12):
(articles sorted by number of sources associating them with HELB) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication. (PubMed id 12181327)1, 2, 3 Taneja P.... Fanning E. (2002)
- Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress. (PubMed id 22194613)1, 2 Guler G.D.... Fanning E. (2012)
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (2009)
- ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. (PubMed id 17525332)2 Matsuoka S.... Elledge S.J. (2007)
- Characterization of the interactome of the human MutL homologues MLH1, PMS1, and PMS2. (PubMed id 17148452)1 Cannavo E....Jiricny J. (2007)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cells. (PubMed id 11557815)1 Tada S....Enomoto T. (2001)
|
External Searches for HELB gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing HELB gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing HELB gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing HELB gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for HELB | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for HELB gene:
Search GeneIP for patents involving HELB
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for HELB gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for HELB | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HELB | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HELB | | | OriGene Protein Over-expression Lysate for HELB | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for HELB | | OriGene 3'-UTR Clone for HELB | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HELB | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HELB | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for HELB | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for HELB | | OriGene Custom Protein Services for HELB | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat HELB | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HELB | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HELB | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HELB | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HELB | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HELB |
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 |  |  |  | |
| | | | Search Tocris compounds for HELB |
| |  |  |  |  | | | | |
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 | | | HELB Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HELB |
|  |  |  | | | | Search ThermoFisher Antibodies for HELB |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HELB |
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