HECTD2 Gene
protein-coding GIFtS: 47
GCID: GC10P093160
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HECT domain containing E3 ubiquitin protein ligase 2(Previous name: HECT domain containing 2 )
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Aliases
for HECTD2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| HECT Domain Containing E3 Ubiquitin Protein Ligase 21 2 | | HECT Domain Containing 21 2 | | HECT Domain-Containing Protein 22 3 | | FLJ373061 | | Probable E3 Ubiquitin-Protein Ligase HECTD22 | | EC 6.3.2.-3 |
Export aliases for HECTD2 gene to outside databasesPrevious GC identifers: GC10P092835 GC10P086798 |
Summaries
for HECTD2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: HECD2_HUMAN, Q5U5R9Function: Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in theform of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity)
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Genomic Views
for HECTD2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000010.10 NC_018921.1 NT_030059.13
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the HECTD2 gene promoter:
HOXA9 HOXA9B Gfi-1 Evi-1 POU2F1 POU2F1a Meis-1a Meis-1 RSRFC4
Other transcription factors
Search SABiosciences Chromatin IP Primers for HECTD2
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HECTD2 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 10q23.32 Ensembl cytogenetic band: 10q23.32 HGNC chromosome: 10HECTD2 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 10 GeneLoc Exon Structure GeneLoc location for GC10P093160: view genomic region
(about GC identifiers)
Start:
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93,170,096 bp from pter |
End:
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93,274,586 bp from pter |
Size:
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104,491 bases |
Orientation:
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plus strand |
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Proteins
for HECTD2 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: HECD2_HUMAN, Q5U5R9 (See
protein sequence)Recommended Name: Probable E3 ubiquitin-protein ligase HECTD2 Size: 776 amino acids; 88122 Da
Secondary accessions: Q5VZ97 Q5VZ98 Q5VZ99 Q8N1X7 Q8TCP5Alternative splicing: 2 isoforms: Q5U5R9-1 Q5U5R9-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for HECTD2: NX_Q5U5R9
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q5U5R9
HECTD2 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_775768.2 NP_877497.2
ENSEMBL proteins: ENSP00000360746 ENSP00000298068 ENSP00000360731 ENSP00000401023 ENSP00000439687
Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
HECTD2 for ontologies About GeneDecksing
HECTD2 Antibody Products:
Assay Products for HECTD2: |
Protein
Domains /
Families
for HECTD2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
HECTD2 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q5U5R9ProtoNet protein and cluster: Q5U5R9 1 Blocks protein family: IPB000569 HECT domain (Ubiquitin-protein ligase)
UniProtKB/Swiss-Prot: HECD2_HUMAN, Q5U5R9Similarity: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain |
Function
for HECTD2 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: HECD2_HUMAN, Q5U5R9Function: Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in theform of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity)Enzyme Number (IUBMB): EC 6.3.2.-1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HECTD2 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HECTD2 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for HECTD2 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HECTD2 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HECTD2 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004842 | ubiquitin-protein ligase activity |
IBA | -- | | GO:0016881 | acid-amino acid ligase activity |
-- | -- |
HECTD2 for ontologies About GeneDecksing
2 GenomeRNAi human phenotypes for HECTD2:
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Pathways & Interactions
for HECTD2 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: HECD2_HUMAN, Q5U5R9Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for HECTD2
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
5/16 Interacting proteins for HECTD2 (Q5U5R93 ENSP000002980684) via UniProtKB, MINT, STRING, and/or I2D (see all 16) About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006464 | cellular protein modification process |
-- | -- | | GO:0042787 | protein ubiquitination involved in ubiquitin-dependent protein catabolic process |
IBA | -- |
HECTD2 for ontologies About GeneDecksing
|
Drugs & Compounds
for HECTD2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for HECTD2
Search CenterWatch for drugs/clinical trials and news about HECTD2 / HECD2
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Transcripts
for HECTD2 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for HECTD2 gene (2 alternative transcripts): NM_173497.2 NM_182765.3 Unigene Cluster for HECTD2: HECT domain containing E3 ubiquitin protein ligase 2 Hs.596096 [show with all ESTs]Unigene Representative Sequence: NM_1827657 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000371681(uc001khk.2) ENST00000298068(uc009xty.1) ENST00000487511
ENST00000498446 ENST00000371667(uc001khn.1) ENST00000446394(uc001khl.2 uc010qnm.1 uc001khm.2)
ENST00000536715
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HECTD2 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HECTD2 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): HECTD2 (NM_182765) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for HECTD2 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HECTD2 |
Additional cDNA sequence: AF131793.1 AK094625.1 AK122629.1 AK295723.1 AL713675.1 BC040187.1 BX537856.1 8 DOTS entries: DT.95159826 DT.75100678 DT.40306043 DT.99955649 DT.410693 DT.100671974 DT.91948400 DT.92035613 24/80 AceView cDNA sequences (see all 80): AK122629 Z41325 AF131793 AA405558 AI640842 AL713675 AA633619 AL832073 Z45654 Z42993 AI217197 AW771211 BC036487 AI682502 AA405548 AA447223 AA744571 AI973069 AI635346 BX097208 D62490 AK094625 AW205041 NM_182765
GeneLoc Exon Structure
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Expression
for HECTD2 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| HECTD2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See HECTD2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for HECTD2
SOURCE GeneReport for Unigene cluster: Hs.596096
SABiosciences Custom PCR Arrays for HECTD2
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HECTD2
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat HECTD2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HECTD2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HECTD2 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HECTD2 |
Orthologs
for HECTD2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for HECTD2 gene from 4/17 species (see all 17) About this table
ENSEMBL Gene Tree for HECTD2 (if available)
TreeFam Gene Tree for HECTD2 (if available) |
Paralogs
for HECTD2 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for HECTD2 gene
- HERC12 HECTD32 HERC22 HERC32 RCCD12 HERC62 HERC42 UBE3A2
- HERC52
14 SIMAP similar genes for HECTD2 using alignment to 4 protein entries: HECD2_HUMAN (see all proteins):DKFZp313O1018 UBE3A DKFZp564G092 HERC3 HERC5 WWP2
HUWE1 NEDD4 HERC4 NEDD4L DKFZp564H223 HECW1
SMURF2 HECTD1
HECTD2 for paralogs About GeneDecksing
|
Genomic Variants
for HECTD2 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 10 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for HECTD2 (93170096 - 93274586 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 5 variations for HECTD2
3 CNVs: 85431 6894 37570
2 Inversions: 29427 37113 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HECTD2 |
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Disorders
/ Diseases
for HECTD2 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
HECTD2 for disorders About GeneDecksing
5  diseases for HECTD2: About MalaCardsbovine spongiform encephalopathy creutzfeldt-jakob syndrome prion disease alzheimer's disease
malaria
1 disease from the University of Copenhagen DISEASES database for HECTD2:Prion disease Human Genome Epidemiology (HuGE) Navigator: HECTD2 (2 documents)
Export disorders for HECTD2 gene to outside databases
|
Publications
for HECTD2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for HECTD2 gene, integrated from 9 sources (see all 14):
(articles sorted by number of sources associating them with HECTD2) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)1, 2 Deloukas P.... Rogers J. (2004)
- Large-scale concatenation cDNA sequencing. (PubMed id 9110174)1, 3 Yu W.... Gibbs R.A. (1997)
- A "double adaptor" method for improved shotgun library construction. (PubMed id 8619474)1, 3 Andersson B.... Gibbs R.A. (1996)
- Absence of association between two HECTD2 polymorphis ms and sporadic Creutzfeldt-Jakob disease. (PubMed id 21335971)1 Jeong B.H....Kim Y.S. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- HECTD2 is associated with susceptibility to mouse and human prion disease. (PubMed id 19214206)1 Lloyd S.E....Collinge J. (2009)
- HECTD2, a candidate susceptibility gene for Alzheimer 's disease on 10q. (PubMed id 19754925)1 Lloyd S.E....Collinge J. (2009)
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External Searches for HECTD2 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing HECTD2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing HECTD2 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing HECTD2 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for HECTD2 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for HECTD2 gene:
Search GeneIP for patents involving HECTD2
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for HECTD2 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for HECTD2 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for HECTD2 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for HECTD2 | | | OriGene Protein Over-expression Lysate for HECTD2 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for HECTD2 | | OriGene 3'-UTR Clone for HECTD2 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for HECTD2 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for HECTD2 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for HECTD2 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for HECTD2 | | OriGene Custom Protein Services for HECTD2 | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat HECTD2 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing HECTD2 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat HECTD2 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat HECTD2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat HECTD2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat HECTD2 |
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 | | | HECTD2 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for HECTD2 |
|  |  |  | | | | Search ThermoFisher Antibodies for HECTD2 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat HECTD2 |
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